LOCUS       RS17060_pMQ30_KO_Kan        7159 bp ds-DNA     circular     29-JAN-2020
DEFINITION  .
ACCESSION   pMQ30
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     gene            <1..403
                     /locus_tag="RR42_RS17055"
                     /old_locus_tag="RR42_m3472"
     misc_feature    1..500
                     /standard_name="RS17060_upHomology"
     source          <1..>500
                     /organism="Cupriavidus basilensis"
                     /mol_type="genomic DNA"
                     /strain="4G11"
                     /isolation_source="Ground water"
                     /db_xref="taxon:68895"
                     /chromosome="main"
                     /country="USA"
                     /lat_lon="35.98 N 84.27 W"
                     /collection_date="26-Nov-2012"
     CDS             <1..403
                     /codon_start=2
                     /locus_tag="RR42_RS17055"
                     /old_locus_tag="RR42_m3472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="WP_043349302.1"
                     /db_xref="GI:759631588"
                     /translation="SAEATPEIVGRARALAACGFVGKHLDANAFTAAVRKVLAGDTSF
                     PSDEALNGRAQSFGPAHGIPVTPAELGLTPRQGSVLALVLEGLPNKVIARRLGLTENT
                     VKEHVSAILQRLGVRTRMQVMSRMERFRLRQ"
     misc_feature    1..1000
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="TAATGACAGGGCGCGGGGCGGGCGCCCGCCCACGCGTTTTTG
                     CACAAAACTATGCCACCAGGCCCCGTGCGGGCTATGGTGAGCCTGCCAGGCAATGCCT
                     GCGTGTGAGGTAAGGCTGTGTACGATTTCATCATCATTGGCGGCGGGATTGTGGGCAT
                     GTCCACGGCCATGCACCTGATCAAGGTCTACCCGGACGCGAAGATGCTCCTGCTGGAG
                     AAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCGGCGTGATCCACGCCG
                     GCGTGTATTACACCCCCGGCAGCCTCAAGGCGCGCTTCTGCCTGGAAGGCAACAAGGC
                     GACCAAAGCCTTCTGCACCCAGCACGGCATTCGCTTCGATGAGTGCGGCAAGCTGCTG
                     GTAGCCACCAACGATCTGGAAATGCAGCGCATGAAGGCGCTGTGGGAGCGGACGGCGG
                     CCAATGGCCTGGAACGCTACTGGCTGTCGGCCGACGAGTTGCGCGAGCGTGAGCCCAA
                     CATCGTCGGTATGGGGGGCATCTTCGTGCCGTCCAGCGGTATCGTCAATTACGCCCAG
                     GTGACGGCTGCGATGGCCGCTGAGTTCCAGCGTGCCGGTGGCGAAATCCGCTATGGCG
                     CAGAAGTGGTCGGCCTGCAGGAACAGGCAAATGAAGTGATCGTGCGCACCCAGCGTGA
                     TGAACTGCACAGCCGTTTCCTGGTGACCTGCTCGGGGCTGATGGCTGACCGCGTGGTG
                     GGCATGCTCGGGCTGCGTACCGAGTTCGTGATCTGCCCGTTCCGTGGCGAATACTACC
                     TGCTGCCCAAGCAGCACAATCAGATCGTCAACCACCTGATCTACCCGATTCCCGACCC
                     GTCGATGCCCTTCCTGGGGGTGCACCTGACCCGCATGATCGATGGCACCGTCACCGTA
                     GGCCCCAACGCTGTGCTGGCGATGAAGCGCGAGGGCTACCGCAAGACCGATGTCAGCC
                     CCAGCGACCTGTTCCAGACCCTGACCACGCCCGGCATCCTCAAGGTGCTGGCGAAGAA
                     CTTCCGCCCGGGCCTGATCGAGATGAAGAACTCGCTGTTCAAGGGCGGGTACCTCAAG
                     CAGGTGCAGAAGTACTGCCCGAGCATTATCAAAGCCGACCTCACGCCTTACCCGGCCG
                     GCGTGCGTGCCCAGGCGGTGTCGCGCGACGGCAAGCTGATCGACGATTTCCTGTTCGT
                     CAACACCGCACGCAGTGTCAACGTGTGCAACGCACCGTCGCCAGCTGCTACCTCGGCC
                     ATCCCGATCGGCGCCTACATCGTCGAGAAGGTGTGCGAGCAGGTCGGCCTGCAGGGCG
                     GCAGCTTCCCCAAGGCCGACCTGTCGGCCAACCAGAGGGCTGCCAGCTGACAGCCGGG
                     TAACACCTTCTGCGGTTTCGTTTTACTCAACCCGTACTGCCCTGCTCACCATTCAGGG
                     CAGGGCGGGCTTTTTCGCGCCTGCGTCGGTGACGTGCAGCCGGCGGCCCCTGCGTGGG
                     CATGACAAAAACAACAAGGTAGCGCCATGCAAACCCACAAGAACAATTTGAGTCATGG
                     ATTGAAATCACGGCATGTCACCATGCTGTCCATTGCCGGCGTGATCGGCGCCGGGCTG
                     TTTGTCGGCTCTGGCCGCGCCATCGCCGAAGCGGGGCCTGCCACCATCCTGGCCTATA
                     TCCTGGCGGGCGGCCTGGTGGTGCTGGTGATGCGCATGCTGGCTGAAATGGCTGTGGC
