LOCUS       Exported                5378 bp DNA     circular SYN 12-APR-2022
DEFINITION  .
ACCESSION   <unknown
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 12, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5378
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     enhancer        234..613
                     /label=CMV enhancer
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     promoter        614..817
                     /label=CMV promoter
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     Msc             862..879
                     /label=T7 promoter
                     /note="color: #FF0000"
                     /note="color: #ff0000"
     CDS             904..906
                     /codon_start=1
                     /label=Start
                     /note="color: #d7acf6"
                     /note="color: #d7acf6"
                     /translation="M"
     CDS             907..1173
                     /codon_start=1
                     /label=pST_CAHS1_PARRC_101-189
                     /label=CAHS1_PARRC_297-564
                     /note="color: #6f3471"
                     /note="color: #6f3471"
                     /translation="AKTREQEMLSANYQKEVERKTEAYRKQQEVEADKIRKELEKQHLR
                     DVEFRKDIVEMAIENQKKMIDVESRYAKKDMDRERVKVRMMLEQ"
     CDS             1174..1176
                     /codon_start=1
                     /label=stop opal TGA
                     /note="color: #5ad917"
                     /note="color: #5ad917"
                     /translation=""
     CDS             1177..1371
                     /codon_start=1
                     /label=DNA Spacer
                     /note="color: #c95d72"
                     /note="color: #c95d72"
                     /translation="*IAN*NLTAYVTETIQTPHVDTERIDAYLLP*KRTIIQLGT*CVR
                     PSSTPISVGPVQELEEHPPL"
     polyA_signal    2192..2416
                     /label=bGH poly(A) signal
                     /note="color: #b4abac"
                     /note="color: #b4abac"
     rep_origin      2462..2890
                     /label=f1 ori
                     /note="color: #ffef86"
                     /note="color: #ffef86"
     promoter        2904..3233
                     /label=SV40 promoter
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     rep_origin      3084..3219
                     /label=SV40 ori
                     /note="color: #ffef86"
                     /note="color: #ffef86"
     promoter        3281..3328
                     /label=EM7 promoter
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     CDS             3347..3721
                     /label=BleoR
                     /note="color: #b7e6d7; direction: RIGHT"
                     /note="color: #------; direction: RIGHT"
     polyA_signal    3851..3972
                     /label=SV40 poly(A) signal
                     /note="color: #b4abac"
                     /note="color: #b4abac"
     primer_bind     complement(4021..4037)
                     /label=M13 rev
                     /note="color: #a020f0; direction: LEFT"
                     /note="color: #------; direction: LEFT"
     protein_bind    4045..4061
                     /label=lac operator
                     /note="color: #31849b"
                     /note="color: #31849b"
     promoter        complement(4069..4099)
                     /label=lac promoter
                     /note="color: #c6c9d1; direction: LEFT"
                     /note="color: #------; direction: LEFT"
     protein_bind    4114..4135
                     /label=CAP binding site
                     /note="color: #31849b"
                     /note="color: #31849b"
     rep_origin      complement(4423..5011)
                     /direction=LEFT
                     /label=ori
                     /note="color: #ffef86"
                     /note="color: #ffef86"
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
      841 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcc
      901 accatggcca aaacgcgcga acaggagatg ctgtctgcta actatcaaaa agaagttgag
      961 cgcaaaactg aagcctatcg gaagcagcaa gaagtggagg cagacaaaat tcggaaagag
     1021 ctggaaaagc agcatctgcg tgacgtggag ttccgcaaag atattgtaga gatggcaatc
     1081 gaaaaccaga aaaaaatgat tgatgtcgaa agccgttatg caaaaaaaga tatggaccgc
     1141 gaacgtgtga aagtccgtat gatgctcgag cagtgatgaa ttgccaattg aaatttgacg
     1201 gcctatgtca ctgaaactat ccaaacaccc catgtcgata ctgaacgtat cgacgcatac
     1261 ctccttcctt gaaaacgcac aatcatacaa ctgggcacat aatgcgtacg cccatctagt
     1321 acacccatct ctgtaggtcc agttcaagag ctggaagagc accctccact tacccgctga
     1381 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     1441 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     1501 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     1561 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     1621 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     1681 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     1741 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     1801 caagctctaa atcggggcat ccctttaggg ttccgattta gtgctttacg gcacctcgac
     1861 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     1921 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     1981 acaacactca accctatctc ggtctattct tttgatttat aagggatttt ggggatttcg
     2041 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     2101 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     2161 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     2221 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     2281 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     2341 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     2401 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     2461 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
     2521 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
     2581 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
     2641 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
     2701 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
     2761 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
     2821 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
     2881 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
     2941 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     3001 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     3061 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     3121 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     3181 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     3241 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     3301 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     3361 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     3421 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     3481 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3541 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     3601 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     3661 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3721 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3781 cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag
     3841 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     3901 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3961 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4021 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     4081 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     4141 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     4201 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     4261 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     4321 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     4381 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     4441 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     4501 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     4561 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     4621 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     4681 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     4741 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     4801 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     4861 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     4921 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     4981 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     5041 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     5101 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     5161 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     5221 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     5281 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     5341 tccgcgcaca tttccccgaa aagtgccacc tgacgtcg
//
