LOCUS       .                       6959 bp    ds-DNA  circular SYN 30-OCT-2019
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 30, 2019 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     enhancer        234..613
                     /label="CMV enhancer"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     promoter        614..817
                     /label="CMV promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     Msc             862..879
                     /label="T7 promoter"
                     /note="color: #FF0000"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
     CDS             904..906
                     /label="Start"
                     /note="color: #1c598e"
                     /ApEinfo_fwdcolor="#1c598e"
                     /ApEinfo_revcolor="#1c598e"
                     /translation="M"
     CDS             907..1854
                     /label="ApoE3"
                     /note="color: #882284"
                     /ApEinfo_fwdcolor="#882284"
                     /ApEinfo_revcolor="#882284"
                     /translation="KVLWAALLVTFLAGCQAKVEQAVETEPEPELRQQTEWQSGQRWEL
                     ALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAE
                     ETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRK
                     RLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQE
                     RAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSW
                     FEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH"
     CDS             1855..1878
                     /label="Dan's SGGSGGSGG linker"
                     /note="color: #ba30ca"
                     /ApEinfo_fwdcolor="#ba30ca"
                     /ApEinfo_revcolor="#ba30ca"
                     /translation="SGGGGSGG"
     CDS             1879..2589
                     /label="Dan's mCherry"
                     /note="color: #f900a8"
                     /ApEinfo_fwdcolor="#f900a8"
                     /ApEinfo_revcolor="#f900a8"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKA"
     CDS             2590..2622
                     /label="Dan's mCherry to SspB linker"
                     /note="color: #943b6e"
                     /ApEinfo_fwdcolor="#943b6e"
                     /ApEinfo_revcolor="#943b6e"
                     /translation="AGSGGTSGGSS"
     CDS             2623..2949
                     /label="Dan's SspB"
                     /note="color: #311734"
                     /ApEinfo_fwdcolor="#311734"
                     /ApEinfo_revcolor="#311734"
                     /translation="PKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQIV
                     LNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEIYD
                     ELNIG"
     CDS             2950..2952
                     /label="stop ochre TAA"
                     /note="color: #00de14"
                     /ApEinfo_fwdcolor="#00de14"
                     /ApEinfo_revcolor="#00de14"
                     /translation="*"
     polyA_signal    2972..3196
                     /label="bGH poly(A) signal"
                     /note="color: #b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
     rep_origin      3242..3670
                     /label="f1 ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     promoter        3684..4013
                     /label="SV40 promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     rep_origin      3864..3999
                     /label="SV40 ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     promoter        4061..4108
                     /label="EM7 promoter"
                     /note="color: #c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
     CDS             4127..4501
                     /label="BleoR"
                     /note="color: #b7e6d7; direction: RIGHT"
                     /ApEinfo_fwdcolor="#b7e6d7; direction: RIGHT"
                     /ApEinfo_revcolor="#b7e6d7; direction: RIGHT"
     polyA_signal    4631..4752
                     /label="SV40 poly(A) signal"
                     /note="color: #b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
     primer_bind     complement(4801..4817)
                     /label="M13 rev"
                     /note="color: #a020f0; direction: LEFT"
                     /ApEinfo_fwdcolor="#a020f0; direction: LEFT"
                     /ApEinfo_revcolor="#a020f0; direction: LEFT"
     protein_bind    4825..4841
                     /label="lac operator"
                     /note="color: #31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /ApEinfo_revcolor="#31849b"
     promoter        complement(4849..4879)
                     /label="lac promoter"
                     /note="color: #c6c9d1; direction: LEFT"
                     /ApEinfo_fwdcolor="#c6c9d1; direction: LEFT"
                     /ApEinfo_revcolor="#c6c9d1; direction: LEFT"
     protein_bind    4894..4915
                     /label="CAP binding site"
                     /note="color: #31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /ApEinfo_revcolor="#31849b"
     rep_origin      complement(5203..5791)
                     /direction=LEFT
                     /label="ori"
                     /note="color: #ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     CDS             complement(5962..6822)
                     /label="AmpR"
                     /note="color: #b7e6d7; direction: LEFT"
                     /ApEinfo_fwdcolor="#b7e6d7; direction: LEFT"
                     /ApEinfo_revcolor="#b7e6d7; direction: LEFT"
     promoter        complement(6823..6927)
                     /label="AmpR promoter"
                     /note="color: #c6c9d1; direction: LEFT"
                     /ApEinfo_fwdcolor="#c6c9d1; direction: LEFT"
                     /ApEinfo_revcolor="#c6c9d1; direction: LEFT"
     source          1..6959
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
     primer          1855..1872
                     /label="MTV0077"
                     /note="color: #FF0000"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
     primer          complement(885..903)
                     /label="MTV0078"
                     /note="color: #FF0000"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
     primer          complement(886..903)
                     /label="MTV0100"
                     /note="color: #FF0000"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
     primer          1855..1872
                     /label="MTV0123"
                     /note="color: #FF0000"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
     primer          complement(885..906)
                     /label="MTV0209"
                     /note="color: #FF0000"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
     primer          complement(887..906)
                     /label="MTV0211"
                     /note="color: #FF0000"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
     primer          884..927
                     /label="MTV0214"
                     /note="color: #FF0000"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
     primer          complement(1837..1874)
                     /label="MTV0215"
                     /note="color: #FF0000"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
