LOCUS       Exported                4074 bp DNA     linear   UNA 08-MAR-2019
DEFINITION  KP-057Kaede reverse primer 3 
            tagtcagtcactagGggccccCTTGACGTTGTCCGGCAATC JKP-058Kaede forward 
            primer 4 tctgatcatgccatggggcccATGGTGAGTCTGATTAAAC 
            
            
            JKP-059Vasa C-term deletion 2 reverse primer 
            ccagatcctagtcagtcactagGggcccTCCGACACGTCCGGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Apr 29, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1..761
                     /label=Upstream region seqed by Kn555 & Kn554
     primer_bind     725..749
                     /label=IVT-SP6.f1
     primer_bind     731..759
                     /label=IVT-SP6.f2
     promoter        737..755
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     5'UTR           767..809
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     primer_bind     814..854
                     /label=JKP-058
     misc_feature    836..1513
                     /label=Kaede
     primer_bind     complement(1494..1520)
                     /label=JKP-057
     primer_bind     complement(1494..1513)
                     /label=KaedeR-x
     misc_feature    1523..3729
                     /label=Vasa
     misc_feature    1523..1945
                     /label=1F
     primer_bind     1523..1543
                     /label=Dendra2-VasaIF3R forward primer
     primer_bind     1524..1548
                     /label=Vasa forward sequencing primer 1
     primer_bind     complement(1541..1562)
                     /label=Photoconvertible protein Vasa R sequencing primer
     misc_feature    1946..2131
                     /label=2F
     primer_bind     1997..2021
                     /label=VasaORF2Ff
     primer_bind     2127..2150
                     /label=VasaORF4Ff
     misc_feature    2132..3181
                     /label=4F
     misc_feature    2222..2314
                     /label=PEST region
     primer_bind     complement(2423..2446)
                     /label=VasaORF-4Fr2
     primer_bind     complement(2462..2486)
                     /label=VasaORF-4Fr1
     primer_bind     2630..2653
                     /label=gRNA-Vasa2f
     primer_bind     2737..2761
                     /label=Vasa forward sequencing primer 2
     misc_feature    2840..2851
                     /label=DEAD box
     primer_bind     complement(3104..3127)
                     /label=gRNA-Vasa3r
     misc_feature    3107..3109
                     /label=R2361G
     misc_feature    3182..3729
                     /label=6F
     primer_bind     complement(3395..3409)
                     /label=JKP-059
     misc_feature    3419..3729
                     /label=Vasa-3R (removed for Vasa-dead construct)
     primer_bind     complement(3979..4002)
                     /label=B-globinR4-2
     primer_bind     complement(3995..4018)
                     /label=B-globinR4
     primer_bind     complement(4013..4036)
                     /label=M13R
     primer_bind     complement(4016..4032)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4029..4052)
                     /label=M13R0
ORIGIN
        1 cattaaccta taaaaaatag gcgtatcacg aggccctttt cgtctcgcgc gtttcggtga
       61 tgacggtgaa aacntntgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
      121 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
      181 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccatt cgacgctctc
      241 ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg
      301 ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc
      361 ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt
      421 ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc
      481 cggccacgat gcgtccggcg tagaggatct ggctagcgat gaccctgctg attggttcgc
      541 tgaccatttc cgggtgcggg acggcgttac cagaaactca gaaggttcgt ccaaccaaac
      601 cgactctgac ggcagtttac gagagagatg atagggtctg cttcagtaag ccagatgcta
      661 cacaattagg cttgtacata ttgtcgttag aacgcggcta caattaatac ataaccttat
      721 gtatcataca catacgattt aggtgacact atagaataca agcttgcttg ttctttttgc
      781 agaagctcag aataaacgct caactttggc agatctgatc atgccatggg ggcccatggt
