LOCUS       pJUV_05                 6402 bp ds-DNA     circular     05-JAN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             complement(968..1939)
                     /label="repB CDS (replication protein)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     gene            complement(968..1939)
                     /label="repB gene"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     regulatory      complement(1964..1993)
                     /label="PlacUV5"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     regulatory      2468..2572
                     /label="T1"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          2471..2488
                     /label="PS1"
                     /note="sequence: agggcggcggatttgtcc"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     2582..2605
                     /label="R24"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          2629..2669
                     /label="Ptrc.x.tetO2_F"
                     /note="sequence: AAAGAATTCGCGGCCGCTTCTAGAGAGCTGTTGACAATTAATCA"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2630..2649
                     /label="prefix"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2650..2718
                     /label="Ptrc.x.tetO2"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2719..2726
                     /label="scar"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2727..2741
                     /label="BBa_B0030"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2742..2742
                     /label="T"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2743..2743
                     /label="suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2748..3776
                     /label="D-HicDH"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             2748..3776
                     /label="Translation 2748-3776"
     misc_feature    2769..2777
                     /label="linker"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(3758..3803)
                     /label="D-HicDH RV suffix"
                     /note="sequence: GTTTCTTCCTGCAGCGGCCGCTACTAGTATCATTATTTTGCTGGGCCGGTC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3777..3782
                     /label="XhoI"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             3777..3799
                     /label="Translation 3777-3799"
     CDS             3783..3799
                     /label="6xHis"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     complement(3821..3844)
                     /label="F24"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     regulatory      3851..3953
                     /label="T0"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(3909..3925)
                     /label="PS2"
                     /note="sequence: gcggcaaccgagcgttc"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             4081..4896
                     /label="neo CDS (neomycin phosphotransferase)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     gene            4081..4896
                     /label="neo gene"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4928..5173
                     /label="oriT"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     gene            complement(5182..6132)
                     /label="repC gene"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(5182..6132)
                     /label="repC CDS (replication protein)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(join(6020..6402,1..457))
                     /label="repA CDS (replication protein)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     gene            complement(join(6020..6402,1..457))
                     /label="repA gene"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 tggcgttttc ttcctcgatg tggaaccggc gcagcgtgtc cagcaccatc aggcggcggc
       61 cctcggcggc gcgcttgagg ccgtcgaacc actccggggc catgatgttg ggcaggctgc
