LOCUS       RV-dlx-FLEX-tdTomato-T2 9376 bp ds-DNA     circular     26-APR-2022
DEFINITION  .
KEYWORDS    "Plasmid name" "Resistance:Amp" "Box" "old lab code" "Addgene cat  
            no" "received from" "insert" "promoter:mDlx"
            "backbone:RV-dlx-dio-mTdt" "Author:Clara" "Sequences" "Glycerol
            stock"
FEATURES             Location/Qualifiers
     misc_recomb     200..894
                     /label="5primeLTR"
     misc_feature    1790..2653
                     /label="mdlx promoter"
     primer          2619..2642
                     /label="mDlx_fwd"
                     /note="sequence: GTGTCCACTCCCAGTTCAATTACA"
     misc_recomb     complement(2688..2712)
                     /label="Lox2772"
     CDS             2688..2796
                     /label="Seq1"
     misc_recomb     2763..2796
                     /label="LoxP"
     misc_feature    complement(2807..4528)
                     /label="T2A-SypEGFP"
     CDS             complement(2807..5962)
                     /label="Translation 5962-2807"
     misc_feature    complement(4529..5968)
                     /label="td tomato"
     CDS             5977..6097
                     /label="Seq1"
     misc_recomb     5980..6012
                     /label="Lox2772"
     misc_recomb     complement(6064..6097)
                     /label="LoxP"
     misc_signal     6109..6684
                     /label="WPRE"
     misc_feature    complement(6163..6184)
                     /label="Rev_WRE"
     misc_feature    6768..7361
                     /label="3'-LTR"
     rep_origin      complement(7715..8397)
                     /label="ColE1 origin"
     CDS             complement(8502..9161)
                     /label="AmpR"
ORIGIN
        1 GATCCGGCCA TTAGCCATAT TATTCATTGG TTATATAGCA TAAATCAATA TTGGCTATTG
       61 GCCATTGCAT ACGTTGTATC CATATCATAA TATGTACATT TATATTGGCT CATGTCCAAC
      121 ATTACCGCCA TGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC
      181 ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC
      241 TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT
      301 AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA
      361 CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG
      421 TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA
      481 GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA
      541 TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA
      601 TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC
      661 CCCATTGACG CAAATGGGCG GTAGGCATGT ACGGTGGGAG GTCTATATAA GCAGAGCTCA
      721 ATAAAAGAGC CCACAACCCC TCACTCGGGG CGCCAGTCCT CCGATTGACT GAGTCGCCCG
      781 GGTACCCGTA TTCCCAATAA AGCCTCTTGC TGTTTGCATC CGAATCGTGG TCTCGCTGTT
      841 CCTTGGGAGG GTCTCCTCTG AGTGATTGAC TACCCACGAC GGGGGTCTTT CATTTGGGGG
      901 CTCGTCCGGG ATTTGGAGAC CCCTGCCCAG GGACCACCGA CCCACCACCG GGAGGTAAGC
      961 TGGCCAGCAA CTTATCTGTG TCTGTCCGAT TGTCTAGTGT CTATGTTTGA TGTTATGCGC
     1021 CTGCGTCTGT ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC
     1081 GAGTTCTGAA CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTTGG GGGCCGTTTT
     1141 TGTGGCCCGA CCTGAGGAAG GGAGTCGATG TGGAATCCGA CCCCGTCAGG ATATGTGGTT
     1201 CTGGTAGGAG ACGAGAACCT AAAACAGTTC CCGCCTCCGT CTGAATTTTT GCTTTCGGTT
     1261 TGGAACCGAA GCCGCGCGTC TTGTCTGCTG CAGCGCTGCA GCATCGTTCT GTGTTGTCTC
     1321 TGTCTGACTG TGTTTCTGTA TTTGTCTGAA AATTAGGGCC AGACTGTTAC CACTCCCTTA
     1381 AGTTTGACCT TAGGTCACTG GAAAGATGTC GAGCGGATCG CTCACAACCA GTCGGTAGAT
     1441 GTCAAGAAGA GACGTTGGGT TACCTTCTGC TCTGCAGAAT GGCCAACCTT TAACGTCGGA
     1501 TGGCCGCGAG ACGGCACCTT TAACCGAGAC CTCATCACCC AGGTTAAGAT CAAGGTCTTT
     1561 TCACCTGGCC CGCATGGACA CCCAGACCAG GTCCCCTACA TCGTGACCTG GGAAGCCTTG
     1621 GCTTTTGACC CCCCTCCCTG GGTCAAGCCC TTTGTACACC CTAAGCCTCC GCCTCCTCTT
     1681 CCTCCATCCG CCCCGTCTCT CCCCCTTGAA CCTCCTCGTT CGACCCCGCC TCGATCCTCC
     1741 CTTTATCCAG CCCTCACTCC TTCTCTAGGC GCCGGAATTC GATGTCGACG AATTCTATAC
     1801 ACTCACAGtg gtttggcata tatttggtga aattttttaa ggaaaaatta gtgttggttt
     1861 cgatatatgg tagctttttc tctaacataa tttgaataat tcagcaaagc cctactacca
     1921 gctgtacttc tgcagcctct tccattcttt ccagcattat aattttggtt aattttcaat
     1981 tttaggtcct acgtctctgc aatttgtgta tgaataacag aataatttcc ctcttttgtt
     2041 tcgcctttcc tgttcctgaa tctaaataaa gatggctttt tagtattaaa agtggaagaa
     2101 aattacaggt aattatcttt gacggtaaaa acgctgtaat cagcgggcta catgaaaaat
