LOCUS       Exported                8472 bp DNA     circular SYN 13-APR-2022
DEFINITION  pDisplay-BirA-ER.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 13, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8472
                     /mol_type="other DNA"
                     /organism="pDisplay-BirA-ER"
     promoter        11..627
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter
     misc_feature    90..377
                     /label=CAG_enhancer
                     /label=CAG_enhancer
                     /label=CAG_enhancer
                     /label=CAG_enhancer
                     /label=CAG_enhancer
                     /label=CAG_enhancer
                     /label=CAG_enhancer
     misc_feature    544..564
                     /label=CMV_fwd_primer
                     /label=CMV_fwd_primer
                     /label=CMV_fwd_primer
                     /label=CMV_fwd_primer
                     /label=CMV_fwd_primer
                     /label=CMV_fwd_primer
                     /label=CMV_fwd_primer
     primer_bind     549..566
                     /label=PCMV5F
     promoter        638..656
                     /label=T7_promoter
                     /label=T7_promoter
                     /label=T7_promoter
                     /label=T7_promoter
                     /label=T7_promoter
                     /label=T7_promoter
                     /label=T7_promoter
     CDS             737..4015
                     /label=ORF
     misc_feature    737..799
                     /label=IgK_secretion
                     /label=IgK_secretion
                     /label=IgK_secretion
                     /label=IgK_secretion
                     /label=IgK_secretion
                     /label=IgK_secretion
                     /label=IgK_secretion
     misc_feature    737..799
                     /label=Ig K leader sequence
                     /label=Ig K leader sequence
     primer_bind     803..832
                     /label=F-pdisplay-AgeI-OXTR
     primer_bind     complement(804..826)
                     /label=R-pdisplayJM
     gene            833..2662
                     /label=OXTR
     misc_feature    1517..1519
                     /label=R14
     misc_feature    1520..1522
                     /label=R13
     misc_feature    1523..1525
                     /label=R12
     misc_feature    1526..1528
                     /label=R11
     misc_feature    1529..1531
                     /label=R1
     misc_feature    1532..1534
                     /label=R23
     misc_feature    1535..1537
                     /label=R22-5.74
     gene            1538..2281
                     /label=GG-cpEGFP-TGAAA
     primer_bind     complement(1795..1814)
                     /label=R-CAAX-gb-mcherry
     primer_bind     1831..1852
                     /label=F-IRES-NcoI-gb-mcherry
     misc_feature    2282..2284
                     /label=F21-6.24
     misc_feature    2285..2287
                     /label=F22
     misc_feature    2291..2293
                     /label=F2
     misc_feature    2303..2305
                     /label=F3
     misc_feature    2315..2317
                     /label=F4
     primer_bind     complement(2663..2693)
                     /label=R-IRES-Nhel-gb-OXTR
     primer_bind     2669..2684
                     /label=F-IRES
     primer_bind     3229..3277
                     /label=F-IRES-NcoI-gb-mcherry
     primer_bind     complement(3233..3253)
                     /label=R-IRES
     gene            3260..3964
                     /label=mcherry
     primer_bind     complement(3946..4004)
                     /label=R-CAAX-gb-mcherry
     primer_bind     3980..4036
                     /label=F-CAAX-gb-pdisplayJM
     primer_bind     complement(3988..4037)
                     /label=R-pdisplay-gb-CAAX
     primer_bind     complement(4013..4037)
                     /label=overlap-pdisplayJM TAA
     primer_bind     4013..4036
                     /label=F-pdisplayJM
     misc_feature    4052..4061
                     /label=PDGFR transmembrane domain
                     /label=PDGFR transmembrane domain
     misc_feature    complement(4222..4239)
                     /label=BGH_rev_primer
                     /label=BGH_rev_primer
                     /label=BGH_rev_primer
                     /label=BGH_rev_primer
                     /label=BGH_rev_primer
                     /label=BGH_rev_primer
                     /label=BGH_rev_primer
                     /note="/translation=METDTLLLWVLLLWVPGSTGDYPYDVPDYAGAQPARSMK
                     DNTVP 
                     LKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQ 
                     LLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKW 
                     FSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQD 
                     RKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLA 
                     AMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGA 
                     LLLEQDGIIKPWMGGEISLRSAEKGSTSGSGGSKDEL*"
     terminator      4225..4452
                     /label=bGH_PA_terminator
                     /label=bGH_PA_terminator
                     /label=bGH_PA_terminator
                     /label=bGH_PA_terminator
                     /label=bGH_PA_terminator
                     /label=bGH_PA_terminator
                     /label=bGH_PA_terminator
                     /note="/translation=METDTLLLWVLLLWVPGSTGDYPYDVPDYAGAQPARSMK
                     DNTVP 
                     LKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQ 
                     LLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKW 
                     FSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQD 
                     RKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLA 
                     AMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGA 
                     LLLEQDGIIKPWMGGEISLRSAEKGSTSGSGGSKDEL*"
     rep_origin      complement(4569..5188)
                     /direction=LEFT
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /note="/translation=METDTLLLWVLLLWVPGSTGDYPYDVPDYAGAQPARSMK
                     DNTVP 
                     LKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQ 
                     LLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKW 
                     FSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQD 
                     RKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLA 
                     AMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGA 
                     LLLEQDGIIKPWMGGEISLRSAEKGSTSGSGGSKDEL*"
     primer_bind     4643..4660
                     /label=f-xa.7
     primer_bind     complement(4645..4662)
                     /label=f-xa.J
     primer_bind     complement(5111..5128)
                     /label=f-xa.G
     terminator      complement(5336..5605)