      121 cgatcagcgg ctggatcagc aggccgtcag ccacggcttg ccgttcctcg gcgctgaggt
      181 gcgccccaag ggcgtgcagg cggtgatgaa tggcggtggg cgggtcttcg gcgggcaggt
      241 agatcaccgg gccggtgggc agttcgccca cctccagcag atccggcccg cctgcaatct
      301 gtgcggccag ttgcagggcc agcatggatt taccggcacc accgggcgac accagcgccc
      361 cgaccgtacc ggccaccatg ttgggcaaaa cgtagtccag cggtggcggc gctgctgcga
      421 acgcctccag aatattgata ggcttatggg tagccattga ttgcctcctt tgcaggcagt
      481 tggtggttag gcgctggcgg ggtcactacc cccgccctgc gccgctctga gttcttccag
      541 gcactcgcgc agcgcctcgt attcgtcgtc ggtcagccag aacttgcgct gacgcatccc
      601 tttggccttc atgcgctcgg catatcgcgc ttggcgtaca gcgtcagggc tggccagcag
      661 gtcgccggtc tgcttgtcct tttggtcttt catatcagtc accgagaaac ttgccggggc
      721 cgaaaggctt gtcttcgcgg aacaaggaca aggtgcagcc gtcaaggtta aggctggcca
      781 tatcagcgac tgaaaagcgg ccagcctcgg ccttgtttga cgtataacca aagccaccgg
      841 gcaaccaata gcccttgtca cttttgatca ggtagaccga ccctgaagcg cttttttcgt
      901 attccataaa acccccttct gtgcgtgagt actcatagta taacaggcgt gagtaccaac
      961 gcaagcacta catgctgaaa tctggcccgc ccctgtccat gcctcgctgg cggggtgccg
     1021 gtgcccgtgc cagctcggcc cgcgcaagct ggacgctggg cagacccatg accttgctga
     1081 cggtgcgctc gatgtaatcc gcttcgtggc cgggcttgcg ctctgccagc gctgggctgg
     1141 cctcggccat ggccttgccg atttcctcgg cactgcggcc ccggctggcc agcttctgcg
     1201 cggcgataaa gtcgcacttg ctgaggtcat caccgaagcg cttgaccagc ccggccatct
     1261 cgctgcggta ctcgtccagc gccgtgcgcc ggtggcggct aagctgccgc tcgggcagtt
     1321 cgaggctggc cagcctgcgg gccttctcct gctgccgctg ggcctgctcg atctgctggc
     1381 cagcctgctg caccagcgcc gggccagcgg tggcggtctt gcccttggat tcacgcagca
     1441 gcacccacgg ctgataaccg gcgcgggtgg tgtgcttgtc cttgcggttg gtgaagcccg
     1501 ccaagcggcc atagtggcgg ctgtcggcgc tggccgggtc ggcgtcgtac tcgctggcca
     1561 gcgtccgggc aatctgcccc cgaagttcac cgcctgcggc gtcggccacc ttgacccatg
     1621 cctgatagtt cttcgggctg gtttccacta ccagggcagg ctcccggccc tcggctttca
     1681 tgtcatccag gtcaaactcg ctgaggtcgt ccaccagcac cagaccatgc cgctcctgct
     1741 cggcgggcct gatatacacg tcattgccct gggcattcat ccgcttgagc catggcgtgt
     1801 tctggagcac ttcggcggct gaccattccc ggttcatcat ctggccggtg gtggcgtccc
     1861 tgacgccgat atcgaagcgc tcacagccca tggccttgag ctgtcggcct atggcctgca
     1921 aagtcctgtc gttcttcatc gggccaccaa gcgattccca cacattatac gagccggaag
     1981 cataaagtgt aaagcctaga tccgaaggat gagccgggct gaatgatcga ccgagacagg
     2041 ccctgcgggg ctgcacacgc gcccccaccc ttcgggtagg gggaaaggcc gctaaagcgg
     2101 ctaaaagcgc tccagcgtat ttctgcgggg tttggtgtgg ggtttagcgg gctttgcccg
     2161 cctttccccc tgccgcgcag cggtggggcg gtgtgtagcc tagcgcagcg aatagaccag
     2221 ctatccggcc tctggccggg catattgggc aagggcagca gcgccccaca agggcgctga
     2281 taaccgcgcc tagtggatta ttcttagata atcatggatg gatttttcca acaccccgcc
     2341 agcccccgcc cctgctgggt ttgcaggttt gggggcgtga cagttattgc aggggttcgt
     2401 gacagttatt gcaggggggc gtgacagtta ttgcaggggt tcgtgacagt tagggcgcgc
     2461 ccagctgtct agggcggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag
     2521 ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctttaatta
     2581 aagcggataa caatttcaca caggaggccg cctaggccgc ggccgcgcgA ATTCGCGGCC
     2641 GCTTCTAGAG AGCTGTTGAC AATTAATCAT CCGGCTCGTA TAATGTGTGG ATCCCCGTCA
     2701 GTGACGGAGA TTCACACATA CTAGAGATTA AAGAGGAGAA ATACTAGATG CATCATCACC
     2761 ACCACCATGG TTCCGGTAAG ATTATTGCGT ATGGAGCGCG GGTTGATGAG ATCCAATACT
     2821 TTAAGCAGTG GGCTAAAGAC ACCGGGAATA CCTTGGAGTA TCACACCGAA TTTCTTGATG
     2881 AAAATACCGT TGAATGGGCC AAGGGGTTCG ACGGTATTAA TAGTTTGCAA ACAACCCCCT
     2941 ACGCGGCCGG CGTGTTTGAA AAAATGCACG CGTATGGGAT CAAATTCTTA ACCATTAGGA
     3001 ATGTGGGCAC CGACAATATC GACATGACCG CCATGAAACA GTATGGAATC CGCTTAAGTA
     3061 ACGTCCCCGC CTATAGTCCC GCCGCCATTG CCGAATTTGC ATTGACAGAC ACCTTGTATC
     3121 TGCTACGGAA TATGGGGAAA GTGCAAGCCC AATTACAAGC GGGGGACTAT GAGAAAGCCG
     3181 GCACCTTTAT CGGAAAAGAA CTAGGTCAAC AGACCGTCGG CGTGATGGGT ACCGGTCACA
