LOCUS       .                       6535 bp    DNA     circular SYN 22-JUN-2021
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 22, 2021 from SnapGene Viewer 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     promoter        311..614
                     /label="CMV enhancer"
                     /note="color: #c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /ApEinfo_revcolor="#c7b0e3"
     misc_feature    820..861
                     /label="teto2x2"
                     /note="color: #f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
     misc_feature    997..1007
                     /label="Geneious type: Editing History Insertion"
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
     misc_feature    997..1000
                     /label="Ligation"
                     /label="nonstandard type: ligation"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
     CDS             1008..1010
                     /label="Start"
                     /note="color: #992b2b"
                     /ApEinfo_fwdcolor="#992b2b"
                     /ApEinfo_revcolor="#992b2b"
                     /translation="M"
     CDS             1011..1076
                     /label="pvLEA22mer_mutant_3"
                     /note="color: #0201fb"
                     /ApEinfo_fwdcolor="#0201fb"
                     /ApEinfo_revcolor="#0201fb"
                     /translation="AKDATVEKAGEAKDATVEKAGE"
     CDS             1077..1100
                     /label="Dan's SGGSGGSGG linker"
                     /note="color: #9e2273"
                     /ApEinfo_fwdcolor="#9e2273"
                     /ApEinfo_revcolor="#9e2273"
                     /translation="SGGGGSGG"
     CDS             1101..1811
                     /label="Dan's mCherry"
                     /note="color: #877fec"
                     /ApEinfo_fwdcolor="#877fec"
                     /ApEinfo_revcolor="#877fec"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKA"
     source          1818..2138
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
     source          2139..2465
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
     source          2466..2501
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
     misc_feature    1812..2507
                     /label="Geneious type: Editing History Insertion"
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
     misc_feature    1812..1817
                     /label="RTgb1a N-FKBPF36V-HA (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:j-d0hi8c2"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
     CDS             1818..2138
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label="F36V mutant of FK506-binding protein FKBP12 CDS"
                     /note="DmrB"
                     /note="binds synthetic ligands that do not bind wild-type
                     FKBP"
                     /note="color: #993366"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
                     /ApEinfo_fwdcolor="#993366"
                     /ApEinfo_revcolor="#993366"
     CDS             2145..2465
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label="F36V mutant of FK506-binding protein FKBP12 CDS"
                     /note="DmrB"
                     /note="binds synthetic ligands that do not bind wild-type
                     FKBP"
                     /note="color: #993366"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
                     /ApEinfo_fwdcolor="#993366"
                     /ApEinfo_revcolor="#993366"
     CDS             2472..2498
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label="HA (human influenza hemagglutinin) epitope tag CDS"
                     /note="HA"
                     /note="color: #993366"
                     /translation="YPYDVPDYA"
                     /ApEinfo_fwdcolor="#993366"
                     /ApEinfo_revcolor="#993366"
     misc_feature    2504..2507
                     /label="Ligation"
                     /label="nonstandard type: ligation"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
     rep_origin      2839..3145
                     /label="F1 ori"
                     /note="color: #9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /ApEinfo_revcolor="#9eafd2"
     primer_bind     complement(4377..4397)
                     /label="M13-rev"
                     /note="color: #85dae9; direction: LEFT"
                     /ApEinfo_fwdcolor="#85dae9; direction: LEFT"
                     /ApEinfo_revcolor="#85dae9; direction: LEFT"
     misc_binding    complement(4403..4425)
                     /label="LacO"
                     /note="color: #84b0dc; direction: LEFT"
                     /ApEinfo_fwdcolor="#84b0dc; direction: LEFT"
                     /ApEinfo_revcolor="#84b0dc; direction: LEFT"
     promoter        complement(4429..4459)
                     /label="lac promoter"
                     /note="color: #c7b0e3; direction: LEFT"
                     /ApEinfo_fwdcolor="#c7b0e3; direction: LEFT"
                     /ApEinfo_revcolor="#c7b0e3; direction: LEFT"
     CDS             complement(5542..6201)
                     /label="AmpR"
                     /note="color: #ffef86; direction: LEFT"
                     /ApEinfo_fwdcolor="#ffef86; direction: LEFT"
                     /ApEinfo_revcolor="#ffef86; direction: LEFT"
     source          1..6535
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
     misc_feature    997..2507
                     /label="RTgb1a N-FKBPF36V-HA (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:j-d0hi8c2"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
     misc_feature    join(3677..7708,1..1000)
                     /label="rt1a pCDNA4-TO (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:o-d81dp1x"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggaacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cgccaccatg gccaaggacg
