LOCUS       pSCL039                 5602 bp    DNA     circular UNA 08-APR-2022
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...16-d4vdn2g
VERSION     urn.local...16-d4vdn2g
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    join(5189..5602,1..90)
                     /standard_name="CEN/ARS"
     promoter        127..231
                     /standard_name="AmpR promoter"
     CDS             232..300
                     /standard_name="AmpR"
     CDS             301..1092
                     /standard_name="AmpR"
     rep_origin      1263..1851
     promoter        1907..2267
                     /standard_name="pGal7"
     misc_feature    2268..2314
                     /standard_name="HH-Ribozyme"
     extention       2316..2330
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="extended primer"
     extention       2331..2342
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="primer"
     misc_feature    <2331..2411
                     /standard_name="msr"
     misc_RNA        complement(2401..2544)
                     /created_by="katecrawford"
                     /label="msDNA"
     CDS             2424..2523
                     /standard_name="Ade2 donor"
     extention       complement(2546..2557)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="primer"
     extention       complement(2558..2572)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="extended primer"
     misc_difference 2573..2590
                     /standard_name="gRNA (18 bases)"
     misc_feature    2591..2681
                     /standard_name="modified SpCas9 scaffold"
     misc_feature    2682..2752
                     /standard_name="HDV-Ribozyme"
     terminator      2753..3111
                     /standard_name="tGal7"
     promoter        complement(3125..3143)
                     /standard_name="T7_promoter"
     misc_feature    3155..3169
                     /standard_name="lacZ_a"
     CDS             3170
                     /standard_name="lacZα-Broken"
     CDS             3171..3531
                     /standard_name="lacZα"
     rep_origin      3314..3769
     CDS             complement(3900..4703)
                     /standard_name="URA3"
     promoter        complement(4704..4924)
                     /standard_name="URA3 promoter"
ORIGIN      
        1 ttttctttaa tttctttttt tactttctat ttttaattta tatatttata ttaaaaaatt
       61 taaattataa ttatttttat agcacgtgat gaaaaggacc caggtggcac ttttcgggga
      121 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      181 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
      241 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
      301 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
      361 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
      421 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
      481 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
      541 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
      601 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
      661 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
      721 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
      781 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
      841 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
      901 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
      961 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     1021 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     1081 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     1141 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     1201 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1261 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     1321 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     1381 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     1441 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     1501 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     1561 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     1621 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     1681 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     1741 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     1801 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     1861 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcaagt agagggggta
     1921 atttttcccc tttattttgt tcatacattc ttaaattgct ttgcctctcc ttttggaaag
