LOCUS       .                       5453 bp    ds-DNA  circular SYN 30-OCT-2019
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 30, 2019 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     enhancer        234..613
                     /label="CMV enhancer"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     promoter        614..817
                     /label="CMV promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     CDS             904..906
                     /label="Start"
                     /note="color: #d7acf6"
                     /ApEinfo_fwdcolor="#d7acf6"
                     /ApEinfo_revcolor="#d7acf6"
                     /translation="M"
     CDS             907..1443
                     /label="AfLEA1.1"
                     /note="color: #2cd9b8"
                     /ApEinfo_fwdcolor="#2cd9b8"
                     /ApEinfo_revcolor="#2cd9b8"
                     /translation="ELSSSKLNRSIFKRRSKMSEQGKLSRQEAGQRGGQARAEQLGHEG
                     YVEMGRKGGQARAEQLGHEGYQEMGQKGGQARAEQLGTEGYQEMGQKGGQKRAEQLGHE
                     GYQEIGQKGGQTRAEQLGTEGYQEMGQKGGQTRAEQLGHEGYVQMGKMGGEARKQQMSP
                     EDYAAMGQKGGLARQK"
     CDS             1444..1446
                     /label="stop opal TGA"
                     /note="color: #5ad917"
                     /ApEinfo_fwdcolor="#5ad917"
                     /ApEinfo_revcolor="#5ad917"
                     /translation="*"
     polyA_signal    2267..2491
                     /label="bGH poly(A) signal"
                     /note="color: #b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
     rep_origin      2537..2965
                     /label="f1 ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     promoter        2979..3308
                     /label="SV40 promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     rep_origin      3159..3294
                     /label="SV40 ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     promoter        3356..3403
                     /label="EM7 promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     CDS             3422..3796
                     /label="BleoR"
                     /note="color: #b7e6d7; direction: RIGHT"
                     /ApEinfo_fwdcolor="#b7e6d7; direction: RIGHT"
                     /ApEinfo_revcolor="#b7e6d7; direction: RIGHT"
     polyA_signal    3926..4047
                     /label="SV40 poly(A) signal"
                     /note="color: #b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
     primer_bind     complement(4096..4112)
                     /label="M13 rev"
                     /note="color: #a020f0; direction: LEFT"
                     /ApEinfo_fwdcolor="#a020f0; direction: LEFT"
                     /ApEinfo_revcolor="#a020f0; direction: LEFT"
     protein_bind    4120..4136
                     /label="lac operator"
                     /note="color: #31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /ApEinfo_revcolor="#31849b"
     promoter        complement(4144..4174)
                     /label="lac promoter"
                     /note="color: #c6c9d1; direction: LEFT"
                     /ApEinfo_fwdcolor="#c6c9d1; direction: LEFT"
                     /ApEinfo_revcolor="#c6c9d1; direction: LEFT"
     protein_bind    4189..4210
                     /label="CAP binding site"
                     /note="color: #31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /ApEinfo_revcolor="#31849b"
     rep_origin      complement(4498..5086)
                     /direction=LEFT
                     /label="ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     source          1..5453
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
     Msc             862..879
                     /label="T7 promoter"
                     /note="color: #FF0000"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
      841 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcc
      901 accatggagc tgtccagctc taagctgaac aggtccatct ttaagcgcag aagtaagatg
      961 tctgagcagg ggaagctgag tagacaggag gccggccaga gggggggaca ggcacgcgct
     1021 gagcagttgg gtcacgaagg ctacgttgag atgggccgca aaggcggcca ggctagggcc
     1081 gaacagctgg ggcacgaggg gtaccaggag atggggcaga agggcggcca ggcccgggcc
     1141 gagcagctgg gaacagaggg ctatcaggag atgggacaga aaggggggca gaagagagca
     1201 gagcagctgg gccacgaggg ctaccaggag atcggacaga agggcggcca gacccgggct
     1261 gagcagctgg gcaccgaggg gtatcaggag atgggccaga aaggagggca gaccagagca
     1321 gaacaattag gtcacgaagg ttatgttcaa atgggtaaaa tgggtggtga agctagaaaa
     1381 caacaaatgt cacctgaaga ttatgctgct atgggacaaa aaggaggttt agcaagacaa
     1441 aaatgaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     1501 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     1561 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     1621 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     1681 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     1741 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     1801 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     1861 gccggctttc cccgtcaagc tctaaatcgg ggcatccctt tagggttccg atttagtgct
     1921 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     1981 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     2041 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     2101 attttgggga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     2161 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     2221 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     2281 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     2341 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     2401 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     2461 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     2521 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     2581 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     2641 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     2701 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     2761 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     2821 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     2881 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     2941 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     3001 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     3061 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     3121 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     3181 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     3241 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     3301 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     3361 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     3421 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     3481 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     3541 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3601 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3661 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3721 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3781 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3841 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
     3901 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3961 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4021 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4081 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     4141 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4201 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4261 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4321 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4381 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4441 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4501 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4561 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4621 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4681 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4741 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     4801 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4861 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     4921 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     4981 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     5041 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     5101 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     5161 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     5221 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5281 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5341 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     5401 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcg
//
