LOCUS       Exported                8529 bp DNA     circular SYN 13-APR-2022
DEFINITION  pDisplay-BirA-ER.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 13, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8529
                     /mol_type="other DNA"
                     /organism="pDisplay-BirA-ER"
     promoter        11..627
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter
     misc_feature    90..377
                     /label=CAG_enhancer
                     /label=CAG_enhancer
                     /label=CAG_enhancer
                     /label=CAG_enhancer
                     /label=CAG_enhancer
                     /label=CAG_enhancer
                     /label=CAG_enhancer
     misc_feature    544..564
                     /label=CMV_fwd_primer
                     /label=CMV_fwd_primer
                     /label=CMV_fwd_primer
                     /label=CMV_fwd_primer
                     /label=CMV_fwd_primer
                     /label=CMV_fwd_primer
                     /label=CMV_fwd_primer
     primer_bind     549..566
                     /label=PCMV5F
     promoter        638..656
                     /label=T7_promoter
                     /label=T7_promoter
                     /label=T7_promoter
                     /label=T7_promoter
                     /label=T7_promoter
                     /label=T7_promoter
                     /label=T7_promoter
     primer_bind     687..739
                     /label=F-EcoRI-IgK
     CDS             737..4072
                     /label=ORF
     misc_feature    737..799
                     /label=IgK_secretion
                     /label=IgK_secretion
                     /label=IgK_secretion
                     /label=IgK_secretion
                     /label=IgK_secretion
                     /label=IgK_secretion
                     /label=IgK_secretion
     misc_feature    737..799
                     /label=Ig K leader sequence
                     /label=Ig K leader sequence
     primer_bind     803..832
                     /label=F-pdisplay-AgeI-OXTR
     primer_bind     complement(804..826)
                     /label=R-pdisplayJM
     gene            833..2719
                     /label=Bovine-OXTR
     misc_feature    1517..1519
                     /label=R14
     misc_feature    1520..1522
                     /label=R13
     misc_feature    1523..1525
                     /label=R12
     misc_feature    1526..1528
                     /label=R11
     misc_feature    1529..1531
                     /label=R1
     misc_feature    1532..1534
                     /label=R23
     misc_feature    1535..1537
                     /label=R22-5.74
     misc_feature    1538..2338
                     /label=NE2h-ICL3
     misc_feature    1538..1540
                     /label=ICL3-G284
     gene            1574..2317
                     /label=GG-cpEGFP-TGAAA
     primer_bind     complement(1831..1850)
                     /label=R-CAAX-gb-mcherry
     primer_bind     1867..1888
                     /label=F-IRES-NcoI-gb-mcherry
     misc_feature    2339..2341
                     /label=F21-6.24
     misc_feature    2342..2344
                     /label=F22
     misc_feature    2348..2350
                     /label=F2
     misc_feature    2360..2362
                     /label=F3
     misc_feature    2372..2374
                     /label=F4
     primer_bind     2468..2485
                     /label=F_bOXTR_Cter_SeqF
     primer_bind     complement(2720..2750)
                     /label=R-IRES-Nhel-gb-OXTR
     primer_bind     2726..2741
                     /label=F-IRES
     primer_bind     3286..3334
                     /label=F-IRES-NcoI-gb-mcherry
     primer_bind     complement(3290..3310)
                     /label=R-IRES
     gene            3317..4021
                     /label=mcherry
     primer_bind     complement(4003..4061)
                     /label=R-CAAX-gb-mcherry
     primer_bind     4037..4093
                     /label=F-CAAX-gb-pdisplayJM
     primer_bind     complement(4045..4094)
                     /label=R-pdisplay-gb-CAAX
     primer_bind     complement(4070..4094)
                     /label=overlap-pdisplayJM TAA
     primer_bind     4070..4093
                     /label=F-pdisplayJM
     misc_feature    4109..4118
                     /label=PDGFR transmembrane domain
                     /label=PDGFR transmembrane domain
     misc_feature    complement(4279..4296)
                     /label=BGH_rev_primer
                     /label=BGH_rev_primer
                     /label=BGH_rev_primer
                     /label=BGH_rev_primer
                     /label=BGH_rev_primer
                     /label=BGH_rev_primer
                     /label=BGH_rev_primer
                     /note="/translation=METDTLLLWVLLLWVPGSTGDYPYDVPDYAGAQPARSMK
                     DNTVP 
                     LKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQ 
                     LLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKW 
                     FSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQD 
                     RKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLA 
                     AMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGA 
                     LLLEQDGIIKPWMGGEISLRSAEKGSTSGSGGSKDEL*"
     terminator      4282..4509
                     /label=bGH_PA_terminator
                     /label=bGH_PA_terminator
                     /label=bGH_PA_terminator
                     /label=bGH_PA_terminator
                     /label=bGH_PA_terminator
                     /label=bGH_PA_terminator
                     /label=bGH_PA_terminator
                     /note="/translation=METDTLLLWVLLLWVPGSTGDYPYDVPDYAGAQPARSMK
                     DNTVP 
                     LKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQ 
                     LLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKW 
                     FSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQD 
                     RKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLA 
                     AMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGA 
                     LLLEQDGIIKPWMGGEISLRSAEKGSTSGSGGSKDEL*"
     rep_origin      complement(4626..5245)
                     /direction=LEFT
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /note="/translation=METDTLLLWVLLLWVPGSTGDYPYDVPDYAGAQPARSMK
                     DNTVP 
                     LKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQ 
                     LLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKW 
                     FSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQD 
                     RKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLA 
                     AMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGA 
                     LLLEQDGIIKPWMGGEISLRSAEKGSTSGSGGSKDEL*"
     primer_bind     4700..4717
                     /label=f-xa.7
     primer_bind     complement(4702..4719)
