LOCUS       zz_TEV_YBBR_TRF2_p1539  6723 bp ds-DNA     circular     18-APR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(603..634)
                     /label="LoxP"
                     /ApEinfo_revcolor=#ff081d
                     /ApEinfo_fwdcolor=#ff081d
     misc_recomb     604..637
                     /label="LoxP"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    641..666
                     /label="I-CeuI"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    902..1315
                     /label="ZZ"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     misc_feature    1316..1405
                     /label="Linker TEV"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1406..1438
                     /label="YBBR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             1406..1438
                     /label="Translation 1406-1438"
     misc_feature    1442..3064
                     /label="TRF2"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3070..3077
                     /label="mutiple cloning site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     polyA_signal    complement(3137..3328)
                     /label="SV40 late polyA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(3394..3416)
                     /label="T7R (T7 terminator primer)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     C_region        3452..3618
                     /label="Tn7L"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     enhancer        3619..4330
                     /label="1629"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(4339..5171)
                     /label="Ori"
                     /ApEinfo_revcolor=#ff081d
                     /ApEinfo_fwdcolor=#ff081d
     misc_feature    4520..4666
                     /label="pMB1 ori (from pUC19, no ROP)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(4559..4666)
                     /label="RNAI"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4668..5108
                     /label="pMB1 ori (from pUC19, no ROP)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     enhancer        5227..6191
                     /label="lef2-603"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6216..6414
                     /label="Tn7R"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(join(6504..6723,1..533))
                     /label="PromGenCDS"
                     /ApEinfo_revcolor=#827cb4
                     /ApEinfo_fwdcolor=#827cb4
ORIGIN
        1 tctcactacg cggctgctca aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg
       61 cttcttggtc gaaggcagca agcgcgatga atgtcttact acggagcaag ttcccgaggt
      121 aatcggagtc cggctgatgt tgggagtagg tggctacgtc tccgaactca cgaccgaaaa
      181 gatcaagagc agcccgcatg gatttgactt ggtcagggcc gagcctacat gtgcgaatga
      241 tgcccatact tgagccacct aactttgttt tagggcgact gccctgctgc gtaacatcgt
      301 tgctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc
      361 gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg
      421 aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt
      481 gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt caactgggtt
      541 cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc
      601 gccataactt cgtatagcat acattatacg aagttatctg taactataac ggtcctaagg
      661 tagcgagttt aaacactagt atcgattcgc gacctactcc ggaatattaa tagatcatgg
      721 agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt ttcgtaacag
      781 ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca ccatcgggcg
      841 cggatctcgg tccgaaaccA TGTCGTACTA CCATCACCAT CACCATCACc atcacggttc
