LOCUS       .                       6619 bp    DNA     circular SYN 22-JUN-2021
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 22, 2021 from SnapGene Viewer 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     promoter        311..614
                     /label="CMV enhancer"
                     /note="color: #c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /ApEinfo_revcolor="#c7b0e3"
     misc_feature    820..861
                     /label="teto2x2"
                     /note="color: #f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
     misc_feature    997..1007
                     /label="Geneious type: Editing History Insertion"
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
     misc_feature    997..1000
                     /label="Ligation"
                     /label="nonstandard type: ligation"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
     CDS             1008..1010
                     /label="Start"
                     /note="color: #992b2b"
                     /ApEinfo_fwdcolor="#992b2b"
                     /ApEinfo_revcolor="#992b2b"
                     /translation="M"
     CDS             1011..1160
                     /label="PvLEA4_repeats_k5_1"
                     /note="color: #10a59e"
                     /ApEinfo_fwdcolor="#10a59e"
                     /ApEinfo_revcolor="#10a59e"
                     /translation="FQTAADKAAAAKDTVVDTLCDAKDTAKEKVVGAKDAGADYAGEKK
                     EQMRD"
     CDS             1161..1184
                     /label="Dan's SGGSGGSGG linker"
                     /note="color: #9e2273"
                     /ApEinfo_fwdcolor="#9e2273"
                     /ApEinfo_revcolor="#9e2273"
                     /translation="SGGGGSGG"
     CDS             1185..1895
                     /label="Dan's mCherry"
                     /note="color: #877fec"
                     /ApEinfo_fwdcolor="#877fec"
                     /ApEinfo_revcolor="#877fec"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKA"
     source          1902..2222
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
     source          2223..2549
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
     source          2550..2585
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
     misc_feature    1896..2591
                     /label="Geneious type: Editing History Insertion"
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
     misc_feature    1896..1901
                     /label="RTgb1a N-FKBPF36V-HA (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:j-d0hi8c2"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
     CDS             1902..2222
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label="F36V mutant of FK506-binding protein FKBP12 CDS"
                     /note="DmrB"
                     /note="binds synthetic ligands that do not bind wild-type
                     FKBP"
                     /note="color: #993366"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
                     /ApEinfo_fwdcolor="#993366"
                     /ApEinfo_revcolor="#993366"
     CDS             2229..2549
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label="F36V mutant of FK506-binding protein FKBP12 CDS"
                     /note="DmrB"
                     /note="binds synthetic ligands that do not bind wild-type
                     FKBP"
                     /note="color: #993366"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
                     /ApEinfo_fwdcolor="#993366"
                     /ApEinfo_revcolor="#993366"
     CDS             2556..2582
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label="HA (human influenza hemagglutinin) epitope tag CDS"
                     /note="HA"
                     /note="color: #993366"
                     /translation="YPYDVPDYA"
                     /ApEinfo_fwdcolor="#993366"
                     /ApEinfo_revcolor="#993366"
     misc_feature    2588..2591
                     /label="Ligation"
                     /label="nonstandard type: ligation"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
     rep_origin      2923..3229
                     /label="F1 ori"
                     /note="color: #9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /ApEinfo_revcolor="#9eafd2"
     primer_bind     complement(4461..4481)
                     /label="M13-rev"
                     /note="color: #85dae9; direction: LEFT"
                     /ApEinfo_fwdcolor="#85dae9; direction: LEFT"
                     /ApEinfo_revcolor="#85dae9; direction: LEFT"
     misc_binding    complement(4487..4509)
                     /label="LacO"
                     /note="color: #84b0dc; direction: LEFT"
                     /ApEinfo_fwdcolor="#84b0dc; direction: LEFT"
                     /ApEinfo_revcolor="#84b0dc; direction: LEFT"
     promoter        complement(4513..4543)
                     /label="lac promoter"
                     /note="color: #c7b0e3; direction: LEFT"
                     /ApEinfo_fwdcolor="#c7b0e3; direction: LEFT"
                     /ApEinfo_revcolor="#c7b0e3; direction: LEFT"
     CDS             complement(5626..6285)
                     /label="AmpR"
                     /note="color: #ffef86; direction: LEFT"
                     /ApEinfo_fwdcolor="#ffef86; direction: LEFT"
                     /ApEinfo_revcolor="#ffef86; direction: LEFT"
     source          1..6619
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
     misc_feature    997..2591
                     /label="RTgb1a N-FKBPF36V-HA (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:j-d0hi8c2"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
     misc_feature    join(3677..7708,1..1000)
                     /label="rt1a pCDNA4-TO (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:o-d81dp1x"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggaacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cgccaccatg ttccagaccg
     1021 ccgccgacaa ggccgccgcc gccaaggaca ccgttgttga caccttgtgc gacgccaagg
