LOCUS       .                       5600 bp    ds-DNA  circular SYN 30-OCT-2019
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 30, 2019 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     enhancer        234..613
                     /label="CMV enhancer"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     promoter        614..817
                     /label="CMV promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     CDS             904..906
                     /label="Start"
                     /note="color: #d7acf6"
                     /ApEinfo_fwdcolor="#d7acf6"
                     /ApEinfo_revcolor="#d7acf6"
                     /translation="M"
     CDS             907..1590
                     /label="DHR93_NCcapped"
                     /note="color: #49618d"
                     /ApEinfo_fwdcolor="#49618d"
                     /ApEinfo_revcolor="#49618d"
                     /translation="SEELERESEEAERRLQEARKRSEEARERGDLKELAEALIEEARAV
                     QELARVACERGNSEEAERASEKAQRVLEEARKVSEEAREQGDDEVLALALIAIALAVLA
                     LAEVACCRGNSEEAERASEKAQRVLEEARKVSEEAREQGDDEVLALALIAIALAVLALA
                     EVACCRGNKEEAERAYEDARRVEEEARKVKESAEEQGDSEVKRLAEEAEQLAREARRHV
                     QECRGN"
     CDS             1591..1593
                     /label="stop opal TGA"
                     /note="color: #5ad917"
                     /ApEinfo_fwdcolor="#5ad917"
                     /ApEinfo_revcolor="#5ad917"
                     /translation="*"
     polyA_signal    2414..2638
                     /label="bGH poly(A) signal"
                     /note="color: #b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
     rep_origin      2684..3112
                     /label="f1 ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     promoter        3126..3455
                     /label="SV40 promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     rep_origin      3306..3441
                     /label="SV40 ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     promoter        3503..3550
                     /label="EM7 promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     CDS             3569..3943
                     /label="BleoR"
                     /note="color: #b7e6d7; direction: RIGHT"
                     /ApEinfo_fwdcolor="#b7e6d7; direction: RIGHT"
                     /ApEinfo_revcolor="#b7e6d7; direction: RIGHT"
     polyA_signal    4073..4194
                     /label="SV40 poly(A) signal"
                     /note="color: #b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
     primer_bind     complement(4243..4259)
                     /label="M13 rev"
                     /note="color: #a020f0; direction: LEFT"
                     /ApEinfo_fwdcolor="#a020f0; direction: LEFT"
                     /ApEinfo_revcolor="#a020f0; direction: LEFT"
     protein_bind    4267..4283
                     /label="lac operator"
                     /note="color: #31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /ApEinfo_revcolor="#31849b"
     promoter        complement(4291..4321)
                     /label="lac promoter"
                     /note="color: #c6c9d1; direction: LEFT"
                     /ApEinfo_fwdcolor="#c6c9d1; direction: LEFT"
                     /ApEinfo_revcolor="#c6c9d1; direction: LEFT"
     protein_bind    4336..4357
                     /label="CAP binding site"
                     /note="color: #31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /ApEinfo_revcolor="#31849b"
     rep_origin      complement(4645..5233)
                     /direction=LEFT
                     /label="ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     source          1..5600
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
     Msc             862..879
                     /label="T7 promoter"
                     /note="color: #FF0000"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
      841 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcc
      901 accatgtccg aagaacttga acgcgaatca gaagaagctg aacgtcgact ccaagaagcg
      961 cgaaagagat ccgaagaagc acgtgaaaga ggagatctta aagaattggc cgaagccctt
     1021 attgaagaag ctcgggcggt ccaagaactc gcacgagtcg cctgcgaacg gggtaattcc
     1081 gaagaagcgg aacgagctag tgagaaagct cagcgcgttt tggaagaagc ccgcaaagtt
     1141 tctgaagaag cacgggaaca aggagatgat gaagtcttgg ccctcgcact gattgctata
     1201 gctcttgctg tcctcgcatt ggcggaagtc gcctgctgcc ggggtaattc tgaagaagca
     1261 gaacgcgctt ctgaaaaggc acaacgggtg ttggaagaag cccgaaaagt atcagaagaa
     1321 gctcgcgaac aaggggatga tgaagtcctc gcactggcac tgatagcaat tgcactcgcg
     1381 gtcctcgcgc tcgccgaagt tgcgtgctgc agaggtaata aagaagaagc ggaacgcgca
     1441 tatgaagatg cacggcgcgt cgaagaagaa gcccgtaaag taaaagaatc tgctgaagaa
     1501 caaggagatt cagaagtcaa aagattggcc gaagaagccg aacaattggc tcgggaagcg
     1561 cgccgccacg tacaagaatg tcgcgggaat tgaacccgct gatcagcctc gactgtgcct
     1621 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     1681 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     1741 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     1801 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     1861 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     1921 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     1981 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc
     2041 atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     2101 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     2161 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     2221 tcggtctatt cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat
     2281 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     2341 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     2401 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     2461 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     2521 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     2581 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     2641 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     2701 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     2761 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     2821 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     2881 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     2941 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     3001 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     3061 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     3121 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     3181 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     3241 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     3301 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     3361 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     3421 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     3481 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     3541 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     3601 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3661 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3721 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3781 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3841 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3901 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3961 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4021 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4081 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4141 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4201 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4261 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     4321 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4381 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4441 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4501 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4561 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4621 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4681 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4741 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4801 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4861 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     4921 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4981 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5041 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5101 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5161 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5221 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5281 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     5341 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     5401 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5461 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     5521 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     5581 aaaagtgcca cctgacgtcg
//
