LOCUS       Exported                9531 bp DNA     circular SYN 26-APR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 26, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             910..930
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             937..4956
                     /codon_start=1
                     /label=anCas(BCA)
                     /note="color: #339966"
                     /translation="MKKDYSIGLDIGTNSVGWAVITDDYKVVRKKMKVLGNTDKKSIKK
                     NLWGVRLFDSGETAEDTRLKRTTRRRYTRRRNRICYLQEIFQEEMNKVDDSFFHRLDES
                     FLVPEDKKYDRHPIFGNLEEEVAYHEQYPTIYHLRKHLADSSEKADLRLVYLALAHIIK
                     YRGHFLIEGDLNTENTDVEELFKQFVQVYNQTFEEQHLSDETIDVEEILTEKVSKSRRA
                     ENVLKLFPNEKKNGLFGQLIKLIVGLQGNFKKVFDLSEDAKLQFSKDTYEEDLENLLAM
                     IGDEYADLFLAAKNLYDAILLSGILTTTDNNTKAKLSASMIKRYEEHQKDLAQLKQFIK
                     EHLPDKYNEIFSDSSKNGYAGYIEGKTSQEDFYKYLKKILSKIDGAEYFLEKIEQENFL
                     RKQRTFDNGVIPHQIHLEELRAILRRQGKYYPFLKENQEKIEQILTFRIPYYVGPLARG
                     NSRFAWLTRKSDEPITPWNFDEVVDKEKSAEAFIERMTNYDTYLPNEKVLPKHSLLYEK
                     FTVFNELTKVRYVTDRGKTQNFSAEQKQEIFDDLFKKNRKVTKKKLENYLKKEYEYFDS
                     PDITGIEDEFNASLGTYHDLLKILKSKDFLDDEENEEILEDIVKILTVFEDRKMIRKRL
                     EKYSDILTEEQLKKLERRHYTGWGRLSRKLINGIRDKQSGKTILDYLIDDDSSNRNFMQ
                     LINDDNLSFKEEIEKAQVIGETENLHEIVQDLPGSPAIKKGILQSLKIVDELVKVMGHE
                     PKNIVVEMARENQTTSRGRRNSKQRLKRLEEALKNLGSNLLKEHPVDNQQLQNDRLYLY
                     YLQNGKDMYTGQELDIDNLSNYDIDHIIPQSFIKDNSIDNRVLVSSEENRGKSDNVPSK
                     EVVRKMKSFWQKLLNAGLISQRKFDNLTKAERGGLTEDDKAGFIKRQLVETRQITKHVA
                     NILDSRFNTEKDEEDNTIRNVKIITLKSNLVSQFRKDFGLYKVREINDYHHAHDAYLNA
                     VVGTALLKKYPQLEPEFVYGDYRKNNAREENKATAKKHFYSNIMNFFASEVKIADETGE
                     IVWDKEKDIATVRKVLSYHQVNIVKKVEVQKGGFSKETILPKGNSNKLIPRKNNWDPKK
                     YGGFDSPTVAYSVLVTYDIEKGKKKKLKTVKELVGITIMERSAFEKNPIAYLEKKGYQN
                     PQEDVLIKLPKYSLFELENGRRRMLASAKELQKGNQMVLPAHLVTLLYHAKRIDKSNNS
                     ESLEYVEEHRNEFDELLDYIIDFSEKYILADKNLEKIKKLYEQNNEADIKELAKSFINL
                     LTFTAMGAPAAFKFFGETIDRKRYTSIKELLNATLIHQSITGLYETRIDLGKLGED"
     CDS             4957..4974
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     CDS             4993..5013
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     polyA_signal    5128..5352
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      5398..5826
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        5840..6169
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      6020..6155
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             6236..7030
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    7204..7325
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(7374..7390)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    7398..7414
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(7422..7452)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 7422 .. 7428 / #ffffff / -10
                        2: 7429 .. 7446 / #ffffff
                        3: 7447 .. 7452 / #ffffff / -35"
     protein_bind    7467..7488
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(7776..8364)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(8535..9395)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 8535 .. 9326 / #ccffcc
                        2: 9327 .. 9395 / #ccffcc / signal sequence
                     Cleavage site after base 9326"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9396..9500)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccaccatgc ccaagaaaaa gcggaaggtg cccaagatga agaaggacta ttctatcgga
      961 ctggacatcg gcacaaacag cgtgggctgg gccgtgatca cagacgacta caaggtcgtg
     1021 cgcaagaaaa tgaaggtgct gggcaacacc gataaaaaga gcatcaagaa aaacctgtgg
     1081 ggagtgagac tgttcgactc tggcgaaacc gccgaggata caagactgaa gcgaaccacc
     1141 agacggagat acaccagaag aagaaaccgc atctgctacc tgcaggaaat cttccaggaa
     1201 gagatgaaca aggtcgacga cagcttcttc caccgcctgg atgaaagctt cctggtgccc
     1261 gaggataaaa agtacgacag acaccctatc tttggcaacc tggaagaaga agtggcctac
     1321 catgagcaat accctaccat ctaccacctg cggaagcacc tcgccgattc tagtgagaag
     1381 gccgatctga gactggttta cctggccctg gcccatatca tcaaatacag aggccacttc
     1441 ctgatcgaag gcgacctgaa caccgagaac actgacgtgg aagagctgtt caaacagttc
     1501 gtgcaggtgt acaaccagac cttcgaggaa caacacctgt cggatgagac catcgacgtg
