LOCUS       Exported                7268 bp    ds-DNA  circular SYN 30-OCT-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 30, 2019 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     enhancer        234..613
                     /label="CMV enhancer"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     promoter        614..817
                     /label="CMV promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     polyA_signal    3281..3505
                     /label="bGH poly(A) signal"
                     /note="color: #b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
     rep_origin      3551..3979
                     /label="f1 ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     promoter        3993..4322
                     /label="SV40 promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     rep_origin      4173..4308
                     /label="SV40 ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     promoter        4370..4417
                     /label="EM7 promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     CDS             4436..4810
                     /label="BleoR"
                     /note="color: #b7e6d7; direction: RIGHT"
                     /ApEinfo_fwdcolor="#b7e6d7; direction: RIGHT"
                     /ApEinfo_revcolor="#b7e6d7; direction: RIGHT"
     polyA_signal    4940..5061
                     /label="SV40 poly(A) signal"
                     /note="color: #b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
     primer_bind     complement(5110..5126)
                     /label="M13 rev"
                     /note="color: #a020f0; direction: LEFT"
                     /ApEinfo_fwdcolor="#a020f0; direction: LEFT"
                     /ApEinfo_revcolor="#a020f0; direction: LEFT"
     protein_bind    5134..5150
                     /label="lac operator"
                     /note="color: #31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /ApEinfo_revcolor="#31849b"
     promoter        complement(5158..5188)
                     /label="lac promoter"
                     /note="color: #c6c9d1; direction: LEFT"
                     /ApEinfo_fwdcolor="#c6c9d1; direction: LEFT"
                     /ApEinfo_revcolor="#c6c9d1; direction: LEFT"
     protein_bind    5203..5224
                     /label="CAP binding site"
                     /note="color: #31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /ApEinfo_revcolor="#31849b"
     rep_origin      complement(5512..6100)
                     /direction=LEFT
                     /label="ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     CDS             complement(6271..7131)
                     /label="AmpR"
                     /note="color: #b7e6d7; direction: LEFT"
                     /ApEinfo_fwdcolor="#b7e6d7; direction: LEFT"
                     /ApEinfo_revcolor="#b7e6d7; direction: LEFT"
     promoter        complement(7132..7236)
                     /label="AmpR promoter"
                     /note="color: #c6c9d1; direction: LEFT"
                     /ApEinfo_fwdcolor="#c6c9d1; direction: LEFT"
                     /ApEinfo_revcolor="#c6c9d1; direction: LEFT"
     CDS             904..3261
                     /label="pST_H2FLK4_CAEEL"
                     /note="color: #FF0000"
                     /translation="MLSNPNLKRLVMNQWRPAQILLLEQPGPSRRFVENTTHNKMSFMD
                     KVKDTANSAYESVENTAVGAKDGVVVAFNTVGEKIGGAYDSTKQYASDKVSAAGDYASE
                     AGKKVGDAEDSMIQGAYDAKDSAKESASNAGNKISNAFTSLKDSITGDNAHKTGEALND
                     NYESVKESASEAADRLKEPAQNTADNAKDLANQTGEEMSDAFESVKQLTSDTAENAKEN
                     AHDAADTAKSYKDKAGEKASGILDSFKAHASDAKDSVENKASDAYNSAKDKAGDAWDST
                     KDAAGDAKDKTKSFTEKVGDKISGAYDSVKEKASDVADSFKAHSTDSKDNVENKAADAY
                     NTAKDKASDAWDKTKDKAGEAKDKMGDAWDTTKDKAGDAWDTTKDKAGDGKGKAGDAWD
                     TTKDKASDAWDTTKDKAGEAKDKMGEAWDHTKDKAGEAKDKASDAADDAQGKSKSMTEK
                     IGDSISGAWESTKDTAVSAKDKTAEAAGKVGDSISGAYDTVKEKASDIADSFKAHSTDS
                     KDNVENKAADAYNSAKDKASDAWDKTKDKAGDAKDKAADAWDTTKDKAGDAWDSTKDHA
                     ADAKDKASDAAGDAKDKSKSLTEKAGDAISGAYDSVKEKASDIADSFKAHSTNSKDNVE
                     NKASDAYNSAKDKASDAWDKTKDKAGEAKDKAGDAWDNTKDKAGNAWDSTKDKASDAWD
                     TTKDKASDAKESAGDAADSAKDKSKSITETIGDKISGAYDSVKEKASDVADSFKAHSSD
                     AQDTVEVSHHKYFNVFSSFE*GSGDYKDDDDK*"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
     source          1..7268
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
     Msc             862..879
                     /label="T7 promoter"
                     /note="color: #FF0000"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
      841 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcc
      901 accatgttga gtaatccgaa tttgaaacgg ctggtaatga accaatggcg gcccgcacaa
      961 atattgttgc tggaacaacc ggggccaagc agaagatttg ttgaaaacac tacgcacaac
     1021 aaaatgagct ttatggataa ggtcaaagac acggctaata gtgcatatga aagtgttgag
     1081 aacacagctg ttggcgccaa agatggggtc gttgtagctt tcaacactgt aggagagaaa
     1141 attggtgggg cgtacgactc cacgaaacaa tatgcgtctg ataaagtcag tgcagccggc
     1201 gattacgcaa gtgaagctgg gaagaaagtg ggcgatgcag aagactctat gatacaaggg
     1261 gcttatgatg ccaaggattc cgctaaagaa tctgccagta atgcaggcaa taagattagc
     1321 aatgccttta caagcctcaa agatagcatt accggcgata atgctcataa aaccggtgaa
     1381 gcgcttaatg ataactatga atcagtgaag gaaagcgcga gcgaagcagc cgatcgactc
     1441 aaggaaccgg cccaaaacac cgctgataat gcaaaggatc tggccaatca aaccggggaa
     1501 gaaatgtccg acgcctttga aagcgtcaag caacttacat ccgatacagc agaaaatgca
     1561 aaggagaatg cacatgatgc ggccgatact gccaagagtt ataaagataa ggcgggagaa
     1621 aaggcttcag gtattctcga ctcctttaaa gcgcacgctt cagatgccaa agacagcgtc
     1681 gaaaataagg ctagcgatgc ttataacagc gccaaagata aagcgggaga tgcatgggat
     1741 tctactaaag atgcagcggg tgatgccaaa gacaaaacca aatcctttac cgaaaaggtt