                     CTCCCCGGACACCGGCTCGTTTTCAACCTACGCTGACCTCGCCATCGGCAAATGGGCC
                     GGTTACACCATCGGTTGGCTGTACTGGTGGTTCTGGGTGCTGATCATTCCGATCGAGG
                     CCAATATCGCCGCTACCATCATCAACTCCTGGGTCCCGCAGCTGGAGATCTGGGTGTT
                     GTCGTTGGTGATCACGTTGCTGCTGACGGCCACCAACCTGTTCAGCGTGAAGAATTAC
                     GGTGAGTTCGAGTTTTGGCTGGCACTGGTCAAGGTGGTGGCCATCGT"
     misc_feature    501..1000
                     /standard_name="RS17060_downHomology"
     source          <501..>1000
                     /organism="Cupriavidus basilensis"
                     /mol_type="genomic DNA"
                     /strain="4G11"
                     /isolation_source="Ground water"
                     /db_xref="taxon:68895"
                     /chromosome="main"
                     /country="USA"
                     /lat_lon="35.98 N 84.27 W"
                     /collection_date="26-Nov-2012"
     gene            689..>1000
                     /locus_tag="RR42_RS17065"
                     /old_locus_tag="RR42_m3474"
     CDS             689..>1000
                     /locus_tag="RR42_RS17065"
                     /old_locus_tag="RR42_m3474"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="WP_043352294.1"
                     /db_xref="GI:759634580"
                     /translation="VKVFRGLPNAESRAPCALTIGNFDGVHRGHQSLLARARAAADAR
                     GLPLCVMTFEPHPREFFTPDKAPTRIALLRDKLESLRRNGVDRVVVEHFNAHFAAQSP
                     QA"
     source          1001..7159
     CDS             complement(1275..2696)
                     /standard_name="sacB"
     oriT            3170..3994
                     /standard_name="conjugal transfer origin from pEX18-Gm"
     CDS             complement(4407..4691)
                     /standard_name="lacZ alpha"
     rep_origin      5066..5666
     source          <5930..>6724
     gene            complement(5930..6724)
                     /gene="nptII"
     source          complement(<5930..>6724)
                     /organism="Cloning vector pMQ150"
                     /mol_type="other DNA"
                     /db_xref="taxon:515316"
                     /note="shuttle vector"
     CDS             complement(5930..6724)
                     /gene="nptII"
                     /note="confers resistance to kanamycin; KanR"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 3'-phosphotransferase"
                     /protein_id="ACB45218.1"
                     /db_xref="GI:171194035"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    5931..6721
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="TAGGTGGCGGTACTTGGGTCGATATCAAAGTGCATCACTTCT
                     TCCCGTATGCCCAACTTTGTATAGAGAGCCACTGCGGGATCGTCACCGTAATCTGCTT
                     GCACGTAGATCACATAAGCACCAAGCGCGTTGGCCTCATGCTTGAGGAGATTGATGAG
                     CGCGGTGGCAATGCCCTGCCTCCGGTGCTCGCCGGAGACTGCGAGATCATAGATATAG
                     ATCTCACTACGCGGCTGCTCAAACCTGGGCAGAACGTAAGCCGCGAGAGCGCCAACAA
                     CCGCTTCTTGGTCGAAGGCAGCAAGCGCGATGAATGTCTTACTACGGAGCAAGTTCCC
                     GAGGTAATCGGAGTCCGGCTGATGTTGGGAGTAGGTGGCTACGTCTCCGAACTCACGA
                     CCGAAAAGATCAAGAGCAGCCCGCATGGATTTGACTTGGTCAGGGCCGAGCCTACATG
                     TGCGAATGATGCCCATACTTGAGCCACCTAACTTTGTTTTAGGGCGACTGCCCTGCTG
                     CGTAACATCGTTGCTGCTGCGTAA"
     misc_feature    complement(6096..6115)
                     /standard_name="Rev_KanScreen "
     misc_feature    7159^7160
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="ATGCGTGGGTCGTCGGCATCCAGGCAGCGTTCCAGCTCATGG
                     ATCTTGCCATCCAGTCTGAGCTCTGGGGGCAGGGCTCGCACGGTGCCGCCAATCGGGT
                     TGAGCTGGCTATCGAACAGGCCGTAGGCGTCAAAGCTGCGCTCCTCGAAGGCCTGGCT
