LOCUS       pSCL102                10404 bp    DNA     circular UNA 08-APR-2022
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...106-dc0nf00
VERSION     urn.local...106-dc0nf00
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        39..271
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Gal10p"
     promoter        272..702
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Gal1/10 promoter"
     CDS             <728..>1711
                     /standard_name="RT Ec86 optimized"
     misc_feature    731..754
                     /standard_name="SV40 NLS"
     p2a             1712..1768
                     /standard_name="P2A"
     CDS             <1769..>5869
                     /codon_start=1
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Cas9"
     nls             5870..5902
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40"
     misc_feature    5917..6163
                     /standard_name="CYC1 terminator"
     misc_feature_   complement(6633..7221)
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="colE1_high_copy"
     gene            complement(7392..8252)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AmpR"
     promoter        8253..8357
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AmpR promoter"
     promoter        8769..8955
                     /gene="S. cerevisiae HIS3"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="HIS3 promoter"
     CDS             8956..9615
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase,
                     required for histidine biosynthesis"
                     /note="yeast auxotrophic marker"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="HIS3"
     rep_origin      9875..10330
ORIGIN      
        1 tcagcggact tgacctttct tttcttcttt ggtggcattt atattgaatt ttcaaaaatt
       61 cttacttttt ttttggatgg acgcaaagaa gtttaataat catattacat ggcattacca
      121 ccatatacat atccatatct aatcttactt atatgttgtg gaaatgtaaa gagccccatt
      181 atcttagcct aaaaaaacct tctctttgga actttcagta atacgcttaa ctgctcattg
      241 ctatattgaa gtacggatta gaagccgccg agcgggcgac agccctccga cggaagactc
      301 tcctccgtgc gtcctcgtct tcaccggtcg cgttcctgaa acgcagatgt gcctcgcgcc
      361 gcactgctcc gaacaataaa gattctacaa tactagcttt tatggttatg aagaggaaaa
      421 attggcagta acctggcccc acaaaccttc aaattaacga atcaaattaa caaccatagg
      481 atgataatgc gattagtttt ttagccttat ttctggggta attaatcagc gaagcgatga
      541 tttttgatct attaacagat atataaatgg aaaagctgca taaccacttt aactaatact
      601 ttcaacattt tcagtttgta ttacttctta ttcaaatgtc ataaaagtat caacaaaaaa
      661 ttgttaatat acctctatac tttaacgtca aggagaaaaa acaactagtg gatcccccgg
      721 gaaaaaaatg ccaccaaaga agaaaagaaa ggtcaagtcc gctgaatact tgaacacctt
      781 cagattgaga aatttgggtt tgccagtcat gaacaacttg cacgatatgt ctaaggctac
      841 cagaatttct gttgaaacct tgagattatt gatctacacc gccgatttca gatacagaat
      901 ctacactgtt gaaaagaagg gtccagaaaa gagaatgaga actatctacc aaccatccag
      961 agaattgaag gctttacaag gttgggtttt gagaaacatc ttggacaagt tatcctcctc
     1021 cccattttct atcggttttg aaaagcacca atccatcttg aacaatgcca ctccacatat
     1081 tggtgccaac ttcattttga acatcgactt ggaagatttc ttcccatctt tgactgctaa
     1141 caaggttttc ggtgttttcc attctttggg ttacaacaga ttgatctcct ctgttttgac
     1201 caagatttgc tgctacaaga acttgttgcc acaaggtgct ccatcttctc caaaattggc
     1261 taacttgatt tgctccaagt tggactacag aattcaaggt tatgctggtt ctagaggttt
     1321 gatatacact agatacgctg atgacttgac cttgtctgct caatctatga agaaagttgt
     1381 taaggccaga gacttcttgt tctccattat tccatcagaa ggtttggtca tcaactccaa
