LOCUS       .                       6856 bp    DNA     circular SYN 22-JUN-2021
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 22, 2021 from SnapGene Viewer 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     promoter        311..614
                     /label="CMV enhancer"
                     /note="color: #c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /ApEinfo_revcolor="#c7b0e3"
     misc_feature    820..861
                     /label="teto2x2"
                     /note="color: #f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
     misc_feature    997..1007
                     /label="Geneious type: Editing History Insertion"
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
     misc_feature    997..1000
                     /label="Ligation"
                     /label="nonstandard type: ligation"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
     CDS             1008..1010
                     /label="Start"
                     /note="color: #992b2b"
                     /ApEinfo_fwdcolor="#992b2b"
                     /ApEinfo_revcolor="#992b2b"
                     /translation="M"
     CDS             1011..1397
                     /label="DHN_VrDHN1a"
                     /note="color: #bcc590"
                     /ApEinfo_fwdcolor="#bcc590"
                     /ApEinfo_revcolor="#bcc590"
                     /translation="AYQQDPCANPTRQTGKTGGQTDQYGNPVHQTEALGAYGAGTGTGM
                     HGGEHQQQPHQQPGVLNRSGSSSSEDDGQGGRRKKGMKEKIKERIPGMGRKDEQKQTSA
                     TSTPGQGQQQKGMMEKIKEKLPGAH"
     CDS             1398..1421
                     /label="Dan's SGGSGGSGG linker"
                     /note="color: #9e2273"
                     /ApEinfo_fwdcolor="#9e2273"
                     /ApEinfo_revcolor="#9e2273"
                     /translation="SGGGGSGG"
     CDS             1422..2132
                     /label="Dan's mCherry"
                     /note="color: #877fec"
                     /ApEinfo_fwdcolor="#877fec"
                     /ApEinfo_revcolor="#877fec"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKA"
     source          2139..2459
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
     source          2460..2786
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
     source          2787..2822
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
     misc_feature    2133..2828
                     /label="Geneious type: Editing History Insertion"
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
     misc_feature    2133..2138
                     /label="RTgb1a N-FKBPF36V-HA (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:j-d0hi8c2"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
     CDS             2139..2459
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label="F36V mutant of FK506-binding protein FKBP12 CDS"
                     /note="DmrB"
                     /note="binds synthetic ligands that do not bind wild-type
                     FKBP"
                     /note="color: #993366"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
                     /ApEinfo_fwdcolor="#993366"
                     /ApEinfo_revcolor="#993366"
     CDS             2466..2786
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label="F36V mutant of FK506-binding protein FKBP12 CDS"
                     /note="DmrB"
                     /note="binds synthetic ligands that do not bind wild-type
                     FKBP"
                     /note="color: #993366"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
                     /ApEinfo_fwdcolor="#993366"
                     /ApEinfo_revcolor="#993366"
     CDS             2793..2819
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label="HA (human influenza hemagglutinin) epitope tag CDS"
                     /note="HA"
                     /note="color: #993366"
                     /translation="YPYDVPDYA"
                     /ApEinfo_fwdcolor="#993366"
                     /ApEinfo_revcolor="#993366"
     misc_feature    2825..2828
                     /label="Ligation"
                     /label="nonstandard type: ligation"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
     rep_origin      3160..3466
                     /label="F1 ori"
                     /note="color: #9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /ApEinfo_revcolor="#9eafd2"
     primer_bind     complement(4698..4718)
                     /label="M13-rev"
                     /note="color: #85dae9; direction: LEFT"
                     /ApEinfo_fwdcolor="#85dae9; direction: LEFT"
                     /ApEinfo_revcolor="#85dae9; direction: LEFT"
     misc_binding    complement(4724..4746)
                     /label="LacO"
                     /note="color: #84b0dc; direction: LEFT"
                     /ApEinfo_fwdcolor="#84b0dc; direction: LEFT"
                     /ApEinfo_revcolor="#84b0dc; direction: LEFT"
     promoter        complement(4750..4780)
                     /label="lac promoter"
                     /note="color: #c7b0e3; direction: LEFT"
                     /ApEinfo_fwdcolor="#c7b0e3; direction: LEFT"
                     /ApEinfo_revcolor="#c7b0e3; direction: LEFT"
     CDS             complement(5863..6522)
                     /label="AmpR"
                     /note="color: #ffef86; direction: LEFT"
                     /ApEinfo_fwdcolor="#ffef86; direction: LEFT"
                     /ApEinfo_revcolor="#ffef86; direction: LEFT"
     source          1..6856
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
     misc_feature    997..2828
                     /label="RTgb1a N-FKBPF36V-HA (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:j-d0hi8c2"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
     misc_feature    join(3677..7708,1..1000)
                     /label="rt1a pCDNA4-TO (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:o-d81dp1x"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggaacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cgccaccatg gcctaccagc
     1021 aggacccctg cgccaacccc accaggcaga ccggaaagac cggaggacag accgaccagt
     1081 acggcaaccc cgtgcaccag accgaggccc tgggagccta cggagctgga accggaaccg