                     /label=TK_PA_terminator
                     /label=TK_PA_terminator
                     /label=TK_PA_terminator
                     /label=TK_PA_terminator
                     /label=TK_PA_terminator
                     /label=TK_PA_terminator
                     /label=TK_PA_terminator
                     /note="/translation=METDTLLLWVLLLWVPGSTGDYPYDVPDYAGAQPARSMK
                     DNTVP 
                     LKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQ 
                     LLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKW 
                     FSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQD 
                     RKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLA 
                     AMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGA 
                     LLLEQDGIIKPWMGGEISLRSAEKGSTSGSGGSKDEL*"
     CDS             5729..6259
                     /codon_start=1
                     /label=ORF frame 2
                     /label=ORF frame 2
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEAV
                     SPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILAVG
                     HARLEPGEQFGWREPLMLLIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQPPHCI
                     SHDGYFLGRSKVR"
     CDS             complement(5780..6568)
                     /codon_start=1
                     /label=ORF frame 3
                     /label=ORF frame 3
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEHQGL
                     APAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALA
                     TRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    complement(6674..6693)
                     /label=SV40pro_F_primer
                     /label=SV40pro_F_primer
                     /label=SV40pro_F_primer
                     /label=SV40pro_F_primer
                     /label=SV40pro_F_primer
                     /label=SV40pro_F_primer
                     /label=SV40pro_F_primer
                     /note="/translation=MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRL
                     SAQGR 
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL 
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEH 
                     QGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD 
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     rep_origin      complement(6678..6755)
                     /direction=LEFT
                     /label=SV40_origin
                     /label=SV40_origin
                     /label=SV40_origin
                     /label=SV40_origin
                     /label=SV40_origin
                     /label=SV40_origin
                     /label=SV40_origin
                     /note="/translation=MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRL
                     SAQGR 
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL 
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEH 
                     QGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD 
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     misc_feature    6920..6940
                     /label=pBABE_3_primer
                     /label=pBABE_3_primer
                     /label=pBABE_3_primer
                     /label=pBABE_3_primer
                     /label=pBABE_3_primer
                     /label=pBABE_3_primer
                     /label=pBABE_3_primer
                     /note="/translation=MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRL
                     SAQGR 
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL 
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEH 
                     QGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD 
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            complement(7023..7883)
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /note="/translation=MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRL
                     SAQGR 
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL 
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEH 
                     QGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD 
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(7023..7883)
                     /codon_start=1
                     /label=ORF frame 2
                     /label=ORF frame 2
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7234..7251
                     /label=f-xa.11
     primer_bind     complement(7240..7257)
                     /label=fxaE
     primer_bind     complement(7683..7700)
                     /label=f-xa.C
     primer_bind     7859..7876
                     /label=pRLTK.R
     promoter        complement(7925..7953)
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     rep_origin      8032..8338
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     primer_bind     8114..8131
                     /label=f-xa.15
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg
      721 cttggggata tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt
      781 tccaggttcc actggtgaca caagtttgta caaaaaagtt ggcaccaccg gtgagggtgc
      841 gtttgcggct aactggagcg ctgaggcggt caacgggagc gcggcgccgc cgggaaccga
      901 gggcaatcgc actgccgggc cgccacagcg caacgaggcc ctggcgcggg tggaggtggc
      961 cgtgctgtgc ctcatcctgt tcctggcgct gagcggcaac gcgtgcgtgc tgctagcgct
     1021 gcgcaccacg cgccacaagc actcgcgcct cttcttcttc atgaagcacc tgagcatagc
     1081 cgacctggta gtggcggtgt tccaggtgct gccgcagctt ctgtgggaca tcacgttccg
     1141 cttctacggg cccgacctgc tgtgccgcct cgtcaagtac ctgcaggttg tgggcatgtt
     1201 cgcgtccacc tacctgctgc tgctcatgtc gctcgaccgc tgcctggcca tctgccagcc
     1261 gctgcgctcg ctgagccgcc gcaccgaccg cctggcggta ctcgtcacat ggctcggctg
     1321 cctggtggcc agcgcgccgc aggtgcacat cttctcgctg cgcgaggtgg ccgacggtgt
     1381 cttcgactgc tgggccgttt tcattcaacc ctgggggccc aaggcctaca tcacgtggat
     1441 cacgctcgcc gtctacattg tgcccgtcat cgtgcttgcc acctgctatg gccttatcag
     1501 cttcaagatc tggcagaatt tacggctcaa gacggcggga ggaaacgtct atatcaaggc
     1561 cgacaagcag aagaacggca tcaaggcgaa cttccacatc cgccacaaca tcgaggacgg
     1621 cggcgtgcag ctcgcctacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     1681 gctgcccgac aaccactacc tgagcgtgca gtccaaactt tcgaaagacc ccaacgagaa
     1741 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     1801 cgagctgtac aagggcggta ccggagggag catggtgaga aagggcgagg agctgttcac
     1861 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1921 gtccggcgag ggtgagggcg atgccaccga gggcaagctg accctgaagt tcatctgcac
     1981 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     2041 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2101 cgaaggctac atccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     2161 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     2221 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacaccg gagcagcagc
