LOCUS       pCMV-T7-NLS(SV40)-anCas(BCA)-BPNLS(SV40)-P2A-EGFP_(LTH1384)       10389 bp    DNA     circular UNA 19-APR-2022
DEFINITION  .
ACCESSION   urn.local...c-epvohi3
VERSION     urn.local...c-epvohi3
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     enhancer        235..614
     promoter        615..818
     promoter        863..881
     misc_feature    901..910
                     /note="Geneious type: motif"
                     /standard_name="Kozak"
     CDS             907..909
                     /standard_name="start codon"
     CDS             910..930
                     /codon_start=1
                     /locus_tag="<html><body><br></body></html>"
                     /note="<html><body><br></body></html>"
                     /product="<html><body>nuclear localization signal of SV40
                     (simian virus 40) large T antigen</body></html>"
                     /protein_id="<html><body><br></body></html>"
                     /transl_table=1
                     /translation="PKKKRKV"
                     /standard_name="SV40 NLS"
     CDS             931..936
                     /standard_name="linker"
     CDS             937..4956
                     /standard_name="anCas(BCA)"
     CDS             4957..4971
                     /standard_name="linker"
     CDS             4972..5022
                     /standard_name="BPNLS(SV40)"
     CDS             5023..5031
                     /standard_name="linker"
     CDS             5032..5097
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                     PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDKRPAATKKAG
                     QAKKKKDYKDDDDKGSGATNFSLLKQAGDVEENPGPTEYKPTVRLATRDDVPRAVRTL
                     AAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPES
                     VEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGL
                     GSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKP
                     GA*"
                     /standard_name="P2A"
     CDS             5098..5814
                     /standard_name="eGFP"
     CDS             5815..5817
                     /standard_name
     polyA_signal    5986..6210
     rep_origin      6256..6684
     promoter        6698..7027
     rep_origin      6878..7013
     CDS             7094..7888
                     /codon_start=1
                     /gene="<html><body><i>aph(3')-II </i>(or<i>
                     nptII)</i></body></html>"
                     /note="<html><body>confers resistance to neomycin,
                     kanamycin, and G418 (Geneticin®)</body></html>"
                     /product="<html><body>aminoglycoside phosphotransferase
                     from Tn5</body></html>"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /standard_name="NeoR/KanR"
     polyA_signal    8062..8183
     primer_bind     complement(8232..8248)
                     /note="<html><body>common sequencing primer, one of
                     multiple similar variants</body></html>"
                     /standard_name="M13 rev"
     protein_bind    8256..8272
                     /bound_moiety="<html><body><i>lac</i> repressor encoded by
                     <i>lacI</i></body></html>"
                     /note="<html><body>The <i>lac</i> repressor binds to the
                     <i>lac</i> operator to inhibit transcription in <i>E.
                     coli</i>. This inhibition can be relieved by adding
                     lactose or isopropyl-β-D-thiogalactopyranoside
                     (IPTG).</body></html>"
                     /standard_name="lac operator"
     promoter        join(complement(8305..8310),complement(8287..8304),
                     complement(8280..8286))
     protein_bind    8325..8346
                     /bound_moiety="<html><body><i>E. coli</i> catabolite
                     activator protein</body></html>"
                     /note="<html><body>CAP binding activates transcription in
                     the presence of cAMP.</body></html>"
                     /standard_name="CAP binding site"
     rep_origin      complement(8634..9222)
     CDS             join(complement(10185..10253),complement(9393..10184))
                     /codon_start=1
                     /gene="<html><body><i>bla</i></body></html>"
                     /note="<html><body>confers resistance to ampicillin,
                     carbenicillin, and related antibiotics</body></html>"
                     /product="<html><body>β-lactamase</body></html>"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVG
                     YIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLV
                     EYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGP
                     LLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQI
                     AEIGASLIKHW*"
                     /standard_name="AmpR"
     promoter        complement(10254..10358)
ORIGIN      
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccaccatgc ccaagaaaaa gcggaaggtg cccaagatga agaaggacta ttctatcgga
      961 ctggacatcg gcacaaacag cgtgggctgg gccgtgatca cagacgacta caaggtcgtg
     1021 cgcaagaaaa tgaaggtgct gggcaacacc gataaaaaga gcatcaagaa aaacctgtgg
