LOCUS       .                       6161 bp    ds-DNA  circular SYN 30-OCT-2019
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 30, 2019 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     enhancer        234..613
                     /label="CMV enhancer"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     promoter        614..817
                     /label="CMV promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     Msc             862..879
                     /label="T7 promoter"
                     /note="color: #FF0000"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
     CDS             904..906
                     /label="Start"
                     /note="color: #0a6530"
                     /ApEinfo_fwdcolor="#0a6530"
                     /ApEinfo_revcolor="#0a6530"
                     /translation="M"
     CDS             907..1056
                     /label="PvLEA4_repeats_k3_26"
                     /note="color: #e90064"
                     /ApEinfo_fwdcolor="#e90064"
                     /ApEinfo_revcolor="#e90064"
                     /translation="FQTAADKLAAAKDTVVDTLGSAKDTVVEKVVGAKDATAEYLGEKK
                     EQMRD"
     CDS             1057..1080
                     /label="Dan's SGGSGGSGG linker"
                     /note="color: #866662"
                     /ApEinfo_fwdcolor="#866662"
                     /ApEinfo_revcolor="#866662"
                     /translation="SGGGGSGG"
     CDS             1081..1791
                     /label="Dan's mCherry"
                     /note="color: #8c328d"
                     /ApEinfo_fwdcolor="#8c328d"
                     /ApEinfo_revcolor="#8c328d"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKA"
     CDS             1792..1824
                     /label="Dan's mCherry to SspB linker"
                     /note="color: #f140a7"
                     /ApEinfo_fwdcolor="#f140a7"
                     /ApEinfo_revcolor="#f140a7"
                     /translation="AGSGGTSGGSS"
     CDS             1825..2151
                     /label="Dan's SspB"
                     /note="color: #d1fb35"
                     /ApEinfo_fwdcolor="#d1fb35"
                     /ApEinfo_revcolor="#d1fb35"
                     /translation="PKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQIV
                     LNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEIYD
                     ELNIG"
     CDS             2152..2154
                     /label="stop ochre TAA"
                     /note="color: #b602d4"
                     /ApEinfo_fwdcolor="#b602d4"
                     /ApEinfo_revcolor="#b602d4"
                     /translation="*"
     polyA_signal    2174..2398
                     /label="bGH poly(A) signal"
                     /note="color: #b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
     rep_origin      2444..2872
                     /label="f1 ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     promoter        2886..3215
                     /label="SV40 promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     rep_origin      3066..3201
                     /label="SV40 ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     promoter        3263..3310
                     /label="EM7 promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     CDS             3329..3703
                     /label="BleoR"
                     /note="color: #b7e6d7; direction: RIGHT"
                     /ApEinfo_fwdcolor="#b7e6d7; direction: RIGHT"
                     /ApEinfo_revcolor="#b7e6d7; direction: RIGHT"
     polyA_signal    3833..3954
                     /label="SV40 poly(A) signal"
                     /note="color: #b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
     primer_bind     complement(4003..4019)
                     /label="M13 rev"
                     /note="color: #a020f0; direction: LEFT"
                     /ApEinfo_fwdcolor="#a020f0; direction: LEFT"
                     /ApEinfo_revcolor="#a020f0; direction: LEFT"
     protein_bind    4027..4043
                     /label="lac operator"
                     /note="color: #31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /ApEinfo_revcolor="#31849b"
     promoter        complement(4051..4081)
                     /label="lac promoter"
                     /note="color: #c6c9d1; direction: LEFT"
                     /ApEinfo_fwdcolor="#c6c9d1; direction: LEFT"
                     /ApEinfo_revcolor="#c6c9d1; direction: LEFT"
     protein_bind    4096..4117
                     /label="CAP binding site"
                     /note="color: #31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /ApEinfo_revcolor="#31849b"
     rep_origin      complement(4405..4993)
                     /direction=LEFT
                     /label="ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     CDS             complement(5164..6024)
                     /label="AmpR"
                     /note="color: #b7e6d7; direction: LEFT"
                     /ApEinfo_fwdcolor="#b7e6d7; direction: LEFT"
                     /ApEinfo_revcolor="#b7e6d7; direction: LEFT"
     promoter        complement(6025..6129)
                     /label="AmpR promoter"
                     /note="color: #c6c9d1; direction: LEFT"
                     /ApEinfo_fwdcolor="#c6c9d1; direction: LEFT"
                     /ApEinfo_revcolor="#c6c9d1; direction: LEFT"
     source          1..6161
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
      841 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcc
      901 accatgttcc agaccgccgc cgacaagctg gccgccgcca aggacaccgt ggttgacacc