     primer          884..927
                     /label="MTV0216"
                     /note="color: #FF0000"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
     primer          complement(1837..1874)
                     /label="MTV0219"
                     /note="color: #FF0000"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
     primer          complement(2571..2589)
                     /label="MTV0223"
                     /note="color: #FF0000"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
      841 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcc
      901 accatgaaag tgttatgggc agcattgttg gtgacatttt tagctgggtg ccaggccaaa
      961 gtggaacaag cagtggaaac agaacctgaa cctgaactga gacagcagac cgaatggcaa
     1021 agtggccaga gatgggaact ggccctgggg agattttggg actatctgag atgggtgcag
     1081 accctgagcg agcaggtgca ggaggagctc ctgagctctc aggtgacaca ggaactgaga
     1141 gccctgatgg acgagactat gaaggaactg aaggcctaca aatctgagct ggaagagcag
     1201 ctgacccccg tggctgaaga gacccgcgct cgcttgtcca aagagctcca ggccgcccag
     1261 gcccggctgg gtgccgatat ggaggacgtc tgcggccgcc tggtccagta ccgcggggaa
     1321 gtgcaggcta tgctggggca gtctacagag gagctccggg tcagactggc ctctcacctg
     1381 cggaagctga ggaagaggtt gctccgcgac gccgacgacc tccagaagcg cctggctgtg
     1441 taccaggccg gcgcccggga aggcgccgag cgcggactct ccgctatcag ggagcggctc
     1501 ggccctctgg ttgaacaggg cagagtcagg gctgccactg tggggagcct cgccggacag
     1561 cccctccagg agagagccca ggcctggggc gagcgcctgc gagctagaat ggaggagatg
     1621 gggtccagaa cccgcgaccg cctggacgaa gtgaaggagc aggtggccga ggtgcgtgcc
     1681 aaactggagg aacaggccca gcagatccgc ctccaggctg aggccttcca ggccagactg
     1741 aagagctggt ttgagcctct tgtggaggat atgcagcggc agtgggccgg gctggtcgag
     1801 aaggtgcagg ccgccgtggg cacttccgcc gcccctgtcc ccagcgacaa ccattccgga
     1861 ggcggcggat ccggcggaat ggtgtctaaa ggcgaggagg ataacatggc tataatcaag
     1921 gaatttatga ggttcaaggt gcatatggag ggatctgtga acggtcacga gttcgaaatc
     1981 gagggcgaag gcgaggggcg accctatgaa gggacacaga cggccaaact gaaggtgacc
     2041 aagggtggcc ccctgccttt cgcctgggac atcctgtcac cccagtttat gtacggctct
     2101 aaggcttacg ttaagcaccc tgccgatatt ccggactact tgaaactgag cttccctgaa
     2161 gggttcaagt gggaacgggt aatgaatttt gaggatggcg gtgtagtcac agttacccag
     2221 gacagctctc ttcaggacgg agaatttatc tataaggtta aacttcgggg cactaacttc
     2281 ccatcagacg gccccgtgat gcagaagaaa actatgggct gggaagccag ctcagaacgc
     2341 atgtatcccg aggacggagc actgaaggga gaaatcaagc agcgactcaa gctgaaggat
     2401 gggggacatt atgatgctga ggtcaaaacc acctataagg ccaagaaacc cgttcagctt
     2461 cctggggcat acaacgtgaa tatcaaactg gatattacat cccataacga agactatacc
     2521 atcgtggaac agtacgagcg ggccgagggc agacatagca cagggggcat ggatgaattg
     2581 tacaaggcgg ccggatccgg tggtacctct ggaggtagct ccccgaaacg ccctaagctg
     2641 ctgcgtgaat attacgattg gctggttgat aacagcttta ccccatatct ggtggtggat
     2701 gccacatacc tgggcgtgaa cgtgcccgtg gagtatgtga aagacggtca gatcgtgctg
     2761 aatctgtctg caagtgcgac cggcaacctg caactgacaa atgattttat ccagttcaac
     2821 gcccgcttta agggcgtgtc tcgtgaactg tatatcccga tgggtgccgc tctggccatt
     2881 tacgctcgcg agaacggcga tggtgtgatg ttcgaaccag aagaaatcta tgacgagctg
     2941 aatattggtt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     3001 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     3061 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     3121 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     3181 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     3241 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     3301 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     3361 acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt
     3421 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     3481 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     3541 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     3601 taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     3661 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     3721 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     3781 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3841 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     3901 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     3961 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     4021 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     4081 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac
     4141 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
     4201 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
     4261 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
     4321 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
     4381 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
     4441 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
     4501 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     4561 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     4621 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     4681 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     4741 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     4801 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     4861 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     4921 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     4981 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5041 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5101 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5161 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5221 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5281 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5341 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
     5401 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5461 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5521 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5581 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5641 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5701 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     5761 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     5821 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     5881 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     5941 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6001 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6061 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6121 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6181 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     6241 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     6301 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     6361 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     6421 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     6481 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     6541 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     6601 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     6661 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     6721 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     6781 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     6841 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     6901 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcg
//
