LOCUS       Exported                1829 bp DNA     linear   UNA 26-MAY-2015
DEFINITION  Human Galphai cDNA fused to EGFP.Inserted by Apa1-Spe1 in 
            pSP64-vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Mamiko
  TITLE     Direct Submission
  JOURNAL   Exported Apr 29, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..717
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagatg
      721 ccatgggggc cctcatgggc tgcacgctga gcgccgagga caaggcggcg gtggagcgga
      781 gtaagatgat cgaccgcaac ctccgtgagg acggcgagaa ggcggcgcgc gaggtcaagc
      841 tgctgctgct cggtgctggt gaatctggta aaagtacaat tgtgaagcag atgaaaatta
      901 tccatgaagc tggttattca gaagaggagt gtaaacaata caaagcagtg gtctacagta
      961 acaccatcca gtcaattatt gctatcatta gggctatggg gaggttgaag atagactttg
     1021 gtgactcagc ccgggcggat gatgcacgcc aactctttgt gctagctgga gctgctgaag
     1081 aaggctttat gactgcagaa cttgctggag ttataaagag attgtggaaa gatagtggtg
     1141 tacaagcctg tttcaacaga tcccgagagt accagcttaa tgattctgca gcatactatt
     1201 tgaatgactt ggacagaata gctcaaccaa attacatccc gactcaacaa gatgttctca
     1261 gaactagagt gaaaactaca ggaattgttg aaacccattt tactttcaaa gatcttcatt
     1321 ttaaaatgtt tgatgtggga ggtcagagat ctgagcggaa gaagtggatt cattgcttcg
     1381 aaggagtggc ggcgatcatc ttctgtgtag cactgagtga ctacgacctg gttctagctg
     1441 aagatgaaga aatgaaccga atgcatgaaa gcatgaaatt gtttgacagc atatgtaaca
     1501 acaagtggtt tacagataca tccattatac tttttctaaa caagaaggat ctttttgaag
     1561 aaaaaatcaa aaagagccct ctcactatat gctatcaaga atatgcagga tcaaacacat
     1621 atgaagaggc agctgcatat attcaatgtc agtttgaaga cctcaataaa agaaaggaca
     1681 caaaggaaat atacacccac ttcacatgtg ccacagatac taagaatgtg cagtttgttt
     1741 ttgatgctgt aacagatgtc atcataaaaa ataatctaaa agattgtggt ctctttggac
     1801 cgcgggcccg ggatccaccg gtcgccacc
//
