LOCUS       .                       5303 bp    ds-DNA  circular SYN 30-OCT-2019
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 30, 2019 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     enhancer        234..613
                     /label="CMV enhancer"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     promoter        614..817
                     /label="CMV promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     CDS             904..906
                     /label="Start"
                     /note="color: #d7acf6"
                     /ApEinfo_fwdcolor="#d7acf6"
                     /ApEinfo_revcolor="#d7acf6"
                     /translation="M"
     CDS             907..993
                     /label="DrHD_V112A_R121D_Q125D_306-393"
                     /note="color: #13acb5"
                     /ApEinfo_fwdcolor="#13acb5"
                     /ApEinfo_revcolor="#13acb5"
                     /translation="AASDAKDKAQDVKADASDAADDAKDKAQD"
     CDS             994..996
                     /label="stop opal TGA"
                     /note="color: #5ad917"
                     /ApEinfo_fwdcolor="#5ad917"
                     /ApEinfo_revcolor="#5ad917"
                     /translation="*"
     CDS             997..1296
                     /label="DNA Spacer_Longer"
                     /note="color: #1d13cc"
                     /ApEinfo_fwdcolor="#1d13cc"
                     /ApEinfo_revcolor="#1d13cc"
                     /translation="*IAN*NLTAYVTETIQTPHVDTERIDAYLLP*KRTIIQLGT*CVR
                     PSSTPISVGPVQELEEHPPLRRAVR*RLVRIAIQYER**LDEDKAVIEGAPSFIR"
     polyA_signal    2117..2341
                     /label="bGH poly(A) signal"
                     /note="color: #b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
     rep_origin      2387..2815
                     /label="f1 ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     promoter        2829..3158
                     /label="SV40 promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     rep_origin      3009..3144
                     /label="SV40 ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     promoter        3206..3253
                     /label="EM7 promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     CDS             3272..3646
                     /label="BleoR"
                     /note="color: #b7e6d7; direction: RIGHT"
                     /ApEinfo_fwdcolor="#b7e6d7; direction: RIGHT"
                     /ApEinfo_revcolor="#b7e6d7; direction: RIGHT"
     polyA_signal    3776..3897
                     /label="SV40 poly(A) signal"
                     /note="color: #b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
     primer_bind     complement(3946..3962)
                     /label="M13 rev"
                     /note="color: #a020f0; direction: LEFT"
                     /ApEinfo_fwdcolor="#a020f0; direction: LEFT"
                     /ApEinfo_revcolor="#a020f0; direction: LEFT"
     protein_bind    3970..3986
                     /label="lac operator"
                     /note="color: #31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /ApEinfo_revcolor="#31849b"
     promoter        complement(3994..4024)
                     /label="lac promoter"
                     /note="color: #c6c9d1; direction: LEFT"
                     /ApEinfo_fwdcolor="#c6c9d1; direction: LEFT"
                     /ApEinfo_revcolor="#c6c9d1; direction: LEFT"
     protein_bind    4039..4060
                     /label="CAP binding site"
                     /note="color: #31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /ApEinfo_revcolor="#31849b"
     rep_origin      complement(4348..4936)
                     /direction=LEFT
                     /label="ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     source          1..5303
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
     Msc             862..879
                     /label="T7 promoter"
                     /note="color: #FF0000"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
      841 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcc
      901 accatggcgg ctagcgatgc taaagataaa gctcaagatg ttaaggcaga cgcgagcgac
      961 gcagccgacg acgcgaaaga caaagcccaa gattgatgaa ttgccaattg aaatttgacg
     1021 gcctatgtca ctgaaactat ccaaacaccc catgtcgata ctgaacgtat cgacgcatac
     1081 ctccttcctt gaaaacgcac aatcatacaa ctgggcacat aatgcgtacg cccatctagt
     1141 acacccatct ctgtaggtcc agttcaagag ctggaagagc accctccact tagacgtgcc
     1201 gtaagataga ggttagtgag aatagccatc caatatgagc gttgatagct agacgaggat
     1261 aaggcagtga tagaaggggc accaagcttc attcgcaccc gctgatcagc ctcgactgtg
     1321 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     1381 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     1441 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     1501 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     1561 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     1621 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     1681 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     1741 ggcatccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     1801 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     1861 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     1921 atctcggtct attcttttga tttataaggg attttgggga tttcggccta ttggttaaaa
     1981 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     2041 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     2101 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     2161 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     2221 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     2281 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     2341 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     2401 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     2461 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     2521 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     2581 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     2641 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
     2701 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     2761 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     2821 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
     2881 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     2941 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     3001 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3061 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     3121 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     3181 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     3241 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     3301 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     3361 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3421 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3481 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3541 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3601 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3661 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3721 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3781 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3841 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3901 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     3961 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     4021 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4081 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4141 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4201 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4261 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     4321 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     4381 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     4441 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     4501 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     4561 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     4621 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     4681 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     4741 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     4801 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     4861 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     4921 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     4981 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     5041 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     5101 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     5161 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     5221 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     5281 ccgaaaagtg ccacctgacg tcg
//
