LOCUS       pSCL110                 5572 bp    DNA     circular UNA 08-APR-2022
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...gc-ddqau4m
VERSION     urn.local...gc-ddqau4m
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    join(5159..5572,1..90)
                     /standard_name="CEN/ARS"
     promoter        127..231
                     /standard_name="AmpR promoter"
     CDS             232..300
                     /standard_name="AmpR"
     CDS             301..1092
                     /standard_name="AmpR"
     rep_origin      1263..1851
     promoter        1907..2267
                     /standard_name="pGal7"
     misc_feature    2268..2314
                     /standard_name="HH-Ribozyme"
     misc_feature    2315..2396
                     /standard_name="msr"
     misc_feature    2316..2327
                     /standard_name="inverted repeat"
                     /modified_by="katecrawford"
                     /label="a1"
     misc_RNA        complement(2386..2529)
                     /created_by="katecrawford"
                     /label="msDNA"
     donor           2409..2508
                     /standard_name="donor"
     grna            2444..2461
                     /standard_name="trp2 gRNA"
     mutation        2464..2466
                     /standard_name="GAA -> TAA"
     misc_feature    complement(2531..2542)
                     /standard_name="inverted repeat"
                     /modified_by="katecrawford"
                     /label="a2"
     grna            2543..2560
                     /standard_name="trp2 gRNA"
     misc_feature    2561..2651
                     /standard_name="modified SpCas9 scaffold"
     misc_feature    2652..2722
                     /standard_name="HDV-Ribozyme"
     terminator      2723..3081
                     /standard_name="tGal7"
     promoter        complement(3095..3113)
                     /standard_name="T7_promoter"
     misc_feature    3125..3139
                     /standard_name="lacZ_a"
     CDS             3140
                     /standard_name="lacZα-Broken"
     CDS             3141..3501
                     /standard_name="lacZα"
     rep_origin      3284..3739
     CDS             complement(3870..4673)
                     /standard_name="URA3"
     promoter        complement(4674..4894)
                     /standard_name="URA3 promoter"
ORIGIN      
        1 ttttctttaa tttctttttt tactttctat ttttaattta tatatttata ttaaaaaatt
       61 taaattataa ttatttttat agcacgtgat gaaaaggacc caggtggcac ttttcgggga
      121 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      181 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
      241 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
      301 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
      361 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
      421 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
      481 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
      541 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
      601 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
      661 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
      721 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
      781 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
      841 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
      901 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
      961 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     1021 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     1081 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     1141 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     1201 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1261 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     1321 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     1381 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     1441 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     1501 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     1561 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     1621 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     1681 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     1741 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     1801 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     1861 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcaagt agagggggta
