LOCUS       Exported               12026 bp ds-DNA     circular SYN 22-APR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 7, 2022 from SnapGene Viewer 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12026
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        618..821
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        2602..3891
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
                     /note="color: #ffffff; direction: RIGHT"
     regulatory      3901..3910
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     misc_feature    3907..5577
                     /label=LGI1
                     /note="color: #ffff99"
     misc_feature    5578..5613
                     /label=Linker
                     /note="color: #a6acb3"
     misc_feature    5614..6687
                     /label=pHmScarlet
                     /note="color: #ff0000"
     misc_feature    6718..7306
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(7189..7200)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 7188"
                     /translation="IEGR"
     primer_bind     complement(7309..7325)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     LTR             7831..8011
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    8043..8267
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      8313..8741
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        8755..9084
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      8935..9070
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        9132..9179
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
                     /note="color: #ffffff; direction: RIGHT"
     CDS             9198..9572
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /note="color: #ccffcc"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9702..9823
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(9872..9888)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    9896..9912
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(9920..9950)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 9920 .. 9926 / #ffffff / -10
                        2: 9927 .. 9944 / #ffffff
                        3: 9945 .. 9950 / #ffffff / -35"
     protein_bind    9965..9986
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(10274..10862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(11033..11893)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 11033 .. 11824 / #ccffcc
                        2: 11825 .. 11893 / #ccffcc / signal sequence
                     Cleavage site after base 11824"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11894..11998)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt
     2641 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag
     2701 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat
     2761 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca
     2821 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg
     2881 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga
     2941 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg
     3001 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga
     3061 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac
     3121 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg
     3181 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa
     3241 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt
     3301 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga
     3361 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc
     3421 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa
     3481 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag
     3541 tttgttcaca tccccttctc caaccccctc agtacatcac cctgggagaa caaggtccac
     3601 ttgcttctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caacggagga
     3661 gcaggttttg aagtgaaagg caggcaggtg ttggggaggc agttaccggg gcaacgggaa
     3721 cagggcgttt cggaggtggt tgccatgggg acctggatgc tgacgaaggc tcgcgaggct
     3781 gtgagcagcc acagtgccct gctcagaagc cccaagctcg tcaatcaagc tggttctcca
     3841 tttgcactca ggagcacggg caggcgagtg gcccctagtt ctgggggcag cgggggatcc
     3901 gccaccatgg aaagcgagag catcagaagg atgggcaacg cttgcatccc tctgaagagg
     3961 atcgcctact tcctgtgcct gttcagcgtg gtgctgctga ccgagggaaa gaagcctgcc
     4021 aagcctaagt gccctgccgt gtgcacctgt agcaaggata acgccctgtg cgagaacgcc
     4081 cgcagcatcc ctagaacagt gcctccagac gtgatcagcc tgagctttgt gcggagcggc
     4141 ttcaccgaga tcagcgaggg atctttcctg ttcaccccta gcctgcagct gctgctgttc
     4201 accagcaaca gcttcgacgt gatctccgac gacgccttca tcggcctgcc tcacctggaa
     4261 tacctgttca tcgagaacaa caacatcaag agcatctcca ggcacacctt caggggcctg
     4321 aagtccctga tccacctgag cctggccaac aacaacctgc agaccctgcc taaggacatc
     4381 ttcaaaggcc tggacagcct gaccaacgtg gacctgagag gcaactcctt caactgcgac
     4441 tgcaagctga agtggctggt ggaatggctg ggccacacaa acgctaccgt cgaggacatc
     4501 tactgcgagg gacctccaga gtacaagaag cggaagatca actctctgag ccccaaggac
     4561 ttcgactgca tcatcaccga gttcgccaag agccaggacc tgccttacca gagcctgagc
     4621 atcgacacct tctcttacct gaacgacgag tacgtcgtga tcgcccagcc ttttaccggc
     4681 aagtgcatct tcctggaatg ggaccacgtg gaaaagacct tccggaacta cgacaacatc
     4741 accggcacca gcaccgtcgt gtgcaagcct atcgtgatcg acacccagct gtacgtgatc
     4801 gtggctcagc tgttcggcgg cagccacatc tacaagagag atggcttcgc caacaagttc
     4861 atcaagatcc aggacatcga ggtgctgaag atcagaaagc ccaacgacat cgagacattc
     4921 aagatcgagg ataactggta cttcgtggtg gccgacagca gcaaggccgg cttcacaaca
     4981 atctacaagt ggaacggcaa cggcttctac agccaccagt ctctgcacgc ctggtacaga
     5041 gacaccgacg tcgagtacct ggaaatcgcc aggcctcctc tgacactgag aacccctcac
     5101 ctgatcctga gcagctctag ccagaggcct gtgatctacc agtggtccaa ggccacacag
     5161 ctgttcatca accagacaga catccccaac atggaagatg tgtacgccgt gaagcacttc
     5221 agcgtgaagg gcgacgtgta catctgcctg accagattca tcggcgacag caaagtgatg
     5281 aagtggggcg gcagcagctt tcaggacatc cagaggatgc ctagcagggg cagcatggtg
     5341 tttcagcccc tgcagatcaa caactaccag tacgccatcc tgggcagcga ctacagcttc
     5401 acccaggtgt acaattggga cgccgagaag gccaagttcg tgaagttcca agagctgaac
     5461 gtgcaggccc ctagatcttt cacccacgtg tcaatcaaca agcggaactt cctgttcgcc
     5521 agcagcttca agggcaacac ccagatctac aagcacgtca tcgtggatct gagcgccagc
     5581 ggctctacta gtggtggatc tggtggaacc ggcatgtcgg ctaccgctgc caccgtcccg
     5641 cctgccgccc cggccggcga gggtggcccc cctgcacctc ctccaaacct tactagtaac
     5701 aggagactgc agcagaccca ggcccaggtg gatgaggtgg tggacatcat gagggtgaat
     5761 gtggacaagg tcctggagcg ggaccagaag ttgtcggagc tggatgaccg tgcagatgcc
     5821 ctccaggcag gggcctccca gtttgaaaca agtgcagcca agctcaagcg caaatactgg
