LOCUS       zz_TEV_YBBR_POT1_p1539  6999 bp ds-DNA     circular     18-APR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(138..970)
                     /label="Ori"
                     /ApEinfo_revcolor=#ff081d
                     /ApEinfo_fwdcolor=#ff081d
     misc_feature    319..465
                     /label="pMB1 ori (from pUC19, no ROP)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(358..465)
                     /label="RNAI"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    467..907
                     /label="pMB1 ori (from pUC19, no ROP)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     enhancer        1026..1990
                     /label="lef2-603"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2015..2213
                     /label="Tn7R"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(2303..3055)
                     /label="PromGenCDS"
                     /ApEinfo_revcolor=#827cb4
                     /ApEinfo_fwdcolor=#827cb4
     misc_feature    complement(3125..3156)
                     /label="LoxP"
                     /ApEinfo_revcolor=#ff081d
                     /ApEinfo_fwdcolor=#ff081d
     misc_recomb     3126..3159
                     /label="LoxP"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    3163..3188
                     /label="I-CeuI"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3424..3837
                     /label="ZZ"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     misc_feature    3838..3927
                     /label="Linker TEV"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(3906..3927)
                     /label="p1539 ZZ-LTL-BICD2N-1_400-GFP REV"
                     /note="sequence: gttgctggaaccaaagacatGCCCTGAAAATAAAGATTCTCA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3931..3963
                     /label="YBBR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             3931..3963
                     /label="Translation 3931-3963"
     primer          3964..3983
                     /label="POT1 (POT1, BC002923, clone 3955573) FWD"
                     /note="sequence: AGAATCTTTATTTTCAGGGCatgtctttggttccagcaac"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3964..5868
                     /label="POT1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(5846..5868)
                     /label="POT1 (POT1, BC002923, clone 3955573) REV"
                     /note="sequence: tctagtacttctcgacaagcttagattacatcttctgcaactg"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5869..5875
                     /label="mutiple cloning site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          5869..5888
                     /label="p1539 ZZ-LTL-BICD2N-1_400-GFP FWD"
                     /note="sequence: ttgcagaagatgtaatctaagcttgtcgagaagtactaga"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     polyA_signal    complement(5935..6126)
                     /label="SV40 late polyA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(6192..6214)
                     /label="T7R (T7 terminator primer)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     C_region        6250..6416
                     /label="Tn7L"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     enhancer        join(6417..6999,1..129)
                     /label="1629"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 tcgtcgtcgt cccaaccctc gtcgttagaa gttgcttccg aagacgattt tgccatagcc
       61 acacgacgcc tattaattgt gtcggctaac acgtccgcga tcaaatttgt agttgagctt
      121 tttggaatta ccggttgact tgggtcaact gtcagaccaa gtttactcat atatacttta
      181 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
      241 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
      301 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
      361 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
      421 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      481 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      541 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      601 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      661 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      721 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      781 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      841 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      901 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      961 tcacatgttc tttcctgcgt tatcccctga ttgacttggg tcgctcttcc tgtggatgcg
     1021 caggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg
     1081 ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcta
     1141 agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa
     1201 actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc
     1261 tcaatcctat ttgtaattat tgaataataa aacaattata aatgtcaaat ttgtttttta
     1321 ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc
     1381 gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg
     1441 cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc
     1501 cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt atccatatat
     1561 gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg
     1621 gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag
     1681 ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc
     1741 ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt
     1801 ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa
     1861 cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac
     1921 agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata
     1981 gttgctgatt gcgcagatgc cctgcgtaag cgggtgtggg cggacaataa agtcttaaac
     2041 tgaacaaaat agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa
     2101 ctgaaatcag tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca
     2161 aactcttcat tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg
     2221 catgtaaaga ctatattcgc ggcgttgtga caatttaccg aacaactccg cggccgggaa
