LOCUS       Exported                6716 bp DNA     circular SYN 06-APR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 6, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6716
                     /mol_type="other DNA"
                     /note="/dnas_title=."
                     /note="color: #ffffff"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     enhancer        234..613
                     /label=CMV enhancer
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     promoter        614..817
                     /label=CMV promoter
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     Msc             862..879
                     /label=T7 promoter
                     /note="color: #ff0000"
                     /note="color: #ff0000"
     CDS             904..906
                     /codon_start=1
                     /label=Start
                     /note="color: #ff0000"
                     /translation="M"
     CDS             907..930
                     /codon_start=1
                     /label=FLAG tag
                     /note="FLAG tag"
                     /note="color: #800000"
                     /translation="DYKDDDDK"
     CDS             931..1611
                     /codon_start=1
                     /label=Dan's CAHS2_Parrc
                     /note="color: #5eb4de"
                     /note="color: #5eb4de"
                     /translation="MEAMNMNIPRDAMFVPPPESEQNGYHEKSEVQQTSYMQSQVKVPH
                     YNFPTPYFTTSFSAQELLGEGFQASISRISAVTEDMQSMEIPEFVEEARRDYAAKTREN
                     EMLGQQYEKELERKSEAYRKHQEVEADKIRKELEKQHMRDIEFRKEIAELAIENQKRMI
                     DLECRYAKKDMDRERTKVRMMLEQQKFHSDIQVNLDSSAAGTESGGHVVSQSEKFTERN
                     REMKR"
     CDS             1612..1635
                     /codon_start=1
                     /label=Dan's SGGSGGSGG linker
                     /note="color: #c9674e"
                     /note="color: #c9674e"
                     /translation="SGGGGSGG"
     CDS             1636..2346
                     /codon_start=1
                     /label=Dan's mCherry
                     /note="color: #9a36cc"
                     /note="color: #9a36cc"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKA"
     CDS             2347..2379
                     /codon_start=1
                     /label=Dan's mCherry to SspB linker
                     /note="color: #c7949c"
                     /note="color: #c7949c"
                     /translation="AGSGGTSGGSS"
     CDS             2380..2706
                     /codon_start=1
                     /label=Dan's SspB
                     /note="color: #245122"
                     /note="color: #245122"
                     /translation="PKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQIV
                     LNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEIYD
                     ELNIG"
     CDS             2707..2709
                     /codon_start=1
                     /label=Stop
                     /note="Stop"
                     /note="color: #3366ff"
                     /translation=""
     polyA_signal    2729..2953
                     /label=bGH poly(A) signal
                     /note="color: #b4abac"
                     /note="color: #b4abac"
     rep_origin      2999..3427
                     /label=f1 ori
                     /note="color: #ffef86"
                     /note="color: #ffef86"
     promoter        3441..3770
                     /label=SV40 promoter
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     rep_origin      3621..3756
                     /label=SV40 ori
                     /note="color: #ffef86"
                     /note="color: #ffef86"
     promoter        3818..3865
                     /label=EM7 promoter
                     /note="color: #c6c9d1"
                     /note="color: #c6c9d1"
     CDS             3884..4258
                     /label=BleoR
                     /note="color: #b7e6d7; direction: RIGHT"
                     /note="color: #------; direction: RIGHT"
     polyA_signal    4388..4509
                     /label=SV40 poly(A) signal
                     /note="color: #b4abac"
                     /note="color: #b4abac"
     primer_bind     complement(4558..4574)
                     /label=M13 rev
                     /note="color: #a020f0; direction: LEFT"
                     /note="color: #------; direction: LEFT"
     protein_bind    4582..4598
                     /label=lac operator
                     /note="color: #31849b"
                     /note="color: #31849b"
     promoter        complement(4606..4636)
                     /label=lac promoter
                     /note="color: #c6c9d1; direction: LEFT"
                     /note="color: #------; direction: LEFT"
     protein_bind    4651..4672
                     /label=CAP binding site
                     /note="color: #31849b"
                     /note="color: #31849b"
     rep_origin      complement(4960..5548)
                     /direction=LEFT
                     /label=ori
                     /note="color: #ffef86"
                     /note="color: #ffef86"
     CDS             complement(5719..6579)
                     /label=AmpR
                     /note="color: #b7e6d7; direction: LEFT"
                     /note="color: #------; direction: LEFT"
     promoter        complement(6580..6684)
                     /label=AmpR promoter
                     /note="color: #c6c9d1; direction: LEFT"
                     /note="color: #------; direction: LEFT"
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
      841 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcc
      901 accatggatt acaaggatga tgatgataag atggaggcca tgaatatgaa tatcccccgc
      961 gatgccatgt ttgttccgcc accggaatct gagcaaaatg ggtatcatga gaagtcagaa
     1021 gttcagcaaa caagttatat gcagagtcaa gtcaaagtgc cacattataa tttcccgaca
     1081 ccatatttta cgacttcctt ttctgcgcaa gagctgctgg gcgaagggtt tcaagcctca
     1141 atttcccgta tttcagccgt tacggaagac atgcagagca tggaaatccc ggagttcgtt