     3241 TCGGCCAGGT GGCGATCAAA TTGTTTAAAG GGTTTGGTGC TAAGGTTATA GCTTACGATC
     3301 CGTATCCCAT GAAAGGCGAC CATCCCGATT TTGATTATGT GTCCCTTGAA GACCTGTTTA
     3361 AACAAAGCGA TGTCATCGAC TTACACGTGC CTGGAATCGA ACAGAACACG CATATCATCA
     3421 ATGAGGCCGC CTTTAATCTG ATGAAACCTG GTGCGATTGT GATTAACACC GCACGGCCTA
     3481 ACTTAATTGA TACGCAGGCC ATGCTCAGCA ACTTGAAATC AGGCAAACTC GCAGGGGTGG
     3541 GTATAGACAC TTACGAATAT GAAACTGAAG ATCTTTTGAA CCTGGCGAAG CACGGTTCCT
     3601 TTAAAGATCC TTTGTGGGAT GAATTATTAG GGATGCCGAA TGTGGTATTG TCTCCTCATA
     3661 TCGCTTACTA CACCGAAACT GCTGTGCATA ATATGGTGTA CTTTAGCCTT CAACATCTGG
     3721 TGGATTTTCT GACTAAAGGC GAGACTTCTA CAGAAGTGAC CGGCCCAGCA AAATAATGAT
     3781 ACTAGTAGCG GCCGCTGCAg gcatgcaagc ttgcggccgc gtcgtgactg ggaaaaccct
     3841 ggcgactagt cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga
     3901 tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc caggggtccc
     3961 caataattac gatttaaatt tgtgtctcaa aatctctgat gttacattgc acaagataaa
     4021 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     4081 atgagccata ttcagcgtga aacgagctgt agccgtccgc gtctgaacag caacatggat
     4141 gcggatctgt atggctataa atgggcgcgt gataacgtgg gtcagagcgg cgcgaccatt
     4201 tatcgtctgt atggcaaacc ggatgcgccg gaactgtttc tgaaacatgg caaaggcagc
     4261 gtggcgaacg atgtgaccga tgaaatggtg cgtctgaact ggctgaccga atttatgccg
     4321 ctgccgacca ttaaacattt tattcgcacc ccggatgatg cgtggctgct gaccaccgcg
     4381 attccgggca aaaccgcgtt tcaggtgctg gaagaatatc cggatagcgg cgaaaacatt
     4441 gtggatgcgc tggccgtgtt tctgcgtcgt ctgcatagca ttccggtgtg caactgcccg
     4501 tttaacagcg atcgtgtgtt tcgtctggcc caggcgcaga gccgtatgaa caacggcctg
     4561 gtggatgcga gcgattttga tgatgaacgt aacggctggc cggtggaaca ggtgtggaaa
     4621 gaaatgcata aactgctgcc gtttagcccg gatagcgtgg tgacccacgg cgattttagc
     4681 ctggataacc tgattttcga tgaaggcaaa ctgattggct gcattgatgt gggccgtgtg
     4741 ggcattgcgg atcgttatca ggatctggcc attctgtgga actgcctggg cgaatttagc
     4801 ccgagcctgc aaaaacgtct gtttcagaaa tatggcattg ataatccgga tatgaacaaa
     4861 ctgcaatttc atctgatgct ggatgaattt ttctaataat taattggacc gcggtccgcg
     4921 cgttgtcctt ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat
     4981 atccatcctt tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt
     5041 ggtgtatcca acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt
     5101 gttccttctt cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc
     5161 gaggctggcc gtaggccggc ctcagcctgc cgccttgggc cgggtgatgt cgtacttgcc
     5221 cgccgcgaac tcggttaccg tccagcccag cgcgaccagc tccggcaacg cctcgcgcac
     5281 ccgctggcgg cgcttgcgca tggtcgaacc actggcctct gacggccaga catagccgca
     5341 caaggtatct atggaagcct tgccggtttt gccggggtcg atccagccac acagccgctg
     5401 gtgcagcagg cgggcggttt cgctgtccag cgcccgcacc tcgtccatgc tgatgcgcac
     5461 atgctggccg ccacccatga cggcctgcgc gatcaagggg ttcagggcca cgtacaggcg
     5521 cccgtccgcc tcgtcgctgg cgtactccga cagcagccga aacccctgcc gcttgcggcc
     5581 attctgggcg atgatggata ccttccaaag gcgctcgatg cagtcctgta tgtgcttgag
     5641 cgccccacca ctatcgacct ctgccccgat ttcctttgcc agcgcccgat agctaccttt
     5701 gaccacatgg cattcagcgg tgacggcctc ccacttgggt tccaggaaca gccggagctg
     5761 ccgtccgcct tcggtcttgg gttccgggcc aagcactagg ccattaggcc cagccatggc
     5821 caccagccct tgcaggatgc gcagatcatc agcgcccagc ggctccgggc cgctgaactc
     5881 gatccgcttg ccgtcgccgt agtcatacgt cacgtccagc ttgctgcgct tgcgctcgcc
     5941 ccgcttgagg gcacggaaca ggccgggggc cagacagtgc gccgggtcgt gccggacgtg
     6001 gctgaggctg tgcttgttct taggcttcac cacggggcac ccccttgctc ttgcgctgcc
     6061 tctccagcac ggcgggcttg agcaccccgc cgtcatgccg cctgaaccac cgatcagcga
     6121 acggtgcgcc atagttggcc ttgctcacac cgaagcggac gaagaaccgg cgctggtcgt
     6181 cgtccacacc ccattcctcg gcctcggcgc tggtcatgct cgacaggtag gactgccagc
     6241 ggatgttatc gaccagtacc gagctgcccc ggctggcctg ctgctggtcg cctgcgccca
     6301 tcatggccgc gcccttgctg gcatggtgca ggaacacgat agagcacccg gtatcggcgg
     6361 cgatggcctc catgcgaccg atgacctggg ccatggggcc gc
//