LOCUS       .                       6736 bp    DNA     circular SYN 22-JUN-2021
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 22, 2021 from SnapGene Viewer 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     promoter        311..614
                     /label="CMV enhancer"
                     /note="color: #c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /ApEinfo_revcolor="#c7b0e3"
     misc_feature    820..861
                     /label="teto2x2"
                     /note="color: #f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
     misc_feature    997..1007
                     /label="Geneious type: Editing History Insertion"
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
     misc_feature    997..1000
                     /label="Ligation"
                     /label="nonstandard type: ligation"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
     CDS             1008..1010
                     /label="Start"
                     /note="color: #992b2b"
                     /ApEinfo_fwdcolor="#992b2b"
                     /ApEinfo_revcolor="#992b2b"
                     /translation="M"
     CDS             1011..1277
                     /label="CAHS1_PARRC_101-189"
                     /note="color: #40ca29"
                     /ApEinfo_fwdcolor="#40ca29"
                     /ApEinfo_revcolor="#40ca29"
                     /translation="AKTREQEMLSANYQKEVERKTEAYRKQQEVEADKIRKELEKQHLR
                     DVEFRKDIVEMAIENQKKMIDVESRYAKKDMDRERVKVRMMLEQ"
     CDS             1278..1301
                     /label="Dan's SGGSGGSGG linker"
                     /note="color: #9e2273"
                     /ApEinfo_fwdcolor="#9e2273"
                     /ApEinfo_revcolor="#9e2273"
                     /translation="SGGGGSGG"
     CDS             1302..2012
                     /label="Dan's mCherry"
                     /note="color: #877fec"
                     /ApEinfo_fwdcolor="#877fec"
                     /ApEinfo_revcolor="#877fec"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKA"
     source          2019..2339
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
     source          2340..2666
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
     source          2667..2702
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
     misc_feature    2013..2708
                     /label="Geneious type: Editing History Insertion"
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
     misc_feature    2013..2018
                     /label="RTgb1a N-FKBPF36V-HA (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:j-d0hi8c2"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
     CDS             2019..2339
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label="F36V mutant of FK506-binding protein FKBP12 CDS"
                     /note="DmrB"
                     /note="binds synthetic ligands that do not bind wild-type
                     FKBP"
                     /note="color: #993366"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
                     /ApEinfo_fwdcolor="#993366"
                     /ApEinfo_revcolor="#993366"
     CDS             2346..2666
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label="F36V mutant of FK506-binding protein FKBP12 CDS"
                     /note="DmrB"
                     /note="binds synthetic ligands that do not bind wild-type
                     FKBP"
                     /note="color: #993366"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
                     /ApEinfo_fwdcolor="#993366"
                     /ApEinfo_revcolor="#993366"
     CDS             2673..2699
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label="HA (human influenza hemagglutinin) epitope tag CDS"
                     /note="HA"
                     /note="color: #993366"
                     /translation="YPYDVPDYA"
                     /ApEinfo_fwdcolor="#993366"
                     /ApEinfo_revcolor="#993366"
     misc_feature    2705..2708
                     /label="Ligation"
                     /label="nonstandard type: ligation"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
     rep_origin      3040..3346
                     /label="F1 ori"
                     /note="color: #9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /ApEinfo_revcolor="#9eafd2"
     primer_bind     complement(4578..4598)
                     /label="M13-rev"
                     /note="color: #85dae9; direction: LEFT"
                     /ApEinfo_fwdcolor="#85dae9; direction: LEFT"
                     /ApEinfo_revcolor="#85dae9; direction: LEFT"
     misc_binding    complement(4604..4626)
                     /label="LacO"
                     /note="color: #84b0dc; direction: LEFT"
                     /ApEinfo_fwdcolor="#84b0dc; direction: LEFT"
                     /ApEinfo_revcolor="#84b0dc; direction: LEFT"
     promoter        complement(4630..4660)
                     /label="lac promoter"
                     /note="color: #c7b0e3; direction: LEFT"
                     /ApEinfo_fwdcolor="#c7b0e3; direction: LEFT"
                     /ApEinfo_revcolor="#c7b0e3; direction: LEFT"
     CDS             complement(5743..6402)
                     /label="AmpR"
                     /note="color: #ffef86; direction: LEFT"
                     /ApEinfo_fwdcolor="#ffef86; direction: LEFT"
                     /ApEinfo_revcolor="#ffef86; direction: LEFT"
     source          1..6736
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
     misc_feature    997..2708
                     /label="RTgb1a N-FKBPF36V-HA (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:j-d0hi8c2"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
     misc_feature    join(3677..7708,1..1000)
                     /label="rt1a pCDNA4-TO (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:o-d81dp1x"
                     /note="color: #a6acb3"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggaacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cgccaccatg gccaaaacgc
     1021 gcgaacagga gatgctgtct gctaactatc aaaaagaagt tgagcgcaaa actgaagcct