     1201 gaagaggccc gccgtgatta cgcagccaaa acacgtgaaa atgagatgct ggggcaacaa
     1261 tatgaaaaag agctggaacg taagtccgaa gcctaccgca aacatcagga agtagaggcc
     1321 gacaaaatcc gcaaagaact tgaaaaacag catatgcgtg atattgaatt tcggaaagaa
     1381 attgcagaac tggcgattga gaaccaaaaa cgtatgatcg atcttgaatg ccgctatgca
     1441 aaaaaagaca tggaccggga acgcacaaaa gttcgtatga tgctggagca acagaaattc
     1501 catagtgata tccaggtaaa tctggattct tctgcggctg ggaccgagag cggaggtcat
     1561 gtagtgagcc agtctgaaaa gttcaccgaa cgtaaccgcg agatgaaacg ctccggaggc
     1621 ggcggatccg gcggaatggt gtctaaaggc gaggaggata acatggctat aatcaaggaa
     1681 tttatgaggt tcaaggtgca tatggaggga tctgtgaacg gtcacgagtt cgaaatcgag
     1741 ggcgaaggcg aggggcgacc ctatgaaggg acacagacgg ccaaactgaa ggtgaccaag
     1801 ggtggccccc tgcctttcgc ctgggacatc ctgtcacccc agtttatgta cggctctaag
     1861 gcttacgtta agcaccctgc cgatattccg gactacttga aactgagctt ccctgaaggg
     1921 ttcaagtggg aacgggtaat gaattttgag gatggcggtg tagtcacagt tacccaggac
     1981 agctctcttc aggacggaga atttatctat aaggttaaac ttcggggcac taacttccca
     2041 tcagacggcc ccgtgatgca gaagaaaact atgggctggg aagccagctc agaacgcatg
     2101 tatcccgagg acggagcact gaagggagaa atcaagcagc gactcaagct gaaggatggg
     2161 ggacattatg atgctgaggt caaaaccacc tataaggcca agaaacccgt tcagcttcct
     2221 ggggcataca acgtgaatat caaactggat attacatccc ataacgaaga ctataccatc
     2281 gtggaacagt acgagcgggc cgagggcaga catagcacag ggggcatgga tgaattgtac
     2341 aaggcggccg gatccggtgg tacctctgga ggtagctccc cgaaacgccc taagctgctg
     2401 cgtgaatatt acgattggct ggttgataac agctttaccc catatctggt ggtggatgcc
     2461 acatacctgg gcgtgaacgt gcccgtggag tatgtgaaag acggtcagat cgtgctgaat
     2521 ctgtctgcaa gtgcgaccgg caacctgcaa ctgacaaatg attttatcca gttcaacgcc
     2581 cgctttaagg gcgtgtctcg tgaactgtat atcccgatgg gtgccgctct ggccatttac
     2641 gctcgcgaga acggcgatgg tgtgatgttc gaaccagaag aaatctatga cgagctgaat
     2701 attggttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     2761 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     2821 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     2881 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     2941 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     3001 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3061 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3121 ttcgccggct ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt
     3181 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     3241 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     3301 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     3361 gggattttgg ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     3421 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     3481 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     3541 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     3601 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     3661 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     3721 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     3781 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     3841 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
     3901 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     3961 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     4021 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     4081 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     4141 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     4201 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     4261 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     4321 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     4381 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4441 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4501 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     4561 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     4621 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     4681 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     4741 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     4801 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4861 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4921 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4981 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     5041 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     5101 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     5161 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     5221 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     5281 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     5341 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     5401 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     5461 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5521 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     5581 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5641 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5701 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5761 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5821 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5881 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5941 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6001 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     6061 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     6121 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     6181 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     6241 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     6301 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     6361 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6421 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6481 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6541 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6601 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     6661 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcg
//