      901 tATGTGCatg gcaggccttg cgcaacacga tgaagccgtg gacaacaaat tcaacaaaga
      961 acaacaaaac gcgttctatg agatcttaca tttacctaac ttaaacgaag aacaacgaaa
     1021 cgccttcatc caaagtttaa aagatgaccc aagccaaagc gctaaccttt tagcagaagc
     1081 taaaaagcta aatgatgctc aggcgccgaa agtagacaac aaattcaaca aagaacaaca
     1141 aaacgcgttc tatgagatct tacatttacc taacttaaac gaagaacaac gaaacgcctt
     1201 catccaaagt ttaaaagatg acccaagcca aagcgctaac cttttagcag aagctaaaaa
     1261 gctaaatgat gctcaggcgc cgaaagtaga cgcaaattcc gcggggaagt caaccgatta
     1321 tgatattcca actactgcta gcgagaacct atatttccag ggaGATTATG ATATCCCTAC
     1381 GACTGAGAAT CTTTATTTTC AGGGCGACTC ATTGGAGTTT ATCGCATCAA AGCTCGCAAT
     1441 Ggccgcggga gccgggacgg cgggccccgc ttccggcccg ggcgtcgtgc gtgacccagc
     1501 ggcgtcacag ccgaggaagc ggcccggccg ggagggcggg gagggcgcgc ggcgatcgga
     1561 cacgatggcg ggaggaggcg ggagtagcga cggcagcggg cgggcagctg gcaggcgggc
     1621 gtcccgcagt agcgggcggg cccggcgggg gcgccacgag ccggggctgg ggggcccggc
     1681 ggagcgcggc gcgggggagg cacggctgga agaggcagtc aatcgctggg tgctcaagtt
     1741 ctacttccac gaggcgctgc gggcctttcg gggtagccgg tacggggact tcagacagat
     1801 ccgggacatc atgcaggctt tgcttgtcag gcccttgggg aaggagcaca ccgtgtcccg
     1861 attgctgcgg gttatgcagt gtctgtcgcg gattgaagaa ggggaaaatt tagactgttc
     1921 ctttgatatg gaggctgagc tcacaccact ggaatcagct atcaatgtgc tggagatgat
     1981 taaaacggaa tttacactga cagaagcagt ggtcgaatcc agtagaaaac tggtcaagga
     2041 agctgctgtc attatttgta tcaaaaacaa agaatttgaa aaggcttcaa aaattttgaa
     2101 aaaacatatg tccaaggacc ccacaactca gaagctgaga aatgatctcc tgaatattat
     2161 tcgagaaaag aacttggccc atcctgttat ccagaacttt tcatatgaga ccttccagca
     2221 gaagatgctg cgcttcctgg agagccacct ggatgacgcc gagccctacc tcctcacgat
     2281 ggccaaaaag gctttgaaat ctgagtccgc tgcctcaagt acagggaagg aagataaaca
     2341 gccagcacca gggcctgtgg aaaagccacc cagagaaccc gcaaggcagc tacggaatcc
     2401 tccaaccacc attggaatga tgactctgaa agcagctttc aagactctgt ctggtgcaca
     2461 ggattctgag gcagcctttg caaaactgga ccagaaggat ctggttcttc ctactcaagc
     2521 tctcccagca tcaccagccc tcaaaaacaa gagacccaga aaagatgaaa acgaaagttc
     2581 agccccggct gacggtgagg gtggctcgga actgcagccc aagaacaagc gcatgacaat
     2641 aagcagattg gtcttggagg aggacagcca gagtactgag cccagcgcag gcctcaactc
     2701 ctcccaggag gccgcttcag cgccaccatc caagcccacc gttctcaacc aacccctccc
     2761 tggagagaag aatcccaaag tacccaaagg caagtggaac agctctaatg gggttgaaga
     2821 aaaggagact tgggtggaag aggatgaact gtttcaagtt caggcaccag atgaagacag
     2881 tacaaccaat ataacaaaaa agcagaagtg gactgtagaa gaaagcgagt gggtcaaggc
     2941 tggagtgcag aaatatgggg aaggaaactg ggctgccatt tctaaaaatt acccatttgt
     3001 taaccgaaca gctgtgatga ttaaggatcg ctggcggacc atgaaaagac ttggcatgaa
     3061 ctgataatga gcttgtcgag aagtactaga ggatcataat cagccatacc acatttgtag
     3121 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     3181 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     3241 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     3301 aactcatcaa tgtatcttat catgtctgga tctgatcact gcttgagcct agaagatccg
     3361 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
     3421 tcataacccc tagggtatac ccatctaatt ggaaccagat aagtgaaatc tagttccaaa
     3481 ctattttgtc atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat
     3541 tttgtcactc ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa
     3601 ctattttgtc cgcccacaac cggtggagga aattctcctt gaagtttccc tggtgttcaa
     3661 agtaaaggag tttgcaccag acgcacctct gttcactggt ccggcgtatt aaaacacgat
     3721 acattgttat tagtacattt attaagcgct agattctgtg cgttgttgat ttacagacaa
