LOCUS       Exported                4303 bp DNA     circular SYN 10-MAY-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 10, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4303
                     /mol_type="other DNA"
                     /note="/dnas_title=."
                     /note="color: #ffffff"
                     /note="/dnas_title=Exported"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     enhancer        234..613
                     /label=CMV enhancer
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     promoter        614..817
                     /label=CMV promoter
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     Msc             862..879
                     /label=T7 promoter
                     /note="color: #ff0000"
                     /note="color: #ff0000"
     CDS             904..1266
                     /codon_start=1
                     /label=Hsa_A0A1D1UNQ6_RAMVA_1
                     /note="color: #ff0000"
                     /note="color: #ff0000"
                     /translation="MDYKDDDDKMMNHPNMKKDQPVTLQTFKKGDKYHHKIVVEEAGYI
                     NDVIFRLGRETPGSYNGQQITVNYEEQGGALVGTVKYPAHNKVIHNTYEMDGQNLAKTS
                     ECEGVVHKRWYNKQQN"
     promoter        1405..1452
                     /label=EM7 promoter
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     CDS             1471..1845
                     /label=BleoR
                     /note="color: #------; direction: RIGHT"
                     /note="color: #b7e6d7; direction: RIGHT"
     polyA_signal    1975..2096
                     /label=SV40 poly(A) signal
                     /note="color: #b4abac"
                     /note="color: #b4abac"
     primer_bind     complement(2145..2161)
                     /label=M13 rev
                     /note="color: #------; direction: LEFT"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    2169..2185
                     /label=lac operator
                     /note="color: #31849b"
                     /note="color: #31849b"
     promoter        complement(2193..2223)
                     /label=lac promoter
                     /note="color: #------; direction: LEFT"
                     /note="color: #c6c9d1; direction: LEFT"
     protein_bind    2238..2259
                     /label=CAP binding site
                     /note="color: #31849b"
                     /note="color: #31849b"
     rep_origin      complement(2547..3135)
                     /direction=LEFT
                     /label=ori
                     /note="color: #ffef86"
                     /note="color: #ffef86"
     CDS             complement(3306..4166)
                     /label=AmpR
                     /note="color: #------; direction: LEFT"
                     /note="color: #b7e6d7; direction: LEFT"
     promoter        complement(4167..4271)
                     /label=AmpR promoter
                     /note="color: #------; direction: LEFT"
                     /note="color: #c6c9d1; direction: LEFT"
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
      841 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcc
      901 accatggatt acaaggatga tgatgataag atgatgaatc atccaaatat gaaaaaagac
      961 cagccagtga ccctccagac cttcaagaag ggggacaagt atcatcataa gattgtggtg
     1021 gaggaggcag ggtatattaa tgacgtgatt ttcaggttgg ggagggagac cccagggtct
     1081 tataatgggc agcagattac tgtgaattat gaggagcagg gtggggcatt ggtggggact
     1141 gtcaagtatc cagcacataa caaggtgatt cataacacct atgagatgga tggacagaac
     1201 ttggcaaaga cctctgagtg tgagggagtg gtccataaga ggtggtataa caagcagcag
     1261 aactgagggt gtttgtggtt ttgttgacaa ggatgatgat gataagatgg gtgtgggtgt
     1321 ttgtggtttt gttgacaagg atgatgatga taagatgggt gtgggtgttt gtggttttgt
     1381 tgtgtggtat tgtgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     1441 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     1501 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     1561 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     1621 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     1681 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     1741 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     1801 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     1861 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     1921 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     1981 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     2041 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     2101 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     2161 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     2221 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     2281 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     2341 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     2401 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2461 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2521 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2581 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2641 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2701 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     2761 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2821 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2881 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2941 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     3001 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3061 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3121 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3181 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3241 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3301 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3361 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3421 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     3481 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3541 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3601 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3661 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3721 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     3781 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     3841 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     3901 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     3961 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4021 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4081 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4141 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4201 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4261 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcg
//
