LOCUS       .                       5282 bp    ds-DNA  circular SYN 30-OCT-2019
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 30, 2019 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     enhancer        234..613
                     /label="CMV enhancer"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     promoter        614..817
                     /label="CMV promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     CDS             904..906
                     /label="Start"
                     /note="color: #d7acf6"
                     /ApEinfo_fwdcolor="#d7acf6"
                     /ApEinfo_revcolor="#d7acf6"
                     /translation="M"
     CDS             907..972
                     /label="pvLEA22mer_shuffle_5"
                     /note="color: #e64b08"
                     /ApEinfo_fwdcolor="#e64b08"
                     /ApEinfo_revcolor="#e64b08"
                     /translation="TKAEKAGEKGDKAEDKGETKAG"
     CDS             973..975
                     /label="stop opal TGA"
                     /note="color: #5ad917"
                     /ApEinfo_fwdcolor="#5ad917"
                     /ApEinfo_revcolor="#5ad917"
                     /translation="*"
     CDS             976..1275
                     /label="DNA Spacer_Longer"
                     /note="color: #1d13cc"
                     /ApEinfo_fwdcolor="#1d13cc"
                     /ApEinfo_revcolor="#1d13cc"
                     /translation="*IAN*NLTAYVTETIQTPHVDTERIDAYLLP*KRTIIQLGT*CVR
                     PSSTPISVGPVQELEEHPPLRRAVR*RLVRIAIQYER**LDEDKAVIEGAPSFIR"
     polyA_signal    2096..2320
                     /label="bGH poly(A) signal"
                     /note="color: #b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
     rep_origin      2366..2794
                     /label="f1 ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     promoter        2808..3137
                     /label="SV40 promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     rep_origin      2988..3123
                     /label="SV40 ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     promoter        3185..3232
                     /label="EM7 promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     CDS             3251..3625
                     /label="BleoR"
                     /note="color: #b7e6d7; direction: RIGHT"
                     /ApEinfo_fwdcolor="#b7e6d7; direction: RIGHT"
                     /ApEinfo_revcolor="#b7e6d7; direction: RIGHT"
     polyA_signal    3755..3876
                     /label="SV40 poly(A) signal"
                     /note="color: #b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
     primer_bind     complement(3925..3941)
                     /label="M13 rev"
                     /note="color: #a020f0; direction: LEFT"
                     /ApEinfo_fwdcolor="#a020f0; direction: LEFT"
                     /ApEinfo_revcolor="#a020f0; direction: LEFT"
     protein_bind    3949..3965
                     /label="lac operator"
                     /note="color: #31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /ApEinfo_revcolor="#31849b"
     promoter        complement(3973..4003)
                     /label="lac promoter"
                     /note="color: #c6c9d1; direction: LEFT"
                     /ApEinfo_fwdcolor="#c6c9d1; direction: LEFT"
                     /ApEinfo_revcolor="#c6c9d1; direction: LEFT"
     protein_bind    4018..4039
                     /label="CAP binding site"
                     /note="color: #31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /ApEinfo_revcolor="#31849b"
     rep_origin      complement(4327..4915)
                     /direction=LEFT
                     /label="ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     source          1..5282
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
     Msc             862..879
                     /label="T7 promoter"
                     /note="color: #FF0000"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
      841 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcc
      901 accatgacaa aagctgagaa ggccggcgag aagggagaca aggccgagga caagggcgag
      961 accaaggccg gatgatgaat tgccaattga aatttgacgg cctatgtcac tgaaactatc
     1021 caaacacccc atgtcgatac tgaacgtatc gacgcatacc tccttccttg aaaacgcaca
     1081 atcatacaac tgggcacata atgcgtacgc ccatctagta cacccatctc tgtaggtcca
     1141 gttcaagagc tggaagagca ccctccactt agacgtgccg taagatagag gttagtgaga
     1201 atagccatcc aatatgagcg ttgatagcta gacgaggata aggcagtgat agaaggggca
     1261 ccaagcttca ttcgcacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     1321 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     1381 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     1441 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     1501 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     1561 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     1621 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     1681 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga
     1741 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     1801 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     1861 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     1921 ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     1981 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     2041 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     2101 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     2161 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     2221 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     2281 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     2341 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
     2401 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt
     2461 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
     2521 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
     2581 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
     2641 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
     2701 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
     2761 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
     2821 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     2881 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     2941 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3001 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3061 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     3121 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     3181 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     3241 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     3301 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     3361 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3421 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3481 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3541 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3601 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3661 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3721 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3781 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3841 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3901 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3961 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4021 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     4081 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     4141 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4201 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4261 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     4321 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4381 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     4441 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4501 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4561 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     4621 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4681 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4741 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4801 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4861 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     4921 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     4981 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     5041 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     5101 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     5161 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     5221 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     5281 cg
//
