LOCUS       Exported                2954 bp DNA     linear   UNA 26-MAY-2017
DEFINITION  ePDZb1 was inserted at Not1 site of AGS-GFP
            
            MiniSOG was inserted at APA1 site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Apr 29, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1..761
                     /label=Upstream region seqed by Kn555 & Kn554
     primer_bind     725..749
                     /label=IVT-SP6.f1
     primer_bind     731..759
                     /label=IVT-SP6.f2
     5'UTR           767..809
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     primer_bind     808..853
                     /label=miniSOG_GFPf
     misc_feature    824
                     /label=Change to C to remove another ORF
     misc_feature    826
                     /label=Sequencing insertion
     CDS             832..1149
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     primer_bind     complement(1132..1177)
                     /label=miniSOG_GFPr
     CDS             1156..1872
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1156..1872
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     1641..1663
                     /label=eGFP-Cf1
     primer_bind     1680..1698
                     /label=eGFP-Cf2
     primer_bind     1890..1931
                     /label=InFus-ePDZf
     misc_feature    1912..2493
                     /label=ePDZb1
     misc_feature    2500..2535
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(2515..2556)
                     /label=InFus-ePDZr
     CDS             2550..2570
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2583..2627
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(2737..2762)
                     /label=Vasa reverse sequencing primer 1
     primer_bind     complement(2859..2882)
                     /label=B-globinR4-2
     primer_bind     complement(2875..2898)
                     /label=B-globinR4
     primer_bind     complement(2893..2916)
                     /label=M13R
     primer_bind     complement(2896..2912)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2909..2932)
                     /label=M13R0
ORIGIN
        1 cattaaccta taaaaaatag gcgtatcacg aggccctttt cgtctcgcgc gtttcggtga
       61 tgacggtgaa aacntntgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
      121 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
      181 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccatt cgacgctctc
      241 ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg
      301 ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc
      361 ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt
      421 ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc
      481 cggccacgat gcgtccggcg tagaggatct ggctagcgat gaccctgctg attggttcgc
      541 tgaccatttc cgggtgcggg acggcgttac cagaaactca gaaggttcgt ccaaccaaac
      601 cgactctgac ggcagtttac gagagagatg atagggtctg cttcagtaag ccagatgcta
      661 cacaattagg cttgtacata ttgtcgttag aacgcggcta caattaatac ataaccttat
      721 gtatcataca catacgattt aggtgacact atagaataca agcttgcttg ttctttttgc
      781 agaagctcag aataaacgct caactttggc agatctatgc catcggggcc catggagaaa
      841 agtttcgtga taactgatcc acggctgcca gacaatccca tcatcttcgc atccgatggc
      901 ttcctggagc tgaccgagta ttccagagag gagatcctgg gccgcaatgg ccgctttctg
      961 cagggaccag agacagacca ggccacagtg cagaagattc gcgatgccat tagagatcag
     1021 cgcgagatta ccgtgcagct gataaactac acaaaaagcg ggaagaaatt ctggaacctc
     1081 ctgcacctcc agcccatgag ggaccagaag ggtgagctcc agtatttcat cggagtgcag
     1141 ctggatggag ggcccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     1201 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     1261 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     1321 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     1381 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     1441 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     1501 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     1561 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     1621 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     1681 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     1741 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     1801 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     1861 gagctataca agtccggagg ggatcggtgg agctccaccg gtggcggccg catgccagaa
     1921 cttggattta gcatatcagg tggtgtcggg ggtagaggaa acccattcag acctgatgat
     1981 gatggtatat ttgtaacaag ggtacaacct gaaggaccag catcaaaatt actgcagcca
     2041 ggtgataaaa ttattcaggc taatggctac agttttataa atattgaaca tggacaagca
     2101 gtgtccttgc taaaaacttt ccagaataca gttgaactca tcattgtacg agaagttggt
     2161 aacggtgcaa aacaagagat tcgagtgagg gttgaaaaag acggtggttc tggaggcgtt
     2221 tcttctgttc cgaccaatct ggaagttgtt gctgcgaccc cgactagcct gctgatcagc
     2281 tgggatgcgt atagggagct tccggttagt tattaccgta tcacgtacgg tgaaaccggt
     2341 ggtaactccc cggttcagga attcactgta cctggttcca agtctactgc taccatcagc
     2401 ggcctgaaac cgggtgtcga ctataccatc actgtatacg cacattacaa ctaccattac
     2461 tactctagcc caatctcgat taactaccgt acctctagac tcgagctcaa gcttcgaatt
     2521 ctgcagtcga cggtagcggc cgctctagag agaatcttta ttttcagggc gccgccaaat
     2581 tcaaagaaac cgctgctgct aaattcgaac gccagcacat ggacagcgga ggtggaggta
     2641 ctagtgactg actaggatct ggttaccact aaaccagcct caagaacacc cgaatggagt
     2701 ctctaagcta cataatacca acttacactt tacaaaatgt tgtcccccaa aatgtagcca
     2761 ttcgtatctg ctcctaataa aaagaaagtt tcttcacatt ctaaaaaaaa aaaaaaaaaa
     2821 aaaacccccc cccccccccc cccccctgca ggtcgactct agaggatccc cgggcgagct
     2881 cgaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     2941 acacaacata cgag
//