     1981 ctatacttcg gagcactgtt gagcgaaggc tcattagata tattttctgt cattttcctt
     2041 aacccaaaaa taagggaaag ggtccaaaaa gcgctcggac aactgttgac cgtgatccga
     2101 aggactggct atacagtgtt cacaaaatag ccaagctgaa aataatgtgt agctatgttc
     2161 agttagtttg gctagcaaag atataaaagc aggtcggaaa tatttatggg cattattatg
     2221 cagagcatca acatgataaa aaaaaacagt tgaatattcc ctcaaaaggg tgcgcatctg
     2281 atgagtccgt gaggacgaaa cgagctagct cgtcatgata agattccgta tgcgcaccct
     2341 tagcgagagg tttatcatta aggtcaacct ctggatgttg tttcggcatc ctgcattgaa
     2401 tctgagttac tgtctgtttt ccttggaaat gttctattta gaaacagggg aattgcttat
     2461 taacgaaatt gcctgaaggc ctcacaactc tggacattat accattgatg cttgcgtcac
     2521 ttcaggaaac ccgtttcttc tgacgtaagg gtgcgcatac ggaatcttat caattaacga
     2581 aattgcccca gtttcagagc tatgctggaa acagcatagc aagttgaaat aaggctagtc
     2641 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tgatggccgg catggtccca
     2701 gcctcctcgc tggcgccggc tgggcaacac cttcgggtgg cgaatgggac ttaaagaaag
     2761 tggaatattc attcatatca tattttttct attaactgcc tggtttcttt taaatttttt
     2821 attggttgtc gacttgaacg gagtgacaat atatatatat atatatttaa taatgacatc
     2881 attatctgta aatctgattc ttaatgctat tctagttatg taagagtggt cctttccata
     2941 aaaaaaaaaa aaaagaaaaa agaattttag gaatacaatg cagcttgtaa gtaaaatctg
     3001 gaatattcat atcgccacaa cttcttatgc ttataaaagc actaatgcct gaatttatgt
     3061 tgaaaatatg tgtcacaaat aaagaaactg tgacatctga cacatttcca cgtacccaat
     3121 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     3181 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     3241 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     3301 ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     3361 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     3421 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     3481 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     3541 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     3601 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     3661 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     3721 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc ctgatgcggt
     3781 attttctcct tacgcatctg tgcggtattt cacaccgcat agggtaataa ctgatataat
     3841 taaattgaag ctctaatttg tgagtttagt atacatgcat ttacttataa tacagttttt
     3901 tagttttgct ggccgcatct tctcaaatat gcttcccagc ctgcttttct gtaacgttca
     3961 ccctctacct tagcatccct tccctttgca aatagtcctc ttccaacaat aataatgtca
     4021 gatcctgtag agaccacatc atccacggtt ctatactgtt gacccaatgc gtctcccttg
     4081 tcatctaaac ccacaccggg tgtcataatc aaccaatcgt aaccttcatc tcttccaccc
     4141 atgtctcttt gagcaataaa gccgataaca aaatctttgt cgctcttcgc aatgtcaaca
     4201 gtacccttag tatattctcc agtagatagg gagcccttgc atgacaattc tgctaacatc
     4261 aaaaggcctc taggttcctt tgttacttct tctgccgcct gcttcaaacc gctaacaata
     4321 cctgggccca ccacaccgtg tgcattcgta atgtctgccc attctgctat tctgtataca
     4381 cccgcagagt actgcaattt gactgtatta ccaatgtcag caaattttct gtcttcgaag
     4441 agtaaaaaat tgtacttggc ggataatgcc tttagcggct taactgtgcc ctccatggaa
     4501 aaatcagtca agatatccac atgtgttttt agtaaacaaa ttttgggacc taatgcttca
     4561 actaactcca gtaattcctt ggtggtacga acatccaatg aagcacacaa gtttgtttgc
     4621 ttttcgtgca tgatattaaa tagcttggca gcaacaggac taggatgagt agcagcacgt
     4681 tccttatatg tagctttcga catgatttat cttcgtttcc tgcaggtttt tgttctgtgc
     4741 agttgggtta agaatactgg gcaatttcat gtttcttcaa cactacatat gcgtatatat
     4801 accaatctaa gtctgtgctc cttccttcgt tcttccttct gttcggagat taccgaatca
     4861 aaaaaatttc aaagaaaccg aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaat
     4921 tgaaaagctg tggtatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     4981 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     5041 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     5101 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     5161 gtcatgataa taatggtttc ttaggacgga tcgcttgcct gtaacttaca cgcgcctcgt
     5221 atcttttaat gatggaataa tttgggaatt tactctgtgt ttatttattt ttatgttttg
     5281 tatttggatt ttagaaagta aataaagaag gtagaagagt tacggaatga agaaaaaaaa
     5341 ataaacaaag gtttaaaaaa tttcaacaaa aagcgtactt tacatatata tttattagac
     5401 aagaaaagca gattaaatag atatacattc gattaacgat aagtaaaatg taaaatcaca
     5461 ggattttcgt gtgtggtctt ctacacagac aagatgaaac aattcggcat taatacctga
     5521 gagcaggaag agcaagataa aaggtagtat ttgttggcga tccccctaga gtcttttaca
     5581 tcttcggaaa acaaaaacta tt
//