     1441 aaagacctgt atttctggtc caagatccca aagaaaagtt accggtttgg ttatctccca
     1501 agaaaaggtt ggtattggta gagaaaagta caaagaaatc agagccaaga tccaccatat
     1561 tttctgcggt aaatcctccg aaatcgaaca tgttagaggt tggttgtctt tcatcttgtc
     1621 cgttgattct aagtcccaca gaagattgat tacctacatc tctaagttgg aaaagaagta
     1681 cggtaagaac ccattgaaca aggctaagac tgcaacaaac ttctctctgc tgaaacaagc
     1741 cggagatgtc gaagagaatc ctggaccgga caagaagtac tccattgggc tcgatatcgg
     1801 cacaaacagc gtcggctggg ccgtcattac ggacgagtac aaggtgccga gcaaaaaatt
     1861 caaagttctg ggcaataccg atcgccacag cataaagaag aacctcattg gcgccctcct
     1921 gttcgactcc ggggagacgg ccgaagccac gcggctcaaa agaacagcac ggcgcagata
     1981 tacccgcaga aagaatcgga tctgctacct gcaggagatc tttagtaatg agatggctaa
     2041 ggtggatgac tctttcttcc ataggctgga ggagtccttt ttggtggagg aggataaaaa
     2101 gcacgagcgc cacccaatct ttggcaatat cgtggacgag gtggcgtacc atgaaaagta
     2161 cccaaccata tatcatctga ggaagaagct tgtagacagt actgataagg ctgacttgcg
     2221 gttgatctat ctcgcgctgg cgcatatgat caaatttcgg ggacacttcc tcatcgaggg
     2281 ggacctgaac ccagacaaca gcgatgtcga caaactcttt atccaactgg ttcagactta
     2341 caatcagctt ttcgaagaga acccgatcaa cgcatccgga gttgacgcca aagcaatcct
     2401 gagcgctagg ctgtccaaat cccggcggct cgaaaacctc atcgcacagc tccctgggga
     2461 gaagaagaac ggcctgtttg gtaatcttat cgccctgtca ctcgggctga cccccaactt
     2521 taaatctaac ttcgacctgg ccgaagatgc caagcttcaa ctgagcaaag acacctacga
     2581 tgatgatctc gacaatctgc tggcccagat cggcgaccag tacgcagacc tttttttggc
     2641 ggcaaagaac ctgtcagacg ccattctgct gagtgatatt ctgcgagtga acacggagat
     2701 caccaaagct ccgctgagcg ctagtatgat caagcgctat gatgagcacc accaagactt
     2761 gactttgctg aaggcccttg tcagacagca actgcctgag aagtacaagg aaattttctt
     2821 cgatcagtct aaaaatggct acgccggata cattgacggc ggagcaagcc aggaggaatt
     2881 ttacaaattt attaagccca tcttggaaaa aatggacggc accgaggagc tgctggtaaa
     2941 gcttaacaga gaagatctgt tgcgcaaaca gcgcactttc gacaatggaa gcatccccca
     3001 ccagattcac ctgggcgaac tgcacgctat cctcaggcgg caagaggatt tctacccctt
     3061 tttgaaagat aacagggaaa agattgagaa aatcctcaca tttcggatac cctactatgt
     3121 aggccccctc gcccggggaa attccagatt cgcgtggatg actcgcaaat cagaagagac
     3181 catcactccc tggaacttcg aggaagtcgt ggataagggg gcctctgccc agtccttcat
     3241 cgaaaggatg actaactttg ataaaaatct gcctaacgaa aaggtgcttc ctaaacactc
     3301 tctgctgtac gagtacttca cagtttataa cgagctcacc aaggtcaaat acgtcacaga
     3361 agggatgaga aagccagcat tcctgtctgg agagcagaag aaagctatcg tggacctcct
     3421 cttcaagacg aaccggaaag ttaccgtgaa acagctcaaa gaagactatt tcaaaaagat
     3481 tgaatgtttc gactctgttg aaatcagcgg agtggaggat cgcttcaacg catccctggg
     3541 aacgtatcac gatctcctga aaatcattaa agacaaggac ttcctggaca atgaggagaa
     3601 cgaggacatt cttgaggaca ttgtcctcac ccttacgttg tttgaagata gggagatgat
     3661 tgaagaacgc ttgaaaactt acgctcatct cttcgacgac aaagtcatga aacagctcaa
     3721 gaggcgccga tatacaggat gggggcggct gtcaagaaaa ctgatcaatg ggatccgaga
     3781 caagcagagt ggaaagacaa tcctggattt tcttaagtcc gatggatttg ccaaccggaa
     3841 cttcatgcag ttgatccatg atgactctct cacctttaag gaggacatcc agaaagcaca
     3901 agtttctggc cagggggaca gtcttcacga gcacatcgct aatcttgcag gtagcccagc
     3961 tatcaaaaag ggaatactgc agaccgttaa ggtcgtggat gaactcgtca aagtaatggg
     4021 aaggcataag cccgagaata tcgttatcga gatggcccga gagaaccaaa ctacccagaa
     4081 gggacagaag aacagtaggg aaaggatgaa gaggattgaa gagggtataa aagaactggg
     4141 gtcccaaatc cttaaggaac acccagttga aaacacccag cttcagaatg agaagctcta
     4201 cctgtactac ctgcagaacg gcagggacat gtacgtggat caggaactgg acatcaatcg