     2161 tactctaatt atggctgcat ttaagagaat ggaaaaaaac cttcttgtgg ataaaaacct
     2221 taaattgtcc ccaatgtctg cttcaaattg gatggcactg cagctggagg ctttgttcag
     2281 aattgatcct ggggagctac gaacccaaag tttcacagta ggaagcttga tatcgaattc
     2341 ctgcagcccg ggctgggcat aaaagtcagg gcagagccat ctattgctta catttgcttc
     2401 tagcctgcag gtcgaggagc gcagccttcc agaagcagag cgcggcgcct taagctgcag
     2461 aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga
     2521 ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat
     2581 tggtcttact gacatccact ttgcctttct ctccacaggt gtccactccc aGTTCAATTA
     2641 CAGCTCTTAA GAATTCGAAA TATAACATCG GATCCGGAAT AACTTCGTAT AGGATACTTT
     2701 ATACGAAGTT ATGCAGAATG GTAGCTGGAT TGTAGCTGCT ATTAGCAATA TGAAACCTCT
     2761 TAATAACTTC GTATAGCATA CATTATACGA AGTTATTTAA TTAAGCttac ttgtacagct
     2821 cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc
     2881 gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg ttgtcgggca
     2941 gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc
     3001 tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttgt
     3061 cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt
     3121 tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct
     3181 cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct
     3241 cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg
     3301 ggtagcggct gaagcactgc acgccgtagg tcagggtggt cacgagggtg ggccagggca
     3361 cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc
     3421 cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca
     3481 ggatgggcac caccccggtg aacagctcct cgcccttgct caccattcgc gacatctgat
     3541 tggagaagga ggtgggcgca ccctgttggc cgtagccttg ctgcccatag tcgccctgag
     3601 gcccgtagcc accgccaccg ccgctggctg gctgcccgta atcgggttga taaccaccct
     3661 gaggcccgta ggagtcctgg ggcccatagc ctccggggcc ctgcccatag cccgcatcgc
     3721 cgtaggcatc gccaggagct ggttgctttt ctggggcgcc tggaggtgcg cgcatgaatg
     3781 gggcggccca gcctgtctcc ttgaacacga accataggtt gccaacccag agcaccaggt
     3841 tcaggaagcc aaacaccacc gaggtgttga gtcctgaagt cacagggtcc ctcagttcct
     3901 tgcatgtgtt tcctgtctgg cggcacatag gcatctcctt gataatgttc tctgggtcag
     3961 tggccatctt cacatcggac aggcctttgg cccaggcgga tgagctaact agccacatga
     4021 aagcgaacac tgctgtggcc aggaagtcca tcattggccc tttgttgttc tctcggtact
     4081 tgttctgcag gaagatgtag gtggccaggg cccccatgga gtagaggaag gcaaacacag
     4141 ccacggtgac aaagaattca gccgaggagg agtagtcacc aactaggaag atcttggtag
     4201 tgcccccttt aacgcaggag ggtgcatcaa agtacacttg gtgcagcctg aatgggtact
     4261 caaattcgac ttcgatgttg agggcactct ccgtcttgtt ggcacactcc acgctcagcc
     4321 gaagctctcc ggtgtagctg ccgcacgtag caaaggcgaa gatggcaaag acccactgca
     4381 gcaccttcac gaagccaagg ggctccttga ccacccggaa ctgaccccca gccaccagct
     4441 gattcaccac gtccattcta gatgagcctg gggcagggcc gggattctct tccacgtctc
     4501 cgcatgtcag aagacttcct ctgccctcac tagtcttgta cagctcgtcc atgccgtaca
     4561 gaaacagatg gtgcctcccc tcgctccgct catactgctc cacaatggtg tagtcttcgt
     4621 tatggctagt aatatccagc tttgtgtcga cgtaatagta gccaggcagc tgcacgggtt
     4681 tcttggccat gtagatggtt ttgaattcga ccagataatg ccctccatct ttcagcttca
     4741 gtgcctggtg gatctctccc ttcagcacgc cgtctctggg gtacagcctt tctgttgaag
     4801 cctcccagcc cattgtcttc ttctgcatga ctgggccatc agggggaaaa ttggtgccgc
     4861 gcattttcac cttatagatc agagtgccgt cctgcagaga gctgtcctgg gtgacagtca
     4921 ccaggccccc gtcttcgaag ttcatcactc tttcccactt aaacccctct gggaagctca
     4981 gtttcttgta atctggaatg tcagcagggt gtttcacgta ggctttactt ccatacataa
     5041 actgaggaga caggatgtcc catgcgaatg gcagagggcc gccctttgtc actttcagct
     5101 ttgctgtctg ggtgccctca tagggcctcc cttccccctc tccttcaatc tcaaactcgt
     5161 gcccgttcat gctgccctcc atcctgactt taaacctcat aaactcctta atcacggcca
     5221 tgttgttgtc ctcggaggag gcggtgccgg agctgccgct gccggtgctg ccggtaccat
     5281 gccccaggaa caggtggtgg cggccctcgg agcgctcgta ctgttccacg atggtgtagt
     5341 cctcgttgtg ggaggtgatg tccagcttgg tgtccacgta gtagtagccg ggcagttgca
     5401 cgggcttctt ggccatgtag atggtcttga actccaccag gtagtggccg ccgtccttca
     5461 gcttcagggc ctggtggatc tcgcccttca gcacgccgtc gcgggggtac aggcgctcgg
     5521 tggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcgggg gggaagttgg
     5581 tgccgcgcat cttcaccttg tagatcagcg tgccgtcctg cagggaggag tcctgggtca
     5641 cggtcaccag accgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga
