LOCUS       pSCL31                  7091 bp    DNA     circular UNA 08-APR-2022
DEFINITION  Eco2 retron long primer.
ACCESSION   urn.local...3j-cl648gb
VERSION     urn.local...3j-cl648gb
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        127..231
                     /standard_name="AmpR promoter"
     CDS             232..300
                     /codon_start=1
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA"
                     /standard_name="AmpR"
     CDS             301..1092
                     /codon_start=1
                     /transl_table=1
                     /translation="HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMM
                     STFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAI
                     TMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVA
                     MATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSR
                     GIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /standard_name="AmpR"
     rep_origin      1263..1851
     promoter        1907..2267
                     /standard_name="pGal7"
     CDS             2274..>4031
                     /codon_start=1
                     /transl_table=11
                     /note="ORF4-Ec67 RT"
                     /db_xref="GOA:P21325"
                     /db_xref="InterPro:IPR000123"
                     /db_xref="InterPro:IPR000477"
                     /db_xref="UniProtKB/Swiss-Prot:P21325"
                     /protein_id="AAA23403.1"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="ORF4-Ec67 RT CDS"
     extention       4056..4070
                     /standard_name="long primer"
                     /modified_by="katecrawford"
                     /label="extended a1"
     misc_RNA        4071..4128
                     /standard_name="msr"
     motif           4072..4084
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="primer"
                     /modified_by="katecrawford"
                     /label="a1"
     modified_base   4085
     misc_feature    4122..4188
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="msDNA"
     motif           complement(4190..4202)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="primer"
                     /modified_by="katecrawford"
                     /label="a2"
     extention       complement(4204..4218)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="extended primer"
                     /modified_by="katecrawford"
                     /label="extended a2"
     terminator      4242..4600
                     /standard_name="tGal7"
     promoter        complement(4614..4632)
                     /standard_name="T7_promoter"
     misc_feature    4644..4658
                     /standard_name="lacZ_a"
     CDS             4659
                     /codon_start=1
                     /transl_table=1
                     /standard_name="lacZα-Broken"
     CDS             4660..5020
                     /codon_start=1
                     /transl_table=1
                     /translation="TS*LGKPWRYPT*SPCSTSPFRQLA**RRGPHRSPFPTVAQPEW
                     RMARRAL*RRIKRGGCGGYAQRDRYTCQRPSARSFRFLPFLSRHVRRLSPSSSKSGAP
                     FRVPI*CFTAPRPQKT*L"
                     /standard_name="lacZα"
     rep_origin      4803..5258
     CDS             complement(5389..6192)
                     /codon_start=1
                     /transl_table=1
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
                     /standard_name="URA3"
     promoter        complement(6193..6413)
                     /standard_name="URA3 promoter"
     misc_feature    6678..90
                     /standard_name="CEN/ARS"
ORIGIN      
        1 ttttctttaa tttctttttt tactttctat ttttaattta tatatttata ttaaaaaatt
       61 taaattataa ttatttttat agcacgtgat gaaaaggacc caggtggcac ttttcgggga
      121 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      181 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
      241 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
      301 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
      361 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
      421 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
      481 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
      541 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
      601 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
      661 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
      721 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
      781 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
      841 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
      901 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
      961 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     1021 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     1081 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     1141 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     1201 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1261 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     1321 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     1381 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     1441 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     1501 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     1561 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     1621 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     1681 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     1741 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     1801 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     1861 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcaagt agagggggta
     1921 atttttcccc tttattttgt tcatacattc ttaaattgct ttgcctctcc ttttggaaag
     1981 ctatacttcg gagcactgtt gagcgaaggc tcattagata tattttctgt cattttcctt
     2041 aacccaaaaa taagggaaag ggtccaaaaa gcgctcggac aactgttgac cgtgatccga
     2101 aggactggct atacagtgtt cacaaaatag ccaagctgaa aataatgtgt agctatgttc
