LOCUS       eNme2-T.1-ABE8e-bpNLS        7356 bp    DNA     circular UNA 26-APR-2022
DEFINITION  pBR322 ori.
ACCESSION   urn.local...x04-8letjr8
VERSION     urn.local...x04-8letjr8
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     primer_bind     219..243
                     /standard_name="BM244"
     primer_bind     234..253
                     /standard_name="LCv2_seq1"
     primer_bind     261..285
                     /standard_name="ACK15"
     primer_bind     261..285
                     /standard_name="JLseq007_ACK15"
     regulatory      275..356
                     /note="Geneious type: Promoter"
                     /standard_name="CMV prom"
     primer_bind     286..306
                     /standard_name="Genewiz CMV-Forward"
     primer_bind     286..306
                     /standard_name="Eton CMV-Forward"
     primer_bind     286..306
                     /standard_name="QB0012 CMV Forward"
     primer_bind     293..314
                     /standard_name="QB0976 pRLuc-MCS-F"
     primer_bind     complement(331..360)
                     /standard_name="352_CMV_Rev2"
     regulatory      377..395
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     misc_feature    403..408
                     /note="Geneious type: motif"
                     /standard_name="Kozak sequence"
     CDS             445..465
                     /standard_name="SV40 nLS "
     CDS             466..963
                     /standard_name="TadAmax10 (ABE8)"
     primer_bind     complement(946..985)
                     /standard_name="TPH1606"
     CDS             964..1059
                     /standard_name="Genscript BE4 linker"
     misc_feature    972..985
                     /standard_name="USER Junction"
     primer_bind     972..991
                     /standard_name="TPH1605"
     primer_bind     complement(1047..1091)
                     /standard_name="TPH1658"
     source          <1060..>4299
     source          <1060..>4299
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     CDS             1060..4299
                     /standard_name="nNmeCas9"
     misc_feature    1081..1091
                     /standard_name="USER Junction"
     primer_bind     1081..1122
                     /standard_name="TPH1505"
     modified_base   1102..1104
     primer_bind     1105..1129
                     /standard_name="Nme Seq 1"
     primer_bind     complement(1118..1158)
                     /standard_name="TPH1653"
     misc_feature    1148..1158
                     /standard_name="USER Junction"
     primer_bind     1148..1167
                     /standard_name="TPH1652"
     modified_base   1195..1197
     misc_feature    1195
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="G"
     modified_base   1258..1260
     misc_feature    1258
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="G"
     modified_base   1423..1425
     misc_feature    1423
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="A"
     modified_base   1510..1512
     misc_feature    1511
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="A"
     modified_base   1516..1518
     misc_feature    1516
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="G"
     primer_bind     1707..1731
                     /standard_name="Nme Seq 2"
     modified_base   2242..2244
     misc_feature    2242
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="A"
     primer_bind     2246..2270
                     /standard_name="Nme Seq 3"
     modified_base   2296..2298
     misc_feature    2296
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="C"
     modified_base   2335..2337
     misc_feature    2335
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="G"
     modified_base   2410..2412
     misc_feature    2411
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="A"
     modified_base   2434..2436
     misc_feature    2435
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="A"
     modified_base   2506..2508
     misc_feature    2506
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="G"
     primer_bind     2757..2781
                     /standard_name="Nme Seq 4"
     primer_bind     complement(2799..2825)
                     /standard_name="TPH1659"
     misc_feature    2810..2825
                     /standard_name="USER Junction"
     primer_bind     2810..2837
                     /standard_name="TPH1660"
     modified_base   2818..2820
     modified_base   2941..2943
     misc_feature    2941
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="T"
     modified_base   3076..3078