     2281 aaagctcatc tctaaggcca agatccgcac ggtcaagatg accttcatcg tcgtgctggc
     2341 cttcatcgtg tgctggacgc cattcttttt cgtgcagatg tggagtgtct gggatgccga
     2401 tgcgcccaag gaagcctcac ctttcatcat cgccatgctc ctggccagcc tcaacagctg
     2461 ctgcaacccc tggatctaca tgctcttcac gggccacctc ttccaagaac ttgtgcagcg
     2521 cttcctctgc tgctcattcc gccgcctgaa aggcagccgg cctggggaga caagcgtcag
     2581 caaaaagagc aactcgtcta cctttgtcct gagccagtac agctccagcc agagaagatg
     2641 ctcgcagcca tccacgctgt gagctagcgc ccctctccct cccccccccc taacgttact
     2701 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     2761 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     2821 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     2881 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     2941 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     3001 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     3061 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     3121 tgtatgggat ctgatctggg gcctcggtac acatgcttta catgtgttta gtcgaggtta
     3181 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     3241 taatatggcc acaaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     3301 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     3361 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     3421 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     3481 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     3541 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     3601 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     3661 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     3721 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     3781 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     3841 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     3901 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     3961 caaggatggt aaaaagaaga aaaagaagtc aaagacaaag tgtgtaatta tgtaagtcga
     4021 cgaacaaaaa ctcatctcag aagaggatct gaatgctgtg ggccaggaca cgcaggaggt
     4081 catcgtggtg ccacactcct tgccctttaa ggtggtggtg atctcagcca tcctggccct
     4141 ggtggtgctc accatcatct cccttatcat cctcatcatg cttggcagaa gaagccacgt
     4201 taggcggccg ctcgagatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     4261 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     4321 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     4381 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     4441 tgggctctat ggcttctgag gcggaaagaa ccagtggcgg taatacggtt atccacagaa
     4501 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4561 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4621 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4681 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4741 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4801 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4861 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4921 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4981 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     5041 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5101 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     5161 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     5221 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     5281 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaacc tgaggctatg
     5341 gcagggcctg ccgccccgac gttggctgcg agccctgggc cttcacccga acttgggggg
     5401 tggggtgggg aaaaggaaga aacgcgggcg tattggcccc aatggggtct cggtggggta
     5461 tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga cccaacaccg
     5521 tgcgttttat tctgtctttt tattgccgtc atagcgcggg ttccttccgg tattgtctcc
     5581 ttccgtgttt cagttagcct ccccctaggg tgggcgaaga actccagcat gagatccccg
     5641 cgctggagga tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag
     5701 aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt
     5761 tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct
     5821 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
     5881 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
     5941 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
     6001 aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgctcgcc ttgagcctgg
     6061 cgaacagttc ggctggcgcg agcccctgat gctcttgatc atcctgatcg acaagaccgg
     6121 cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg
     6181 tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg
     6241 caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt
     6301 cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca
     6361 gccacgatag ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac aggtcggtct
     6421 tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc
     6481 cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac
     6541 ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcga
     6601 tctttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
     6661 gccgaggagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     6721 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
     6781 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     6841 tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     6901 gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt ctttccgcct
     6961 caggactctt cctttttcaa taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7021 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7081 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7141 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     7201 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7261 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     7321 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     7381 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     7441 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7501 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7561 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7621 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     7681 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7741 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7801 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7861 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7921 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7981 gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc
     8041 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     8101 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     8161 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     8221 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     8281 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     8341 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     8401 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     8461 cttacaattt ac
//