     1801 ggggacaaaa taagtggagc atatgattct gttaaagaga aagccagcga cgtcgcagat
     1861 tctttcaagg cccatagtac cgactcaaaa gataatgtcg aaaacaaagc agccgatgct
     1921 tacaacacgg ctaaagataa ggcttccgat gcttgggaca aaactaaaga taaggctggt
     1981 gaagcaaaag acaagatggg tgatgcgtgg gatactacga aagataaagc gggtgatgcc
     2041 tgggacacaa cgaaagataa agctggggat ggaaaaggaa aagccggaga tgcatgggat
     2101 actactaaag acaaggcaag cgatgcctgg gatacaacta aagataaagc cggcgaagca
     2161 aaggacaaaa tgggtgaagc atgggatcac actaaagaca aagccggtga agctaaagat
     2221 aaagcgagcg atgccgctga cgatgcccag ggcaaaagca aatcaatgac tgagaagatc
     2281 ggggacagca tctcaggcgc ttgggagtct accaaagaca cagctgtttc agccaaagat
     2341 aaaaccgcgg aagccgcagg taaggtggga gatagtatct ccggcgcgta tgatacggtt
     2401 aaggaaaagg ccagcgacat tgctgatagc ttcaaagctc atagcacaga ttctaaagat
     2461 aatgttgaaa ataaagccgc ggatgcctat aactccgcta aggataaagc ttccgatgct
     2521 tgggataaga ctaaagataa ggctggcgat gcaaaagata aagccgcgga tgcctgggat
     2581 accacaaaag acaaggcggg cgatgcgtgg gactccacta aggatcatgc agcagatgct
     2641 aaggataagg caagtgatgc cgcgggagat gccaaagata aaagcaagtc tctcacagag
     2701 aaagctgggg atgcgatctc tggagcatac gatagtgtca aagagaaagc ttctgacatt
     2761 gcagattcct ttaaagctca cagcaccaac agcaaagaca atgttgaaaa taaagcttca
     2821 gacgcttata actccgcgaa ggataaagca tcagacgcct gggataaaac taaagataaa
     2881 gcaggtgaag ctaaagataa agctggggat gcttgggaca atacaaaaga taaagccggg
     2941 aatgcctggg attctactaa agacaaagca tctgatgcgt gggatacaac aaaagacaaa
     3001 gcttctgatg ccaaggaaag cgcgggagat gcagcagatt cagccaagga taaatcaaag
     3061 tcaattacag aaaccattgg cgataaaatt agcggagcat atgatagcgt caaagaaaag
     3121 gcgtcagatg tggcagattc cttcaaagca cacagcagcg acgcgcaaga taccgtcgaa
     3181 gtgtcccatc ataaatattt taatgttttc tccagcttcg aataaggatc tggggactac
     3241 aaggacgacg acgacaagtg aacccgctga tcagcctcga ctgtgccttc tagttgccag
     3301 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     3361 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     3421 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     3481 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     3541 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     3601 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     3661 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcggggcat ccctttaggg
     3721 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     3781 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     3841 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     3901 tttgatttat aagggatttt ggggatttcg gcctattggt taaaaaatga gctgatttaa
     3961 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     4021 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     4081 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4141 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4201 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4261 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     4321 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
     4381 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
     4441 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
     4501 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
     4561 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     4621 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
     4681 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     4741 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
     4801 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     4861 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     4921 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     4981 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     5041 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     5101 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     5161 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     5221 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     5281 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     5341 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5401 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5461 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5521 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5581 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5641 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5701 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5761 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5821 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5881 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5941 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6001 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     6061 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6121 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6181 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6241 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6301 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6361 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6421 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6481 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6541 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6601 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6661 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6721 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6781 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6841 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     6901 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     6961 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     7021 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     7081 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7141 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     7201 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     7261 tgacgtcg
//