      961 ttgggcagcg ccaaggacac cgtggtggag aaggtggtgg gcgccaagga cgccaccgcc
     1021 gagtacctgg gagagaagaa ggagcagatg agggactccg gaggcggcgg atccggcgga
     1081 atggtgtcta aaggcgagga ggataacatg gctataatca aggaatttat gaggttcaag
     1141 gtgcatatgg agggatctgt gaacggtcac gagttcgaaa tcgagggcga aggcgagggg
     1201 cgaccctatg aagggacaca gacggccaaa ctgaaggtga ccaagggtgg ccccctgcct
     1261 ttcgcctggg acatcctgtc accccagttt atgtacggct ctaaggctta cgttaagcac
     1321 cctgccgata ttccggacta cttgaaactg agcttccctg aagggttcaa gtgggaacgg
     1381 gtaatgaatt ttgaggatgg cggtgtagtc acagttaccc aggacagctc tcttcaggac
     1441 ggagaattta tctataaggt taaacttcgg ggcactaact tcccatcaga cggccccgtg
     1501 atgcagaaga aaactatggg ctgggaagcc agctcagaac gcatgtatcc cgaggacgga
     1561 gcactgaagg gagaaatcaa gcagcgactc aagctgaagg atgggggaca ttatgatgct
     1621 gaggtcaaaa ccacctataa ggccaagaaa cccgttcagc ttcctggggc atacaacgtg
     1681 aatatcaaac tggatattac atcccataac gaagactata ccatcgtgga acagtacgag
     1741 cgggccgagg gcagacatag cacagggggc atggatgaat tgtacaaggc ggccggatcc
     1801 ggtggtacct ctggaggtag ctccccgaaa cgccctaagc tgctgcgtga atattacgat
     1861 tggctggttg ataacagctt taccccatat ctggtggtgg atgccacata cctgggcgtg
     1921 aacgtgcccg tggagtatgt gaaagacggt cagatcgtgc tgaatctgtc tgcaagtgcg
     1981 accggcaacc tgcaactgac aaatgatttt atccagttca acgcccgctt taagggcgtg
     2041 tctcgtgaac tgtatatccc gatgggtgcc gctctggcca tttacgctcg cgagaacggc
     2101 gatggtgtga tgttcgaacc agaagaaatc tatgacgagc tgaatattgg ttaaacccgc
     2161 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     2221 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     2281 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     2341 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     2401 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     2461 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     2521 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     2581 cgtcaagctc taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc
     2641 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     2701 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     2761 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt
     2821 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     2881 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     2941 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     3001 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     3061 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     3121 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     3181 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     3241 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
     3301 ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct
     3361 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
     3421 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
     3481 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
     3541 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
     3601 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
     3661 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt
     3721 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     3781 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     3841 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     3901 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     3961 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     4021 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     4081 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     4141 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     4201 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     4261 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     4321 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     4381 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     4441 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     4501 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4561 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
     4621 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4681 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4741 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4801 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     4861 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4921 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4981 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5041 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5101 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     5161 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     5221 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     5281 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     5341 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     5401 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     5461 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     5521 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     5581 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     5641 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     5701 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     5761 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     5821 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     5881 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     5941 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     6001 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     6061 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     6121 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc g
//
