LOCUS       pCMV-T7-NLS(SV40)-anCas(PCA)-BPNLS(SV40)-P2A-EGFP_(LTH1441)       10434 bp    DNA     circular UNA 19-APR-2022
DEFINITION  .
ACCESSION   urn.local...j-epvohi6
VERSION     urn.local...j-epvohi6
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     enhancer        235..614
     promoter        615..818
     promoter        863..881
     misc_feature    923..932
                     /note="Geneious type: motif"
                     /standard_name="Kozak"
     CDS             929..931
                     /standard_name="start codon"
     CDS             932..952
                     /codon_start=1
                     /locus_tag="<html><body><br></body></html>"
                     /note="<html><body><br></body></html>"
                     /product="<html><body>nuclear localization signal of SV40
                     (simian virus 40) large T antigen</body></html>"
                     /protein_id="<html><body><br></body></html>"
                     /transl_table=1
                     /translation="PKKKRKV"
                     /standard_name="SV40 NLS"
     CDS             953..958
                     /standard_name="linker"
     CDS             959..5062
                     /standard_name="anCas(PCA)"
     CDS             5063..5077
                     /standard_name="linker"
     CDS             5078..5128
                     /standard_name="BPNLS(SV40)"
     CDS             5129..5137
                     /standard_name="linker"
     CDS             5138..5203
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                     PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDKRPAATKKAG
                     QAKKKKDYKDDDDKGSGATNFSLLKQAGDVEENPGPTEYKPTVRLATRDDVPRAVRTL
                     AAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPES
                     VEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGL
                     GSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKP
                     GA*"
                     /standard_name="P2A"
     CDS             5204..5920
                     /standard_name="eGFP"
     CDS             5921..5923
                     /standard_name
     polyA_signal    6031..6255
     rep_origin      6301..6729
     promoter        6743..7072
     rep_origin      6923..7058
     CDS             7139..7933
                     /codon_start=1
                     /gene="<html><body><i>aph(3')-II</i> (or
                     <i>nptII</i>)</body></html>"
                     /note="<html><body>confers resistance to neomycin,
                     kanamycin, and G418 (Geneticin®)</body></html>"
                     /product="<html><body>aminoglycoside phosphotransferase
                     from Tn5</body></html>"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /standard_name="NeoR/KanR"
     polyA_signal    8107..8228
     primer_bind     complement(8277..8293)
                     /note="<html><body>common sequencing primer, one of
                     multiple similar variants</body></html>"
                     /standard_name="M13 rev"
     protein_bind    8301..8317
                     /bound_moiety="<html><body><i>lac</i> repressor encoded by
                     <i>lacI</i></body></html>"
                     /note="<html><body>The <i>lac</i> repressor binds to the
                     <i>lac</i> operator to inhibit transcription in <i>E.
                     coli</i>. This inhibition can be relieved by adding
                     lactose or isopropyl-β-D-thiogalactopyranoside
                     (IPTG).</body></html>"
                     /standard_name="lac operator"
     promoter        join(complement(8350..8355),complement(8332..8349),
                     complement(8325..8331))
     protein_bind    8370..8391
                     /bound_moiety="<html><body><i>E. coli</i> catabolite
                     activator protein</body></html>"
                     /note="<html><body>CAP binding activates transcription in
                     the presence of cAMP.</body></html>"
                     /standard_name="CAP binding site"
     rep_origin      complement(8679..9267)
     CDS             join(complement(10230..10298),complement(9438..10229))
                     /codon_start=1
                     /gene="<html><body><i>bla</i></body></html>"
                     /note="<html><body>confers resistance to ampicillin,
                     carbenicillin, and related antibiotics</body></html>"
                     /product="<html><body>β-lactamase</body></html>"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVG
                     YIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLV
                     EYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGP
                     LLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQI
                     AEIGASLIKHW*"
                     /standard_name="AmpR"
     promoter        complement(10299..10403)
ORIGIN      
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgccaccat gcccaagaaa aagcggaagg tgcccaagat
      961 gaagaaacca tacagcatcg gcctggacat cggcaccaac agcgtgggct gggccgtgat
     1021 cacagatgat tacaaggtgc ccgctaagaa gatgaaggtg ctgggcaaca ccgacagaca
