LOCUS       Exported                5681 bp DNA     circular SYN 30-OCT-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 12, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4585
                     /dnas_title="Exported"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /note="/dnas_title=."
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     enhancer        234..613
                     /label=CMV enhancer
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     promoter        614..817
                     /label=CMV promoter
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     Msc             862..879
                     /label=T7 promoter
                     /note="color: #FF0000"
                     /note="color: #ff0000"
     CDS             904..1674
                     /codon_start=1
                     /label=MAHS_RAMVA_Tanaka
                     /note="color: #FF0000"
                     /note="color: #ff0000"
                     /translation="MDYKDDDDKMMSRYLLRDVQAVLRGVRKVAESSLKLETEKVSLR
                     LGDFRSQPSLRSVPASLTSRSQAFSLQEIAARAGVVLRGVQQQFRNVTGVNAAPVVAF
                     DNGSVLYSERIHSQSSQKQAPTTVPTGSVSNSPQPEGKANEAAERAKQFMNPPVAPMD
                     PVDKNEFVAMPEMGRSNGNGENKQAADFMKNQGDTDMDSQYAPDSSKNTKSVPTKEIV
                     AEDGSMSIEDIKKATQVTPGVAVKNEGV*GCLWFCCVVL"
     promoter        1687..1734
                     /label=EM7 promoter
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     CDS             1753..2127
                     /label=BleoR
                     /note="color: #b7e6d7; direction: RIGHT"
                     /note="color: #------; direction: RIGHT"
     polyA_signal    2257..2378
                     /label=SV40 poly(A) signal
                     /note="color: #b4abac"
                     /note="color: #b4abac"
     primer_bind     complement(2427..2443)
                     /label=M13 rev
                     /note="color: #a020f0; direction: LEFT"
                     /note="color: #------; direction: LEFT"
     protein_bind    2451..2467
                     /label=lac operator
                     /note="color: #31849b"
                     /note="color: #31849b"
     promoter        complement(2475..2505)
                     /label=lac promoter
                     /note="color: #c6c9d1; direction: LEFT"
                     /note="color: #------; direction: LEFT"
     protein_bind    2520..2541
                     /label=CAP binding site
                     /note="color: #31849b"
                     /note="color: #31849b"
     rep_origin      complement(2829..3417)
                     /direction=LEFT
                     /label=ori
                     /note="color: #ffef86"
                     /note="color: #ffef86"
     CDS             complement(3588..4448)
                     /label=AmpR
                     /note="color: #b7e6d7; direction: LEFT"
                     /note="color: #------; direction: LEFT"
     promoter        complement(4449..4553)
                     /label=AmpR promoter
                     /note="color: #c6c9d1; direction: LEFT"
                     /note="color: #------; direction: LEFT"
ORIGIN      
        1 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
      841 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcc
      901 accatggatt acaaggatga tgatgataag atgatgtcca gatacctgct gcgcgatgtc
      961 caggctgtat tacgcggagt tcgcaaagtg gccgagagta gcttaaagct ggagacggag
     1021 aaagtcagtc tgcggcttgg tgactttcgg tcacagcctt cccttcgcag tgtgcctgct
     1081 tccctcacaa gtcgatcaca ggcatttagc ctacaggaga tagctgctcg tgccggagtt
     1141 gttctgcgag gagtgcaaca acagttccga aacgtcactg gagtgaatgc cgctcctgtt
     1201 gtagcctttg ataatggatc agttctatac agtgaaagaa tccactcgca gagttcgcag
     1261 aagcaggccc cgactacagt accaacagga tccgtcagca attcccctca accggaagga
     1321 aaggcaaacg aagctgctga acgcgcaaaa cagtttatga atcctccagt tgcgccaatg
     1381 gatcctgtcg acaagaatga atttgtcgcc atgccggaga tgggtcgtag taatggaaat
     1441 ggagaaaaca aacaagctgc tgatttcatg aaaaaccaag gtgacaccga tatggattcc
     1501 cagtacgcgc ctgattcatc gaagaacacg aaatcggttc ccacgaagga aatcgttgct
     1561 gaagatggtt cgatgagcat tgaggatatc aagaaagcta cgcaggttac tcctggagtt
     1621 gcagttaaaa acgagggtgt ttaggggtgt ttgtggtttt gttgtgtggt attgtgatca
     1681 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     1741 aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
     1801 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
     1861 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
     1921 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
     1981 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
     2041 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
     2101 cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc
     2161 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     2221 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     2281 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     2341 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     2401 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     2461 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     2521 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     2581 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     2641 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     2701 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     2761 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     2821 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2881 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2941 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3001 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
     3061 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3121 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3181 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3241 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     3301 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3361 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3421 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3481 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3541 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     3601 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     3661 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     3721 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     3781 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     3841 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     3901 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     3961 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4021 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4081 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4141 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4201 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4261 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4321 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4381 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4441 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4501 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     4561 ccccgaaaag tgccacctga cgtcg
//