     1561 gaggaaatcc tgacagaaaa agtgtccaag agcagacggg ccgagaacgt gctgaagcta
     1621 tttccaaatg agaagaaaaa cggcctgttt ggccagctga tcaagctgat cgttggcctg
     1681 cagggcaact tcaagaaagt gttcgacctc agcgaggacg ccaagctcca attttctaag
     1741 gatacctatg aggaggacct ggaaaacctt ctggccatga tcggggacga gtacgccgac
     1801 ctgttcctgg ccgccaaaaa cctgtacgac gctatcctgc tttctggaat cctgaccaca
     1861 accgataata acaccaaggc caagctgagc gccagcatga tcaagcggta cgaggagcac
     1921 cagaaggacc tggcacaact gaagcagttc atcaaagagc acctgccaga caagtacaat
     1981 gaaatcttca gcgactccag caaaaacggc tatgccggct acatcgaggg caaaaccagc
     2041 caggaggatt tctacaagta cctgaagaag atcctgtcta agattgatgg cgctgaatac
     2101 ttcctggaga aaattgaaca ggaaaatttt ctgaggaagc aaagaacctt tgacaacggc
     2161 gtgatcccgc accagatcca cctggaggag ctgagagcca tcctgaggcg tcagggcaag
     2221 tactacccat tcctcaaaga gaaccaggag aagatagagc agatcctgac atttagaatc
     2281 ccctactacg tgggcccact ggctagagga aatagccggt tcgcctggct gacacggaag
     2341 tctgacgagc ccatcacacc ttggaacttc gacgaagtcg tggacaagga gaagtccgcc
     2401 gaggccttca tcgagagaat gaccaactac gacacctacc tgcctaacga aaaggtactg
     2461 cccaaacaca gcctgctgta cgagaagttc accgtgttca acgaattgac aaaggttcgg
     2521 tacgtgacag accggggcaa gacccagaac ttctccgccg agcagaagca ggagatcttc
     2581 gacgacctgt tcaagaagaa ccggaaggtc acaaagaaaa agctggagaa ctatctgaag
     2641 aaagaatacg agtacttcga cagccctgat atcaccggaa ttgaggacga gttcaacgcc
     2701 agcctgggca cctatcatga tctgctgaag atcctgaagt ctaaggactt cctggacgat
     2761 gaggaaaacg aggaaatcct ggaggatatc gtgaagatcc tgaccgtgtt cgaggacaga
     2821 aagatgatca gaaagcggct ggagaaatac agcgacatcc tcaccgagga acagttgaag
     2881 aagctggagc ggcggcacta cacaggctgg ggcagattga gcagaaagct tatcaacggc
     2941 atccgcgaca aacagagcgg caagacaatt ttagattacc tgatcgacga cgacagcagt
     3001 aaccggaact tcatgcagct gatcaacgat gacaatttga gctttaagga ggaaatcgag
     3061 aaggcccagg tgatcggaga gaccgagaac ctgcacgaga tagtgcaaga cctgcctggc
     3121 agccctgcta tcaagaaggg catcctgcag tctctgaaga ttgtggacga gctcgtgaag
     3181 gtgatggggc acgaacctaa gaacatcgtg gtcgaaatgg ccagagagaa ccagacgacc
     3241 agcaggggca gacggaacag caagcagcgg ctgaagagac tggaggaggc tcttaagaac
     3301 ctgggcagca acctgctgaa agaacacccc gtggacaatc agcagctgca gaacgacaga
     3361 ctgtacctat actacctgca aaatggcaag gatatgtaca caggacagga gctggacatc
     3421 gataatctgt ccaactacga catcgatcac atcatccccc agagtttcat caaggataat
     3481 agcatcgata accgagtgct ggtgagcagc gaggaaaata gaggcaagtc tgataatgtg
     3541 ccttctaagg aagttgtgag aaagatgaaa tctttttggc agaaactgct gaatgctggc
     3601 ctgatcagcc agagaaaatt cgacaacctg acaaaggctg agagaggcgg cctgaccgaa
     3661 gacgacaaag ctggattcat caagagacag ctggtggaaa caagacagat caccaagcac
     3721 gtggccaaca tcctagacag ccggttcaac acagagaagg acgaggagga caacaccatc
     3781 agaaacgtga agattatcac cctgaagtcc aacctggtgt cccagttcag aaaggatttc
     3841 ggcctgtaca aggttcggga aattaacgac taccaccacg cccacgacgc ctacctgaat
     3901 gccgtggtgg gcaccgccct gctgaagaag tatcctcaac tggagcctga gttcgtgtac
     3961 ggcgactacc ggaagaacaa cgccagagag gaaaacaagg ccacagccaa gaagcacttc
     4021 tacagcaata tcatgaactt cttcgcctct gaagtgaaga ttgccgacga aaccggcgag
     4081 atcgtgtggg acaaagaaaa ggacatagct accgtgcgga aggtgctgtc ctaccaccag
     4141 gtgaacatcg tgaagaaagt ggaggtgcag aagggaggat tctccaagga aaccatcctc
     4201 cccaagggca acagcaacaa gctcatcccc agaaagaaca actgggaccc taagaagtac
     4261 ggcggcttcg acagccccac cgtggcttat agcgtgctgg tgacctacga tatcgagaag
     4321 ggaaagaaaa agaagctgaa gaccgtgaaa gagctggtcg gcatcaccat catggagcgg
     4381 agcgcctttg aaaagaaccc catcgcctac ctggaaaaaa aaggctacca gaatcctcag
     4441 gaggacgtgc tgatcaaact gcctaagtac tccctgttcg agctggaaaa cggcagacgt
     4501 cggatgctgg ccagcgctaa ggaactccaa aagggcaatc agatggtgct tcctgcccat
     4561 ctggtgaccc tgctgtacca cgccaaacgg atcgataaga gcaacaattc tgagagcctg
     4621 gaatacgtgg aagaacacag aaatgaattc gacgagctgc tggactatat cattgatttc
     4681 agcgagaagt atatcctggc tgataagaac ctggaaaaga tcaagaagct ttacgagcag
     4741 aacaacgagg ccgacatcaa ggagctggcc aagagcttta tcaacctgct gaccttcacc
     4801 gccatgggcg cccctgccgc tttcaagttc ttcggcgaaa caatcgacag aaaaagatac
     4861 acaagcatca aggaactgct caacgccacc ctcatccacc agagcatcac aggtctgtac
     4921 gagacaagaa tcgacctggg caagctggga gaggatcacc atcatcatca ccacgcctct
     4981 ggaacaggcg gacctaaaaa gaagaggaag gtttaaggta ccgagctcgg atccactagt
     5041 ccagtgtggt ggaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc
     5101 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     5161 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     5221 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     5281 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     5341 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     5401 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     5461 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     5521 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     5581 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     5641 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     5701 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     5761 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     5821 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     5881 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     5941 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     6001 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6061 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     6121 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     6181 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     6241 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     6301 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     6361 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     6421 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     6481 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     6541 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     6601 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     6661 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     6721 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     6781 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     6841 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     6901 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     6961 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     7021 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     7081 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     7141 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     7201 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     7261 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7321 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     7381 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7441 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7501 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7561 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7621 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7681 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7741 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7801 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7861 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7921 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7981 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8041 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8101 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8161 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     8221 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8281 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8341 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8401 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8461 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8521 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8581 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8641 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8701 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8761 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8821 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8881 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8941 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9001 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9061 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9121 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     9181 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9241 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9301 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9361 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9421 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9481 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