     2281 gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatat caaagtgcat
     2341 cacttcttcc cgtatgccca actttgtata gagagccact gcgggatcgt caccgtaatc
     2401 tgcttgcacg tagatcacat aagcaccaag cgcgttggcc tcatgcttga ggagattgat
     2461 gagcgcggtg gcaatgccct gcctccggtg ctcgccggag actgcgagat catagatata
     2521 gatctcacta cgcggctgct caaacttggg cagaacgtaa gccgcgagag cgccaacaac
     2581 cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta ctacggagca agttcccgag
     2641 gtaatcggag tccggctgat gttgggagta ggtggctacg tctccgaact cacgaccgaa
     2701 aagatcaaga gcagcccgca tggatttgac ttggtcaggg ccgagcctac atgtgcgaat
     2761 gatgcccata cttgagccac ctaactttgt tttagggcga ctgccctgct gcgtaacatc
     2821 gttgctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac
     2881 gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca taacaagcca
     2941 tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc
     3001 gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat gtcaactggg
     3061 ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag
     3121 tcgccataac ttcgtatagc atacattata cgaagttatc tgtaactata acggtcctaa
     3181 ggtagcgagt ttaaacacta gtatcgattc gcgacctact ccggaatatt aatagatcat
     3241 ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac
     3301 agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc caccatcggg
     3361 cgcggatctc ggtccgaaac cATGTCGTAC TACCATCACC ATCACCATCA Ccatcacggt
     3421 tctATGTGCa tggcaggcct tgcgcaacac gatgaagccg tggacaacaa attcaacaaa
     3481 gaacaacaaa acgcgttcta tgagatctta catttaccta acttaaacga agaacaacga
     3541 aacgccttca tccaaagttt aaaagatgac ccaagccaaa gcgctaacct tttagcagaa
     3601 gctaaaaagc taaatgatgc tcaggcgccg aaagtagaca acaaattcaa caaagaacaa
     3661 caaaacgcgt tctatgagat cttacattta cctaacttaa acgaagaaca acgaaacgcc
     3721 ttcatccaaa gtttaaaaga tgacccaagc caaagcgcta accttttagc agaagctaaa
     3781 aagctaaatg atgctcaggc gccgaaagta gacgcaaatt ccgcggggaa gtcaaccgat
     3841 tatgatattc caactactgc tagcgagaac ctatatttcc agggaGATTA TGATATCCCT
     3901 ACGACTGAGA ATCTTTATTT TCAGGGCatg GACTCATTGG AGTTTATCGC ATCAAAGCTC
     3961 GCAatgtctt tggttccagc aacaaattat atatatacac ccctgaatca acttaagggt
     4021 ggtacaattg tcaatgtcta tggtgttgtg aagttcttta agcccccata tctaagcaaa
     4081 ggaactgatt attgctcagt tgtaactatt gtggaccaga caaatgtaaa actaacttgc
     4141 ctgctcttta gtggaaacta tgaagccctt ccaataattt ataaaaatgg agatattgtt
     4201 cgctttcaca ggctgaagat tcaagtatat aaaaaggaga ctcagggtat caccagctct
     4261 ggctttgcat ctttgacgtt tgagggaact ttgggagccc ctatcatacc tcgcacttca
     4321 agcaagtatt ttaacttcac tactgaggac cacaaaatgg tagaagcctt acgtgtttgg
     4381 gcatctactc atatgtcacc gtcttggaca ttactaaaat tgtgtgatgt tcagccaatg
     4441 cagtattttg acctgacttg tcagctcttg ggcaaagcag aagtggacgg agcatcattt
     4501 cttctaaagg tatgggatgg caccaggaca ccatttccat cttggagagt cttaatacaa
     4561 gaccttgttc ttgaaggtga tttaagtcac atccatcggc tacaaaatct gacaatagac
     4621 attttagtct acgataacca tgttcatgtg gcaagatctc tgaaggttgg aagctttctt
     4681 agaatctata gccttcatac caaacttcaa tcaatgaatt cagagaatca gacaatgtta
     4741 agtttagagt ttcatcttca tggaggtacc agttacggtc ggggaatcag ggtcttgcca
     4801 gaaagtaact ctgatgtgga tcaactgaaa aaggatttag aatctgcaaa tttgacagcc
     4861 aatcagcatt cagatgttat ctgtcaatca gaacctgacg acagctttcc aagctctgga
     4921 tcagtatcat tatacgaggt agaaagatgt caacagctat ctgctacaat acttacagat
     4981 catcagtatt tggagaggac accactatgt gccattttga aacaaaaagc tcctcaacaa
     5041 taccgcatcc gagcaaaatt gaggtcatat aagcccagaa gactatttca gtctgttaaa
     5101 cttcattgcc ctaaatgtca tttgctgcaa gaagttccac atgagggcga tttggatata
     5161 atttttcagg atggtgcaac taaaacccca gatgtcaagc tacaaaatac atcattatat
     5221 gattcaaaaa tctggaccac taaaaatcaa aaaggacgaa aagtagcagt tcattttgtg
     5281 aaaaataatg gtattctccc gctttcaaat gaatgtctac ttttgataga aggaggtaca
     5341 ctcagtgaaa tttgcaaact ctcgaacaag tttaatagtg taattcctgt gagatctggc
     5401 cacgaagacc tggaactttt ggacctttca gcaccatttc ttatacaagg aacaatacat
     5461 cactatggat gtaaacagtg ttctagtttg agatccatac aaaatctaaa ttccctggtt
     5521 gataaaacat cgtggattcc ttcttctgtg gcagaagcac tgggtattgt acccctccaa
     5581 tatgtgtttg ttatgacctt tacacttgat gatggaacag gagtactaga agcctatctc
     5641 atggattctg acaaattctt ccagattcca gcatcagaag ttctgatgga tgatgacctt
     5701 cagaaaagtg tggatatgat catggatatg ttttgtcctc caggaataaa aattgatgca
     5761 tatccgtggt tggaatgctt catcaagtca tacaatgtca caaatggaac agataatcaa
     5821 atttgctatc agatttttga caccacagtt gcagaagatg taatctaagc ttgtcgagaa
     5881 gtactagagg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     5941 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     6001 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6061 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6121 tgtctggatc tgatcactgc ttgagcctag aagatccggc tgctaacaaa gcccgaaagg
     6181 aagctgagtt ggctgctgcc accgctgagc aataactatc ataaccccta gggtataccc
     6241 atctaattgg aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt
     6301 cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa
     6361 tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg cccacaaccg
     6421 gtggaggaaa ttctccttga agtttccctg gtgttcaaag taaaggagtt tgcaccagac
     6481 gcacctctgt tcactggtcc ggcgtattaa aacacgatac attgttatta gtacatttat
     6541 taagcgctag attctgtgcg ttgttgattt acagacaatt gttgtacgta ttttaataat
     6601 tcattaaatt tataatcttt agggtggtat gttagagcga aaatcaaatg attttcagcg
     6661 tctttatatc tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag gtttcgatta
     6721 gtttcaaaca agggttgttt ttccgaaccg atggctggac tatctaatgg attttcgctc
     6781 aacgccacaa aacttgccaa atcttgtagc agcaatctag ctttgtcgat attcgtttgt
     6841 gttttgtttt gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt tgtatttctt
     6901 tcatcactgt cgttagtgta caattgactc gacgtaaaca cgttaaatag agcttggaca
     6961 tatttaacat cgggcgtgtt agctttatta ggccgatta
//