LOCUS       Exported                5300 bp DNA     circular SYN 12-APR-2022
DEFINITION  .
ACCESSION   <unknown
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 12, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5300
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     enhancer        234..613
                     /label=CMV enhancer
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     promoter        614..817
                     /label=CMV promoter
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     Msc             862..879
                     /label=T7 promoter
                     /note="color: #FF0000"
                     /note="color: #ff0000"
     CDS             904..906
                     /codon_start=1
                     /label=Start
                     /note="color: #d7acf6"
                     /note="color: #d7acf6"
                     /translation="M"
     CDS             907..1095
                     /codon_start=1
                     /label=pST_APOE_HUMAN_E2_255-317
                     /label=APOE_HUMAN_E2_759-948
                     /note="color: #c465b7"
                     /note="color: #c465b7"
                     /translation="AEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVE
                     KVQAAVGTSAAPVPSDNH"
     CDS             1096..1098
                     /codon_start=1
                     /label=stop opal TGA
                     /note="color: #5ad917"
                     /note="color: #5ad917"
                     /translation=""
     CDS             1099..1293
                     /codon_start=1
                     /label=DNA Spacer
                     /note="color: #c95d72"
                     /note="color: #c95d72"
                     /translation="*IAN*NLTAYVTETIQTPHVDTERIDAYLLP*KRTIIQLGT*CVR
                     PSSTPISVGPVQELEEHPPL"
     polyA_signal    2114..2338
                     /label=bGH poly(A) signal
                     /note="color: #b4abac"
                     /note="color: #b4abac"
     rep_origin      2384..2812
                     /label=f1 ori
                     /note="color: #ffef86"
                     /note="color: #ffef86"
     promoter        2826..3155
                     /label=SV40 promoter
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     rep_origin      3006..3141
                     /label=SV40 ori
                     /note="color: #ffef86"
                     /note="color: #ffef86"
     promoter        3203..3250
                     /label=EM7 promoter
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     CDS             3269..3643
                     /label=BleoR
                     /note="color: #b7e6d7; direction: RIGHT"
                     /note="color: #------; direction: RIGHT"
     polyA_signal    3773..3894
                     /label=SV40 poly(A) signal
                     /note="color: #b4abac"
                     /note="color: #b4abac"
     primer_bind     complement(3943..3959)
                     /label=M13 rev
                     /note="color: #a020f0; direction: LEFT"
                     /note="color: #------; direction: LEFT"
     protein_bind    3967..3983
                     /label=lac operator
                     /note="color: #31849b"
                     /note="color: #31849b"
     promoter        complement(3991..4021)
                     /label=lac promoter
                     /note="color: #c6c9d1; direction: LEFT"
                     /note="color: #------; direction: LEFT"
     protein_bind    4036..4057
                     /label=CAP binding site
                     /note="color: #31849b"
                     /note="color: #31849b"
     rep_origin      complement(4345..4933)
                     /direction=LEFT
                     /label=ori
                     /note="color: #ffef86"
                     /note="color: #ffef86"
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
      841 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcc
      901 accatggccg aggtgcgtgc caaactggag gaacaggccc agcagatccg cctccaggct
      961 gaggccttcc aggccagact gaagagctgg tttgagcctc ttgtggagga tatgcagcgg
     1021 cagtgggccg ggctggtcga gaaggtgcag gccgccgtgg gcacttccgc cgcccctgtc
     1081 cccagcgaca accattgatg aattgccaat tgaaatttga cggcctatgt cactgaaact
     1141 atccaaacac cccatgtcga tactgaacgt atcgacgcat acctccttcc ttgaaaacgc
     1201 acaatcatac aactgggcac ataatgcgta cgcccatcta gtacacccat ctctgtaggt
     1261 ccagttcaag agctggaaga gcaccctcca cttacccgct gatcagcctc gactgtgcct
     1321 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     1381 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     1441 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     1501 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     1561 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     1621 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     1681 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc
     1741 atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     1801 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     1861 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     1921 tcggtctatt cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat
     1981 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     2041 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     2101 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     2161 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     2221 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     2281 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     2341 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     2401 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     2461 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     2521 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     2581 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     2641 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     2701 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     2761 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     2821 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     2881 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     2941 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     3001 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     3061 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     3121 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     3181 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     3241 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     3301 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3361 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3421 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3481 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3541 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3601 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3661 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3721 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3781 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3841 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3901 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3961 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     4021 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4081 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4141 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4201 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4261 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4321 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4381 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4441 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4501 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4561 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     4621 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4681 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4741 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4801 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4861 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     4921 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4981 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     5041 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     5101 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5161 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     5221 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     5281 aaaagtgcca cctgacgtcg
//