     2161 agttagtttg gctagcaaag atataaaagc aggtcggaaa tatttatggg cattattatg
     2221 cagagcatca acatgataaa aaaaaacagt tgaatattcc ctcaaaacgc cacatgacaa
     2281 aaacttcaaa gctggatgcg ctgcgggcgg ctactagtag ggaagatttg gcgaagattc
     2341 tcgacataaa gttggtgttt ctgacaaacg tgttgtaccg cataggatcc gacaaccagt
     2401 atacgcaatt cacaataccc aaaaagggta aaggtgtccg caccatcagc gcaccaacgg
     2461 accgacttaa ggatatacag aggaggattt gtgatcttct tagtgactgt agggatgaaa
     2521 tctttgcgat taggaagatc tctaataatt actcattcgg cttcgaaaga ggaaaatcaa
     2581 ttatactcaa tgcttacaag catcgaggga agcaaattat attgaacatc gaccttaagg
     2641 acttctttga gagctttaac tttgggagag tccgggggta ctttctctcc aaccaggact
     2701 tcttgttgaa cccagttgtg gcaacaacgt tggcgaaggc cgcctgctac aacgggactc
     2761 tgcctcaggg gtccccatgt tcccctatta taagtaacct tatctgtaac attatggaca
     2821 tgcggctcgc aaagctcgcc aagaagtacg gctgcactta tagtcgatat gcggatgaca
     2881 ttacgatcag caccaataaa aataccttcc cgttggagat ggcgactgtg cagcctgaag
     2941 gggttgtgct gggcaaagtg ctcgtaaagg agattgaaaa ttcaggtttc gagattaacg
     3001 attctaagac tagattgacc tacaaaacaa gtaggcaaga agtcaccggg ctgacggtta
     3061 atcggattgt aaacattgat cggtgctact acaaaaagac gagggcgctg gctcacgcat
     3121 tgtatcggac aggagaatat aaggtcccag acgagaacgg tgttctggta tctggagggc
     3181 ttgacaagtt ggagggtatg tttgggttta tcgaccaggt ggataaattc aacaacatta
     3241 aaaaaaagtt gaataagcaa cccgacagat atgttctgac aaatgccact ttgcacggat
     3301 ttaagctcaa attgaacgcc agggagaaag cctatagcaa attcatctac tacaaattct
     3361 tccacggtaa tacttgtccc acgatcataa cagagggtaa gacggatagg atttacctta
     3421 aagctgccct ccatagcctc gagacaagtt atcctgaact gtttcgggag aaaacagata
     3481 gtaagaagaa ggagataaat ctgaatattt ttaaaagcaa tgagaagacc aagtatttcc
     3541 tggatctcag cggcggcaca gcagacctca agaaattcgt ggaacgctac aaaaataact
     3601 acgcttccta ttacggcagc gtaccgaaac aaccggtgat aatggtgctt gataacgaca
     3661 caggcccgtc agacctgttg aactttttga gaaacaaagt taagagttgt ccagatgatg
     3721 taacagaaat gcgcaagatg aagtacatac atgtgtttta caatctgtac atagttctga
     3781 ctcccctgtc tccatctgga gagcaaacgt ctatggagga cctctttcct aaagatatat
     3841 tggacattaa gatagatggc aagaaattca ataaaaacaa tgacggtgac tccaaaacag
     3901 agtatgggaa gcacatattc tcaatgcgcg ttgtacgaga taaaaagagg aagatagatt
     3961 tcaaggcatt ttgctgtatc ttcgatgcta ttaaggatat taaagaacat tacaaactga
     4021 tgttgaattc ctaggcggcc gcgggcattt aactatgata agattccgta cacgcatgta
     4081 ggcagatttg ttggttgtga atcgcaacca gtggccttaa tggcaggagg aatcgcctcc
     4141 ctaaaatcct tgattcagag ctatacggca ggtgtgctgt gcgaaggagt gcctgcatgc
     4201 gtgtacggaa tcttatcaac cggttagtaa tgagtttaaa caaagaaagt ggaatattca
     4261 ttcatatcat attttttcta ttaactgcct ggtttctttt aaatttttta ttggttgtcg
     4321 acttgaacgg agtgacaata tatatatata tatatttaat aatgacatca ttatctgtaa
     4381 atctgattct taatgctatt ctagttatgt aagagtggtc ctttccataa aaaaaaaaaa
     4441 aaagaaaaaa gaattttagg aatacaatgc agcttgtaag taaaatctgg aatattcata
     4501 tcgccacaac ttcttatgct tataaaagca ctaatgcctg aatttatgtt gaaaatatgt
     4561 gtcacaaata aagaaactgt gacatctgac acatttccac gtacccaatt cgccctatag
     4621 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     4681 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     4741 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     4801 cgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4861 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     4921 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     4981 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     5041 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     5101 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     5161 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     5221 taacgcgaat tttaacaaaa tattaacgtt tacaatttcc tgatgcggta ttttctcctt
     5281 acgcatctgt gcggtatttc acaccgcata gggtaataac tgatataatt aaattgaagc
     5341 tctaatttgt gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg
     5401 gccgcatctt ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt
     5461 agcatccctt ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga
     5521 gaccacatca tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc
     5581 cacaccgggt gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg
     5641 agcaataaag ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt
     5701 atattctcca gtagataggg agcccttgca tgacaattct gctaacatca aaaggcctct
     5761 aggttccttt gttacttctt ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac
     5821 cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta
     5881 ctgcaatttg actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt
     5941 gtacttggcg gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa
     6001 gatatccaca tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag
     6061 taattccttg gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat
     6121 gatattaaat agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt
     6181 agctttcgac atgatttatc ttcgtttcct gcaggttttt gttctgtgca gttgggttaa
     6241 gaatactggg caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag
     6301 tctgtgctcc ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca
     6361 aagaaaccga aatcaaaaaa aagaataaaa aaaaaatgat gaattgaatt gaaaagctgt
     6421 ggtatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     6481 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     6541 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     6601 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     6661 aatggtttct taggacggat cgcttgcctg taacttacac gcgcctcgta tcttttaatg
     6721 atggaataat ttgggaattt actctgtgtt tatttatttt tatgttttgt atttggattt
     6781 tagaaagtaa ataaagaagg tagaagagtt acggaatgaa gaaaaaaaaa taaacaaagg
     6841 tttaaaaaat ttcaacaaaa agcgtacttt acatatatat ttattagaca agaaaagcag
     6901 attaaataga tatacattcg attaacgata agtaaaatgt aaaatcacag gattttcgtg
     6961 tgtggtcttc tacacagaca agatgaaaca attcggcatt aatacctgag agcaggaaga
     7021 gcaagataaa aggtagtatt tgttggcgat ccccctagag tcttttacat cttcggaaaa
     7081 caaaaactat t
//