     1081 aagtattaag aaaaacctga tcggagccct gctgtttgac agcggagaga cagctgaggc
     1141 caccagactg aaacggacag ctcgtagaag gtataccaga agaaagaaca gaatctgtta
     1201 cctgcaggag atctttagcg aagagatggc caaggtggac gacagcttct ttcaccgcct
     1261 ggaagaatct ttcctggtgc ccgaggataa gaagtacgat agacacccta tcttcggcaa
     1321 tctggcggat gaagtggcct accacgaaaa ctaccccacc atctaccacc tgagaaagaa
     1381 gctggccgac tctacagaga aagccgacct gagactgatc tacctggccc ttgcccatat
     1441 catcaagttc cggggccact tcctgatcga gggcgacctt aacgccgaaa acaccgacgt
     1501 gcagaaactg ttccaccagc tggtggatac ctacaaccag ctgttcgagg aggaccagct
     1561 ggatactgag accatcgacg caaaggccat cctgaccgct aagattagca agagcagacg
     1621 cctggaaaac ctgatcagcc aaatccctgg acagaaaaaa aacggcctgt ttggcaacct
     1681 gatcgccctg agcctgggcc tgacccctaa tttcaagtct aacttcgacc tgtccgagga
     1741 tgccaaactg cagctgtcca aggacaccta cgaggaggat ctggacaatc tgcttgctca
     1801 gatcggcgat cagtacgccg acctatttct cgccgccaag aacctgagcg atgccattct
     1861 gctctccgac atcttgacag tcaatgacga gagcacaaaa gcccctctgt ctgcttccat
     1921 gatcaagcgg tacgaagaac accagcagga cctggccctg ctgaaacagc tggtgaagga
     1981 acagctgccc gagaaataca aagagatctt ctctgacaaa tctaagaacg gatacgccgg
     2041 atatatcgat ggcaagacct cccaggagga gttctacaag tacatcaaac ctatcctgtc
     2101 caagctcgat ggcgccgagg aattccttgc caagatcgat agagaagatt ttctgcgcaa
     2161 gcagagaacc tttgacaacg gctccatccc ccaccagatc cacctcgaag agctgcatgc
     2221 gatcctgaga agacaggagg aatactaccc gttcctgaag gacaatcaag agaagatcga
     2281 aaaaatattg accttccgaa tcccatacta cgtgggacct ctggccagag gaaatagcag
     2341 attcgcctgg ctgaccagaa aatctgatga ggccatcaca ccctggaact ttgaggaggt
     2401 cgtggataaa gaagcctccg cccaggcgtt catcgagcgt atgacaaact tcgatacgta
     2461 cctgcccaac gaaaaggtgc ttccaaaaca ttctctgctg tacgagacct tcacagtgta
     2521 caacgagttg acaaaagtga agtacgtgac cgagggcatg accaagcctc aatttctcag
     2581 cgcggagcag aagcaagcca tcgtggacct gctgttcaag aagaaccgga aggtgacagt
     2641 gaaacagctt aaggaagact atttcaagaa gatcgagtgc ttcgactccg tggacatcac
     2701 aggagttgaa gacaggttca acgccagcct gggcacatac cacgatctgc tgaagatcat
     2761 caaggacaag gacttcctgg acaacgagga gaatgaagat atcctggaag atatcgtact
     2821 gacactgaca ctgttcgagg atagagagat gatcgaaaag agactggcca agtacgcgga
     2881 cctgttcgac aagaaggtgc tgaagaaact gaagagacgg cactacaccg gatggggcag
     2941 actgtcccgg aaactgatca atggaatcag agataagcag agcggcaaga ccatcctgga
     3001 ttttctgaag gccgatggat tcgccaatag aaactttatg cagctgatta acgacgattc
     3061 cctgagcttt aaggaggaaa tcgagaaggc ccaagtgatc ggccagaccg actccctgca
     3121 cgaggtggtg gccgacttag ctggcagccc cgccatcaag aagggcatcc tgcagacaat
     3181 caagatcgtg gacgagctgg tgaaggttat gggccggcac aaccccgaaa atatcgtcat
     3241 cgaaatggcc agagagaacc agaccaccgc ccaaggcatc aagaatagta gacagcggat
     3301 gaagcggctg gaggaggtgc tgaagaaact gggcagcaac atcctgaagg agcaccccgt
     3361 ggacaacacc cagctgcaga acgacaggct gtacctgtac tacctgcaaa atggcaagga
     3421 catgtacacc ggccaggagc tggacattga caacctcagc cagtacgaca ttgaccacat
     3481 catccctcag agtttcatca aggacaacag catcgacaac aaggtcctca ccagcagcga
     3541 ggaaaacaga ggcaagagcg acaatgtgcc tagcatcgag gtggtgcgga agatgaagag
     3601 ctactggcag aaactgctga acgccggcct gatttctcag cgaaagttcg ataatctgac
     3661 caaggcagag cggggtggac tgactgagag cgacaaagct ggcttcatca agcggcagct
     3721 ggtcgagaca agacagatca ccaagcacgt ggcccaaatc ctggacagca gattcaacac
     3781 tgagcgggac gaaaacgaca aacctatcag aaacgtgaag atcatcaccc tgaagtctaa
     3841 gctggtgtcg gacttcagaa aagacttcgg cctgtacaag gttagagaga ttaacgatta
     3901 ccaccacgcc cacgacgcct acctgaacgc cgtggtcgga acagccctgc tgaagaagta
     3961 ccctaagctg gagcctgagt tcgtgtatgg cgactacaaa aaatatgatc tgagaaagct
     4021 gatcgccaag agcgacaagg agagaggcaa ggccacagca aagatgttct tctacagcaa
     4081 catcatgaac ttcttcaaga cagaagtgaa gctggctaat ggcacaatca tcaagagacc
     4141 tgtgatcgag accaacgagg aaaccggcga gatcgtgtgg gacaaggaaa aggatttcgc
     4201 caccgtgcgg aaggtgctga gctaccccca ggtgaacatc gtcaagaaga ccgaggtgca
     4261 gaccggcggc ttcagcaagg aaagcatcct ccctaagggc aacagcgaca agctaatccc
     4321 aagaaagaac aactgggacc ctaagaagta cggcggattc gactctccta ccgtggccta
     4381 tagcgtgctg gttgtggcca aggtggaaaa gggcaaggcc aagaagctga agaccgtgaa
     4441 agaactggtg ggcatcacca tcatggaaag aagcgccttc gaaaagaatc ctattgcctt
     4501 tctcgaagct aagggctacc aggacatcca ggaggacctg ataatcaaac tgcctaaata
     4561 cagcctgttc gagctggaga acggccggag acggctcctg gctagcgcta aagagctaca
     4621 gaagggcaac gagatggtgc tgcctgccca cctggtgacc ttcctgtacc atgccagccg
     4681 gatcgataaa agcacgggct ctagcgaaaa cttagagcaa aagcaggagt acgtggaaca
     4741 gcacaagcac gagttcgatg agatcctgga ctacatcatt gacttttccg agagatacat
     4801 cctggccgat aagaacctgg agaagatcaa aagcctgtat aaccagaacg acgactccga
     4861 catcaacgag ttggcctctt ctttcatcaa tctgttcacc ttcaccgccc tgggcgcccc
     4921 tgctgccttt aagttcttcg acgctacaat cgatagaaag cggtatacca gcaccaagga
     4981 ggtgctgaat gctacactga tccaccagag catcaccgga ctgtacgaga ctagaatcga
     5041 cctttcccag ttaggcggcg atcactctgg cggctcaaaa agaaccgccg acggcagcga
     5101 attcgagccc aagaagaaga ggaaagtcgg aggcggtgga agcggcgcaa caaacttctc
     5161 tctgctgaaa caagccggag atgtcgaaga gaatcctgga ccgatggtga gcaagggcga
     5221 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     5281 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     5341 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     5401 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     5461 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     5521 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     5581 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     5641 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     5701 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     5761 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     5821 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     5881 cgccgccggg atcactctcg gcatggacga gctgtacaag tgacatcatc atcaccacgc
     5941 ctctggaaca ggcggaccta aaaagaagag gaaggtttaa gcggccgctc gagtctagag
     6001 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     6061 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     6121 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     6181 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     6241 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     6301 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6361 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6421 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6481 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6541 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6601 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6661 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6721 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     6781 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     6841 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     6901 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6961 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     7021 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     7081 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
     7141 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     7201 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     7261 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     7321 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     7381 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     7441 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     7501 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     7561 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     7621 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     7681 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     7741 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     7801 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     7861 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     7921 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     7981 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     8041 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     8101 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8161 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8221 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     8281 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8341 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8401 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8461 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8521 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8581 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8641 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8701 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8761 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8821 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8881 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8941 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9001 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9061 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     9121 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9181 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9241 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9301 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9361 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9421 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9481 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9541 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9601 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9661 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9721 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9781 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9841 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9901 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9961 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10021 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10081 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10141 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10201 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10261 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10321 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10381 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
