LOCUS       Exported                3519 bp DNA     linear   UNA 11-DEC-2021
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 12, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3519
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    34..68
                     /locus_tag="J23108"
                     /label=J23108
     misc_feature    69..159
                     /locus_tag="5'UTR Random control for L31"
                     /label=5'UTR Random control for L31
     misc_feature    145
                     /locus_tag="Mutated from pRAR058"
                     /label=Mutated from pRAR058
     misc_feature    146..159
                     /locus_tag="Mutated from pRAR058(1)"
                     /label=Mutated from pRAR058
                     /label=Mutated from pRAR058(1)
     misc_feature    160..173
                     /locus_tag="RBS-Strong"
                     /label=RBS-Strong
     misc_feature    complement(168..173)
                     /locus_tag="BamHI-BglII Scar(1)"
                     /label=BamHI-BglII Scar
                     /label=BamHI-BglII Scar(1)
     misc_feature    174..275
                     /locus_tag="rpmE coding region"
                     /label=rpmE coding region
     misc_feature    264..275
                     /locus_tag="Linker"
                     /label=Linker
     misc_feature    276..989
                     /locus_tag="SFGFP"
                     /label=SFGFP
     misc_feature    902..907
                     /locus_tag="BamHI-BglII Scar(4)"
                     /label=BamHI-BglII Scar
                     /label=BamHI-BglII Scar(4)
     misc_feature    complement(993..998)
                     /locus_tag="BamHI-BglII Scar(3)"
                     /label=BamHI-BglII Scar
                     /label=BamHI-BglII Scar(3)
     misc_feature    999..1366
                     /locus_tag="TrrnB"
                     /label=TrrnB
     misc_feature    complement(1035..1040)
                     /locus_tag="BamHI-BglII Scar(2)"
                     /label=BamHI-BglII Scar
                     /label=BamHI-BglII Scar(2)
     misc_feature    1401..2264
                     /locus_tag="CmR(EcoRI-KO) Cassette "
                     /label=CmR(EcoRI-KO) Cassette
                     
     misc_feature    2192..2197
                     /locus_tag="BamHI-BglII Scar"
                     /label=BamHI-BglII Scar
     misc_feature    2547..3391
                     /locus_tag="p15A oripACYC"
                     /label=p15A oripACYC
ORIGIN
        1 cactttatgc ttccggctga attctaaaga tctctgacag ctagctcagt cctaggtata
       61 atgctagcgg caggtttgac gggagatagg gcacgctagt cccggtgttg tctcatctgt
      121 ctcttacggt aactgtagat cgtcccatca tcaactccta ggaggaagga tctatgaaaa
      181 aagatattca cccgaaatac gaagaaatta ctgctagctg ctcttgcggt aacgtaatga
      241 aaatccgctc caccgttggt catggtggtg gtggtatgag caaaggagaa gaacttttca
      301 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
      361 tccgtggaga gggtgaaggt gatgctacaa acggaaaact cacccttaaa tttatttgca
      421 ctactggaaa actacctgtt ccgtggccaa cacttgtcac tactctgacc tatggtgttc
      481 aatgcttttc ccgttatccg gatcacatga aacggcatga ctttttcaag agtgccatgc
      541 ccgaaggtta tgtacaggaa cgcactatat ctttcaaaga tgacgggacc tacaagacgc
      601 gtgctgaagt caagtttgaa ggtgataccc ttgttaatcg tatcgagtta aagggtattg
      661 attttaaaga agatggaaac attcttggac acaaactcga gtacaacttt aactcacaca
      721 atgtatacat cacggcagac aaacaaaaga atggaatcaa agctaacttc aaaattcgcc
      781 acaacgttga agatggttcc gttcaactag cagaccatta tcaacaaaat actccaattg
      841 gcgatggccc tgtcctttta ccagacaacc attacctgtc gacacaatct gtcctttcga
      901 aagatcccaa cgaaaagcgt gaccacatgg tccttcttga gtttgtaact gctgctggga
      961 ttacacatgg catggatgag ctctacaaat aaggatctga agcttgggcc cgaacaaaaa
     1021 ctcatctcag aagaggatct gaatagcgcc gtcgaccatc atcatcatca tcattgagtt
     1081 taaacggtct ccagcttggc tgttttggcg gatgagagaa gattttcagc ctgatacaga
     1141 ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg
     1201 tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg
     1261 tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag
     1321 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaactggat ccttactcga
     1381 gtctagactg cagttgatcg ggcacgtaag aggttccaac tttcaccata atgaaataag
     1441 atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat
     1501 ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca
     1561 ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat
     1621 tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca
     1681 cattcttgcc cgcctgatga atgctcatcc ggaatttcgt atggcaatga aagacggtga
     1741 gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac
     1801 gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc
     1861 gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa
     1921 tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc
     1981 caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga
     2041 caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt
     2101 cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt
     2161 tgatatcgag ctcgcttgga ctcctgttga tagatccagt aatgacctca gaactccatc
     2221 tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccaagcc
     2281 tccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta tcaacaggga
     2341 caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat tcggcgcaaa
     2401 gtgcgtcggg tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct
     2461 ccagtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt
     2521 aacggcaaaa gcaccgccgg acatcagcgc tagcggagtg tatactggct tactatgttg
     2581 gcactgatga gggtgtcagt gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt
     2641 gcgtcagcag aatatgtgat acaggatata ttccgcttcc tcgctcactg actcgctacg
     2701 ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa cggggcggag atttcctgga
     2761 agatgccagg aagatactta acagggaagt gagagggccg cggcaaagcc gtttttccat
     2821 aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac
     2881 ccgacaggac tataaagata ccaggcgttt ccccctggcg gctccctcgt gcgctctcct
     2941 gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc
     3001 acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc
     3061 ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga
     3121 aagacatgca aaagcaccac tggcagcagc cactggtaat tgatttagag gagttagtct
     3181 tgaagtcatg cgccggttaa ggctaaactg aaaggacaag ttttggtgac tgcgctcctc
     3241 caagccagtt acctcggttc aaagagttgg tagctcagag aaccttcgaa aaaccgccct
     3301 gcaaggcggt tttttcgttt tcagagcaag agattacgcg cagaccaaaa cgatctcaag
     3361 aagatcatct tattaatcag ataaaatatt tctagatttc agtgcaattt atctcttcaa
     3421 atgtagcacc tgaagtcagc cccatacgat ataagttgta attctcatgt ttgacagctt
     3481 atcatcgata agcttccgat ggcgcgccga gaggcttta
//
