LOCUS       Exported                3886 bp DNA     linear   UNA 07-NOV-2016
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Apr 29, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1..761
                     /label=Upstream region seqed by Kn555 & Kn554
     primer_bind     725..749
                     /label=IVT-SP6.f1
     primer_bind     731..759
                     /label=IVT-SP6.f2
     promoter        737..755
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     5'UTR           767..809
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     primer_bind     824..857
                     /label=Dendra-2 forward primer 3
     misc_feature    830..833
                     /label=Sequencing insertion
     CDS             839..1528
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     primer_bind     complement(1517..1555)
                     /label=Dendra-2 reverse primer 4
     primer_bind     1520..1558
                     /label=Dendra2-VasaIF3R forward primer
     misc_feature    1538..3744
                     /label=Vasa
     primer_bind     1539..1563
                     /label=Vasa forward sequencing primer 1
     primer_bind     complement(1556..1577)
                     /label=Photoconvertible protein Vasa R sequencing primer
     primer_bind     2752..2776
                     /label=Vasa forward sequencing primer 2
     misc_feature    3122..3124
                     /label=R2361G
     primer_bind     complement(3840..3865)
                     /label=Vasa reverse sequencing primer 1
ORIGIN
        1 cattaaccta taaaaaatag gcgtatcacg aggccctttt cgtctcgcgc gtttcggtga
       61 tgacggtgaa aacntntgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
      121 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
      181 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccatt cgacgctctc
      241 ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg
      301 ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc
      361 ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt
      421 ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc
      481 cggccacgat gcgtccggcg tagaggatct ggctagcgat gaccctgctg attggttcgc
      541 tgaccatttc cgggtgcggg acggcgttac cagaaactca gaaggttcgt ccaaccaaac
      601 cgactctgac ggcagtttac gagagagatg atagggtctg cttcagtaag ccagatgcta
      661 cacaattagg cttgtacata ttgtcgttag aacgcggcta caattaatac ataaccttat
      721 gtatcataca catacgattt aggtgacact atagaataca agcttgcttg ttctttttgc
      781 agaagctcag aataaacgct caactttggc agatctgatc atgccatggt tcgggcccat
      841 gaacaccccg ggaattaacc tgatcaagga ggacatgcgc gtgaaggtgc acatggaggg
      901 caacgtgaac ggccacgcct tcgtgatcga gggcgagggc aagggcaagc cctacgaggg
      961 cacccagacc gccaacctga ccgtgaagga gggcgccccc ctgcccttca gctacgacat
     1021 cctgaccacc gccgtgcact acggcaaccg ggtgttcacc aagtaccccg aggacatccc
     1081 cgactacttc aagcagagct tccccgaggg ctacagctgg gagcgcacca tgaccttcga
     1141 ggacaagggc atctgcacca tccgcagcga catcagcctg gagggcgact gcttcttcca
     1201 gaacgtgcgc ttcaagggca ccaacttccc ccccaacggc cccgtgatgc agaagaagac
     1261 cctgaagtgg gagcccagca ccgagaagct gcacgtgcgc gacggcctgc tggtgggcaa
