LOCUS       Exported                3139 bp DNA     linear   UNA 16-NOV-2017
DEFINITION  To construct pSP64TSN-mem-mCherry-Plk1 from Original plasmid pTK24: 
            Mem-mCherry-Plk1, Apa1 and Spe1 sites were used.
            
            n fusion
            cloning site for pSP64TSN vector- Apa1-Spe1: 
            atgccatgggggccctcgagactagtgactgacta 
            
             
            
            Primers:
            
            Mem-SP64-fusionF- atgccatgggggccc-atgctgtgctgtatgagaa
            
            Plk-SP64-fusionR -ccgtctcaaggcctcctaa–actagtgactgacta
            
             (primer: tagtcagtcactagtttaggaggccttgagacgg ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Mamiko
  TITLE     Direct Submission
  JOURNAL   Exported Apr 29, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3139
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1..64
                     /label=pSP64-vector sequence
     5'UTR           6..48
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     misc_feature    74..133
                     /label=membrane-targeted domain
     CDS             140..847
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent 
                     protein"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             926..970
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     misc_feature    1238..1240
                     /label=Lys -> Arg mutation in PLK1-Dead
     misc_feature    2807..3139
                     /label=pSP64-vector sequence
     primer_bind     complement(3080..3096)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcttgcttgt tctttttgca gaagctcaga ataaacgctc aactttggca gatctgatca
       61 tgccatgggg cccatgctgt gctgtatgag aagaaccaaa caggttgaaa aaaatgatga
      121 cgaccaaaag attggcggca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
      181 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
      241 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
      301 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
      361 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
      421 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
      481 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
      541 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
      601 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
      661 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
      721 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
      781 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
      841 gtacaagtcc ggaggggatc ggtggagctc caccggtggc ggccgctcta gagagaatct
      901 ttattttcag ggcgccgcca aattcaaaga aaccgctgct gctaaattcg aacgccagca
      961 catggacagc ggaggtggag gtactagtct cgagatgagt gctgcagtga ctgcagggaa
     1021 gctggcacgg gcaccggccg accctgggaa agccggggtc cccggagttg cagctcccgg
     1081 agctccggcg gcggctccac cggcgaaaga gatcccggag gtcctagtgg acccacgcag
     1141 ccggcggcgc tatgtgcggg gccgcttttt gggcaagggc ggctttgcca agtgcttcga
     1201 gatctcggac gcggacacca aggaggtgtt cgcgggcaag attgtgccta agtctctgct
     1261 gctcaagccg caccagaggg agaagatgtc catggaaata tccattcacc gcagcctcgc
     1321 ccaccagcac gtcgtaggat tccacggctt tttcgaggac aacgacttcg tgttcgtggt
     1381 gttggagctc tgccgccgga ggtctctcct ggagctgcac aagaggagga aagccctgac
     1441 tgagcctgag gcccgatact acctacggca aattgtgctt ggctgccagt acctgcaccg
     1501 aaaccgagtt attcatcgag acctcaagct gggcaacctt ttcctgaatg aagatctgga
     1561 ggtgaaaata ggggattttg gactggcaac caaagtcgaa tatgacgggg agaggaagaa
     1621 gaccctgtgt gggactccta attacatagc tcccgaggtg ctgagcaaga aagggcacag
     1681 tttcgaggtg gatgtgtggt ccattgggtg tatcatgtat accttgttag tgggcaaacc
     1741 accttttgag acttcttgcc taaaagagac ctacctccgg atcaagaaga atgaatacag
     1801 tattcccaag cacatcaacc ccgtggccgc ctccctcatc cagaagatgc ttcagacaga
     1861 tcccactgcc cgcccaacca ttaacgagct gcttaatgac gagttcttta cttctggcta
     1921 tatccctgcc cgtctcccca tcacctgcct gaccattcca ccaaggtttt cgattgctcc
     1981 cagcagcctg gaccccagca accggaagcc cctcacagtc ctcaataaag gcttggagaa
     2041 ccccctgcct gagcgtcccc gggaaaaaga agaaccagtg gttcgagaga caggtgaggt
     2101 ggtcgactgc cacctcagtg acatgctgca gcagctgcac agtgtcaatg cctccaagcc
     2161 ctcggagcgt gggctggtca ggcaagagga ggctgaggat cctgcctgca tccccatctt
     2221 ctgggtcagc aagtgggtgg actattcgga caagtacggc cttgggtatc agctctgtga
     2281 taacagcgtg ggggtgctct tcaatgactc aacacgcctc atcctctaca atgatggtga
     2341 cagcctgcag tacatagagc gtgacggcac tgagtcctac ctcaccgtga gttcccatcc
     2401 caactccttg atgaagaaga tcaccctcct taaatatttc cgcaattaca tgagcgagca
     2461 cttgctgaag gcaggtgcca acatcacgcc gcgcgaaggt gatgagctcg cccggctgcc
     2521 ctacctacgg acctggttcc gcacccgcag cgccatcatc ctgcacctca gcaacggcag
     2581 cgtgcagatc aacttcttcc aggatcacac caagctcatc ttgtgcccac tgatggcagc
     2641 cgtgacctac atcgacgaga agcgggactt ccgcacatac cgcctgagtc tcctggagga
     2701 gtacggctgc tgcaaggagc tggccagccg gctccgctac gcccgcacta tggtggacaa
     2761 gctgctgagc tcacgctcgg ccagcaaccg tctcaaggcc tcctaactag tgactgacta
     2821 ggatctggtt accactaaac cagcctcaag aacacccgaa tggagtctct aagctacata
     2881 ataccaactt acactttaca aaatgttgtc cccccaaaat gtagccattc gtatctgctc
     2941 ctaataaaaa agaaagtttc ttcacattct aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3001 cccccccccc cccccccccc cccccccccc tggaggtcga ctctanagga tccccgggcg
     3061 agctcgaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat cccgctcaca
     3121 attccncaca acatacgag
//
