LOCUS       Exported                5687 bp DNA     circular SYN 30-OCT-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 12, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4588
                     /dnas_title="Exported"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /note="/dnas_title=."
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     enhancer        234..613
                     /label=CMV enhancer
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     promoter        614..817
                     /label=CMV promoter
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     Msc             862..879
                     /label=T7 promoter
                     /note="color: #FF0000"
                     /note="color: #ff0000"
     CDS             904..1677
                     /codon_start=1
                     /label=CAHS7_HYPDU_Boothby
                     /note="color: #FF0000"
                     /note="color: #ff0000"
                     /translation="MDYKDDDDKMMFLARNVSRVALRSASLSPAAIPQQQHAGVAAVY
                     AVRFASSSGSGRPADNWAESQKEKAKAGLKDAQAEVGKVAREVKDKAAGGIEQAKDAV
                     KQGANDLKRSGSRTFENAKDDIQAKAQHAKSDLKGAKHQAEGVVENVKEAAENAWEKT
                     KDVAENLKDKVQSPGGLADKAANAWETVKDRAQDAASEVKHKAGDLKDKAQQVIHDAT
                     TQSGDNRKQDQQQRRDSQGSQSGQNSRSRNGCLWFCCVVL"
     promoter        1690..1737
                     /label=EM7 promoter
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     CDS             1756..2130
                     /label=BleoR
                     /note="color: #b7e6d7; direction: RIGHT"
                     /note="color: #------; direction: RIGHT"
     polyA_signal    2260..2381
                     /label=SV40 poly(A) signal
                     /note="color: #b4abac"
                     /note="color: #b4abac"
     primer_bind     complement(2430..2446)
                     /label=M13 rev
                     /note="color: #a020f0; direction: LEFT"
                     /note="color: #------; direction: LEFT"
     protein_bind    2454..2470
                     /label=lac operator
                     /note="color: #31849b"
                     /note="color: #31849b"
     promoter        complement(2478..2508)
                     /label=lac promoter
                     /note="color: #c6c9d1; direction: LEFT"
                     /note="color: #------; direction: LEFT"
     protein_bind    2523..2544
                     /label=CAP binding site
                     /note="color: #31849b"
                     /note="color: #31849b"
     rep_origin      complement(2832..3420)
                     /direction=LEFT
                     /label=ori
                     /note="color: #ffef86"
                     /note="color: #ffef86"
     CDS             complement(3591..4451)
                     /label=AmpR
                     /note="color: #b7e6d7; direction: LEFT"
                     /note="color: #------; direction: LEFT"
     promoter        complement(4452..4556)
                     /label=AmpR promoter
                     /note="color: #c6c9d1; direction: LEFT"
                     /note="color: #------; direction: LEFT"
ORIGIN      
        1 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
      841 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcc
      901 accatggatt acaaggatga tgatgataag atgatgtttc tggcccgcaa tgtaagcaga
      961 gtggctcttc gatctgcgag cctctctcct gcagcgatcc cacagcagca gcatgcagga
     1021 gtggctgccg tctatgcggt caggttcgcc tcatcatccg ggtcagggcg gcccgctgac
     1081 aactgggccg agtctcaaaa ggagaaggcc aaagccggcc tcaaggacgc tcaggccgag
     1141 gtcgggaagg tggctagaga agttaaggac aaggccgctg gagggatcga gcaggccaag
     1201 gatgccgtga agcagggagc caatgacctg aaacggtcag ggagtaggac ctttgaaaat
     1261 gccaaggatg acattcaggc gaaagcccag cacgcgaaat ccgacctgaa gggggcgaag
     1321 caccaggccg aaggcgtcgt tgaaaatgtg aaagaagcag cagaaaatgc atgggagaag
     1381 acaaaggacg tcgctgagaa cctgaaagac aaggtgcaat ccccgggtgg gctcgcagac
     1441 aaagctgcga atgcctggga gacagtaaag gatagggctc aggacgctgc ctctgaagtt
     1501 aagcacaagg ctggcgacct gaaagataag gcccagcagg taattcacga tgcgaccaca
     1561 caaagcggag acaataggaa gcaggatcag cagcaacgaa gagacagcca gggttctcag
     1621 tcaggtcaga attctcggtc tcggaacggg tgtttgtggt tttgttgtgt ggtattgtga
     1681 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     1741 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
     1801 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     1861 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     1921 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     1981 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
     2041 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     2101 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
     2161 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     2221 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     2281 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     2341 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     2401 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     2461 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     2521 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     2581 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2641 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2701 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2761 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2821 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2881 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2941 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3001 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     3061 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3121 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3181 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3241 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     3301 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3361 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3421 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3481 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3541 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3601 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3661 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3721 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3781 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3841 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3901 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3961 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4021 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4081 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4141 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4201 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4261 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4321 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4381 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4441 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4501 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4561 tttccccgaa aagtgccacc tgacgtcg
//

