LOCUS       Exported                5780 bp    ds-DNA  circular SYN 30-OCT-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 30, 2019 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     enhancer        234..613
                     /label="CMV enhancer"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     promoter        614..817
                     /label="CMV promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     polyA_signal    1793..2017
                     /label="bGH poly(A) signal"
                     /note="color: #b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
     rep_origin      2063..2491
                     /label="f1 ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     promoter        2505..2834
                     /label="SV40 promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     rep_origin      2685..2820
                     /label="SV40 ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     promoter        2882..2929
                     /label="EM7 promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     CDS             2948..3322
                     /label="BleoR"
                     /note="color: #b7e6d7; direction: RIGHT"
                     /ApEinfo_fwdcolor="#b7e6d7; direction: RIGHT"
                     /ApEinfo_revcolor="#b7e6d7; direction: RIGHT"
     polyA_signal    3452..3573
                     /label="SV40 poly(A) signal"
                     /note="color: #b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
     primer_bind     complement(3622..3638)
                     /label="M13 rev"
                     /note="color: #a020f0; direction: LEFT"
                     /ApEinfo_fwdcolor="#a020f0; direction: LEFT"
                     /ApEinfo_revcolor="#a020f0; direction: LEFT"
     protein_bind    3646..3662
                     /label="lac operator"
                     /note="color: #31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /ApEinfo_revcolor="#31849b"
     promoter        complement(3670..3700)
                     /label="lac promoter"
                     /note="color: #c6c9d1; direction: LEFT"
                     /ApEinfo_fwdcolor="#c6c9d1; direction: LEFT"
                     /ApEinfo_revcolor="#c6c9d1; direction: LEFT"
     protein_bind    3715..3736
                     /label="CAP binding site"
                     /note="color: #31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /ApEinfo_revcolor="#31849b"
     rep_origin      complement(4024..4612)
                     /direction=LEFT
                     /label="ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     CDS             complement(4783..5643)
                     /label="AmpR"
                     /note="color: #b7e6d7; direction: LEFT"
                     /ApEinfo_fwdcolor="#b7e6d7; direction: LEFT"
                     /ApEinfo_revcolor="#b7e6d7; direction: LEFT"
     promoter        complement(5644..5748)
                     /label="AmpR promoter"
                     /note="color: #c6c9d1; direction: LEFT"
                     /ApEinfo_fwdcolor="#c6c9d1; direction: LEFT"
                     /ApEinfo_revcolor="#c6c9d1; direction: LEFT"
     CDS             904..1773
                     /label="pST_Baker_TJ_190"
                     /note="color: #FF0000"
                     /translation="MGGSGWGPLREVLEEALRALEELARERGDKEKVEEVKKEKEEEEE
                     EKEKNKEEAKELAELIKENPLVRVLVEALRALVELAEERGDLEKVEEVAEEVKEEIEEL
                     RENKEEAKELAELIKENPLVRVLVEALRALVELAEERGDLEKVEEVAEEVKEEIEELRE
                     NKEEAKELAELIKENPLVRVLVEALRALVELAEERGDLEKVEEVAEEVKEEIEELRENK
                     EEAKELAELIKENPLVRVLVEALRALVELAEERGDLEKVEEVAEEVKEEIEELRE*GSG
                     DYKDDDDK*"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
     source          1..5780
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
     Msc             862..879
                     /label="T7 promoter"
                     /note="color: #FF0000"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
      841 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcc
      901 accatgggtg gcagcggatg gggacctctg agggaagtgt tggaagaggc ccttcgcgcg
      961 cttgaagaac ttgctcggga gcgaggtgac aaagagaaag ttgaagaggt caagaaagaa
     1021 aaggaagagg aagaggaaga aaaggaaaag aataaagaag aggcgaagga gttggcagaa
     1081 cttatcaagg aaaatcctct ggtacgcgta ttggttgagg ccctcagagc ccttgttgaa
     1141 cttgctgagg agcgtgggga tctcgagaaa gtggaagagg tggccgaaga agtgaaagaa
     1201 gagatagaag agcttaggga aaataaagaa gaagcgaaag aactcgctga acttatcaag
     1261 gagaaccccc tcgttcgggt cctcgttgag gcactgaggg cgctcgttga acttgccgaa
     1321 gaacgcggtg acttggaaaa ggtagaggaa gttgccgaag aagttaaaga agaaatcgaa
     1381 gaattgagag agaacaaaga agaagctaaa gagctcgccg agctcattaa ggaaaaccca
     1441 ctcgtcaggg ttcttgtaga ggcgctccgc gcacttgttg aattggctga agagcgcgga
     1501 gaccttgaaa aggtcgaaga agtcgcagaa gaagttaagg aagaaattga agagctcagg
     1561 gagaataagg aagaggccaa agaattggct gaattgatca aagaaaatcc gcttgtacgg
     1621 gttcttgtag aagccttgcg tgcacttgtc gaactcgccg aagaacgggg cgatctcgaa
     1681 aaggttgagg aagttgcaga agaagttaaa gaagagatag aggagcttcg ggaataagga
     1741 tctggggact acaaggacga cgacgacaag tgaacccgct gatcagcctc gactgtgcct
     1801 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     1861 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     1921 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     1981 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     2041 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     2101 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     2161 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc
     2221 atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     2281 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     2341 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     2401 tcggtctatt cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat
     2461 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     2521 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     2581 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     2641 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     2701 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     2761 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     2821 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     2881 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     2941 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     3001 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     3061 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     3121 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     3181 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     3241 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     3301 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     3361 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     3421 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     3481 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     3541 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     3601 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     3661 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     3721 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     3781 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3841 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3901 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3961 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4021 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4081 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4141 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4201 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4261 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4321 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4381 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4441 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     4501 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4561 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4621 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4681 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4741 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4801 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4861 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4921 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4981 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5041 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5101 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5161 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5221 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5281 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5341 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5401 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5461 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     5521 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     5581 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5641 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     5701 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     5761 aaaagtgcca cctgacgtcg
//
