LOCUS       .                       7229 bp    ds-DNA  circular SYN 30-OCT-2019
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 30, 2019 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     enhancer        234..613
                     /label="CMV enhancer"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     promoter        614..817
                     /label="CMV promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     Msc             862..879
                     /label="T7 promoter"
                     /note="color: #FF0000"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
     CDS             904..3222
                     /label="pST_H2FLK5_CAEEL_30-800"
                     /note="color: #FF0000"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
                     /translation="MKKLVDEVIPVAQGQVGNQHFDEPKSQKTPPSSLNDSYAEELLEA
                     KLINTDESSNTTHNKMSFMDKVKDTANSAYESVENTAVGAKDGVVGAFNTVGEKIGGAY
                     DSTKQYASDKVSAAGDYASEAGKKVGDAEDSMIQGAYDAKDSAKESASNAGNKISNAFT
                     SLKDSITGDNAHKTGEALNDNYESVKESASEAADRLKEPAQNTADNAKDLANQTGEEMS
                     DAFESVKQLTSDTAENAKENAHDAADTAKSYKDKAGEKASGILDSFKAHASDAKDSVEN
                     KASDAYNSAKDKAGDAWDSTKDAAGDAKDKTKSFTEKVGDKISGAYDSVKEKASDVADS
                     FKAHSTDSKDNVENKAADAYNTAKDKASDAWDKTKDKAGEAKDKMGDAWDTTKDKAGDA
                     WDTTKDKAGDGKGKAGDAWDTTKDKASDAWDTTKDKAGEAKDKMGEAWDHTKDKAGEAK
                     DKASDAADDAQGKSKSMTEKIGDSISGAWESTKDTAVSAKDKTAEAAGKVGDSISGAYD
                     TVKEKASDIADSFKAHSTDSKDNVENKAADAYNSAKDKASDAWDKTKDKAGDAKDKAAD
                     AWDTTKDKAGDAWDSTKDHAADAKDKASDAAGDAKDKSKSLTEKAGDAISGAYDSVKEK
                     ASDIADSFKAHSTNSKDNVENKASDAYNSAKDKASDAWDKTKDKAGEAKDKAGDAWDNT
                     KDKAGNAWDSTKDKASDAWDTTKDKASDAKESAGDAADSAKDKSKSITETIGDKISGAY
                     DSVKEKASDVADSFKAHSS*"
     polyA_signal    3242..3466
                     /label="bGH poly(A) signal"
                     /note="color: #b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
     rep_origin      3512..3940
                     /label="f1 ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     promoter        3954..4283
                     /label="SV40 promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     rep_origin      4134..4269
                     /label="SV40 ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     promoter        4331..4378
                     /label="EM7 promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     CDS             4397..4771
                     /label="BleoR"
                     /note="color: #b7e6d7; direction: RIGHT"
                     /ApEinfo_fwdcolor="#b7e6d7; direction: RIGHT"
                     /ApEinfo_revcolor="#b7e6d7; direction: RIGHT"
     polyA_signal    4901..5022
                     /label="SV40 poly(A) signal"
                     /note="color: #b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
     primer_bind     complement(5071..5087)
                     /label="M13 rev"
                     /note="color: #a020f0; direction: LEFT"
                     /ApEinfo_fwdcolor="#a020f0; direction: LEFT"
                     /ApEinfo_revcolor="#a020f0; direction: LEFT"
     protein_bind    5095..5111
                     /label="lac operator"
                     /note="color: #31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /ApEinfo_revcolor="#31849b"
     promoter        complement(5119..5149)
                     /label="lac promoter"
                     /note="color: #c6c9d1; direction: LEFT"
                     /ApEinfo_fwdcolor="#c6c9d1; direction: LEFT"
                     /ApEinfo_revcolor="#c6c9d1; direction: LEFT"
     protein_bind    5164..5185
                     /label="CAP binding site"
                     /note="color: #31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /ApEinfo_revcolor="#31849b"
     rep_origin      complement(5473..6061)
                     /direction=LEFT
                     /label="ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     CDS             complement(6232..7092)
                     /label="AmpR"
                     /note="color: #b7e6d7; direction: LEFT"
                     /ApEinfo_fwdcolor="#b7e6d7; direction: LEFT"
                     /ApEinfo_revcolor="#b7e6d7; direction: LEFT"
     promoter        complement(7093..7197)
                     /label="AmpR promoter"
                     /note="color: #c6c9d1; direction: LEFT"
                     /ApEinfo_fwdcolor="#c6c9d1; direction: LEFT"
                     /ApEinfo_revcolor="#c6c9d1; direction: LEFT"
     source          1..7229
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
      841 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcc
      901 accatgaaga agttggtgga tgaggtgata cccgtggcac agggacaggt tgggaaccag
      961 cattttgacg agcctaagtc ccagaaaacc ccgccatcta gcttgaatga cagctacgca
     1021 gaggagctgc tcgaggcaaa attgattaac accgacgaat ccagcaacac tacccacaat
     1081 aagatgagct ttatggataa ggtcaaggac actgccaact ctgcttacga gtccgtagaa
     1141 aatacagccg ttggggcaaa ggacggggta gtgggtgcct tcaacactgt gggtgagaaa
     1201 attggcggcg cctacgattc tacaaaacaa tatgcttctg ataaagtgtc tgccgccggt
     1261 gactatgctt cagaggcagg aaagaaggtc ggagacgccg aagacagcat gatacagggc
     1321 gcgtacgacg caaaggatag cgctaaagaa agcgcctcta atgccgggaa caagatttct
     1381 aacgcattca cctctctgaa ggactcaatc accggcgaca acgcccacaa gactggggaa
     1441 gccctgaatg acaattacga aagtgtcaag gagagcgcgt cagaggctgc cgatagactt
     1501 aaagaaccag cacagaacac ggcagacaac gccaaggact tggcaaacca gaccggcgag
     1561 gagatgtccg atgcatttga gagcgtcaag cagctgacaa gtgatacagc tgagaacgcc
     1621 aaggaaaacg cccacgacgc ggccgacacc gccaaatctt acaaagacaa agccggggag
     1681 aaagcctctg ggatacttga ctctttcaaa gcacacgctt ccgacgcaaa ggactctgtc
     1741 gaaaacaagg catcagacgc atataactcc gccaaggata aggccggtga cgcctgggat