     misc_feature    3077
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="A"
     misc_feature    3085
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="C"
     modified_base   3214..3216
     misc_feature    3215
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="A"
     misc_feature    3243
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="G"
     primer_bind     3285..3309
                     /standard_name="Nme Seq 5"
     modified_base   3349..3351
     misc_feature    3350
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="T"
     modified_base   3355..3357
     misc_feature    3355
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="C"
     modified_base   3367..3369
     misc_feature    3368
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="A"
     modified_base   3517..3519
     misc_feature    3518
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="T"
     misc_feature    3581..3593
                     /standard_name="USER Junction"
     modified_base   3586..3588
     misc_feature    3587
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="A"
     modified_base   3631..3633
     misc_feature    3631
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="A"
     modified_base   3649..3651
     misc_feature    3650
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="G"
     modified_base   3907..3909
     misc_feature    3908
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="T"
     modified_base   4069..4071
     misc_feature    4070
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="A"
     modified_base   4138..4140
     misc_feature    4138
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="G"
     modified_base   4141..4143
     misc_feature    4141
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="T"
     modified_base   4153..4155
     misc_feature    4153..4155
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AGG"
     modified_base   4201..4203
     misc_feature    4201
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="C"
     modified_base   4246..4248
     misc_feature    4247
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="A"
     primer_bind     complement(4284..4298)
                     /standard_name="TPH1662"
     misc_feature    4286..4298
                     /standard_name="USER Junction"
     primer_bind     4286..4319
                     /standard_name="TPH1661"
     source          <4300..4311
     CDS             4312..4362
                     /standard_name="BPNLS"
     primer_bind     complement(4411..4428)
                     /standard_name="Eton BGH Reverse"
     regulatory      4414..4641
                     /note="Geneious type: Terminator"
                     /standard_name="bGH_PA term"
     misc_feature    complement(4563..4587)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="ACK64"
     primer_bind     complement(4563..4587)
                     /standard_name="ACK64 (end)"
     primer_bind     complement(4712..4728)
                     /standard_name="Eton M13R Reverse"
     primer_bind     complement(4725..4747)
                     /standard_name="QB0050 M13/pUC Reverse"
     regulatory      complement(4761..4790)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     misc_feature    complement(4805..4829)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="ACK32"
     primer_bind     4805..4829
                     /standard_name="ACK21"
     misc_feature    complement(4837..4853)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="BM524"
     misc_feature    complement(5099..5718)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
     misc_feature    complement(5106..5129)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="BM317"
     primer_bind     5114..5138
                     /standard_name="ACK10"
     misc_feature    5310
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    5555
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="G"
     primer_bind     5615..5639
                     /standard_name="ACK11"
     misc_feature    complement(5873..6733)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     primer_bind     6114..6138
                     /standard_name="ACK12"
     primer_bind     6279..6298
                     /standard_name="ACK563"
     primer_bind     6496..6515
                     /standard_name="QB0005 Amp-R"
     primer_bind     6581..6608
                     /standard_name="563_Amp_Rev"
     primer_bind     complement(6581..6608)
                     /standard_name="562_Amp_Fwd"
     primer_bind     6614..6638
