LOCUS       Exported                6635 bp DNA     circular SYN 06-APR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 6, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6635
                     /mol_type="other DNA"
                     /note="/dnas_title=."
                     /note="color: #ffffff"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     source          904..2628
                     /label=/dnas_title=Annotated insert
                     /note="/dnas_title=Annotated insert"
                     /note="color: #ffffff"
                     /note="color: #ffffff"
                     /organism="unspecified"
     enhancer        234..613
                     /label=CMV enhancer
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     promoter        614..817
                     /label=CMV promoter
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     Msc             862..879
                     /label=T7 promoter
                     /note="color: #ff0000"
                     /note="color: #ff0000"
     CDS             904..906
                     /codon_start=1
                     /label=Start
                     /note="Start"
                     /note="color: #008000"
                     /note="color: #ff0000"
                     /translation="M"
     CDS             907..1548
                     /codon_start=1
                     /label=FUSN
                     /note="FUSN"
                     /note="color: #ccffcc"
                     /note="color: #ff9900"
                     /translation="ASNDYTQQATQSYGAYPTQPGQGYSQQSSQPYGQQSYSGYSQSTD
                     TSGYGQSSYSSYGQSQNTGYGTQSTPQGYGSTGGYGSSQSSQSSYGQQSSYPGYGQQPA
                     PSSTSGSYGSSSQSSSYGQPQSGSYSQQPSYGGQQQSYGQQQSYNPPQGYGQQNQYNSS
                     SGGGGGGGGGGNYGQDQSSMSSGGGSGGGYGNQDQSGGGGSGGYGQQDRGG"
     CDS             1549..1554
                     /codon_start=1
                     /label=GG linker
                     /note="GG linker"
                     /note="color: #800080"
                     /note="color: #808080"
                     /translation="GG"
     CDS             1555..2265
                     /codon_start=1
                     /label=Dan's mChery
                     /note="Dan's mChery"
                     /note="color: #993366"
                     /note="color: #ff00ff"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKA"
     CDS             2266..2298
                     /codon_start=1
                     /label=Dan's mCherry to SspB linker
                     /note="Dan's mCherry to SspB linker"
                     /note="color: #ff00ff"
                     /note="color: #999999"
                     /translation="AGSGGTSGGSS"
     CDS             2299..2625
                     /codon_start=1
                     /label=Dan's SspB
                     /note="Dan's SspB"
                     /note="color: #00ff00"
                     /note="color: #00ff00"
                     /translation="PKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQIV
                     LNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEIYD
                     ELNIG"
     CDS             2626..2628
                     /codon_start=1
                     /label=Stop codon
                     /note="Stop codon"
                     /note="color: #cc99ff"
                     /note="color: #800000"
                     /translation=""
     polyA_signal    2648..2872
                     /label=bGH poly(A) signal
                     /note="color: #b4abac"
                     /note="color: #b4abac"
     rep_origin      2918..3346
                     /label=f1 ori
                     /note="color: #ffef86"
                     /note="color: #ffef86"
     promoter        3360..3689
                     /label=SV40 promoter
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     rep_origin      3540..3675
                     /label=SV40 ori
                     /note="color: #ffef86"
                     /note="color: #ffef86"
     promoter        3737..3784
                     /label=EM7 promoter
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     CDS             3803..4177
                     /label=BleoR
                     /note="color: #b7e6d7; direction: RIGHT"
                     /note="color: #------; direction: RIGHT"
     polyA_signal    4307..4428
                     /label=SV40 poly(A) signal
                     /note="color: #b4abac"
                     /note="color: #b4abac"
     primer_bind     complement(4477..4493)
                     /label=M13 rev
                     /note="color: #a020f0; direction: LEFT"
                     /note="color: #------; direction: LEFT"
     protein_bind    4501..4517
                     /label=lac operator
                     /note="color: #31849b"
                     /note="color: #31849b"
     promoter        complement(4525..4555)
                     /label=lac promoter
                     /note="color: #c6c9d1; direction: LEFT"
                     /note="color: #------; direction: LEFT"
     protein_bind    4570..4591
                     /label=CAP binding site
                     /note="color: #31849b"
                     /note="color: #31849b"
     rep_origin      complement(4879..5467)
                     /direction=LEFT
                     /label=ori
                     /note="color: #ffef86"
                     /note="color: #ffef86"
     CDS             complement(5638..6498)
                     /label=AmpR
                     /note="color: #b7e6d7; direction: LEFT"
                     /note="color: #------; direction: LEFT"
     promoter        complement(6499..6603)
                     /label=AmpR promoter
                     /note="color: #c6c9d1; direction: LEFT"
                     /note="color: #------; direction: LEFT"
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
      841 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcc
      901 accatggcct caaacgatta tacccaacaa gcaacccaaa gctatggggc ctaccccacc
      961 cagcccgggc agggctattc ccagcagagc agtcagccct acggacagca gagttacagt
     1021 ggttatagcc agtccacgga cacttcaggc tatggccaga gcagctattc ttcttatggc