     1921 atttttcccc tttattttgt tcatacattc ttaaattgct ttgcctctcc ttttggaaag
     1981 ctatacttcg gagcactgtt gagcgaaggc tcattagata tattttctgt cattttcctt
     2041 aacccaaaaa taagggaaag ggtccaaaaa gcgctcggac aactgttgac cgtgatccga
     2101 aggactggct atacagtgtt cacaaaatag ccaagctgaa aataatgtgt agctatgttc
     2161 agttagtttg gctagcaaag atataaaagc aggtcggaaa tatttatggg cattattatg
     2221 cagagcatca acatgataaa aaaaaacagt tgaatattcc ctcaaaaggg tgcgcatctg
     2281 atgagtccgt gaggacgaaa cgagctagct cgtcatgcgc acccttagcg agaggtttat
     2341 cattaaggtc aacctctgga tgttgtttcg gcatcctgca ttgaatctga gttactgtct
     2401 gttttcctat atctaaaatt ggcacaattg aacaaccctg atagaaagga atcatttctg
     2461 ttgtaaagtg ctaagacaaa taatgaatta gatcgttatt cattcataag gaaacccgtt
     2521 tcttctgacg taagggtgcg cagaaaggaa tcatttctgt gtttcagagc tatgctggaa
     2581 acagcatagc aagttgaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     2641 cggtgctttt tgatggccgg catggtccca gcctcctcgc tggcgccggc tgggcaacac
     2701 cttcgggtgg cgaatgggac ttaaagaaag tggaatattc attcatatca tattttttct
     2761 attaactgcc tggtttcttt taaatttttt attggttgtc gacttgaacg gagtgacaat
     2821 atatatatat atatatttaa taatgacatc attatctgta aatctgattc ttaatgctat
     2881 tctagttatg taagagtggt cctttccata aaaaaaaaaa aaaagaaaaa agaattttag
     2941 gaatacaatg cagcttgtaa gtaaaatctg gaatattcat atcgccacaa cttcttatgc
     3001 ttataaaagc actaatgcct gaatttatgt tgaaaatatg tgtcacaaat aaagaaactg
     3061 tgacatctga cacatttcca cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     3121 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     3181 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     3241 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc
     3301 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     3361 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     3421 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     3481 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     3541 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     3601 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     3661 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     3721 atattaacgt ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     3781 cacaccgcat agggtaataa ctgatataat taaattgaag ctctaatttg tgagtttagt
     3841 atacatgcat ttacttataa tacagttttt tagttttgct ggccgcatct tctcaaatat
     3901 gcttcccagc ctgcttttct gtaacgttca ccctctacct tagcatccct tccctttgca
     3961 aatagtcctc ttccaacaat aataatgtca gatcctgtag agaccacatc atccacggtt
     4021 ctatactgtt gacccaatgc gtctcccttg tcatctaaac ccacaccggg tgtcataatc
     4081 aaccaatcgt aaccttcatc tcttccaccc atgtctcttt gagcaataaa gccgataaca
     4141 aaatctttgt cgctcttcgc aatgtcaaca gtacccttag tatattctcc agtagatagg
     4201 gagcccttgc atgacaattc tgctaacatc aaaaggcctc taggttcctt tgttacttct
     4261 tctgccgcct gcttcaaacc gctaacaata cctgggccca ccacaccgtg tgcattcgta
     4321 atgtctgccc attctgctat tctgtataca cccgcagagt actgcaattt gactgtatta
     4381 ccaatgtcag caaattttct gtcttcgaag agtaaaaaat tgtacttggc ggataatgcc
     4441 tttagcggct taactgtgcc ctccatggaa aaatcagtca agatatccac atgtgttttt
     4501 agtaaacaaa ttttgggacc taatgcttca actaactcca gtaattcctt ggtggtacga
     4561 acatccaatg aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa tagcttggca
     4621 gcaacaggac taggatgagt agcagcacgt tccttatatg tagctttcga catgatttat
     4681 cttcgtttcc tgcaggtttt tgttctgtgc agttgggtta agaatactgg gcaatttcat
     4741 gtttcttcaa cactacatat gcgtatatat accaatctaa gtctgtgctc cttccttcgt
     4801 tcttccttct gttcggagat taccgaatca aaaaaatttc aaagaaaccg aaatcaaaaa
     4861 aaagaataaa aaaaaaatga tgaattgaat tgaaaagctg tggtatggtg cactctcagt
     4921 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     4981 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     5041 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     5101 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttaggacgga
     5161 tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa tttgggaatt
     5221 tactctgtgt ttatttattt ttatgttttg tatttggatt ttagaaagta aataaagaag
     5281 gtagaagagt tacggaatga agaaaaaaaa ataaacaaag gtttaaaaaa tttcaacaaa
     5341 aagcgtactt tacatatata tttattagac aagaaaagca gattaaatag atatacattc
     5401 gattaacgat aagtaaaatg taaaatcaca ggattttcgt gtgtggtctt ctacacagac
     5461 aagatgaaac aattcggcat taatacctga gagcaggaag agcaagataa aaggtagtat
     5521 ttgttggcga tccccctaga gtcttttaca tcttcggaaa acaaaaacta tt
//