     1801 tctacgaaag acgctgcggg cgacgcaaaa gacaagacca aatccttcac agaaaaggtc
     1861 ggggataaaa ttagcggagc ctatgacagt gtcaaagaga aagcgagtga cgtggccgac
     1921 agcttcaagg ctcatagcac ggacagcaag gacaacgtgg agaataaggc agccgacgca
     1981 tataacaccg ccaaggataa ggcctcagac gcatgggaca aaacaaagga taaggcaggc
     2041 gaggcgaagg ataagatggg tgacgcatgg gacaccacca aagataaagc aggggacgcc
     2101 tgggacacta ccaaggacaa ggccggagac ggaaagggta aggcaggcga cgcctgggac
     2161 acgaccaaag ataaagcatc cgacgcctgg gacaccacga aggacaaggc cggtgaggct
     2221 aaagataaga tgggcgaggc ttgggaccat accaaggata aagcaggcga ggctaaggac
     2281 aaggcttccg acgctgcaga tgacgctcag ggcaaaagta agagcatgac cgaaaagatt
     2341 ggtgattcaa tctctggcgc ttgggagagt accaaagata ccgcagtcag cgctaaggac
     2401 aagaccgctg aggctgcagg aaaagtcggt gatagtattt ccggcgctta cgacaccgtc
     2461 aaggagaaag cttctgatat cgccgactct ttcaaggccc acagtaccga ctccaaagac
     2521 aacgttgaga acaaggccgc ggacgcctac aactctgcca aggacaaagc cagtgacgcg
     2581 tgggacaaaa caaaagacaa ggctggcgac gctaaagata aagctgcaga cgcctgggac
     2641 acaactaagg acaaagccgg tgacgcctgg gactccacga aagaccacgc agctgacgca
     2701 aaagacaagg ccagcgatgc agccggggac gcgaaagata aatctaagtc cctcactgaa
     2761 aaggcgggag acgcaatatc aggcgcatac gactctgtga aggagaaagc atctgacatc
     2821 gctgactcat ttaaggccca ctccaccaac agcaaagaca acgtggaaaa caaagcaagc
     2881 gacgcgtaca actccgccaa ggacaaggca tccgacgctt gggacaaaac gaaagataag
     2941 gccggtgagg ctaaagacaa agctggcgac gcttgggata ataccaagga taaagctggg
     3001 aacgcatggg actcaacaaa ggataaagca tccgacgcct gggacaccac aaaagataag
     3061 gcgtcagacg ccaaagagtc tgcgggcgac gctgcagaca gtgccaaaga caagagtaaa
     3121 agcattacgg agaccatcgg cgacaagata agcggagcct acgattccgt caaggaaaag
     3181 gcttccgacg tggccgattc ctttaaagcg catagctctt agacccgctg atcagcctcg
     3241 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3301 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3361 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     3421 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     3481 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     3541 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     3601 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     3661 aatcggggca tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     3721 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     3781 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     3841 aaccctatct cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg
     3901 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     3961 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4021 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4081 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4141 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4201 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     4261 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     4321 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     4381 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     4441 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     4501 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     4561 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     4621 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     4681 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     4741 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
     4801 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     4861 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     4921 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     4981 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     5041 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     5101 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     5161 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     5221 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     5281 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     5341 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     5401 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     5461 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5521 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5581 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     5641 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     5701 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     5761 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     5821 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     5881 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     5941 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6001 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6061 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6121 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6181 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     6241 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     6301 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     6361 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     6421 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     6481 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     6541 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     6601 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     6661 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     6721 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     6781 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     6841 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     6901 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     6961 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7021 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7081 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7141 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     7201 atttccccga aaagtgccac ctgacgtcg
//