     1081 cagagccaga acacaggcta tggaactcag tcaactcccc agggatatgg ctcgactggc
     1141 ggctatggca gtagccagag ctcccaatcg tcttacgggc agcagtcctc ctatcctggc
     1201 tatggccagc agccagctcc cagcagcacc tcgggaagtt acggtagcag ttctcagagc
     1261 agcagctatg ggcagcccca gagtgggagc tacagccagc agcctagcta tggtggacag
     1321 cagcaaagct atggacagca gcaaagctat aatccccctc agggctatgg acagcagaac
     1381 cagtacaaca gcagcagtgg tggtggaggc ggaggcggag gtggaggtaa ctatggccaa
     1441 gatcaatcct ccatgagtag tggtggtggc agtggtggcg gttatggcaa tcaagaccag
     1501 agtggtggag gtggcagcgg tggctatgga cagcaggacc gtggaggtgg aggaatggtg
     1561 tctaaaggcg aggaggataa catggctata atcaaggaat ttatgaggtt caaggtgcat
     1621 atggagggat ctgtgaacgg tcacgagttc gaaatcgagg gcgaaggcga ggggcgaccc
     1681 tatgaaggga cacagacggc caaactgaag gtgaccaagg gtggccccct gcctttcgcc
     1741 tgggacatcc tgtcacccca gtttatgtac ggctctaagg cttacgttaa gcaccctgcc
     1801 gatattccgg actacttgaa actgagcttc cctgaagggt tcaagtggga acgggtaatg
     1861 aattttgagg atggcggtgt agtcacagtt acccaggaca gctctcttca ggacggagaa
     1921 tttatctata aggttaaact tcggggcact aacttcccat cagacggccc cgtgatgcag
     1981 aagaaaacta tgggctggga agccagctca gaacgcatgt atcccgagga cggagcactg
     2041 aagggagaaa tcaagcagcg actcaagctg aaggatgggg gacattatga tgctgaggtc
     2101 aaaaccacct ataaggccaa gaaacccgtt cagcttcctg gggcatacaa cgtgaatatc
     2161 aaactggata ttacatccca taacgaagac tataccatcg tggaacagta cgagcgggcc
     2221 gagggcagac atagcacagg gggcatggat gaattgtaca aggcggccgg atccggtggt
     2281 acctctggag gtagctcccc gaaacgccct aagctgctgc gtgaatatta cgattggctg
     2341 gttgataaca gctttacccc atatctggtg gtggatgcca catacctggg cgtgaacgtg
     2401 cccgtggagt atgtgaaaga cggtcagatc gtgctgaatc tgtctgcaag tgcgaccggc
     2461 aacctgcaac tgacaaatga ttttatccag ttcaacgccc gctttaaggg cgtgtctcgt
     2521 gaactgtata tcccgatggg tgccgctctg gccatttacg ctcgcgagaa cggcgatggt
     2581 gtgatgttcg aaccagaaga aatctatgac gagctgaata ttggttaaac ccgctgatca
     2641 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     2701 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     2761 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     2821 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     2881 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     2941 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     3001 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3061 gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     3121 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     3181 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     3241 acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc
     3301 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     3361 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     3421 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     3481 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     3541 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     3601 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     3661 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     3721 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     3781 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     3841 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     3901 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     3961 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     4021 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     4081 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     4141 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     4201 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     4261 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     4321 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     4381 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     4441 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     4501 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     4561 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     4621 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     4681 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4741 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4801 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4861 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4921 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4981 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     5041 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     5101 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     5161 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     5221 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     5281 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     5341 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     5401 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     5461 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     5521 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     5581 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     5641 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     5701 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     5761 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     5821 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     5881 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     5941 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     6001 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     6061 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     6121 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     6181 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     6241 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     6301 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     6361 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     6421 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     6481 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     6541 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     6601 gcgcacattt ccccgaaaag tgccacctga cgtcg
//
