LOCUS       Exported                6305 bp DNA     circular SYN 13-APR-2022
DEFINITION  Sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 13, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     unsure          2..142
                     /label=L-ITR
     misc_feature    126..184
                     /label=73_F_AAV_MluI_EFS
     promoter        163..374
                     /label=EF-1-alpha core promoter
                     /label=EF-1-alpha core promoter
     misc_feature    complement(353..411)
                     /label=74_R_XbaI_EFS
     primer_bind     393..426
                     /label=Loxp
     primer_bind     complement(393..424)
                     /label=LoxP
     primer_bind     complement(393..424)
                     /label=Loxp(o)Æ
     primer_bind     398..428
                     /label=R_loxP_NheI_IgK
     misc_feature    477..510
                     /label=loxP
                     /label=loxP
                     /label=loxP
                     /label=loxP
                     /label=loxP
                     /label=loxP
                     /label=loxP
                     /label=loxP
                     /label=loxP
     primer_bind     477..510
                     /label=Loxp
     primer_bind     complement(477..508)
                     /label=LoxP
     primer_bind     complement(477..508)
                     /label=Loxp(o)Æ
     primer_bind     491..540
                     /label=F-loxP-NdeI-OXT1.0
     gene            521..2503
                     /label=DIO-GRAB-OXT1.0
     primer_bind     complement(2492..2544)
                     /label=R_loxP_NheI_IgK
     gene            complement(2504..2509)
                     /label=Kozak sequence
                     /label=Kozak sequence
     primer_bind     complement(2516..2549)
                     /label=Loxp
     primer_bind     2518..2549
                     /label=LoxP
     primer_bind     2518..2549
                     /label=Loxp(o)Æ
     primer_bind     complement(2600..2633)
                     /label=Loxp
     primer_bind     complement(2600..2619)
                     /label=F-loxP-NdeI-OXT1.0
     primer_bind     2602..2633
                     /label=LoxP
     primer_bind     2602..2633
                     /label=Loxp(o)Æ
     misc_feature    2660..3022
                     /label=WPRE
                     /label=WPRE
                     /label=WPRE
                     /label=WPRE
                     /label=WPRE
                     /label=WPRE
                     /label=WPRE
                     /label=WPRE
                     /label=WPRE
                     /note="/translation=MYAIRSYEFDIKLIDNQPLDYKICERLTGILNYVAPFTL
                     CGYAA 
                     LMPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATT 
                     CQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKSSSFPWLLAYVAT 
                     WILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPHRYRALL 
                     ERSTGGIPVTPPQCLSWPWKLPLQCPPALS*"
     terminator      3051..3529
                     /label=hGH_PA_terminator
                     /label=hGH_PA_terminator
                     /label=hGH_PA_terminator
                     /label=hGH_PA_terminator
                     /label=hGH_PA_terminator
                     /label=hGH_PA_terminator
                     /label=hGH_PA_terminator
                     /label=hGH_PA_terminator
                     /label=hGH_PA_terminator
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTERCSRDLRVASL*"
     unsure          3794..3934
                     /label=R-ITR
     rep_origin      3801..4107
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTERCSRDLRVASL*"
     misc_feature    4375..4397
                     /label=pGEX_3_primer
                     /label=pGEX_3_primer
                     /label=pGEX_3_primer
                     /label=pGEX_3_primer
                     /label=pGEX_3_primer
                     /label=pGEX_3_primer
                     /label=pGEX_3_primer
                     /label=pGEX_3_primer
                     /label=pGEX_3_primer
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTERCSRDLRVASL*"
     promoter        4556..4584
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTERCSRDLRVASL*"
     gene            4626..5486
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTERCSRDLRVASL*"
     CDS             4626..5486
                     /codon_start=1
                     /label=ORF frame 1
                     /label=ORF frame 1
                     /translation="MPSSPKRARRKGLVIRLFL*VNVMIIMVS*TSGGTFRGNVRGTPI
                     CLFF*IHSNMYPLMRQ*P**MLQ*Y*KRKSMSIQHFRVALIPFFAAFCLPVFAHPETLV
                     KVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQ
                     LGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELT
                     AFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLI
                     DWMEAD"
     rep_origin      5641..6260
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     misc_feature    5926..5943
                     /label=64-f-xa.G
ORIGIN
        1 tcctgcaggc agctgcgcgc tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt
       61 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc
      121 aactccatca ctaggggttc ctgcggccgc acgcgtaagc ttgggcagag cgcacatcgc
      181 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
      241 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
      301 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
      361 ccgccagaac acagtctaga gtcgactccg gaataacttc gtataggata ctttatacga
      421 agttatgcag aatggtagct ggattgtagc tgctattagc aatatgaaac ctcttaataa
      481 cttcgtatag catacattat acgaagttat ggcgcatatg tcacagcgtg gatggctgcg
      541 agcatcttct ctggctggag ctgtactggc tcaggacaaa ggtagacgag ttgctctttt
      601 tgctgacgct tgtctcccca ggccggctgc ctttcaggcg gcggaatgag cagcagagga
      661 agcgctgcac aagttcttgg aagaggtggc ccgtgaagag catgtagatc caggggttgc
      721 agcagctgtt gaggctggcc aggagcatgg cgatgatgaa aggtgaggct tccttgggcg
      781 catcggcatc ccagacactc cacatctgca cgaaaaagaa tggcgtccag cacacgatga