     1081 ggagtgagac tgttcgactc tggcgaaacc gccgaggata caagactgaa gcgaaccacc
     1141 agacggagat acaccagaag aagaaaccgc atctgctacc tgcaggaaat cttccaggaa
     1201 gagatgaaca aggtcgacga cagcttcttc caccgcctgg atgaaagctt cctggtgccc
     1261 gaggataaaa agtacgacag acaccctatc tttggcaacc tggaagaaga agtggcctac
     1321 catgagcaat accctaccat ctaccacctg cggaagcacc tcgccgattc tagtgagaag
     1381 gccgatctga gactggttta cctggccctg gcccatatca tcaaatacag aggccacttc
     1441 ctgatcgaag gcgacctgaa caccgagaac actgacgtgg aagagctgtt caaacagttc
     1501 gtgcaggtgt acaaccagac cttcgaggaa caacacctgt cggatgagac catcgacgtg
     1561 gaggaaatcc tgacagaaaa agtgtccaag agcagacggg ccgagaacgt gctgaagcta
     1621 tttccaaatg agaagaaaaa cggcctgttt ggccagctga tcaagctgat cgttggcctg
     1681 cagggcaact tcaagaaagt gttcgacctc agcgaggacg ccaagctcca attttctaag
     1741 gatacctatg aggaggacct ggaaaacctt ctggccatga tcggggacga gtacgccgac
     1801 ctgttcctgg ccgccaaaaa cctgtacgac gctatcctgc tttctggaat cctgaccaca
     1861 accgataata acaccaaggc caagctgagc gccagcatga tcaagcggta cgaggagcac
     1921 cagaaggacc tggcacaact gaagcagttc atcaaagagc acctgccaga caagtacaat
     1981 gaaatcttca gcgactccag caaaaacggc tatgccggct acatcgaggg caaaaccagc
     2041 caggaggatt tctacaagta cctgaagaag atcctgtcta agattgatgg cgctgaatac
     2101 ttcctggaga aaattgaaca ggaaaatttt ctgaggaagc aaagaacctt tgacaacggc
     2161 gtgatcccgc accagatcca cctggaggag ctgagagcca tcctgaggcg tcagggcaag
     2221 tactacccat tcctcaaaga gaaccaggag aagatagagc agatcctgac atttagaatc
     2281 ccctactacg tgggcccact ggctagagga aatagccggt tcgcctggct gacacggaag
     2341 tctgacgagc ccatcacacc ttggaacttc gacgaagtcg tggacaagga gaagtccgcc
     2401 gaggccttca tcgagagaat gaccaactac gacacctacc tgcctaacga aaaggtactg
     2461 cccaaacaca gcctgctgta cgagaagttc accgtgttca acgaattgac aaaggttcgg
     2521 tacgtgacag accggggcaa gacccagaac ttctccgccg agcagaagca ggagatcttc
     2581 gacgacctgt tcaagaagaa ccggaaggtc acaaagaaaa agctggagaa ctatctgaag
     2641 aaagaatacg agtacttcga cagccctgat atcaccggaa ttgaggacga gttcaacgcc
     2701 agcctgggca cctatcatga tctgctgaag atcctgaagt ctaaggactt cctggacgat
     2761 gaggaaaacg aggaaatcct ggaggatatc gtgaagatcc tgaccgtgtt cgaggacaga
     2821 aagatgatca gaaagcggct ggagaaatac agcgacatcc tcaccgagga acagttgaag
     2881 aagctggagc ggcggcacta cacaggctgg ggcagattga gcagaaagct tatcaacggc
     2941 atccgcgaca aacagagcgg caagacaatt ttagattacc tgatcgacga cgacagcagt
     3001 aaccggaact tcatgcagct gatcaacgat gacaatttga gctttaagga ggaaatcgag
     3061 aaggcccagg tgatcggaga gaccgagaac ctgcacgaga tagtgcaaga cctgcctggc
     3121 agccctgcta tcaagaaggg catcctgcag tctctgaaga ttgtggacga gctcgtgaag
     3181 gtgatggggc acgaacctaa gaacatcgtg gtcgaaatgg ccagagagaa ccagacgacc
     3241 agcaggggca gacggaacag caagcagcgg ctgaagagac tggaggaggc tcttaagaac
     3301 ctgggcagca acctgctgaa agaacacccc gtggacaatc agcagctgca gaacgacaga
     3361 ctgtacctat actacctgca aaatggcaag gatatgtaca caggacagga gctggacatc
     3421 gataatctgt ccaactacga catcgatcac atcatccccc agagtttcat caaggataat
     3481 agcatcgata accgagtgct ggtgagcagc gaggaaaata gaggcaagtc tgataatgtg
     3541 ccttctaagg aagttgtgag aaagatgaaa tctttttggc agaaactgct gaatgctggc
     3601 ctgatcagcc agagaaaatt cgacaacctg acaaaggctg agagaggcgg cctgaccgaa
     3661 gacgacaaag ctggattcat caagagacag ctggtggaaa caagacagat caccaagcac
     3721 gtggccaaca tcctagacag ccggttcaac acagagaagg acgaggagga caacaccatc
     3781 agaaacgtga agattatcac cctgaagtcc aacctggtgt cccagttcag aaaggatttc
     3841 ggcctgtaca aggttcggga aattaacgac taccaccacg cccacgacgc ctacctgaat
     3901 gccgtggtgg gcaccgccct gctgaagaag tatcctcaac tggagcctga gttcgtgtac
     3961 ggcgactacc ggaagaacaa cgccagagag gaaaacaagg ccacagccaa gaagcacttc
     4021 tacagcaata tcatgaactt cttcgcctct gaagtgaaga ttgccgacga aaccggcgag
     4081 atcgtgtggg acaaagaaaa ggacatagct accgtgcgga aggtgctgtc ctaccaccag
     4141 gtgaacatcg tgaagaaagt ggaggtgcag aagggaggat tctccaagga aaccatcctc
     4201 cccaagggca acagcaacaa gctcatcccc agaaagaaca actgggaccc taagaagtac
     4261 ggcggcttcg acagccccac cgtggcttat agcgtgctgg tgacctacga tatcgagaag
     4321 ggaaagaaaa agaagctgaa gaccgtgaaa gagctggtcg gcatcaccat catggagcgg
     4381 agcgcctttg aaaagaaccc catcgcctac ctggaaaaaa aaggctacca gaatcctcag
     4441 gaggacgtgc tgatcaaact gcctaagtac tccctgttcg agctggaaaa cggcagacgt
     4501 cggatgctgg ccagcgctaa ggaactccaa aagggcaatc agatggtgct tcctgcccat
     4561 ctggtgaccc tgctgtacca cgccaaacgg atcgataaga gcaacaattc tgagagcctg
     4621 gaatacgtgg aagaacacag aaatgaattc gacgagctgc tggactatat cattgatttc
     4681 agcgagaagt atatcctggc tgataagaac ctggaaaaga tcaagaagct ttacgagcag
     4741 aacaacgagg ccgacatcaa ggagctggcc aagagcttta tcaacctgct gaccttcacc
     4801 gccatgggcg cccctgccgc tttcaagttc ttcggcgaaa caatcgacag aaaaagatac
     4861 acaagcatca aggaactgct caacgccacc ctcatccacc agagcatcac aggtctgtac
     4921 gagacaagaa tcgacctggg caagctggga gaggatcact ctggcggctc aaaaagaacc
     4981 gccgacggca gcgaattcga gcccaagaag aagaggaaag tcggaggcgg tggaagcggc
     5041 gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg
     5101 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     5161 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     5221 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     5281 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     5341 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     5401 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     5461 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     5521 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     5581 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     5641 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     5701 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     5761 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtgacat
     5821 catcatcacc acgcctctgg aacaggcgga cctaaaaaga agaggaaggt ttaaggtacc
     5881 gagctcggat ccactagtcc agtgtggtgg aattctgcag atatccagca cagtggcggc
     5941 cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     6001 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     6061 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     6121 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     6181 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     6241 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6301 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6361 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6421 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6481 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6541 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     6601 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     6661 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     6721 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     6781 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     6841 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     6901 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6961 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     7021 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
     7081 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     7141 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     7201 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     7261 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     7321 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     7381 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     7441 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     7501 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     7561 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     7621 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     7681 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     7741 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     7801 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     7861 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     7921 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     7981 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     8041 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     8101 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     8161 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     8221 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     8281 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     8341 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     8401 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     8461 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8521 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8581 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8641 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8701 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8761 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8821 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8881 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8941 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9001 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9061 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9121 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9181 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9241 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9301 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9361 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9421 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9481 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9541 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9601 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9661 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9721 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9781 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9841 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9901 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9961 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    10021 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    10081 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    10141 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10201 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    10261 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10321 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10381 cctgacgtc
//
