LOCUS       Exported               11323 bp ds-DNA     circular SYN 28-APR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Apr 28, 2022 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11323
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        191..570
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        571..774
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     725..745
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
                     /note="color: black; sequence: CGCAAATGGGCGGTAGGCGTG"
     LTR             788..968
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    1015..1140
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1633..1866
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    2393..2510
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    2564..3748
                     /label=Ubc promoter
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     3611..3630
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
                     /note="color: black; sequence: TGAAGCTCCGGTTTTGAACT"
     regulatory      3847..3856
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     CDS             3853..4569
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 3853 .. 3855 / #05fd14
                        2: 3856 .. 3858 / #05fd14 / 1a
                        3: 3859 .. 4569 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3898..3919)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
                     /note="color: black; sequence: CGTCGCCGTCCAGCTCGACCAG"
     primer_bind     complement(4159..4178)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
                     /note="color: black; sequence: GTCTTGTAGTTGCCGTCGTC"
     primer_bind     4506..4527
                     /label=EGFP-C
                     /note="EGFP, forward primer"
                     /note="color: black; sequence: CATGGTCCTGCTGGAGTTCGTG"
     primer_bind     4560..4597
                     /label=BsrGI-2A-F
                     /note="color: black; sequence: 
                     gctgtacaagGCGCGCCaaattgtcgctcctgtcaaac; added: 2021-06-23"
     primer_bind     4560..4577
                     /label=BsrGI-AscI-F
                     /note="color: black; sequence: gctgtacaagGCGCGCCa; added: 
                     2021-06-23"
     CDS             4591..4656
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 4653"
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     primer_bind     complement(4636..4670)
                     /label=NheI-2A-R
                     /note="color: black; sequence: 
                     actggcatGCTAGCtggacctggattgctttctac; added: 2021-06-23"
     primer_bind     4654..4680
                     /label=NheI-BoNT-F
                     /note="color: black; sequence: ccaGCTAGCatgccagttacaataaat;
                     added: 2021-06-23"
     CDS             4663..5985
                     /codon_start=1
                     /label=BoNT/B light chain
                     /label=BoNT/B\light\chain
                     /label=nonstandard type: allele
                     /note="color: #9eafd2"
                     /translation="MPVTINNFNYNDPIDNNNIIMMEPPFARGTGRYYKAFKITDRIWI
                     IPERYTFGYKPEDFNKSSGIFNRDVCEYYDPDYLNTNDKKNIFLQTMIKLFNRIKSKPL
                     GEKLLEMIINGIPYLGDRRVPLEEFNTNIASVTVNKLISNPGEVERKKGIFANLIIFGP
                     GPVLNENETIDIGIQNHFASREGFGGIMQMKFCPEYVSVFNNVQENKGASIFNRRGYFS
                     DPALILMHELIHVLHGLYGIKVDDLPIVPNEKKFFMQSTDAIQAEELYTFGGQDPSIIT
                     PSTDKSIYDKVLQNFRGIVDRLNKVLVCISDPNININIYKNKFKDKYKFVEDSEGKYSI
                     DVESFDKLYKSLMFGFTETNIAENYKIKTRASYFSDSLPPVKIKNLLDNEIYTIEEGFN
                     ISDKDMEKEYRGQNKAINKQAYEEISKEHLAVYKIQMCKSVK"
     primer_bind     complement(5088..5109)
                     /label=BoNT-SEQ
                     /note="color: black; sequence: ctccacttctcctggattactg; 
                     added: 2021-07-21"
     primer_bind     complement(5206..5224)
                     /label=BoNT-seq-R
                     /note="color: black; sequence: cgaagccttcccttgatgc; added: 
                     2021-07-21"
     primer_bind     complement(5965..5996)
                     /label=EcoRI-BoNT-R
                     /note="color: black; sequence: 
                     tcgaattcttatttaacacttttacacatttg; added: 2021-06-23"
     misc_feature    6013..6601
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     primer_bind     complement(6066..6086)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
                     /note="color: black; sequence: CATAGCGTAAAAGGAGCAACA"
     CDS             complement(6484..6495)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 6483"
                     /translation="IEGR"
     primer_bind     complement(6604..6620)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(6605..6621)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
                     /note="color: black; sequence: TCGAGGTCGACGGTATC"
     LTR             7126..7306
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     complement(7332..7349)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
                     /note="color: black; sequence: TAGAAGGCACAGTCGAGG"
     polyA_signal    7338..7562
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      7608..8036
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     complement(7695..7714)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
                     /note="color: black; sequence: AGGGAAGAAAGCGAAAGGAG"
     primer_bind     7905..7926
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
                     /note="color: black; sequence: GTGGACTCTTGTTCCAAACTGG"
     primer_bind     complement(8045..8065)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
                     /note="color: black; sequence: ACCCTAACTGACACACATTCC"
     promoter        8050..8379
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      8230..8365
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     primer_bind     8292..8311
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
                     /note="color: black; sequence: TATTTATGCAGAGGCCGAGG"
     promoter        8427..8474
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
                     /note="color: #ffffff; direction: RIGHT"
     CDS             8493..8867
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /note="color: #ccffcc"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8997..9118
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(9034..9053)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
                     /note="color: black; sequence: GAAATTTGTGATGCTATTGC"
     primer_bind     9088..9107
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC"
