LOCUS       Exported                6421 bp DNA     circular SYN 13-APR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 13, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     unsure          1..141
                     /label=L-ITR
                     /note="/vntifkey=21"
     promoter        156..597
                     /label=synapsin
     primer_bind     569..617
                     /label=F_hSyn_NcoI_IgK
     primer_bind     572..597
                     /label=c3 dio fw 4
     CDS             598..2523
                     /codon_start=1
                     /label=OXTmut
                     /translation="METDTLLLWVLLLWVPGSTGDTSLYKKVGTTGEGAFAANWSAEAV
                     NGSAAPPGTEGNRTAGPPQRNEALARVEVAVLCLILFLALSGNACVLLALRTTRHKHSR
                     LFFFMKHLSIADLVVAVFQVLPQLLWDITFRFYGPDLLCRLVKYLQVVGMFASTYLLLL
                     MSLDRCLAICQPLRSLSRRTDRLAVLVTWLGCLVASAPQVHIFSLREVADGVFDCWAVF
                     IQPWGPKAYITWITLAVYIVPVIVLATCYGLISFKIWQNLRLKTAGGNVYIKADKQKNG
                     IKANFHIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYKGGTGGSMVRKGEELFTGVVPILVELDGDVNGHKFSVSGEG
                     EGDATEGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGY
                     IQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNTGAAAKL
                     ISKAKIRTVKMTFIVVLAFIVCWTPFFFVQMWSVWDADAPKEASPFIIAMLLASLNSCC
                     NPWIYMLFTGHLFQELVQRFLCCSFRRLKGSRPGETSVSKKSNSSTFVLSQYSSSQRRC
                     SQPSTL"
     misc_feature    598..660
                     /label=IgK
     misc_feature    1399..2142
                     /label=GG-cpEGFP-TGAAA
     primer_bind     complement(2524..2554)
                     /label=R_pAAV_EcoRI_OXT1.0
     unsure          2538..3146
                     /label=WPRE
                     /note="/vntifkey=21"
     primer_bind     complement(2623..2640)
                     /label=pWPTRev
     polyA_signal    3166..3644
                     /label=hGH polyA
                     /label=hGH polyA
                     /label=PolyA signal
                     /note="/vntifkey=25"
     unsure          3684..3824
                     /label=R-ITR
                     /note="/vntifkey=21"
     rep_origin      3916..4222
                     /label=f1 Origin
                     /label=f1 Origin
                     /label=Replication origin
                     /note="/vntifkey=33"
     unsure          4741..5598
                     /label=AmpR
                     /note="/vntifkey=21"
     rep_origin      5749..6416
                     /label=pUC ori
                     /label=pUC ori
                     /label=Replication origin
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtatgag gcggggtggg ggtgcctacc
      181 tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc
      241 ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt
      301 cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca
      361 ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg
      421 gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg
      481 ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg
      541 tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gagaaaccgg tgccaccatg
      601 gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc cactggtgac
      661 acaagtttgt acaaaaaagt tggcaccacc ggtgagggtg cgtttgcggc taactggagc
      721 gctgaggcgg tcaacgggag cgcggcgccg ccgggaaccg agggcaatcg cactgccggg
      781 ccgccacagc gcaacgaggc cctggcgcgg gtggaggtgg ccgtgctgtg cctcatcctg
      841 ttcctggcgc tgagcggcaa cgcgtgcgtg ctgctagcgc tgcgcaccac gcgccacaag
      901 cactcgcgcc tcttcttctt catgaagcac ctgagcatag ccgacctggt agtggcggtg
      961 ttccaggtgc tgccgcagct tctgtgggac atcacgttcc gcttctacgg gcccgacctg
     1021 ctgtgccgcc tcgtcaagta cctgcaggtt gtgggcatgt tcgcgtccac ctacctgctg
     1081 ctgctcatgt cgctcgaccg ctgcctggcc atctgccagc cgctgcgctc gctgagccgc
     1141 cgcaccgacc gcctggcggt actcgtcaca tggctcggct gcctggtggc cagcgcgccg
     1201 caggtgcaca tcttctcgct gcgcgaggtg gccgacggtg tcttcgactg ctgggccgtt
     1261 ttcattcaac cctgggggcc caaggcctac atcacgtgga tcacgctcgc cgtctacatt
     1321 gtgcccgtca tcgtgcttgc cacctgctat ggccttatca gcttcaagat ctggcagaat
     1381 ttacggctca agacggcggg aggaaacgtc tatatcaagg ccgacaagca gaagaacggc
     1441 atcaaggcga acttccacat ccgccacaac atcgaggacg gcggcgtgca gctcgcctac
     1501 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1561 ctgagcgtgc agtccaaact ttcgaaagac cccaacgaga agcgcgatca catggtcctg
     1621 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggcggt
     1681 accggaggga gcatggtgag aaagggcgag gagctgttca ccggggtggt gcccatcctg
     1741 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggtgagggc
     1801 gatgccaccg agggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     1861 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     1921 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta catccaggag
     1981 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     2041 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     2101 atcctggggc acaagctgga gtacaacacc ggagcagcag caaagctcat ctctaaggcc
     2161 aagatccgca cggtcaagat gaccttcatc gtcgtgctgg ccttcatcgt gtgctggacg
     2221 ccattctttt tcgtgcagat gtggagtgtc tgggatgccg atgcgcccaa ggaagcctca
     2281 cctttcatca tcgccatgct cctggccagc ctcaacagct gctgcaaccc ctggatctac
     2341 atgctcttca cgggccacct cttccaagaa cttgtgcagc gcttcctctg ctgctcattc
     2401 cgccgcctga aaggcagccg gcctggggag acaagcgtca gcaaaaagag caactcgtct
     2461 acctttgtcc tgagccagta cagctccagc cagagaagat gctcgcagcc atccacgctg
     2521 tgagaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     2581 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     2641 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     2701 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     2761 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     2821 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     2881 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     2941 aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct gcgcgggacg
     3001 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     3061 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     3121 tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctacggg tggcatccct
     3181 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc
     3241 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta
     3301 tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct
     3361 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct
     3421 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg
     3481 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat
     3541 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat
     3601 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt ttgtaggtaa
     3661 ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     3721 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     3781 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     3841 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     3901 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3961 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4021 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4081 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     4141 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4201 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
     4261 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4321 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     4381 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     4441 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     4501 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     4561 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     4621 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4681 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4741 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4801 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4861 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4921 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4981 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5041 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5101 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5161 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5221 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5281 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5341 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5401 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5461 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5521 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5581 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5641 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5701 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5761 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5821 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5881 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     5941 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6001 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6061 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6121 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6181 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6241 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6301 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6361 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6421 t
//