     4261 gctctccgac tacgacgtgg atcatatcgt gccccagtct tttctcaaag atgattctat
     4321 tgataataaa gtgttgacaa gatccgataa aaatagaggg aagagtgata acgtcccctc
     4381 agaagaagtt gtcaagaaaa tgaaaaatta ttggcggcag ctgctgaacg ccaaactgat
     4441 cacacaacgg aagttcgata atctgactaa ggctgaacga ggtggcctgt ctgagttgga
     4501 taaagccggc ttcatcaaaa ggcagcttgt tgagacacgc cagatcacca agcacgtggc
     4561 ccaaattctc gattcacgca tgaacaccaa gtacgatgaa aatgacaaac tgattcgaga
     4621 ggtgaaagtt attactctga agtctaagct ggtctcagat ttcagaaagg actttcagtt
     4681 ttataaggtg agagagatca acaattacca ccatgcgcat gatgcctacc tgaatgcagt
     4741 ggtaggcact gcacttatca aaaaatatcc caagcttgaa tctgaatttg tttacggaga
     4801 ctataaagtg tacgatgtta ggaaaatgat cgcaaagtct gagcaggaaa taggcaaggc
     4861 caccgctaag tacttctttt acagcaatat tatgaatttt ttcaagaccg agattacact
     4921 ggccaatgga gagattcgga agcgaccact tatcgaaaca aacggagaaa caggagaaat
     4981 cgtgtgggac aagggtaggg atttcgcgac agtccggaag gtcctgtcca tgccgcaggt
     5041 gaacatcgtt aaaaagaccg aagtacagac cggaggcttc tccaaggaaa gtatcctccc
     5101 gaaaaggaac agcgacaagc tgatcgcacg caaaaaagat tgggacccca agaaatacgg
     5161 cggattcgat tctcctacag tcgcttacag tgtactggtt gtggccaaag tggagaaagg
     5221 gaagtctaaa aaactcaaaa gcgtcaagga actgctgggc atcacaatca tggagcgatc
     5281 aagcttcgaa aaaaacccca tcgactttct cgaggcgaaa ggatataaag aggtcaaaaa
     5341 agacctcatc attaagcttc ccaagtactc tctctttgag cttgaaaacg gccggaaacg
     5401 aatgctcgct agtgcgggcg agctgcagaa aggtaacgag ctggcactgc cctctaaata
     5461 cgttaatttc ttgtatctgg ccagccacta tgaaaagctc aaagggtctc ccgaagataa
     5521 tgagcagaag cagctgttcg tggaacaaca caaacactac cttgatgaga tcatcgagca
     5581 aataagcgaa ttctccaaaa gagtgatcct cgccgacgct aacctcgata aggtgctttc
     5641 tgcttacaat aagcacaggg ataagcccat cagggagcag gcagaaaaca ttatccactt
     5701 gtttactctg accaacttgg gcgcgcctgc agccttcaag tacttcgaca ccaccataga
     5761 cagaaagcgg tacacctcta caaaggaggt cctggacgcc acactgattc atcagtcaat
     5821 tacggggctc tatgaaacaa gaatcgacct ctctcagctc ggtggagaca gcagggctga
     5881 ccccaagaag aagaggaagg tgtgatctct tctcgagtca tgtaattagt tatgtcacgc
     5941 ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct
     6001 gaagtctagg tccctattta tttttttata gttatgttag tattaagaac gttatttata
     6061 tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa
     6121 accttgcttg agaaggtttt gggacgctcg aaggctttaa tttcaccgcg gtggagctcc
     6181 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
     6241 tttcctgtgt gaaattgtta tccgctcaca attccacaca acataggagc cggaagcata
     6301 aagtgtaaag cctggggtgc ctaatgagtg aggtaactca cattaattgc gttgcgctca
     6361 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6421 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     6481 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     6541 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     6601 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6661 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6721 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6781 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     6841 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     6901 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     6961 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7021 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     7081 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7141 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7201 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     7261 