     primer_bind     complement(9167..9183)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(9167..9183)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     primer_bind     complement(9180..9202)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGG"
     protein_bind    9191..9207
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(9215..9245)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 9215 .. 9221 / #ffffff / -10
                        2: 9222 .. 9239 / #ffffff
                        3: 9240 .. 9245 / #ffffff / -35"
     protein_bind    9260..9281
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     complement(9398..9415)
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     rep_origin      complement(9569..10157)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(9649..9668)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     CDS             complement(10328..11188)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 10328 .. 11119 / #ccffcc
                        2: 11120 .. 11188 / #ccffcc / signal sequence
                     Cleavage site after base 11119"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10951..10970
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     promoter        complement(11189..11293)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
       61 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
      121 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
      181 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
      241 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      301 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      361 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      421 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      481 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      541 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      601 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      661 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      721 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc
      781 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
      841 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      901 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      961 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     1021 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     1081 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     1141 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     1201 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     1261 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     1321 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     1381 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     1441 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     1501 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1561 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1621 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1681 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1741 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1801 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1861 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1921 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1981 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     2041 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     2101 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     2161 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     2221 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     2281 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     2341 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     2401 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2461 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2521 acagggacag cagagatcca gtttggttaa ttaagggtgc agcggcctcc gcgccgggtt
     2581 ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg
     2641 cgcaggagcg ttcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa
     2701 gactcggcct tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac
     2761 tctagggcac tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc
     2821 ggcgattctg cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg
     2881 ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg
     2941 gatcgctgtg atcgtcactt ggtgagttgc gggctgctgg gctggccggg gctttcgtgg
     3001 ccgccgggcc gctcggtggg acggaagcgt gtggagagac cgccaagggc tgtagtctgg
     3061 gtccgcgagc aaggttgccc tgaactgggg gttgggggga gcgcacaaaa tggcggctgt
     3121 tcccgagtct tgaatggaag acgcttgtaa ggcgggctgt gaggtcgttg aaacaaggtg
     3181 gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct
     3241 cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa gtttgtcact
     3301 gactggagaa ctcgggtttg tcgtctggtt gcgggggcgg cagttatgcg gtgccgttgg
     3361 gcagtgcacc cgtacctttg ggagcgcgcg cctcgtcgtg tcgtgacgtc acccgttctg
     3421 ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc ttttctccgt
     3481 cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc gccggacctc
     3541 tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg tacctatctt
     3601 cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag tgtgttttgt
     3661 gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt
     3721 tagactagta aagcttctgc aggtcgactc tagaaaattg tccgctaaat tctggccgtt
     3781 tttggctttt ttgttagacg aagcttgggc tgcaggtcga ctctagagga tccccgggta
     3841 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     3901 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     3961 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     4021 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     4081 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     4141 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     4201 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     4261 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     4321 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     4381 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     4441 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     4501 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     4561 ctgtacaagg cgcgccaaat tgtcgctcct gtcaaacaaa ctcttaactt tgatttactc
     4621 aaactggctg gggatgtaga aagcaatcca ggtccagcta gcatgccagt tacaataaat
     4681 aattttaatt ataatgatcc tattgataat aataatatta ttatgatgga gcctccattt
     4741 gcgagaggta cggggagata ttataaagct tttaaaatca cagatcgtat ttggataata
     4801 ccggaaagat atacttttgg atataaacct gaggatttta ataaaagttc cggtattttt
     4861 aatagagatg tttgtgaata ttatgatcca gattacttaa atactaatga taaaaagaat
     4921 atatttttac aaacaatgat caagttattt aatagaatca aatcaaaacc attgggtgaa
