LOCUS       Exported                3865 bp DNA     linear   UNA 29-JAN-2019
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 12, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    34..433
                     /locus_tag="Native Promoter Region"
                     /label=Native Promoter Region
     misc_feature    34..433
                     /locus_tag="Native promoter"
                     /label=Native promoter
     misc_feature    434..519
                     /locus_tag="5UTR"
                     /label=5UTR
     misc_feature    434..519
                     /locus_tag="5UTR(1)"
                     /label=5UTR
                     /label=5UTR(1)
     misc_feature    479..480
                     /locus_tag="Loop 2 base deletion"
                     /label=Loop 2 base deletion
     misc_feature    500..501
                     /locus_tag="Loop 2 base deletion(1)"
                     /label=Loop 2 base deletion
                     /label=Loop 2 base deletion(1)
     misc_feature    520..621
                     /locus_tag="rpmE coding region"
                     /label=rpmE coding region
     misc_feature    610..621
                     /locus_tag="Linker"
                     /label=Linker
     misc_feature    622..1335
                     /locus_tag="SFGFP"
                     /label=SFGFP
     misc_feature    1248..1253
                     /locus_tag="BamHI-BglII Scar"
                     /label=BamHI-BglII Scar
     misc_feature    complement(1339..1344)
                     /locus_tag="BamHI-BglII Scar(3)"
                     /label=BamHI-BglII Scar
                     /label=BamHI-BglII Scar(3)
     misc_feature    1345..1712
                     /locus_tag="TrrnB"
                     /label=TrrnB
     misc_feature    complement(1381..1386)
                     /locus_tag="BamHI-BglII Scar(2)"
                     /label=BamHI-BglII Scar
                     /label=BamHI-BglII Scar(2)
     misc_feature    1747..2610
                     /locus_tag="CmR(EcoRI-KO) Cassette "
                     /label=CmR(EcoRI-KO) Cassette
                     
     misc_feature    2538..2543
                     /locus_tag="BamHI-BglII Scar(1)"
                     /label=BamHI-BglII Scar
                     /label=BamHI-BglII Scar(1)
     misc_feature    2893..3737
                     /locus_tag="p15A oripACYC"
                     /label=p15A oripACYC
ORIGIN
        1 cactttatgc ttccggctga attctaaaga tctcggcaag gcatgaaaca gcacatcgcc
       61 aatcggatga tgatagtaat ctgccgccca tagcagcaat cgccagacgg agtgagtaaa
      121 caccggctca ctatccagca cttcgactac cgcttttagc tcattgagcg gcagttcgct
      181 ggcatcgcta actgatacca caatcccgat gcgctcctgc tgtttgccaa acggcacgcg
      241 cacgcgacac ccagctttaa ccgtcatgcc ttctggcagc agatagtcaa aggtacgagg
      301 aagcggaacg ggcaaggcaa cgtgggcaac gggcatagca tcatcctgac ttgaaattcg
      361 gtgggttagt atacacattg ccgtagaaga gtgcggatca gtttgcatac gctggttaat
      421 ttctgtatga tttcgcgcct tcgtacgaaa tgatcgtatt gaagctatac ttttaacatg
      481 tctggcgtta gggctggaag cttaaaccga ggttttccca tgaaaaaaga tattcacccg
      541 aaatacgaag aaattactgc tagctgctct tgcggtaacg taatgaaaat ccgctccacc
      601 gttggtcatg gtggtggtgg tatgagcaaa ggagaagaac ttttcactgg agttgtccca
      661 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtccg tggagagggt
      721 gaaggtgatg ctacaaacgg aaaactcacc cttaaattta tttgcactac tggaaaacta
      781 cctgttccgt ggccaacact tgtcactact ctgacctatg gtgttcaatg cttttcccgt
      841 tatccggatc acatgaaacg gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      901 caggaacgca ctatatcttt caaagatgac gggacctaca agacgcgtgc tgaagtcaag
      961 tttgaaggtg atacccttgt taatcgtatc gagttaaagg gtattgattt taaagaagat
     1021 ggaaacattc ttggacacaa actcgagtac aactttaact cacacaatgt atacatcacg
     1081 gcagacaaac aaaagaatgg aatcaaagct aacttcaaaa ttcgccacaa cgttgaagat
     1141 ggttccgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
     1201 cttttaccag acaaccatta cctgtcgaca caatctgtcc tttcgaaaga tcccaacgaa
     1261 aagcgtgacc acatggtcct tcttgagttt gtaactgctg ctgggattac acatggcatg
     1321 gatgagctct acaaataagg atctgaagct tgggcccgaa caaaaactca tctcagaaga
     1381 ggatctgaat agcgccgtcg accatcatca tcatcatcat tgagtttaaa cggtctccag
     1441 cttggctgtt ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc
     1501 agaagcggtc tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac
     1561 cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat
     1621 gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1681 ctttcgtttt atctgttgtt tgtcggtgaa ctggatcctt actcgagtct agactgcagt
     1741 tgatcgggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
     1801 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     1861 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     1921 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     1981 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     2041 tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga cggtgagctg gtgatatggg
     2101 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     2161 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     2221 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     2281 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     2341 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     2401 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     2461 atgaattaca acagtactgc gatgagtggc agggcggggc gtaatttgat atcgagctcg
     2521 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga
     2581 acgctcggtt gccgccgggc gttttttatt ggtgagaatc caagcctccg atcaacgtct
     2641 cattttcgcc aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt
     2701 tattctgcga agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat
     2761 gctgccaact tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt
     2821 ttctatcagc tgtccctcct gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac
     2881 cgccggacat cagcgctagc ggagtgtata ctggcttact atgttggcac tgatgagggt
     2941 gtcagtgaag tgcttcatgt ggcaggagaa aaaaggctgc accggtgcgt cagcagaata
     3001 tgtgatacag gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac
     3061 tgcggcgagc ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga
     3121 tacttaacag ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc
     3181 tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata
     3241 aagataccag gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg
     3301 gtttaccggt gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca
     3361 gttccgggta ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg
     3421 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaaag
     3481 caccactggc agcagccact ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc
     3541 ggttaaggct aaactgaaag gacaagtttt ggtgactgcg ctcctccaag ccagttacct
     3601 cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt
     3661 tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt
     3721 aatcagataa aatatttcta gatttcagtg caatttatct cttcaaatgt agcacctgaa
     3781 gtcagcccca tacgatataa gttgtaattc tcatgtttga cagcttatca tcgataagct
     3841 tccgatggcg cgccgagagg cttta
//