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     7321 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7381 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7441 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     7501 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     7561 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     7621 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     7681 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     7741 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     7801 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     7861 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     7921 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     7981 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     8041 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     8101 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     8161 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     8221 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     8281 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     8341 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     8401 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     8461 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     8521 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     8581 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccataaa
     8641 ttcccgtttt aagagcttgg tgagcgctag gagtcactgc caggtatcgt ttgaacacgg
     8701 cattagtcag ggaagtcata acacagtcct ttcccgcaat tttctttttc tattactctt
     8761 ggcctcctct agtacactct atattttttt atgcctcggt aatgattttc attttttttt
     8821 ttcccctagc ggatgactct ttttttttct tagcgattgg cattatcaca taatgaatta
     8881 tacattatat aaagtaatgt gatttcttcg aagaatatac taaaaaatga gcaggcaaga
     8941 taaacgaagg caaagatgac agagcagaaa gccctagtaa agcgtattac aaatgaaacc
     9001 aagattcaga ttgcgatctc tttaaagggt ggtcccctag cgatagagca ctcgatcttc
     9061 ccagaaaaag aggcagaagc agtagcagaa caggccacac aatcgcaagt gattaacgtc
     9121 cacacaggta tagggtttct ggaccatatg atacatgctc tggccaagca ttccggctgg
     9181 tcgctaatcg ttgagtgcat tggtgactta cacatagacg accatcacac cactgaagac
     9241 tgcgggattg ctctcggtca agcttttaaa gaggccctac tggcgcgtgg agtaaaaagg
     9301 tttggatcag gatttgcgcc tttggatgag gcactttcca gagcggtggt agatctttcg
     9361 aacaggccgt acgcagttgt cgaacttggt ttgcaaaggg agaaagtagg agatctctct
     9421 tgcgagatga tcccgcattt tcttgaaagc tttgcagagg ctagcagaat taccctccac
     9481 gttgattgtc tgcgaggcaa gaatgatcat caccgtagtg agagtgcgtt caaggctctt
     9541 gcggttgcca taagagaagc cacctcgccc aatggtacca acgatgttcc ctccaccaaa
     9601 ggtgttctta tgtagtgaca ccgattattt aaagctgcag catacgatat atatacatgt
     9661 gtatatatgt atacctatga atgtcagtaa gtatgtatac gaacagtatg atactgaaga
     9721 tgacaaggta atgcatcatt ctatacgtgt cattctgaac gaggcgcgct ttcctttttt
     9781 ctttttgctt tttctttttt tttctcttga actcgacgga tctatgcggt gtgaaatacc
     9841 gcacagatgc gtaaggagaa aataccgcat caggaaattg taaacgttaa tattttgtta
     9901 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     9961 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
    10021 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
    10081 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
    10141 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
    10201 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
    10261 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
    10321 cagggcgcgt cgcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
    10381 cgggcctctt cgctattacg ccag
//