      841 gagtctgatt aaaccagaaa tgaagatcaa gctgcttatg gaaggcaatg taaacgggca
      901 ccagtttgtt attgagggag atggaaaagg ccatcctttt gagggaaaac agagtatgga
      961 ccttgtagtc aaagaaggcg cacctctccc ttttgcctac gatatcttga caacagcatt
     1021 ccattatggt aacagggttt ttgctaaata cccagaccat ataccagact acttcaagca
     1081 gtcgtttccc aaagggtttt cttgggagcg aagcctgatg ttcgaggacg ggggcgtttg
     1141 catcgctaca aatgacataa cactgaaagg agacactttt tttaacaaag ttcgatttga
     1201 tggcgtaaac tttcccccaa atggtcctgt tatgcagaag aagactctga aatgggaggc
     1261 atccactgag aaaatgtatt tgcgtgatgg agtgttgacg ggcgatatta caatggctct
     1321 gctgcttaaa ggagatgtcc attaccgatg tgacttcaga actacttaca aatctaggca
     1381 ggagggtgtc aagttgccag gatatcactt tgtcgatcac tgcatcagca tattgaggca
     1441 tgacaaagac tacaacgagg ttaagctgta tgagcacgct gttgcccatt ctggattgcc
     1501 ggacaacgtc aagggggccc aaatgtcaga agactggggg acctcttcgg tgtcgggtgg
     1561 atccagcttt ggaaagagta gttttggtgg atcaagtggt ggggtcaaag acgatggatt
     1621 tggcgttgga gtcggtcgag gtcgaggaag gggtcgcggg tttagcccct ttgcttcaaa
     1681 cggtggtgtc ggtggtatga ccaatgggac taccaatgga ggaggagaga gtagcggtgg
     1741 gtgggatgag agcccatcta agagtggctc aagtagcagt ccatggggcg gtgatggtga
     1801 taatgggttc ggcaagagta gctttggtag caaatcgttc ggtggaggag gaggaggtgg
     1861 tggtggtggg ggccgaggtg ctgaatcagg aggaggacga ggaggaggag gattcggcgg
     1921 cagacgagga ggacgtggag gtggagatag atcctgttat aactgcggtg aaacaggtca
     1981 catgtcaaga gaatgtccca caaaggactc ttcaggagga ggacgtggtg gtggtggtgg
     2041 cgatagaagt tgctacaatt gcggtgagac cggacacatg tcacgagaat gtcccacaaa
     2101 agactcttcg ggtggaggag gtggaggtgg tggaaagtgc tttagatgtc aagaagaagg
     2161 ccactttgcc aaggaatgcc ccaatccaga tacaagaaat gaaggcgaag gtggtgaagc
     2221 caagccgcca gcatccacct acgttcctcc cgacccatcc gaggaagaat cacagattta
     2281 cataagcact gaacaaggaa tcaacttcaa caagtatgat gatatacccg tagaagtcag
     2341 cggtcgagac aggccaaaaa atatacgagc ttttgaggag gccgagctag atgagacggt
     2401 attgtccaat gtgcgcaagg ctcactacgc taagccaaca cctgtgcaga agtatggtat
     2461 gcccatcatt tcttgtggac gtgatctgat ggcctgtgcc caaacaggat caggaaagac
     2521 ggcggcattc ctcctaccca tcatcaccaa catgatcacc cacggaggat gcatcagcac
     2581 cttcaacgtg atccaggaac cactggccct catcgtctcg ccaacccgcg agctggccat
     2641 ccagatctac aacgaggcac gcaagttctg ccgcggcacc accatacgac ctgtggtggt
     2701 atacggaggc accagcgtca gtcatcagat gagagagtgt cagaagggat gctccatcct
     2761 ggtagccacc ccaggacgta tgcatgactt cgtaaccaga ggatatatcg gcctgggcaa
     2821 gctgaagttc ctgatccttg acgaagccga ccgtatgttg gacatgggtt tcggtcccga
     2881 catccagaga ctgatcgagc accctcacat gcctgccaag ggtgatcgtc aaaccctgat
     2941 gttcagtgcg accttcccca atgaggtcca ggagaaggca ggggagtacc tcaacgacta
     3001 cctcttcctg acggtaggga gggtaggagg agctgcctca gacatcgagc agagggtgtt
     3061 ctcggtcggt caattcgaca agcgggataa gctgatggag atcctggggg atcagaaaga
     3121 tgatgaccgt actctggtgt ttgtttcgac caagaggaat gctgatttct tggcctctct
     3181 tctcagtcaa tctgaattcc ccaccaccag catccatggt gaccgtcagc agcaggagcg
     3241 tgaggaagcc ctgcgtgact tcaagaccgg caaggcacca atccttgttg ccacgtccgt
     3301 cgccgccaga ggtctggaca tcccaggggt caagcacgtg gtcaactacg acctcccaag
     3361 cgacatcgat gagtacgtgc atcgcatcgg gcgtaccgga cgtgtcggaa accttggacg
     3421 atcaactagc ttctatgatg cagaaaagga tgctaacatt gcgagagctc tcattaagat
     3481 cttggctgat gcgcagcagg aagtaccgga gttcttagag gaggctgctg attcagccat
     3541 tggaacctac catggcaacg ctggagggag ctttggaggc agggacacca ggaagttcgg
     3601 tggtcgagga ggaggaaggg gaggaggaag tggcggcggt ggtggagacg agtggggatc
     3661 tggaggagga ggcggcggcg gtggcggagg aggagctgct gatgatgaat catgggatta
     3721 gctcgagtag ggccctcgag actagtgact gactaggatc tggttaccac taaaccagcc
     3781 tcaagaacac ccgaatggag tctctaagct acataatacc aacttacact ttacaaaatg
     3841 ttgtccccca aaatgtagcc attcgtatct gctcctaata aaaagaaagt ttcttcacat
     3901 tctaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaacccc cccccccccc cccccccccc
     3961 cccccctgca ggtcgactct agaggatccc cgggcgagct cgaattcgta atcatgtcat
     4021 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgag
//