                     GGCGATCAGGGCGTCGTCCAGTTGCTTGCCGCTCATGTGGGCGAACAGGTGCTGGCGC
                     TGCAGCATGGAATGACGGGTGAGTTTGTTCAGGTAGCTGGAGACCTCGAAGTACAGCA
                     CCCCCATGAGGATGCTGCTCCAGGCCACGAACAGAAAGCTGTACAGCGCCAGCAGGCG
                     GCTGGTAGAAGAACTCCAGCCTTTAGACGGGTTCAGCAATGGCATAGCCGGAGCCCCG
                     TACCGTACGGATCAGCGGCGACTGGCCGGGTGAGTCGATCTTCTTGCGCAGGCGGCCG
                     ATGTGCACGTCGATCAGGTTGGTGCCCGGATCGAAGTGATAGCCCCAGACTTCCTCGA
                     AAATCATCATCCGCGTGATCACCTGGCCGCTGTGGCGCATCAGGTACTCCAGCAGCTT
                     GTACTCGGTGGGCAACAGGTTGAGGGTGTTGTCACCGCGGCGCGCCTCGTGGCTGATC
                     AGGTCCAGCTGCAGATCGGCAACCTGCAAGCGGGTCTGGGTCGTGGGTACGCTGTTAC
                     GGCGCAGCAATACCTCGACTCGCGCGGCCATTTCGTCGGAGGCGAACGGCTTGGTCAG
                     GTAGTCGTCTCCGCCGGCACGCAGGCCGCGCACCCGCTCATCGACGTCGGAGAGGGCG
                     CTGATCATCAGGATCGGCGTAGCGATCTTGAGGTTGCGCAGGGTGGTGACGATGGTCA
                     GGCCGTCGACCTCGGGCAGCATGCGGTCCAGGGTGATCAGGTCGTAGCCGCCCGCAAT
                     GGCTTTGGCCAGGCCTTCACGGCCATTGTCGGCCCAATCCACTTCCAGACCGTGGCTG
                     GTCAGTTCGGCGACGATTTCCTGGCCGGTGACGGCGTCGTCTTCGATGGTCAGTACGC
                     GAGGCATGCTGGTTCCTGGGCATCTAATGGAGATCTGATTGTGCCAAAGAATAGACAA
                     CCGGCGATTTAAGAAAAATTCATCTAGCGGGCACCGATGCCTTCGGCGTGCATGATCT
                     GCGCAATGATCGGTACCGGTGTCATCAGGTGCGAGCGCATCATGGCGCTGGCGGTTTT
                     GGCATCCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCC
                     AGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCT
                     GGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAGGCAAT
                     GGCAGTATGGAACGCCTTGTGCCGCTCGTCCCACACATCCAGCCGCTGCTCGCGGGTT
                     TTTACTTCCACCACCTTGGCCAATGTATGCGAGTGGGCCAGCACCGATGCCTCCCAGG
                     CGTCATCGCCGCGCTCGATGGCCAAGGCAATGATCATCGCTTCCAGGTTGGCGCGGGC
                     GTCGTAGATGTCGTTCATCTCATCCAGCGACATGGGCGCTACCCGGTAGCCTTTCTGG
                     CTGATCGCGTTGACCAGGTGCTCGGCCACCAGTTGCGACAGTGCTTCGCGCAGCGGGC
                     CCACACCCAGGTCGTAGCGTTCCTTGAGGGTGCTCATCAACAGCTTTTCACCGGGCTT
                     GAACACCCCGCGGATGATGTCCTTCTTGAGCCTCTCATACCCGCTGAATGCTGAGTTT
                     TGTCGGGGGGCGAGCGCTTCCAAGATCCTGTTCCTCAAAACGTTTTGCCGATCATACC
                     GAAACCCTCTGCTGACGAAAAACAATGGACAAACACGAGTAGTGTCGTTGAAAAATAA
                     AAATGTAGACATTTTCAGTTAGTGGCGATATTTTTACCTCGCCCGCACTGATCCAGGT
                     CATGACCGGACGGCGCCATCTACTTTTTTAGCCAGGACACTGCCATG"
ORIGIN      
        1 gtcggccgag gccacgccgg aaatcgtggg ccgcgcgcgc gcgcttgccg cctgtggctt
       61 cgtcggcaag cacctggacg ccaacgcatt caccgccgcc gtgcgcaagg tgctggcagg
      121 cgacacctcc ttcccgtcgg acgaagcgct caatggccgt gctcaatcct tcgggccggc
      181 gcacggcatt ccggtcacgc cggcggaact cggcctgacc ccgcgccagg gctcggtgct
      241 ggcgctggtg ctcgaaggcc tgcccaacaa ggtcatcgcg cggcggctcg gcctgacgga
      301 aaacaccgtc aaggaacatg tctcggccat cctccagcgg ctaggcgtgc gcacccgcat
      361 gcaggtcatg tcgcgcatgg agcgattccg cctgcgccag taacgcgcac cggcaacgcc
      421 agctgctccc tgggcagacg gcgaacaaaa aagatgggcc agcgtgtcat gcgccggccc
      481 attttttatg ccttcgccgc aggagggctg gggggcagcg agtgcaatcc gccatatcat
      541 gccgcctttt ggggcgcaac gccacggcga tgagtcgatc cgtgcacaaa aacgccagtc
      601 cgggcatgac ggccaccgcc gccgggccaa acttgtccac cattcgcctt ataatgctcg
      661 ctttatccaa acgcttgttg ccgtcctcgt gaaagtcttc cgtggcctgc ccaacgccga
      721 aagccgggcg ccttgcgccc tcaccatcgg caattttgac ggtgtgcacc gcggccacca
      781 gtcgctgctc gcgcgcgcgc gcgctgcggc ggatgcacgc gggctaccgc tatgcgtgat
      841 gacattcgag ccgcatccgc gcgaattctt cacgccggac aaggcgccaa cgcgcattgc
