LOCUS       pSCL006                 5466 bp    DNA     circular UNA 19-JAN-2022
DEFINITION  .
ACCESSION   urn.local...309-c3pkuaa
VERSION     urn.local...309-c3pkuaa
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##SnapGene_Notes-START##                                           
            Confirmed Experimentally :: 0                                      
            Transformed Into         :: unspecified                            
            Sequence Document Type   :: UNA                                    
            SnapGene UUID            :: 04c4275c-0140-4756-8b54-292289f5664a   
            Sequence Type            :: Synthetic                              
            ##SnapGene_Notes-END##                                             
            ##Imported_From-START##                                            
            Path     :: /Users/slopez/Dropbox                                  
                        (Gladstone)/Santi_L_docs/shipman/projects/retrons/crisp
                        ey_maps                                                
            Filename :: pSCL_006.dna                                           
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     misc_feature    84..316
                     /standard_name="pGal10"
     misc_feature    317..1303
                     /standard_name="ec86 RT sc-optimized"
     misc_feature    1304..1528
                     /standard_name="Gal10 terminator"
     misc_feature    1537..1555
                     /standard_name="t7 promoter"
     misc_feature    1743..2049
                     /standard_name="f1 ori"
     misc_feature    2444..3100
                     /standard_name="His3"
     misc_feature    3101..3417
                     /standard_name="pHis3"
     misc_feature    3699..3803
                     /standard_name="Amp promoter"
     misc_feature    3804..4664
                     /standard_name="Amp"
     misc_feature    4835..5423
                     /standard_name="ori"
ORIGIN      
        1 aggcacatct gcgtttcagg aacgcgaccg gtgaagacga ggacgcacgg aggagagtct
       61 tccgtcggag ggctgtcgcc cgctcggcgg cttctaatcc gtacttcaat atagcaatga
      121 gcagttaagc gtattactga aagttccaaa gagaaggttt ttttaggcta agataatggg
      181 gctctttaca tttccacaac atataagtaa gattagatat ggatatgtat atggtggtaa
      241 tgccatgtaa tatgattatt aaacttcttt gcgtccatcc aaaaaaaaag taagaatttt
      301 tgaaaattca atataaatgc caccaaagaa gaaaagaaag gtcaagtccg ctgaatactt
      361 gaacaccttc agattgagaa atttgggttt gccagtcatg aacaacttgc acgatatgtc
      421 taaggctacc agaatttctg ttgaaacctt gagattattg atctacaccg ccgatttcag
      481 atacagaatc tacactgttg aaaagaaggg tccagaaaag agaatgagaa ctatctacca
      541 accatccaga gaattgaagg ctttacaagg ttgggttttg agaaacatct tggacaagtt
      601 atcctcctcc ccattttcta tcggttttga aaagcaccaa tccatcttga acaatgccac
      661 tccacatatt ggtgccaact tcattttgaa catcgacttg gaagatttct tcccatcttt
      721 gactgctaac aaggttttcg gtgttttcca ttctttgggt tacaacagat tgatctcctc
      781 tgttttgacc aagatttgct gctacaagaa cttgttgcca caaggtgctc catcttctcc
      841 aaaattggct aacttgattt gctccaagtt ggactacaga attcaaggtt atgctggttc
      901 tagaggtttg atatacacta gatacgctga tgacttgacc ttgtctgctc aatctatgaa
      961 gaaagttgtt aaggccagag acttcttgtt ctccattatt ccatcagaag gtttggtcat
     1021 caactccaaa aagacctgta tttctggtcc aagatcccaa agaaaagtta ccggtttggt
     1081 tatctcccaa gaaaaggttg gtattggtag agaaaagtac aaagaaatca gagccaagat
     1141 ccaccatatt ttctgcggta aatcctccga aatcgaacat gttagaggtt ggttgtcttt
     1201 catcttgtcc gttgattcta agtcccacag aagattgatt acctacatct ctaagttgga
     1261 aaagaagtac ggtaagaacc cattgaacaa ggctaagact tgatttgcca gcttactatc
     1321 cttcttgaaa atatgcactc tatatctttt agttcttaat tgcaacacat agatttgctg
     1381 tataacgaat tttatgctat tttttaaatt tggagttcag tgataaaagt gtcacagcga
     1441 atttcctcac atgtagggac cgaattgttt acaagttctc tgtaccacca tggagacatc
     1501 aaaaattgaa aatctatgga aagatatgca attcgcccta tagtgagtcg tattacgcgc
     1561 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     1621 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     1681 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcgacgcg ccctgtagcg
     1741 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     1801 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     1861 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     1921 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     1981 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     2041 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     2101 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     2161 aaatattaac gtttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     2221 ttcacaccgc atagatccgt cgagttcaag agaaaaaaaa agaaaaagca aaaagaaaaa
     2281 aggaaagcgc gcctcgttca gaatgacacg tatagaatga tgcattacct tgtcatcttc
     2341 agtatcatac tgttcgtata catacttact gacattcata ggtatacata tatacacatg
     2401 tatatatatc gtatgctgca gctttaaata atcggtgtca ctacataaga acacctttgg
     2461 tggagggaac atcgttggta ccattgggcg aggtggcttc tcttatggca accgcaagag
     2521 ccttgaacgc actctcacta cggtgatgat cattcttgcc tcgcagacaa tcaacgtgga
     2581 gggtaattct gctagcctct gcaaagcttt caagaaaatg cgggatcatc tcgcaagaga
     2641 gatctcctac tttctccctt tgcaaaccaa gttcgacaac tgcgtacggc ctgttcgaaa
     2701 gatctaccac cgctctggaa agtgcctcat ccaaaggcgc aaatcctgat ccaaaccttt
     2761 ttactccacg cgccagtagg gcctctttaa aagcttgacc gagagcaatc ccgcagtctt
     2821 cagtggtgtg atggtcgtct atgtgtaagt caccaatgca ctcaacgatt agcgaccagc
     2881 cggaatgctt ggccagagca tgtatcatat ggtccagaaa ccctatacct gtgtggacgt
     2941 taatcacttg cgattgtgtg gcctgttctg ctactgcttc tgcctctttt tctgggaaga
     3001 tcgagtgctc tatcgctagg ggaccaccct ttaaagagat cgcaatctga atcttggttt
     3061 catttgtaat acgctttact agggctttct gctctgtcat ctttgccttc gtttatcttg
     3121 cctgctcatt ttttagtata ttcttcgaag aaatcacatt actttatata atgtataatt
     3181 cattatgtga taatgccaat cgctaagaaa aaaaaagagt catccgctag gggaaaaaaa
     3241 aaaatgaaaa tcattaccga ggcataaaaa aatatagagt gtactagagg aggccaagag
     3301 taatagaaaa agaaaattgc gggaaaggac tgtgttatga cttccctgac taatgccgtg
     3361 ttcaaacgat acctggcagt gactcctagc gctcaccaag ctcttaaaac gggaatttat
     3421 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     3481 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     3541 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     3601 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     3661 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     3721 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     3781 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     3841 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     3901 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     3961 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     4021 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     4081 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     4141 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     4201 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     4261 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     4321 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     4381 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     4441 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     4501 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     4561 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     4621 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     4681 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     4741 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     4801 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     4861 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     4921 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     4981 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5041 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5101 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5161 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5221 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5281 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5341 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5401 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5461 tttgct
//
