LOCUS       .                       5543 bp    ds-DNA  circular SYN 30-OCT-2019
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 30, 2019 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     enhancer        234..613
                     /label="CMV enhancer"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     promoter        614..817
                     /label="CMV promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     CDS             904..906
                     /label="Start"
                     /note="color: #d7acf6"
                     /ApEinfo_fwdcolor="#d7acf6"
                     /ApEinfo_revcolor="#d7acf6"
                     /translation="M"
     CDS             907..1533
                     /label="HbDHN2"
                     /note="color: #ec6aa6"
                     /ApEinfo_fwdcolor="#ec6aa6"
                     /ApEinfo_revcolor="#ec6aa6"
                     /translation="AEQDHHKSHESETPVVGGGAAVETKDRGLFDFMGKKEEEKPQEEV
                     IVTEFEEKVQVSDPEPKEEENKHGLLEKLHRSDSSTSSSSDEEEGEGVEKNKKKKKGLK
                     EKIKDKITGDKEEKKEEDTTIPIEKVEKDEEAEDKKGFLEKIKEKLPGQQKKAEEETLP
                     APPPAENAHAEAASHEGDAKEKKGILEKIKEKIPGYHPKTEEEKKE"
     CDS             1534..1536
                     /label="stop opal TGA"
                     /note="color: #5ad917"
                     /ApEinfo_fwdcolor="#5ad917"
                     /ApEinfo_revcolor="#5ad917"
                     /translation="*"
     polyA_signal    2357..2581
                     /label="bGH poly(A) signal"
                     /note="color: #b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
     rep_origin      2627..3055
                     /label="f1 ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     promoter        3069..3398
                     /label="SV40 promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     rep_origin      3249..3384
                     /label="SV40 ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     promoter        3446..3493
                     /label="EM7 promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     CDS             3512..3886
                     /label="BleoR"
                     /note="color: #b7e6d7; direction: RIGHT"
                     /ApEinfo_fwdcolor="#b7e6d7; direction: RIGHT"
                     /ApEinfo_revcolor="#b7e6d7; direction: RIGHT"
     polyA_signal    4016..4137
                     /label="SV40 poly(A) signal"
                     /note="color: #b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
     primer_bind     complement(4186..4202)
                     /label="M13 rev"
                     /note="color: #a020f0; direction: LEFT"
                     /ApEinfo_fwdcolor="#a020f0; direction: LEFT"
                     /ApEinfo_revcolor="#a020f0; direction: LEFT"
     protein_bind    4210..4226
                     /label="lac operator"
                     /note="color: #31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /ApEinfo_revcolor="#31849b"
     promoter        complement(4234..4264)
                     /label="lac promoter"
                     /note="color: #c6c9d1; direction: LEFT"
                     /ApEinfo_fwdcolor="#c6c9d1; direction: LEFT"
                     /ApEinfo_revcolor="#c6c9d1; direction: LEFT"
     protein_bind    4279..4300
                     /label="CAP binding site"
                     /note="color: #31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /ApEinfo_revcolor="#31849b"
     rep_origin      complement(4588..5176)
                     /direction=LEFT
                     /label="ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     source          1..5543
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
     Msc             862..879
                     /label="T7 promoter"
                     /note="color: #FF0000"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
      841 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcc
      901 accatggccg agcaggacca ccacaagagc cacgagagcg agacacccgt ggtgggcggc
      961 ggagctgctg tggagacaaa ggacaggggc ctgttcgact tcatgggcaa gaaggaggag
     1021 gagaagcccc aggaggaggt gatcgtgacc gagttcgagg agaaggtgca ggtgagcgac
     1081 cccgagccca aggaggagga gaacaagcac ggcctgctgg agaagctgca caggagcgac
     1141 agcagcacca gcagcagcag cgacgaggag gagggcgagg gagtggagaa gaacaagaag
     1201 aagaagaagg gcctgaagga gaagatcaag gacaagatca ccggcgacaa ggaggagaag
     1261 aaggaggagg acaccaccat ccccatcgag aaggtggaga aggacgagga ggccgaggac
     1321 aagaagggct tcctcgagaa aataaaagag aaactgcccg gccagcagaa gaaggccgag
     1381 gaggagacac tgcccgctcc tcctcccgcc gagaacgctc atgccgaggc tgcctctcat
     1441 gagggcgacg ccaaggagaa gaagggcatc ttggagaaaa ttaaagaaaa gatccccggc
     1501 taccacccca agaccgagga ggagaagaag gagtgaaccc gctgatcagc ctcgactgtg
     1561 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     1621 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     1681 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     1741 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     1801 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     1861 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     1921 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     1981 ggcatccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     2041 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     2101 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     2161 atctcggtct attcttttga tttataaggg attttgggga tttcggccta ttggttaaaa
     2221 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     2281 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     2341 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     2401 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     2461 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     2521 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     2581 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     2641 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     2701 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     2761 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     2821 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     2881 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
     2941 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     3001 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     3061 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
     3121 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     3181 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     3241 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3301 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     3361 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     3421 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     3481 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     3541 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     3601 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3661 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3721 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3781 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3841 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3901 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3961 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4021 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4081 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4141 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4201 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     4261 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4321 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4381 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4441 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4501 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     4561 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     4621 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     4681 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     4741 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     4801 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     4861 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     4921 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     4981 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     5041 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     5101 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     5161 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     5221 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     5281 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     5341 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     5401 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     5461 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     5521 ccgaaaagtg ccacctgacg tcg
//
