LOCUS       pegRNA-HEK3_del1-5-NGG  2322 bp ds-DNA     circular     20-APR-2022
DEFINITION  .
KEYWORDS    "accession:addgene_132777_255290"                                  
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     primer_bind     430..449
                     /label="Amp-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        complement(668..772)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     source          join(798..2322,1..797)
                     /label="source"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     879..899
                     /label="hU6-F"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        879..1119
                     /label="U6 promoter"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     1050..1069
                     /label="LKO.1 5'"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1129..1148
                     /label="HEK3-spacer"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1149..1224
                     /label="gRNA scaffold"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1225..1266
                     /label="HEK3_5bp-del-extension"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(1370..1958)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(1450..1469)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(join(2129..2322,1..667))
                     /label="AmpR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
       61 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
      121 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
      181 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
      241 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
      301 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
      361 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
      421 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
      481 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
      541 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
      601 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
      661 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
      721 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
      781 cccgaaaagt gccacctgac gtcgctagct gtacaaaaaa gcaggcttta aaggaaccaa
      841 ttcagtcgac tggatccggt accaaggtcg ggcaggaaga gggcctattt cccatgattc
      901 cttcatattt gcatatacga tacaaggctg ttagagagat aattagaatt aatttgactg
      961 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt
     1021 ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt
     1081 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccGGG CCCAGACTGA
     1141 GCACGTGAGT TTTAGAGCTA GAAATAGCAA GTTAAAATAA GGCTAGTCCG TTATCAACTT
     1201 GAAAAAGTGG CACCGAGTCG GTGCTGGAGG AAGCAGGGCT TCCTTTCCTC TGCCGTGCTC
     1261 AGTCTGtttt tttaagcttg ggccgctcga ggtacctctc tacatatgac atgtgagcaa
     1321 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     1381 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     1441 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     1501 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     1561 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     1621 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     1681 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     1741 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     1801 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     1861 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     1921 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     1981 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     2041 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     2101 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     2161 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     2221 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gatccacgct
     2281 caccggctcc agatttatca gcaataaacc agccagccgg aa
//