     5881 tggaaaaacc tcaagatgat gatcatcttg ggagtgatct gcgccatcat cctcatcatc
     5941 atcatcgttt acttcagcac tagcggcgga agcggcgggg atcccaccat ggtgagcaag
     6001 ggagaggccg tgatcaagga gttcatgaga ttcaaggtgc acatggaggg aagcatgaac
     6061 ggacacgagt tcgagatcga gggcgagggc gagggaaggc catacgaggg gacccagaca
     6121 gcaaagctga aggtgacaaa gggcggaccc ctgcctttta gctgggacat cctgagccca
     6181 caatttatgt atggcagcag agcctttatt aagcaccccg ccgacatccc cgactactac
     6241 aaacaatcct ttcccgaggg ctttaaatgg gaacgggtga tgaacttcga agacggcggc
     6301 gccgtgaccg tgacccagga taccagcctg gaagacggaa ccctgatcta caaagtgaag
     6361 ctcagaggca ccaacttccc ccccgacggc cccgttatgc agaagaagac aatgggttgg
     6421 gaggccagct gtgagagact ctaccccgag gacggcgtgc tcaagggcga cattttgaaa
     6481 gttctgcgcc tgaaggacgg cggaagatac ctggccgact tcaagaccac ctacaaggcc
     6541 aaaaagcccg tgcagatgcc cggcgcatac aacgtggaca gacagctgac gataaccagc
     6601 cacaacgaag actacaccgt ggtggagcaa tacgagagaa gcgaaggaag gcacagcaca
     6661 ggaggcatgg atgagctgta taaataagat atcgaattcg atatcaagct tatcgataat
     6721 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     6781 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     6841 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     6901 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     6961 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     7021 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     7081 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     7141 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     7201 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     7261 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     7321 cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     7381 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     7441 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     7501 aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
     7561 atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc
     7621 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta
     7681 gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg
     7741 gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac
     7801 atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc
     7861 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7921 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7981 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc
     8041 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     8101 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     8161 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     8221 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     8281 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     8341 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     8401 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     8461 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     8521 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     8581 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     8641 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     8701 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     8761 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     8821 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     8881 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     8941 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     9001 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     9061 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     9121 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     9181 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     9241 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     9301 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     9361 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     9421 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     9481 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     9541 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
     9601 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     9661 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     9721 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     9781 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     9841 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     9901 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     9961 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
    10021 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
    10081 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
    10141 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    10201 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    10261 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    10321 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    10381 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    10441 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    10501 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    10561 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    10621 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    10681 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
    10741 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    10801 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    10861 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    10921 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    10981 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    11041 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    11101 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    11161 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    11221 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    11281 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    11341 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    11401 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    11461 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    11521 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    11581 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    11641 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    11701 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    11761 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    11821 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    11881 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    11941 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    12001 catttccccg aaaagtgcca cctgac
//