      901 gctgctgcgc gacaagctgg aaagcctgcg ccgcaacggt gtcgatcgtg ttgtcgttga
      961 gcacttcaac gcacactttg ccgcgcagtc cccgcaagca tctaagaaac cattattatc
     1021 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     1081 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     1141 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     1201 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca taatcggcat
     1261 tttcttttgc gtttttattt gttaactgtt aattgtcctt gttcaaggat gctgtctttg
     1321 acaacagatg ttttcttgcc tttgatgttc agcaggaagc taggcgcaaa cgttgattgt
     1381 ttgtctgcgt agaatcctct gtttgtcata tagcttgtaa tcacgacatt gtttcctttc
     1441 gcttgaggta cagcgaagtg tgagtaagta aaggttacat cgttaggatc aagatccatt
     1501 tttaacacaa ggccagtttt gttcagcggc ttgtatgggc cagttaaaga attagaaaca
     1561 taaccaagca tgtaaatatc gttagacgta atgccgtcaa tcgtcatttt tgatccgcgg
     1621 gagtcagtga acagatacca tttgccgttc attttaaaga cgttcgcgcg ttcaatttca
     1681 tctgttactg tgttagatgc aatcagcggt ttcatcactt ttttcagtgt gtaatcatcg
     1741 tttagctcaa tcataccgag agcgccgttt gctaactcag ccgtgcgttt tttatcgctt
     1801 tgcagaagtt tttgactttc ttgacggaag aatgatgtgc ttttgccata gtatgctttg
     1861 ttaaataaag attcttcgcc ttggtagcca tcttcagttc cagtgtttgc ttcaaatact
     1921 aagtatttgt ggcctttatc ttctacgtag tgaggatctc tcagcgtatg gttgtcgcct
     1981 gagctgtagt tgccttcatc gatgaactgc tgtacatttt gatacgtttt tccgtcaccg
     2041 tcaaagattg atttataatc ctctacaccg ttgatgttca aagagctgtc tgatgctgat
     2101 acgttaactt gtgcagttgt cagtgtttgt ttgccgtaat gtttaccgga gaaatcagtg
     2161 tagaataaac ggatttttcc gtcagatgta aatgtggctg aacctgacca ttcttgtgtt
     2221 tggtctttta ggatagaatc atttgcatcg aatttgtcgc tgtctttaaa gacgcggcca
     2281 gcgtttttcc agctgtcaat agaagtttcg ccgacttttt gatagaacat gtaaatcgat
     2341 gtgtcatccg catttttagg atctccggct aatgcaaaga cgatgtggta gccgtgatag
     2401 tttgcgacag tgccgtcagc gttttgtaat ggccagctgt cccaaacgtc caggcctttt
     2461 gcagaagaga tatttttaat tgtggacgaa tcgaactcag gaacttgata tttttcattt
     2521 ttttgctgtt cagggatttg cagcatatca tggcgtgtaa tatgggaaat gccgtatgtt
     2581 tccttatatg gcttttggtt cgtttctttc gcaaacgctt gagttgcgcc tcctgccagc
     2641 agtgcggtag taaaggttaa tactgttgct tgttttgcaa actttttgat gttcatcgtt
     2701 catgtctcct tttttatgta ctgtgttagc ggtctgcttc ttccagccct cctgtttgaa
     2761 gatggcaagt tagttacgca caataaaaaa agacctaaaa tatgtaaggg gtgacgccaa
     2821 agtatacact ttgcccttta cacattttag gtcttgcctg ctttatcagt aacaaacccg
     2881 cgcgatttac ttttcgacct cattctatta gactctcgtt tggattgcaa ctggtctatt
     2941 ttcctctttt gtttgataga aaatcataaa aggatttgca gactacgggc ctaaagaact
     3001 aaaaaatcta tctgtttctt ttcattctct gtatttttta tagtttctgt tgcatgggca
     3061 taaagttgcc tttttaatca caattcagaa aatatcataa tatctcattt cactaaataa
     3121 tagtgaacgg caggtatatg tgatgggtta aaaaggatcg atcctctagc tagagtcgat
     3181 cttcgccagc agggcgagga tcgtggcatc accgaaccgc gccgtgcgcg ggtcgtcggt
     3241 gagccagagt ttcagcaggc cgcccaggcg gcccaggtcg ccattgatgc gggccagctc
     3301 gcggacgtgc tcatagtcca cgacgcccgt gattttgtag ccctggccga cggccagcag
     3361 gtaggccgac aggctcatgc cggccgccgc cgccttttcc tcaatcgctc ttcgttcgtc
     3421 tggaaggcag tacaccttga taggtgggct gcccttcctg gttggcttgg tttcatcagc
     3481 catccgcttg ccctcatctg ttacgccggc ggtagccggc cagcctcgca gagcaggatt
     3541 cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc cgctcgcggg
     3601 tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag gaaagtctac
     3661 acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat
     3721 cgctataatg accccgaagc agggttatgc agcggaaaag cgctgcttcc ctgctgtttt
     3781 gtggaatatc taccgactgg aaacaggcaa atgcaggaaa ttactgaact gaggggacag