      841 aggccagcac gacgatgaag gtcatcttga ccgtgcggat cttggcctta gagatgagct
      901 tgttctgccg cccgcgccag cgtgctgctg ctccggtgtt gtactccagc ttgtgcccca
      961 ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt
     1021 cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg
     1081 tgcgctcctg gatgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt
     1141 ggtcggggta gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc
     1201 agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccctcggtgg
     1261 catcgccctc accctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct
     1321 cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc ctttctcacc atgctccctc
     1381 cggtaccgcc cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
     1441 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt cgaaagtttg gactgcacgc
     1501 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     1561 agtggtaggc gagctgcacg ccgccgtcct cgatgttgtg gcggatgtgg aagttcgcct
     1621 tgatgccgtt cttctgcttg tcggccttga tatagacgtt tcctccctcc tccaggtcca
     1681 gcgcgtcggt gtcgcgcggc cccgccgtct tgagccgtaa attctgccag atcttgaagc
     1741 tgataaggcc atagcaggtg gcaagcacga tgacgggcac aatgtagacg gcgagcgtga
     1801 tccacgtgat gtaggccttg ggcccccagg gttgaatgaa aacggcccag cagtcgaaga
     1861 caccgtcggc cacctcgcgc agcgagaaga tgtgcacctg cggcgcgctg gccaccaggc
     1921 agccgagcca tgtgacgagt accgccaggc ggtcggtgcg gcggctcagc gagcgcagcg
     1981 gctggcagat ggccaggcag cggtcgagcg acatgagcag cagcaggtag gtggacgcga
     2041 acatgcccac aacctgcagg tacttgacga ggcggcacag caggtcgggc ccgtagaagc
     2101 ggaacgtgat gtcccacaga agctgcggca gcacctggaa caccgccact accaggtcgg
     2161 ctatgctcag gtgcttcatg aagaagaaga ggcgcgagtg cttgtggcgc gtggtgcgca
     2221 gcgctagcag cacgcacgcg ttgccgctca gcgccaggaa caggatgagg cacagcacgg
     2281 ccacctccac ccgcgccagg gcctcgttgc gctgtggcgg cccggcagtg cgattgccct
     2341 cggttcccgg cggcgccgcg ctcccgttga ccgcctcagc gctccagtta gccgcaaacg
     2401 caccctcacc ggtggtgcca acttttttgt acaaacttgt gtcaccagtg gaacctggaa
     2461 cccagagcag cagtacccat agcaggagtg tgtctgtctc catggtggcg ctagcataac
     2521 ttcgtataaa gtatcctata cgaagttatt tgccttaacc cagaaattat cactgttatt
     2581 ctttagaatg gtgcaaagaa taacttcgta taatgtatgc tatacgaagt tatgaattcg
     2641 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     2701 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     2761 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     2821 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     2881 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     2941 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     3001 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     3061 cctttccttg gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct
     3121 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     3181 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     3241 ccccgcatcg ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc
     3301 cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat
     3361 aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag
     3421 gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta
     3481 ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg
     3541 ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac
     3601 caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct
     3661 ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt
     3721 acaggcgtga accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg
     3781 accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
     3841 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
     3901 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt
     3961 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
     4021 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     4081 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     4141 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     4201 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
     4261 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     4321 aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc
     4381 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     4441 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
     4501 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     4561 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     4621 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     4681 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     4741 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4801 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4861 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4921 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4981 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5041 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5101 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5161 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5221 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5281 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5341 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     5401 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5461 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5521 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5581 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5641 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     5701 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5761 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5821 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5881 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5941 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6001 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     6061 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6121 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6181 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6241 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6301 ggaga
//