     1081 acaccgccaa ggagaaggtg gtgggcgcca aggacgccgg agccgactac gctggcgaga
     1141 agaaggagca gatgagggac tccggaggcg gcggatccgg cggaatggtg tctaaaggcg
     1201 aggaggataa catggctata atcaaggaat ttatgaggtt caaggtgcat atggagggat
     1261 ctgtgaacgg tcacgagttc gaaatcgagg gcgaaggcga ggggcgaccc tatgaaggga
     1321 cacagacggc caaactgaag gtgaccaagg gtggccccct gcctttcgcc tgggacatcc
     1381 tgtcacccca gtttatgtac ggctctaagg cttacgttaa gcaccctgcc gatattccgg
     1441 actacttgaa actgagcttc cctgaagggt tcaagtggga acgggtaatg aattttgagg
     1501 atggcggtgt agtcacagtt acccaggaca gctctcttca ggacggagaa tttatctata
     1561 aggttaaact tcggggcact aacttcccat cagacggccc cgtgatgcag aagaaaacta
     1621 tgggctggga agccagctca gaacgcatgt atcccgagga cggagcactg aagggagaaa
     1681 tcaagcagcg actcaagctg aaggatgggg gacattatga tgctgaggtc aaaaccacct
     1741 ataaggccaa gaaacccgtt cagcttcctg gggcatacaa cgtgaatatc aaactggata
     1801 ttacatccca taacgaagac tataccatcg tggaacagta cgagcgggcc gagggcagac
     1861 atagcacagg gggcatggat gaattgtaca aggcgactag aggcgtccaa gtcgaaacca
     1921 ttagtcccgg cgatggcaga acatttccta aaaggggaca aacatgtgtc gtccattata
     1981 caggcatgtt ggaggacggc aaaaaggtgg acagtagtag agatcgcaat aaacctttca
     2041 aattcatgtt gggaaaacaa gaagtcatta ggggatggga ggagggcgtg gctcaaatgt
     2101 ccgtcggcca acgcgctaag ctcaccatca gccccgacta cgcatacggc gctaccggac
     2161 atcccggaat tattccccct cacgctacct tggtgtttga cgtcgaactg ttgaagctcg
     2221 agactagagg agtgcaggtg gagactatct ccccaggaga cgggcgcacc ttccccaagc
     2281 gcggccagac ctgcgtggtg cactacaccg ggatgcttga agatggaaag aaagttgatt
     2341 cctcccggga cagaaacaag ccctttaagt ttatgctagg caagcaggag gtgatccgag
     2401 gctgggaaga aggggttgcc cagatgagtg tgggtcagag agccaaactg actatatctc
     2461 cagattatgc ctatggtgcc actgggcacc caggcatcat cccaccacat gccactctcg
     2521 tcttcgatgt ggagcttcta aaactggaaa ctagttatcc gtacgacgta ccagactacg
     2581 cataagcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg
     2641 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     2701 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     2761 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     2821 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     2881 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     2941 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     3001 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     3061 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     3121 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     3181 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     3241 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     3301 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     3361 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3421 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     3481 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     3541 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     3601 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     3661 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     3721 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     3781 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     3841 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     3901 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     3961 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
     4021 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     4081 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     4141 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
     4201 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     4261 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     4321 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     4381 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     4441 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     4501 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     4561 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     4621 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     4681 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     4741 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     4801 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     4861 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4921 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4981 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5041 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5101 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5161 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5221 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5281 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     5341 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5401 cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5461 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5521 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     5581 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     5641 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5701 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5761 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5821 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5881 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5941 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     6001 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     6061 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     6121 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     6181 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     6241 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     6301 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     6361 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     6421 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     6481 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     6541 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     6601 aaaagtgcca cctgacgtc
//