                     /standard_name="ACK13"
     regulatory      complement(6775..6803)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     primer_bind     6847..6864
                     /standard_name="QB0090 pQE promoter"
     primer_bind     complement(6917..6936)
                     /standard_name="QB0092 pRS-marker"
     primer_bind     7082..7102
                     /standard_name="BM525/KZ887"
     primer_bind     7117..7141
                     /standard_name="ACK14"
     primer_bind     7117..7141
                     /standard_name="JLseq006_ACK14"
     regulatory      7124..356
                     /note="Geneious type: promoter"
                     /standard_name="CMV_Full"
     primer_bind     7124..7167
                     /standard_name="387_pAAV_fwd"
     primer_bind     7168..7185
                     /standard_name="BM243"
     primer_bind     complement(7174..7193)
                     /standard_name="QB1110 pSicoR"
     misc_feature    7313
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="C"
ORIGIN      
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctctga ggtggagttt
      481 tcccacgagt actggatgag acatgccctg accctggcca agagggcacg ggatgagagg
      541 gaggtgcctg tgggagccgt gctggtgctg aacaatagag tgatcggcga gggctggaac
      601 agagccatcg gcctgcacga cccaacagcc catgccgaaa ttatggccct gagacagggc
      661 ggcctggtca tgcagaacta cagactgatt gacgccaccc tgtacgtgac attcgagcct
      721 tgcgtgatgt gcgccggcgc catgatccac tctaggatcg gccgcgtggt gtttggcgtg
      781 aggaactcaa aaagaggcgc cgcaggctcc ctgatgaacg tgctgaacta ccccggcatg
      841 aatcaccgcg tcgaaattac cgagggaatc ctggcagatg aatgtgccgc cctgctgtgc
      901 gatttctatc ggatgcctag acaggtgttc aatgctcaga agaaggccca gagctccatc
      961 aactctggag gatctagcgg aggatcctct ggcagcgaga caccaggaac aagcgagtca
     1021 gcaacaccag agagcagtgg cggcagcagc ggcggcagcg cagcattcaa gcctaaccca
     1081 atcaattaca tcctgggact ggcaatcgga atcgcatccg tgggatgggc tatggtggag
     1141 atcgacgagg aggagaatcc tatccggctg atcgatctgg gcgtgagagt gtttaagagg
     1201 gccgaggtgc caaagaccgg cgattctctg gctatggccc ggagactggc acggagcatg
     1261 aggcgcctga cacggagaag ggcacacagg ctgctgaggg cacgccggct gctgaagaga
     1321 gagggcgtgc tgcaggcagc agacttcgat gagaatggcc tgatcaagag cctgccgaac
     1381 accccttggc agctgagagc agccgccctg gacaggaagc tggcaccact ggagtggtct
     1441 gccgtgctgc tgcacttgat caagcaccgc ggctacctga gccagcggaa gaacgaggga
     1501 gagacagcag gcaagaagct gggcgccctg ctgaagggag tggccaacaa tgcccacgcc
     1561 ctgcagaccg gcgatttcag gacacctgcc gagctggccc tgaataagtt tgagaaggag
     1621 tccggccaca tcagaaacca gaggggcgac tatagccaca ccttctcccg caaggatctg
     1681 caggccgagc tgatcctgct gttcgagaag cagaaggagt ttggcaatcc acacgtgagc
     1741 ggaggcctga aggagggaat cgagaccctg ctgatgacac agaggcctgc cctgtccggc
     1801 gacgcagtgc agaagatgct gggacactgc accttcgagc ctgcagagcc aaaggccgcc
     1861 aagaacacct acacagccga gcggtttatc tggctgacaa agctgaacaa tctgagaatc
     1921 ctggagcagg gatccgagag gccactgacc gacacagaga gggccaccct gatggatgag
     1981 ccttaccgga agtctaagct gacatatgcc caggccagaa agctgctggg cctggaggac
     2041 accgccttct ttaagggcct gagatacggc aaggataatg ccgaggcctc cacactgatg
     2101 gagatgaagg cctatcacgc catctctcgc gccctggaga aggagggcct gaaggacaag
     2161 aagtcccccc tgaacctgag ctccgagctg caggatgaga tcggcaccgc cttctctctg
     2221 tttaagaccg acgaggatat cgcaggccgc ctgaaggaca gggtgcagcc tgagatcctg
     2281 gaggccctgc tgaagaacat ctctttcgat aagtttgtgc agatcagcct gaagtccctg
     2341 agaaggatcg tgccactgat ggagcagggc aagcggtacg acgaggcctg cgccgagatc
     2401 tacggcgatc gctatggcaa gaagaacaca gaggcgaaga tctatctgcc ccctatccct
     2461 gccgacgaga tcagaaatcc tgtggtgctg agggccctgt cccagacaag aaaagtgatc
     2521 aacggagtgg tgcgccggta cggatctcca gcccggatcc acatcgagac cgccagagaa
     2581 gtgggcaaga gcttcaagga ccggaaggag atcgagaaga gacaggagga gaatcgcaag
     2641 gatcgggaga aggccgccgc caaatttagg gagtacttcc ctaattttgt gggcgagcca
     2701 aagtctaagg acatcctgaa gctgcgcctg tacgagcagc agcacggcaa gtgtctgtat
     2761 agcggcaagg agatcaatct ggtgcggctg aacgagaagg gctatgtgga gatcgatcac
     2821 gccctgcctt tctccagaac ctgggacgat tcttttaaca ataaggtgct ggtgctgggc
     2881 agcgagaacc agaataaggg caatcagaca ccatacgagt atttcaatgg caaggacaac
     2941 cccagggagt ggcaggagtt caaggcccgc gtggagacct ctagatttcc caggagcaag
     3001 aagcagcgga tcctgctgca gaaattcgac gaggatggct ttaaggagtg caacctgaat
     3061 gacaccagat acgtgagccg gttcttgtgc cagtttgtgg ccgatcacat cctgctgacc
     3121 ggcaagggca agagaagggt gttcgcctct aatggccaga tcacaaacct gctgagggga
     3181 ttttggggac tgaggaaggt gcgggcagag aatgccagac accacgcact ggatgcagtg