     1141 gcatgcacgg aggagagcac cagcagcagc cccaccagca gcctggagtg ctgaacagga
     1201 gcggcagcag cagcagcgag gacgacggcc agggaggaag gaggaagaag ggcatgaagg
     1261 agaagatcaa ggagaggatc cccggcatgg gcaggaagga cgagcagaag cagaccagcg
     1321 ccaccagcac ccccggacag ggccaacagc agaagggcat gatggagaag atcaaggaga
     1381 agctgcccgg cgcccactcc ggaggcggcg gatccggcgg aatggtgtct aaaggcgagg
     1441 aggataacat ggctataatc aaggaattta tgaggttcaa ggtgcatatg gagggatctg
     1501 tgaacggtca cgagttcgaa atcgagggcg aaggcgaggg gcgaccctat gaagggacac
     1561 agacggccaa actgaaggtg accaagggtg gccccctgcc tttcgcctgg gacatcctgt
     1621 caccccagtt tatgtacggc tctaaggctt acgttaagca ccctgccgat attccggact
     1681 acttgaaact gagcttccct gaagggttca agtgggaacg ggtaatgaat tttgaggatg
     1741 gcggtgtagt cacagttacc caggacagct ctcttcagga cggagaattt atctataagg
     1801 ttaaacttcg gggcactaac ttcccatcag acggccccgt gatgcagaag aaaactatgg
     1861 gctgggaagc cagctcagaa cgcatgtatc ccgaggacgg agcactgaag ggagaaatca
     1921 agcagcgact caagctgaag gatgggggac attatgatgc tgaggtcaaa accacctata
     1981 aggccaagaa acccgttcag cttcctgggg catacaacgt gaatatcaaa ctggatatta
     2041 catcccataa cgaagactat accatcgtgg aacagtacga gcgggccgag ggcagacata
     2101 gcacaggggg catggatgaa ttgtacaagg cgactagagg cgtccaagtc gaaaccatta
     2161 gtcccggcga tggcagaaca tttcctaaaa ggggacaaac atgtgtcgtc cattatacag
     2221 gcatgttgga ggacggcaaa aaggtggaca gtagtagaga tcgcaataaa cctttcaaat
     2281 tcatgttggg aaaacaagaa gtcattaggg gatgggagga gggcgtggct caaatgtccg
     2341 tcggccaacg cgctaagctc accatcagcc ccgactacgc atacggcgct accggacatc
     2401 ccggaattat tccccctcac gctaccttgg tgtttgacgt cgaactgttg aagctcgaga
     2461 ctagaggagt gcaggtggag actatctccc caggagacgg gcgcaccttc cccaagcgcg
     2521 gccagacctg cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa gttgattcct
     2581 cccgggacag aaacaagccc tttaagttta tgctaggcaa gcaggaggtg atccgaggct
     2641 gggaagaagg ggttgcccag atgagtgtgg gtcagagagc caaactgact atatctccag
     2701 attatgccta tggtgccact gggcacccag gcatcatccc accacatgcc actctcgtct
     2761 tcgatgtgga gcttctaaaa ctggaaacta gttatccgta cgacgtacca gactacgcat
     2821 aagcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct
     2881 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     2941 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     3001 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     3061 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     3121 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     3181 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     3241 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     3301 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     3361 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     3421 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     3481 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     3541 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     3601 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3661 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3721 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     3781 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3841 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     3901 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     3961 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     4021 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     4081 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     4141 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     4201 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     4261 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     4321 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     4381 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     4441 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     4501 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     4561 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     4621 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     4681 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     4741 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     4801 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     4861 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4921 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4981 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     5041 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     5101 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     5161 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     5221 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     5281 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5341 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     5401 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     5461 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5521 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     5581 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5641 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5701 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5761 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5821 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5881 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5941 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     6001 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     6061 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     6121 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     6181 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     6241 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     6301 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     6361 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     6421 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     6481 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     6541 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     6601 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     6661 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6721 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6781 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6841 agtgccacct gacgtc
//