     3841 gcgagagacg atgccaaaga gctacaccga cgagctggcc gagtgggttg aatcccgcgc
     3901 ggccaagaag cgccggcgtg atgaggctgc ggttgcgttc ctggcggtga gggcggatgt
     3961 cgatatgcgt aaggagaaaa taccgcatca ggcgcatatt tgaatgtatt tagaaaaata
     4021 aacaaaaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttaat
     4081 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcgc
     4141 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     4201 acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctggc
     4261 agttccctac tctcgcatgg ggagacccca cactaccatc ggcgctacgg cgtttcactt
     4321 ctgagttcgg catggggtca ggtgggacca ccgcgctact gccgccaggc aaattctgtt
     4381 ttatcagacc gcttctgcgt tctgatttaa tctgtatcag gctgaaaatc ttctctcatc
     4441 cgccaaaaca gccaagctcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     4501 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     4561 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
     4621 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattcgta
     4681 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     4741 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     4801 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     4861 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     4921 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     4981 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     5041 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     5101 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     5161 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     5221 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     5281 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     5341 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     5401 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     5461 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     5521 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     5581 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     5641 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5701 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     5761 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     5821 tatatatgag taaacttggt ctgacaatcg atgcgaattg gccgcggcgt tgtgacaatt
     5881 taccgaacaa ctccgcggcc gggaagccga tctcggcttg aacgaattgt cagaagaact
     5941 cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca
     6001 cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg
     6061 ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc
     6121 ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct
     6181 cgccgtcggg catccgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat
     6241 gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct
     6301 cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc
     6361 gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga
     6421 gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt
     6481 cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt
     6541 cttggagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct
     6601 gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat
     6661 agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa
     6721 tcatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
     6781 gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa aaccgccact
     6841 gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc
     6901 tacttgcatt acagtttacg aaccgaacag gcttatgtca attcggttga atactcatac
     6961 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     7021 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     7081 tgggtaataa ctgatataat taaattgaag ctctaatttg tgagtttagt atacatgcat
     7141 ttacttataa tacagtttt
//