     3241 gtagtggcat gcagcaccgt ggcaatgcag cagaagatca caagattcgt gaggtataag
     3301 gagatgaacg cctttgacgg caagaccatc gataaggaga caggcaaggc gctgtaccag
     3361 aagacccgct tcccccagcc ttgggagttc tttgcccagg aagtgatgat ccgggtgttc
     3421 ggcaagccag acggcaagcc tgagtttgag gaggccgata ccccagagaa gctgaggaca
     3481 ctgctggcag agaagctgtc tagcaggcca gaggcagcgc acgagtacgt gaccccactg
     3541 ttcgtgtcca gggcacccaa tcggaagatg tctggcgccc acaaggccac actgagaagc
     3601 gccaagaggt ttgtgaagca caacgagaag gtctccgtga agagagtgtt gctgaccgag
     3661 atcaagctgg ccgatctgga gaacatggtg aattacaaga acggcaggga gatcgagctg
     3721 tatgaggccc tgaaggcaag gctggaggcc tacggaggaa atgccaagca ggccttcgac
     3781 ccaaaggata acccctttta taagaaggga ggacagctgg tgaaggccgt gcgggtggag
     3841 aagacccagg agagcggcgt gctgctgaat aagaagaacg cctacacaat cgccgacaat
     3901 ggcgataggg tgagagtgga cgtgttctgt aaggtggata agaagggcaa gaatcagtac
     3961 tttatcgtgc ctatctatgc ctggcaggtg gccgagaaca tcctgccaga catcgattgc
     4021 aagggctaca gaatcgacga tagctataca ttctgttttt ccctgcacag gtatgacctg
     4081 atcgccttcc agaaggatga gaagtccaag gtggagtttg cctactatat caattgcaac
     4141 gcctctaacg gctatttcta cctggcctgg cacgataagg gcagcaagga gcagcagttt
     4201 agcatctcca cccagaatct ggtgctgatc cagaagtatc aggtgagcga gctgggcaag
     4261 gagatcaggc catgtcggct gaagaagcgc ccacccgtgt ctggcggctc aaaaagaacc
     4321 gccgacggca gcgaattcga gcccaagaag aagaggaaag tctaaccggt catcatcacc
     4381 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     4441 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     4501 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     4561 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     4621 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tcgataccgt
     4681 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     4741 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctagggtg
     4801 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     4861 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     4921 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4981 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     5041 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5101 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5161 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5221 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     5281 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     5341 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     5401 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5461 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5521 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     5581 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     5641 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     5701 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5761 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     5821 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     5881 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     5941 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     6001 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     6061 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     6121 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     6181 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     6241 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     6301 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     6361 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     6421 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     6481 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     6541 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     6601 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     6661 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     6721 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     6781 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     6841 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcgatct cccgatcccc
     6901 tagggtcgac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     6961 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     7021 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     7081 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
     7141 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     7201 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     7261 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     7321 ggtaaactgc ccacttggca gtacatcaag tgtatc
//