     5701 aggacagctt cttgtaatcg gggatgtcgg cggggtgctt cacgtacgcc ttggagccgt
     5761 acatgaactg gggggacagg atgtcccagg cgaagggcag ggggccgccc ttggtcacct
     5821 tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc tcgatctcga
     5881 actcgtggcc gttcatggag ccctccatgc gcaccttgaa gcgcatgaac tctttgatga
     5941 cctcctcgcc cttgctcacc atactagtGG CGCGCCGCGA TAACTTCGTA TAAAGTATCC
     6001 TATACGAAGT TATTTGCCTT AACCCAGAAA TTATCACTGT TATTCTTTAG AATGGTGCAA
     6061 AGAATAACTT CGTATAATGT ATGCTATACG AAGTTATGTT TAAACCAATC AACCTCTGGA
     6121 TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG
     6181 TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT
     6241 CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG
     6301 GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC
     6361 CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA
     6421 ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA
     6481 TTCCGTGGTG TTGTCGGGGA AGCTGACGTC CTTTCCATGG CTGCTCGCCT GTGTTGCCAC
     6541 CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT
     6601 TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA
     6661 GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCCTGGA ATTCGAGCTC GAGCTTGTTA
     6721 ACATCGATAA AATAAAAGAT TTTATTTAGT CTCCAGAAAA AGGGGGGAAT GAAAGACCCC
     6781 ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT GCAAGGCATG GAAAAATACA
     6841 TAACTGAGAA TAGAGAAGTT CAGATCAAGG TCAGGAACAG ATGGAACAGC TGAATATGGG
     6901 CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT CAGGGCCAAG AACAGATGGA
     6961 ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA GTTCCTGCCC CGGCTCAGGG
     7021 CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC AGTTTCTAGA GAACCATCAG
     7081 ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG CCTTATTTGA ACTAACCAAT
     7141 CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC GAGCTCAATA AAAGAGCCCA
     7201 CAACCCCTCA CTCGGGGCGC CAGTCCTCCG ATTGACTGAG TCGCCCGGGT ACCCGTGTAT
     7261 CCAATAAACC CTCTTGCAGT TGCATCCGAC TTGTGGTCTC GCTGTTCCTT GGGAGGGTCT
     7321 CCTCTGAGTG ATTGACTACC CGTCAGCGGG GGTCTTTCAT TTCCGACTTG TGGTCTCGCT
     7381 GCCTTGGGAG GGTCTCCTCT GAGTGATTGA CTACCCGTCA GCGGGGGTCT TCACATGCAG
     7441 CATGTATCAA AATTAATTTG GTTTTTTTTC TTAAGTATTT ACATTAAATG GCCATAGTTG
     7501 CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGCGC TCTTCCGCTT
     7561 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT
     7621 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG
     7681 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA
     7741 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
     7801 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
     7861 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
     7921 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
     7981 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
     8041 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
     8101 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
     8161 GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
     8221 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
     8281 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT
     8341 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT
     8401 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTG CGGCCGCAAA
     8461 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG
     8521 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG
     8581 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA
     8641 GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG
     8701 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA
     8761 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC
     8821 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA
     8881 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG
     8941 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT
     9001 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC
     9061 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAACACGG
     9121 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG
     9181 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT
     9241 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA
     9301 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA
     9361 CTCTTCCTTT TTCAAT
//