                     /label=f-xa.J
     primer_bind     complement(5168..5185)
                     /label=f-xa.G
     terminator      complement(5393..5662)
                     /label=TK_PA_terminator
                     /label=TK_PA_terminator
                     /label=TK_PA_terminator
                     /label=TK_PA_terminator
                     /label=TK_PA_terminator
                     /label=TK_PA_terminator
                     /label=TK_PA_terminator
                     /note="/translation=METDTLLLWVLLLWVPGSTGDYPYDVPDYAGAQPARSMK
                     DNTVP 
                     LKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQ 
                     LLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKW 
                     FSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQD 
                     RKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLA 
                     AMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGA 
                     LLLEQDGIIKPWMGGEISLRSAEKGSTSGSGGSKDEL*"
     CDS             5786..6316
                     /codon_start=1
                     /label=ORF frame 2
                     /label=ORF frame 2
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEAV
                     SPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILAVG
                     HARLEPGEQFGWREPLMLLIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQPPHCI
                     SHDGYFLGRSKVR"
     CDS             complement(5837..6625)
                     /codon_start=1
                     /label=ORF frame 3
                     /label=ORF frame 3
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEHQGL
                     APAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALA
                     TRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    complement(6731..6750)
                     /label=SV40pro_F_primer
                     /label=SV40pro_F_primer
                     /label=SV40pro_F_primer
                     /label=SV40pro_F_primer
                     /label=SV40pro_F_primer
                     /label=SV40pro_F_primer
                     /label=SV40pro_F_primer
                     /note="/translation=MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRL
                     SAQGR 
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL 
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEH 
                     QGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD 
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     rep_origin      complement(6735..6812)
                     /direction=LEFT
                     /label=SV40_origin
                     /label=SV40_origin
                     /label=SV40_origin
                     /label=SV40_origin
                     /label=SV40_origin
                     /label=SV40_origin
                     /label=SV40_origin
                     /note="/translation=MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRL
                     SAQGR 
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL 
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEH 
                     QGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD 
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     misc_feature    6977..6997
                     /label=pBABE_3_primer
                     /label=pBABE_3_primer
                     /label=pBABE_3_primer
                     /label=pBABE_3_primer
                     /label=pBABE_3_primer
                     /label=pBABE_3_primer
                     /label=pBABE_3_primer
                     /note="/translation=MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRL
                     SAQGR 
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL 
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEH 
                     QGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD 
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            complement(7080..7940)
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /note="/translation=MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRL
                     SAQGR 
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL 
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEH 
                     QGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD 
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(7080..7940)
                     /codon_start=1
                     /label=ORF frame 2
                     /label=ORF frame 2
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7291..7308
                     /label=f-xa.11
     primer_bind     complement(7297..7314)
                     /label=fxaE
     primer_bind     complement(7740..7757)
                     /label=f-xa.C
     primer_bind     7916..7933
                     /label=pRLTK.R
     promoter        complement(7982..8010)
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     rep_origin      8089..8395
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     primer_bind     8171..8188
                     /label=f-xa.15
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg
      721 cttggggata tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt
      781 tccaggttcc actggtgaca caagtttgta caaaaaagtt ggcaccaccg gtgagggtgc
      841 gtttgcggct aactggagcg ctgaggcggt caacgggagc gcggcgccgc cgggaaccga
      901 gggcaatcgc actgccgggc cgccacagcg caacgaggcc ctggcgcggg tggaggtggc
      961 cgtgctgtgc ctcatcctgt tcctggcgct gagcggcaac gcgtgcgtgc tgctagcgct
     1021 gcgcaccacg cgccacaagc actcgcgcct cttcttcttc atgaagcacc tgagcatagc
     1081 cgacctggta gtggcggtgt tccaggtgct gccgcagctt ctgtgggaca tcacgttccg
     1141 cttctacggg cccgacctgc tgtgccgcct cgtcaagtac ctgcaggttg tgggcatgtt
     1201 cgcgtccacc tacctgctgc tgctcatgtc gctcgaccgc tgcctggcca tctgccagcc
     1261 gctgcgctcg ctgagccgcc gcaccgaccg cctggcggta ctcgtcacat ggctcggctg
     1321 cctggtggcc agcgcgccgc aggtgcacat cttctcgctg cgcgaggtgg ccgacggtgt
     1381 cttcgactgc tgggccgttt tcattcaacc ctgggggccc aaggcctaca tcacgtggat
     1441 cacgctcgcc gtctacattg tgcccgtcat cgtgcttgcc acctgctatg gccttatcag
     1501 cttcaagatc tggcagaatt tacggctcaa gacggcgggg ccgcgcgaca ccgacgcgct
     1561 ggacctggag gagggaggaa acgtctatat caaggccgac aagcagaaga acggcatcaa
     1621 ggcgaacttc cacatccgcc acaacatcga ggacggcggc gtgcagctcg cctaccacta
     1681 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     1741 cgtgcagtcc aaactttcga aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     1801 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg gcggtaccgg
     1861 agggagcatg gtgagaaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     1921 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggtg agggcgatgc
     1981 caccgagggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2041 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2101 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacatcc aggagcgcac
