LOCUS       Exported                4689 bp ds-DNA     circular SYN 03-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Apr 21, 2022 from SnapGene 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        109..423
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    117..135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    153..171
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    188..206
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    224..242
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    260..278
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    295..313
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    331..349
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     404..442
                     /label=Fragment.REV
     promoter        428
                     /label=TRE promoter
                     /label=TRE\promoter
                     /note="/name=TRE promoter"
                     /note="/newname=TRE promoter"
                     /note="/subtype=inducible"
                     /note="/synthesis=False"
                     /note="/template=VF052-NKI764-VT-H7489"
                     /note="/vntifkey=30"
     CDS             429..2207
                     /codon_start=1
                     /gene="{u'gene_symbol': u'Rara', u'gene_seq': u'',
                     u'taxon': u'10090', u'gene_id': u'19401', u'strain': 
                     u'FVB', u'chr': u'11', u'transcript_id': u'', 
                     u'gene_ensembl': u'MGP_FVBNJ_G0018901', u'mrna_ensembl': 
                     u'MGP_FVBNJ_T0031990'}"
                     /label=Kozak-Mouse Rara-VP16
                     /label=Kozak-Mouse\Rara-VP16
                     /note="/cycle=1-2"
                     /note="/name=Kozak-Mouse Rara-VP16"
                     /note="/newname=Kozak-Mouse Rara-VP16"
                     /note="/price=314"
                     /note="/subtype="
                     /note="/synthesis=True"
                     /note="/template="
                     /note="/vntifkey=4"
                     /translation="ATMASNSSSCPTPGGGHLNGYPVPPYAFFFPPMLGGLSPPGALTS
                     LQHQLPVSGYSTPSPATIETQSSSSEEIVPSPPSPPPLPRIYKPCFVCQDKSSGYHYGV
                     SACEGCKGFFRRSIQKNMVYTCHRDKNCIINKVTRNRCQYCRLQKCFDVGMSKESVRND
                     RNKKKKEAPKPECSESYTLTPEVGELIEKVRKAHQETFPALCQLGKYTTNNSSEQRVSL
                     DIDLWDKFSELSTKCIIKTVEFAKQLPGFTTLTIADQITLLKAACLDILILRICTRYTP
                     EQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFANQLLPLEMDDAETGLLSAICLICGD
                     RQDLEQPDKVDMLQEPLLEALKVYVRKRRPSRPHMFPKMLMKITDLRSISAKGAERVIT
                     LKMEIPGSMPPLIQEMLENSEGLDTLSGQSGGGTRDGGGLAPPPGSCSPSLSPSSHRSS
                     PATQSPGPVRARTKNNYGSTIEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPP
                     TDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFE
                     FEQMFTDALGIDEYGG"
     primer_bind     complement(2188..2233)
                     /label=Fragment.FOR
     polyA_signal    2243..2364
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2783..3371)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3571..4431)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4536..4584
                     /note="synthetic polyadenylation signal"
     misc_feature    4598..4689
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt gcatgcctgc aggtcacgag gccctttcgt cttcactcga gtttactccc
      121 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgatgtcg
      181 agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga
      241 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttatcccta
      301 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
      361 ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa ccgtcagatc
      421 gcctggaggc caccatggcc agcaatagca gttcctgccc aacacctggg ggcgggcacc
      481 tcaatgggta cccagtaccc ccctacgcct tcttctttcc ccctatgctg ggtggactct
      541 ccccacccgg cgctctcacc agcctccagc accagcttcc agtcagtggt tacagcacac
      601 cgtccccagc caccatcgag acccagagca gcagttccga agagatagta cccagccctc
      661 cctcaccacc gcccctgccc cgcatctaca agccttgctt tgtttgtcaa gacaaatcat
      721 ccggctacca ctatggggtc agcgcctgtg agggctgtaa gggcttcttc cgacgaagca
      781 tccagaagaa catggtgtat acgtgtcacc gggacaagaa ctgcatcatc aacaaggtga
      841 cccggaaccg ctgccagtac tgccggctgc agaaatgttt cgacgtgggc atgtccaagg