     3781 ttgttgtacg tattttaata attcattaaa tttataatct ttagggtggt atgttagagc
     3841 gaaaatcaaa tgattttcag cgtctttata tctgaattta aatattaaat cctcaataga
     3901 tttgtaaaat aggtttcgat tagtttcaaa caagggttgt ttttccgaac cgatggctgg
     3961 actatctaat ggattttcgc tcaacgccac aaaacttgcc aaatcttgta gcagcaatct
     4021 agctttgtcg atattcgttt gtgttttgtt ttgtaataaa ggttcgacgt cgttcaaaat
     4081 attatgcgct tttgtatttc tttcatcact gtcgttagtg tacaattgac tcgacgtaaa
     4141 cacgttaaat agagcttgga catatttaac atcgggcgtg ttagctttat taggccgatt
     4201 atcgtcgtcg tcccaaccct cgtcgttaga agttgcttcc gaagacgatt ttgccatagc
     4261 cacacgacgc ctattaattg tgtcggctaa cacgtccgcg atcaaatttg tagttgagct
     4321 ttttggaatt accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt
     4381 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     4441 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     4501 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4561 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4621 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     4681 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     4741 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     4801 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     4861 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     4921 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     4981 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5041 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5101 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5161 ctcacatgtt ctttcctgcg ttatcccctg attgacttgg gtcgctcttc ctgtggatgc
     5221 gcaggtatgt acaggaagag gtttatacta aactgttaca ttgcaaacgt ggtttcgtgt
     5281 gccaagtgtg aaaaccgatg tttaatcaag gctctgacgc atttctacaa ccacgactct
     5341 aagtgtgtgg gtgaagtcat gcatctttta atcaaatccc aagatgtgta taaaccacca
     5401 aactgccaaa aaatgaaaac tgtcgacaag ctctgtccgt ttgctggcaa ctgcaagggt
     5461 ctcaatccta tttgtaatta ttgaataata aaacaattat aaatgtcaaa tttgtttttt
     5521 attaacgata caaaccaaac gcaacaagaa catttgtagt attatctata attgaaaacg
     5581 cgtagttata atcgctgagg taatatttaa aatcattttc aaatgattca cagttaattt
     5641 gcgacaatat aattttattt tcacataaac tagacgcctt gtcgtcttct tcttcgtatt
     5701 ccttctcttt ttcatttttc tcttcataaa aattaacata gttattatcg tatccatata
     5761 tgtatctatc gtatagagta aattttttgt tgtcataaat atatatgtct tttttaatgg
     5821 ggtgtatagt accgctgcgc atagtttttc tgtaatttac aacagtgcta ttttctggta
     5881 gttcttcgga gtgtgttgct ttaattatta aatttatata atcaatgaat ttgggatcgt
     5941 cggttttgta caatatgttg ccggcatagt acgcagcttc ttctagttca attacaccat
     6001 tttttagcag caccggatta acataacttt ccaaaatgtt gtacgaaccg ttaaacaaaa
     6061 acagttcacc tcccttttct atactattgt ctgcgagcag ttgtttgttg ttaaaaataa
     6121 cagccattgt aatgagacgc acaaactaat atcacaaact ggaaatgtct atcaatatat
     6181 agttgctgat tgcgcagatg ccctgcgtaa gcgggtgtgg gcggacaata aagtcttaaa
     6241 ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa actagacaga atagttgtaa
     6301 actgaaatca gtccagttat gctgtgaaaa agcatactgg acttttgtta tggctaaagc
     6361 aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt aaagaggggc gtggccaagg
     6421 gcatgtaaag actatattcg cggcgttgtg acaatttacc gaacaactcc gcggccggga
     6481 agccgatctc ggcttgaacg aattgttagg tggcggtact tgggtcgata tcaaagtgca
     6541 tcacttcttc ccgtatgccc aactttgtat agagagccac tgcgggatcg tcaccgtaat
     6601 ctgcttgcac gtagatcaca taagcaccaa gcgcgttggc ctcatgcttg aggagattga
     6661 tgagcgcggt ggcaatgccc tgcctccggt gctcgccgga gactgcgaga tcatagatat
     6721 aga
//