     1321 catcaacatg gccctgctgc tggagggcgg cggccactac ctgtgcgact tcaagaccac
     1381 ctacaaggcc aagaaggtgg tgcagctgcc cgacgcccac ttcgtggacc accgcatcga
     1441 gatcctgggc aacgacagcg actacaacaa ggtgaagctg tacgagcacg ccgtggcccg
     1501 ctacagcccc ctgcccagcc aggtgtggaa ggggcccatg tcagaagact gggggacctc
     1561 ttcggtgtcg ggtggatcca gctttggaaa gagtagtttt ggtggatcaa gtggtggggt
     1621 caaagacgat ggatttggcg ttggagtcgg tcgaggtcga ggaaggggtc gcgggtttag
     1681 cccctttgct tcaaacggtg gtgtcggtgg tatgaccaat gggactacca atggaggagg
     1741 agagagtagc ggtgggtggg atgagagccc atctaagagt ggctcaagta gcagtccatg
     1801 gggcggtgat ggtgataatg ggttcggcaa gagtagcttt ggtagcaaat cgttcggtgg
     1861 aggaggagga ggtggtggtg gtgggggccg aggtgctgaa tcaggaggag gacgaggagg
     1921 aggaggattc ggcggcagac gaggaggacg tggaggtgga gatagatcct gttataactg
     1981 cggtgaaaca ggtcacatgt caagagaatg tcccacaaag gactcttcag gaggaggacg
     2041 tggtggtggt ggtggcgata gaagttgcta caattgcggt gagaccggac acatgtcacg
     2101 agaatgtccc acaaaagact cttcgggtgg aggaggtgga ggtggtggaa agtgctttag
     2161 atgtcaagaa gaaggccact ttgccaagga atgccccaat ccagatacaa gaaatgaagg
     2221 cgaaggtggt gaagccaagc cgccagcatc cacctacgtt cctcccgacc catccgagga
     2281 agaatcacag atttacataa gcactgaaca aggaatcaac ttcaacaagt atgatgatat
     2341 acccgtagaa gtcagcggtc gagacaggcc aaaaaatata cgagcttttg aggaggccga
     2401 gctagatgag acggtattgt ccaatgtgcg caaggctcac tacgctaagc caacacctgt
     2461 gcagaagtat ggtatgccca tcatttcttg tggacgtgat ctgatggcct gtgcccaaac
     2521 aggatcagga aagacggcgg cattcctcct acccatcatc accaacatga tcacccacgg
     2581 aggatgcatc agcaccttca acgtgatcca ggaaccactg gccctcatcg tctcgccaac
     2641 ccgcgagctg gccatccaga tctacaacga ggcacgcaag ttctgccgcg gcaccaccat
     2701 acgacctgtg gtggtatacg gaggcaccag cgtcagtcat cagatgagag agtgtcagaa
     2761 gggatgctcc atcctggtag ccaccccagg acgtatgcat gacttcgtaa ccagaggata
     2821 tatcggcctg ggcaagctga agttcctgat ccttgacgaa gccgaccgta tgttggacat
     2881 gggtttcggt cccgacatcc agagactgat cgagcaccct cacatgcctg ccaagggtga
     2941 tcgtcaaacc ctgatgttca gtgcgacctt ccccaatgag gtccaggaga aggcagggga
     3001 gtacctcaac gactacctct tcctgacggt agggagggta ggaggagctg cctcagacat
     3061 cgagcagagg gtgttctcgg tcggtcaatt cgacaagcgg gataagctga tggagatcct
     3121 gggggatcag aaagatgatg accgtactct ggtgtttgtt tcgaccaaga ggaatgctga
     3181 tttcttggcc tctcttctca gtcaatctga attccccacc accagcatcc atggtgaccg
     3241 tcagcagcag gagcgtgagg aagccctgcg tgacttcaag accggcaagg caccaatcct
     3301 tgttgccacg tccgtcgccg ccagaggtct ggacatccca ggggtcaagc acgtggtcaa
     3361 ctacgacctc ccaagcgaca tcgatgagta cgtgcatcgc atcgggcgta ccggacgtgt
     3421 cggaaacctt ggacgatcaa ctagcttcta tgatgcagaa aaggatgcta acattgcgag
     3481 agctctcatt aagatcttgg ctgatgcgca gcaggaagta ccggagttct tagaggaggc
     3541 tgctgattca gccattggaa cctaccatgg caacgctgga gggagctttg gaggcaggga
     3601 caccaggaag ttcggtggtc gaggaggagg aaggggagga ggaagtggcg gcggtggtgg
     3661 agacgagtgg ggatctggag gaggaggcgg cggcggtggc ggaggaggag ctgctgatga
     3721 tgaatcatgg gattagctcg agtactagtg actgactagg atctggttac cactaaacca
     3781 gcctcaagaa cacccgaatg gagtctctaa gctacataat accacttaca ctttacaaaa
     3841 tgttgtcccc caaaatgtag ccattcgtat ctgctcctaa taaaaa
//