      901 agtcggtgcg aaacgatcga aacaaaaaga agaaagaggc acccaagccc gagtgctcag
      961 agagctacac gctgacgcct gaggtgggcg agctcattga gaaggttcgc aaagcgcacc
     1021 aggagacctt cccggccctc tgccagctgg gcaagtacac tacgaacaac agctcagaac
     1081 aacgagtctc cctggacatt gacctctggg acaagttcag tgaactctcc accaagtgca
     1141 tcattaagac tgtggagttc gccaagcagc ttcccggctt caccaccctc accatcgccg
     1201 accagatcac cctcctcaag gctgcctgcc tggatatcct gattctgcga atctgcacgc
     1261 ggtacacgcc tgagcaagac acaatgacct tctcagatgg actgaccctg aaccggactc
     1321 agatgcacaa cgctggcttt ggccccctca ccgacttggt ctttgccttc gccaaccagc
     1381 tgctgcccct ggagatggac gatgctgaga ctggactgct cagtgccatc tgcctcatct
     1441 gtggagaccg acaggacctg gagcagccag acaaggtgga catgctgcaa gagccgctgc
     1501 tggaagcact gaaagtctac gtccggaaac ggaggcccag ccgaccccac atgttcccca
     1561 agatgctgat gaagatcaca gaccttcgga gcatcagcgc caagggagct gaacgggtga
     1621 tcacattgaa gatggagatc ccaggctcca tgccaccgct gatccaggaa atgctggaga
     1681 actctgaggg cttggacact ctaagcggac agtcgggggg cggaacacga gatgggggtg
     1741 gcctggcccc ccctccgggt agctgtagcc ccagcctcag tcccagctcc cacagaagca
     1801 gcccagccac ccaatcccca ggaccggtcc gcgcgcgtac gaaaaacaat tacgggtcta
     1861 ccatcgaggg cctgctcgat ctcccggacg acgacgcccc cgaagaggcg gggctggcgg
     1921 ctccgcgcct gtcctttctc cccgcgggac acacgcgcag actgtcgacg gcccccccga
     1981 ccgatgtcag cctgggggac gagctccact tagacggcga ggacgtggcg atggcgcatg
     2041 ccgacgcgct agacgatttc gatctggaca tgttggggga cggggattcc ccgggtccgg
     2101 gatttacccc ccacgactcc gccccctacg gcgctctgga tatggccgac ttcgagtttg
     2161 agcagatgtt taccgatgcc cttggaattg acgagtacgg tgggtaggag tcggggcggc
     2221 cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga
     2281 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     2341 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     2401 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc
     2461 gataaggatc cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg
     2521 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
     2581 aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2641 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2701 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2761 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2821 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2881 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2941 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3001 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3061 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3121 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3181 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3241 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3301 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3361 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3421 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3481 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3541 gcggccgcaa atgctaaacc actgcagtgg ttaccagtgc ttgatcagtg aggcaccgat
     3601 ctcagcgatc tgcctatttc gttcgtccat agtggcctga ctccccgtcg tgtagatcac
     3661 tacgattcgt gagggcttac catcaggccc cagcgcagca atgatgccgc gagagccgcg
     3721 ttcaccggcc cccgatttgt cagcaatgaa ccagccagca gggagggccg agcgaagaag
     3781 tggtcctgct actttgtccg cctccatcca gtctatgagc tgctgtcgtg atgctagagt
     3841 aagaagttcg ccagtgagta gtttccgaag agttgtggcc attgctactg gcatcgtggt
     3901 atcacgctcg tcgttcggta tggcttcgtt caactctggt tcccagcggt caagccgggt
     3961 cacatgatca cccatattat gaagaaatgc agtcagctcc ttagggcctc cgatcgttgt
     4021 cagaagtaag ttggccgcgg tgttgtcgct catggtaatg gcagcactac acaattctct
     4081 taccgtcatg ccatccgtaa gatgcttttc cgtgaccggc gagtactcaa ccaagtcgtt
     4141 ttgtgagtag tgtatacggc gaccaagctg ctcttgcccg gcgtctatac gggacaacac
     4201 cgcgccacat agcagtactt tgaaagtgct catcatcggg aatcgttctt cggggcggaa
     4261 agactcaagg atcttgccgc tattgagatc cagttcgata tagcccactc ttgcacccag
     4321 ttgatcttca gcatctttta ctttcaccag cgtttcgggg tgtgcaaaaa caggcaagca
     4381 aaatgccgca aagaagggaa tgagtgcgac acgaaaatgt tggatgctca tactcgtcct
     4441 ttttcaatat tattgaagca tttatcaggg ttactagtac gtctctcaag gataagtaag
     4501 taatattaag gtacgggagg tattggacag gccgcaataa aatatcttta ttttcattac
     4561 atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca
     4621 aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa
     4681 catttctct
//