     1021 caaccgtgga gaaggcagga gaggcaaagg acgccaccgt ggagaaagcc ggggagtccg
     1081 gaggcggcgg atccggcgga atggtgtcta aaggcgagga ggataacatg gctataatca
     1141 aggaatttat gaggttcaag gtgcatatgg agggatctgt gaacggtcac gagttcgaaa
     1201 tcgagggcga aggcgagggg cgaccctatg aagggacaca gacggccaaa ctgaaggtga
     1261 ccaagggtgg ccccctgcct ttcgcctggg acatcctgtc accccagttt atgtacggct
     1321 ctaaggctta cgttaagcac cctgccgata ttccggacta cttgaaactg agcttccctg
     1381 aagggttcaa gtgggaacgg gtaatgaatt ttgaggatgg cggtgtagtc acagttaccc
     1441 aggacagctc tcttcaggac ggagaattta tctataaggt taaacttcgg ggcactaact
     1501 tcccatcaga cggccccgtg atgcagaaga aaactatggg ctgggaagcc agctcagaac
     1561 gcatgtatcc cgaggacgga gcactgaagg gagaaatcaa gcagcgactc aagctgaagg
     1621 atgggggaca ttatgatgct gaggtcaaaa ccacctataa ggccaagaaa cccgttcagc
     1681 ttcctggggc atacaacgtg aatatcaaac tggatattac atcccataac gaagactata
     1741 ccatcgtgga acagtacgag cgggccgagg gcagacatag cacagggggc atggatgaat
     1801 tgtacaaggc gactagaggc gtccaagtcg aaaccattag tcccggcgat ggcagaacat
     1861 ttcctaaaag gggacaaaca tgtgtcgtcc attatacagg catgttggag gacggcaaaa
     1921 aggtggacag tagtagagat cgcaataaac ctttcaaatt catgttggga aaacaagaag
     1981 tcattagggg atgggaggag ggcgtggctc aaatgtccgt cggccaacgc gctaagctca
     2041 ccatcagccc cgactacgca tacggcgcta ccggacatcc cggaattatt ccccctcacg
     2101 ctaccttggt gtttgacgtc gaactgttga agctcgagac tagaggagtg caggtggaga
     2161 ctatctcccc aggagacggg cgcaccttcc ccaagcgcgg ccagacctgc gtggtgcact
     2221 acaccgggat gcttgaagat ggaaagaaag ttgattcctc ccgggacaga aacaagccct
     2281 ttaagtttat gctaggcaag caggaggtga tccgaggctg ggaagaaggg gttgcccaga
     2341 tgagtgtggg tcagagagcc aaactgacta tatctccaga ttatgcctat ggtgccactg
     2401 ggcacccagg catcatccca ccacatgcca ctctcgtctt cgatgtggag cttctaaaac
     2461 tggaaactag ttatccgtac gacgtaccag actacgcata agcggccgct cgagtctaga
     2521 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     2581 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     2641 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     2701 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     2761 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     2821 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     2881 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     2941 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     3001 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     3061 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     3121 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     3181 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     3241 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     3301 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     3361 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3421 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     3481 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     3541 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     3601 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     3661 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac
     3721 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
     3781 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
     3841 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
     3901 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
     3961 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
     4021 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
     4081 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     4141 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     4201 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     4261 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     4321 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     4381 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     4441 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     4501 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     4561 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     4621 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4681 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4741 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4801 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4861 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4921 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4981 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5041 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5101 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5161 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5221 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5281 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca
     5341 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     5401 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5461 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5521 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5581 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5641 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5701 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5761 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5821 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     5881 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5941 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     6001 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     6061 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     6121 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     6181 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6241 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6301 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6361 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6421 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     6481 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