     1081 atcggaagca gcaagaagtg gaggcagaca aaattcggaa agagctggaa aagcagcatc
     1141 tgcgtgacgt ggagttccgc aaagatattg tagagatggc aatcgaaaac cagaaaaaaa
     1201 tgattgatgt cgaaagccgt tatgcaaaaa aagatatgga ccgcgaacgt gtgaaagtcc
     1261 gtatgatgct cgagcagtcc ggaggcggcg gatccggcgg aatggtgtct aaaggcgagg
     1321 aggataacat ggctataatc aaggaattta tgaggttcaa ggtgcatatg gagggatctg
     1381 tgaacggtca cgagttcgaa atcgagggcg aaggcgaggg gcgaccctat gaagggacac
     1441 agacggccaa actgaaggtg accaagggtg gccccctgcc tttcgcctgg gacatcctgt
     1501 caccccagtt tatgtacggc tctaaggctt acgttaagca ccctgccgat attccggact
     1561 acttgaaact gagcttccct gaagggttca agtgggaacg ggtaatgaat tttgaggatg
     1621 gcggtgtagt cacagttacc caggacagct ctcttcagga cggagaattt atctataagg
     1681 ttaaacttcg gggcactaac ttcccatcag acggccccgt gatgcagaag aaaactatgg
     1741 gctgggaagc cagctcagaa cgcatgtatc ccgaggacgg agcactgaag ggagaaatca
     1801 agcagcgact caagctgaag gatgggggac attatgatgc tgaggtcaaa accacctata
     1861 aggccaagaa acccgttcag cttcctgggg catacaacgt gaatatcaaa ctggatatta
     1921 catcccataa cgaagactat accatcgtgg aacagtacga gcgggccgag ggcagacata
     1981 gcacaggggg catggatgaa ttgtacaagg cgactagagg cgtccaagtc gaaaccatta
     2041 gtcccggcga tggcagaaca tttcctaaaa ggggacaaac atgtgtcgtc cattatacag
     2101 gcatgttgga ggacggcaaa aaggtggaca gtagtagaga tcgcaataaa cctttcaaat
     2161 tcatgttggg aaaacaagaa gtcattaggg gatgggagga gggcgtggct caaatgtccg
     2221 tcggccaacg cgctaagctc accatcagcc ccgactacgc atacggcgct accggacatc
     2281 ccggaattat tccccctcac gctaccttgg tgtttgacgt cgaactgttg aagctcgaga
     2341 ctagaggagt gcaggtggag actatctccc caggagacgg gcgcaccttc cccaagcgcg
     2401 gccagacctg cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa gttgattcct
     2461 cccgggacag aaacaagccc tttaagttta tgctaggcaa gcaggaggtg atccgaggct
     2521 gggaagaagg ggttgcccag atgagtgtgg gtcagagagc caaactgact atatctccag
     2581 attatgccta tggtgccact gggcacccag gcatcatccc accacatgcc actctcgtct
     2641 tcgatgtgga gcttctaaaa ctggaaacta gttatccgta cgacgtacca gactacgcat
     2701 aagcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct
     2761 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     2821 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     2881 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     2941 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     3001 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     3061 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     3121 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     3181 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     3241 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     3301 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     3361 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     3421 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     3481 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3541 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3601 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     3661 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3721 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     3781 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     3841 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     3901 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     3961 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     4021 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     4081 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     4141 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     4201 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     4261 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     4321 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     4381 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     4441 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     4501 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     4561 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     4621 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     4681 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     4741 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4801 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4861 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4921 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4981 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     5041 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     5101 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     5161 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5221 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     5281 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     5341 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5401 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     5461 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5521 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5581 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5641 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5701 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5761 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5821 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5881 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5941 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     6001 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     6061 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     6121 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     6181 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     6241 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     6301 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     6361 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     6421 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     6481 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     6541 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6601 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6661 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6721 agtgccacct gacgtc
//