     2161 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2221 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     2281 ggggcacaag ctggagtaca acaccggagc agcagcacgc tggcgcgggc ggcagaacaa
     2341 gctcatctct aaggccaaga tccgcacggt caagatgacc ttcatcgtcg tgctggcctt
     2401 catcgtgtgc tggacgccat tctttttcgt gcagatgtgg agtgtctggg atgccgatgc
     2461 gcccaaggaa gcctcacctt tcatcatcgc catgctcctg gccagcctca acagctgctg
     2521 caacccctgg atctacatgc tcttcacggg ccacctcttc caagaacttg tgcagcgctt
     2581 cctctgctgc tcattccgcc gcctgaaagg cagccggcct ggggagacaa gcgtcagcaa
     2641 aaagagcaac tcgtctacct ttgtcctgag ccagtacagc tccagccaga gaagatgctc
     2701 gcagccatcc acgctgtgag ctagcgcccc tctccctccc ccccccctaa cgttactggc
     2761 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     2821 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     2881 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     2941 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     3001 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     3061 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     3121 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     3181 atgggatctg atctggggcc tcggtacaca tgctttacat gtgtttagtc gaggttaaaa
     3241 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     3301 tatggccaca accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     3361 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     3421 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     3481 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     3541 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     3601 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     3661 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     3721 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     3781 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     3841 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     3901 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     3961 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     4021 ggatggtaaa aagaagaaaa agaagtcaaa gacaaagtgt gtaattatgt aagtcgacga
     4081 acaaaaactc atctcagaag aggatctgaa tgctgtgggc caggacacgc aggaggtcat
     4141 cgtggtgcca cactccttgc cctttaaggt ggtggtgatc tcagccatcc tggccctggt
     4201 ggtgctcacc atcatctccc ttatcatcct catcatgctt ggcagaagaa gccacgttag
     4261 gcggccgctc gagatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     4321 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     4381 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     4441 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     4501 gctctatggc ttctgaggcg gaaagaacca gtggcggtaa tacggttatc cacagaatca
     4561 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     4621 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     4681 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     4741 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     4801 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     4861 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     4921 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     4981 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     5041 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     5101 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     5161 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     5221 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     5281 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     5341 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaacctga ggctatggca
     5401 gggcctgccg ccccgacgtt ggctgcgagc cctgggcctt cacccgaact tggggggtgg
     5461 ggtggggaaa aggaagaaac gcgggcgtat tggccccaat ggggtctcgg tggggtatcg
     5521 acagagtgcc agccctggga ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc
     5581 gttttattct gtctttttat tgccgtcata gcgcgggttc cttccggtat tgtctccttc
     5641 cgtgtttcag ttagcctccc cctagggtgg gcgaagaact ccagcatgag atccccgcgc
     5701 tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag
     5761 gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg
     5821 aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
     5881 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
     5941 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
     6001 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
     6061 catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg agcctggcga
     6121 acagttcggc tggcgcgagc ccctgatgct cttgatcatc ctgatcgaca agaccggctt
     6181 ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag
     6241 ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag
     6301 gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc
     6361 ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc
     6421 acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg tcggtcttga
     6481 caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga
     6541 ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg
     6601 cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcgatct
     6661 ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc
     6721 gaggaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa
     6781 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt
     6841 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     6901 ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     6961 cctggggact ttccacaccc taactgacac acattccaca gctggttctt tccgcctcag
     7021 gactcttcct ttttcaataa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7081 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7141 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     7201 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     7261 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     7321 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     7381 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7441 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     7501 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     7561 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     7621 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     7681 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     7741 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     7801 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     7861 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     7921 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     7981 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     8041 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     8101 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     8161 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     8221 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     8281 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     8341 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     8401 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     8461 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     8521 acaatttac
//