     4981 aagttattag agatgattat aaatggtata ccttatcttg gagatagacg tgttccactc
     5041 gaagagttta acacaaacat tgctagtgta actgttaata aattaatcag taatccagga
     5101 gaagtggagc gaaaaaaagg tattttcgca aatttaataa tatttggacc tgggccagtt
     5161 ttaaatgaaa atgagactat agatataggt atacaaaatc attttgcatc aagggaaggc
     5221 ttcgggggta taatgcaaat gaagttttgc ccagaatatg taagcgtatt taataatgtt
     5281 caagaaaaca aaggcgcaag tatatttaat agacgtggat atttttcaga tccagccttg
     5341 atattaatgc atgaacttat acatgtttta catggattat atggcattaa agtagatgat
     5401 ttaccaattg taccaaatga aaaaaaattt tttatgcaat ctacagatgc tatacaggca
     5461 gaagaactat atacatttgg aggacaagat cccagcatca taactccttc tacggataaa
     5521 agtatctatg ataaagtttt gcaaaatttt agagggatag ttgatagact taacaaggtt
     5581 ttagtttgca tatcagatcc taacattaat attaatatat ataaaaataa atttaaagat
     5641 aaatataaat tcgttgaaga ttctgaggga aaatatagta tagatgtaga aagttttgat
     5701 aaattatata aaagcttaat gtttggtttt acagaaacta atatagcaga aaattataaa
     5761 ataaaaacta gagcttctta ttttagtgat tccttaccac cagtaaaaat aaaaaattta
     5821 ttagataatg aaatctatac tatagaggaa gggtttaata tatctgataa agatatggaa
     5881 aaagaatata gaggtcagaa taaagctata aataaacaag cttatgaaga aattagcaag
     5941 gagcatttgg ctgtatataa gatacaaatg tgtaaaagtg ttaaataaga attcgatatc
     6001 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6061 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6121 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6181 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     6241 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     6301 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     6361 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     6421 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     6481 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     6541 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     6601 catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca
     6661 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     6721 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     6781 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     6841 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     6901 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     6961 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     7021 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     7081 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     7141 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7201 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7261 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     7321 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     7381 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     7441 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     7501 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     7561 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     7621 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     7681 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     7741 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     7801 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     7861 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     7921 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     7981 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     8041 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     8101 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     8161 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     8221 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8281 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     8341 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     8401 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     8461 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     8521 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     8581 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     8641 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     8701 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     8761 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     8821 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
     8881 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     8941 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     9001 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     9061 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     9121 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     9181 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     9241 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     9301 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     9361 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     9421 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     9481 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     9541 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     9601 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     9661 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9721 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9781 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9841 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9901 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9961 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
    10021 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    10081 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    10141 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    10201 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    10261 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    10321 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    10381 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    10441 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    10501 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    10561 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    10621 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    10681 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    10741 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    10801 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    10861 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    10921 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    10981 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    11041 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    11101 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    11161 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    11221 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    11281 taggggttcc gcgcacattt ccccgaaaag tgccacctga cac
//
